Progress 06/01/24 to 04/30/25
Outputs
Target Audience:Target audiences include stakeholders in forestry, agriculture, and pest control, and invertebrate biology, and educational opportunities in laboratory research for members of underrepresented groups. This has included recruitment and participation of (i) students at the high-school, undergraduate, post-baccalaureate, graduate, postdoctoral, and visiting scientist levels, (ii) scientists/academics in agriculture, forestry, mycology, entomology, pest control, and fungal genetics, development, and pathogenesis. Efforts have included: (i) educational: classroom and laboratory instruction and mentoring, participation and/or organizing informal educational programs, and participation in educational outreach programs/events, (ii) research: leading and instruction in experimental design including planning and execution, performing and mentoring students in data analyses and interpretation, participation in data dissemination, either directly or via instruction of laboratory personnel via attendance and presentation of posters, talks (including invited talks) at professional societies, organizing meetings, and writing and editing of manuscripts submitted to peer-reviewed journals. Target audiences have included stakeholders in forestry, agriculture, and pest control, and invertebrate biology, and educational opportunities in laboratory research. This has included recruitment(i) undergraduates, (ii)post-baccalaureate students, (ii) graduate students, and (iv)postdoctoral fellows Efforts have included research and education including leading and instruction in experimental design including planning and execution, performing and mentoring students in data analyses and interpretation, participation in data dissemination, either directly or via instruction of laboratory personnel via attendance and presentation of posters, talks (including invited talks) at professional societies, organizing meetings, and writing and editing of manuscripts submitted to peer-reviewed journals. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Throughout the project period, training and profession development has occurred on a range of different levels: Undergraduate students-the lab has hosted and mentored twoundergraduate students that worked on aspects of the project within the reporting period. Graduate Student-the lab has hosted one graduate student that assisted with insect rearing and data analyses. Post-doctoral Fellows-the lab has hosted two postdoctoral fellows: one with projects working in targeting the insect vector and the other on aspects of the H. lauricola-beetle interactions. How have the results been disseminated to communities of interest?Dissemination of results has mainly involved two activities, namely: (i) posters, presentations at relevant, and invited talks and lectures (of the PI and/or laboratory personnel), and (ii) via publication in peer-reviewed journals. Peer reviewed publications have included onepublicationgiven in the Journal of Fungi (IF = 4.7). What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
1. We have developed an image cell cytometry based method to examine fungal colonization of beetle mycangia and applied it to the study of H. lauricola-Xyleborus beetles. An manuscript has been submitted (Feb 2025) and is currently uder peer-review. Acknowledment of USDA funding is included in the paper. 2. We have completed our data collection with respect to comparative transcriptomic analyses of global gene expression in H. lauricola in host beetle mycangia.Data analyses are ongoing and will likely continue past the project end date, with the ultiamte goal of submission of the finding to a peer-reviewed journal.
Publications
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Joseph, R., Darrisaw, C., Lloyd, A., Hoel, D., and Keyhani, N.O. (2024) Isolation of a novel Pythium species, P. thermoculcivorax, and Trichoderma sp. from natural enzootic mosquito larval infections, Journal of Fungi, 10:199
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Progress 06/01/24 to 02/03/25
Outputs
Target Audience:Target audiences include stakeholders in forestry, agriculture, and pest control, and invertebrate biology, and educational opportunities in laboratory research for members of underrepresented groups. This has included recruitment and participation of (i) students at the high-school, undergraduate, post-baccalaureate, graduate, postdoctoral, and visiting scientist levels, (ii) scientists/academics in agriculture, forestry, mycology, entomology, pest control, and fungal genetics, development, and pathogenesis. Efforts have included: (i) educational: classroom and laboratory instruction and mentoring, participation and/or organizing informal educational programs, and participation in educational outreach programs/events, (ii) research: leading and instruction in experimental design including planning and execution, performing and mentoring students in data analyses and interpretation, participation in data dissemination, either directly or via instruction of laboratory personnel via attendance and presentation of posters, talks (including invited talks) at professional societies, organizing meetings, and writing and editing of manuscripts submitted to peer-reviewed journals. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Throughout the project period, training and profession development has occurred on a range of different levels: Undergraduate students-the lab has hosted and mentored two undergraduate students that worked on aspects of the project within the reporting period. Graduate Student-the lab has hosted one graduate student that assisted with insect rearing and data analyses. Post-doctoral Fellows-the lab has hosted two postdoctoral fellows: one with projects working in targeting the insect vector and the other on aspects of the H. lauricola-beetle interactions. How have the results been disseminated to communities of interest?Dissemination of results has mainly involved two activities, namely: (i) posters, presentations at relevant, and invited talks and lectures (of the PI and/or laboratory personnel), and (ii) via publication in peer-reviewed journals. Peer reviewed publications have included one publication given in the Journal of Fungi (IF = 4.7). Another publication is under review and one additional publication is anticiapted based on the data collected. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
We have developed an image cell cytometry-based method to examine fungal colonization of beetle mycangia and applied it to the study of H. lauricola-Xyleborus beetles. A manuscript has been submitted (Feb 2025) and is currently under peer- review. Acknowledgment of USDA funding is included in the paper. We have completed our data collection with respect to comparative transcriptomic analyses of global gene expression in H. lauricola in host beetle mycangia. Data analyses are ongoing and will likely continue past the project end date, with the ultimate goal of submission of the finding to a peer-reviewed journal.
Publications
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Joseph, R., Darrisaw, C., Lloyd, A., Hoel, D., and Keyhani, N.O. (2024) Isolation of a novel Pythium species, P. thermoculcivorax, and Trichoderma sp. from natural enzootic mosquito larval infections, Journal of Fungi, 10:199
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