Progress 01/01/24 to 12/31/24
Outputs
Target Audience:Primary audiences include graduate students, food scientists, and the food industry. It is important to share our findings and apply this technique in the food supply chain. Thus, primary audiences will be reached through interactions at local and national conferences, as well as open access to scientific publications. Collaborations with other food scientists and the food industry will be another way to approach the primary audiences. In addition, we will train our graduate students who will rise to the challenges of food safety and food security in the coming years. To distribute our findings, the developed detection method will be shared with extension educators via direct email/phone communications. Agricultural growers and producers will be reached via extension agents. Additional means of dissemination of our findings include a training-the-trainer program, extension publications, and presentations at the Southern Fruit/Vegetable Conference or similar state/regional/national venues. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?1) Two undergraduate students (John Zhao and Merna Amine, senior year in the Department of Bioengineering and Biological Science at the University of California Riverside) started to clone the norovirus-specific nanobody-display vectors and displayed these nanobodies on yeast surface. 2) One graduate student (Zilong Liu, a second-year Ph.D. student in the Department of Bioengineering at the University of California Riverside) gave one poster presentation in the Department of Bioengineering at the University of California Riverside. The student was provided feedback by the communities of food safety and bioengineering. How have the results been disseminated to communities of interest?One poster presentation (Zilong Liu) orally presented research findings for this project to an audience of biologists and bioengineers in the Department of Bioengineering at the University of California Riverside. What do you plan to do during the next reporting period to accomplish the goals?1) Publish 1-2 peer-reviewed papers. 2) Present findings at meetings (including the International Association of Food Protection, Gordon Research Conference, and American Chemical Society) to share the results with food scientists and bioengineers who are working on food safety. 3) Work with undergraduate students, graduate students, and stakeholders in California to develop a better understanding of the impacts of this study.
Impacts
What was accomplished under these goals?
In this period, weamplified the norovirus-specific nanobody sequenceusing error-prone PCR.The fragments were inserted into a yeast display vector, generating a nanobody library. Specifically, the error-prone PCR was carried out using a GeneMorph II Random Mutagenesis kit with an error rate of 6-9 nucleotide mutations per kilobase. The library was then transformed into Yeast EBY100 to display nanobody variants on the yeast surface. Next, we screened the nanobodies that can provide strong binding affinities to norovirus spike proteins by binding norovirus spike proteins and labeling them with fluorescence-tagged antibodies. After sequencing the screened nanobodies, we have identified the nanobody amino acid sequences.
Publications
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Xue Zhao, Mahbubur Rahman, Zhiyuan Xu, Tom Kasputis, Yawen He, Lijuan Yuan, R. Clay Wright, and Juhong Chen, Journal of Agricultural and Food Chemistry 2023 71 (22), 8665-8672
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Yawen He, Zhiyuan Xu, Tom Kasputis, Xue Zhao, Itati Iba�ez, Florencia Pavan, Marina Bok, Juan Pablo Malito, Viviana Parreno, Lijuan Yuan, R. Clay Wright, and Juhong Chen, ACS Applied Materials & Interfaces 2023 15 (31), 37184-37192
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