Source: N Y AGRICULTURAL EXPT STATION submitted to NRP
MICROBIAL SAFE HARBOR AND SAFETY RECOMMENDATION FOR SMALL BEVERAGE PRODUCERS: SEAMOSS AS A MODEL SYSTEM
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1032305
Grant No.
2024-68008-42647
Cumulative Award Amt.
$300,000.00
Proposal No.
2023-09604
Multistate No.
(N/A)
Project Start Date
Jul 1, 2024
Project End Date
Jun 30, 2026
Grant Year
2024
Program Code
[A1701]- Critical Agricultural Research and Extension: CARE
Recipient Organization
N Y AGRICULTURAL EXPT STATION
(N/A)
GENEVA,NY 14456
Performing Department
(N/A)
Non Technical Summary
Seamoss is a spiky, sea vegetable that somewhat resembles frisée lettuce when it is in the ocean. When dried and packaged, the plant looks like cooked ramen noodles with tangled tendrils that clump together. Food manufacturers harvest it for its carrageenan, an ingredient that acts as a thickening agent for food like ice cream, chocolate milk and creamers. Seamoss refers to specific types of algae or seaweed that are naturally rich in certain nutrients, and minerals like folate, vitamin K, iodine, magnesium, calcium. While the plant can be consumed raw or supplement forms, it is mostly eaten in gel. The gel is made by soaking the dried plan in water, blending it, and letting it solidify in the fridge. Seamoss products are often sold by small manufacturers in the form of gels, or as an ingredient to a smoothie, or other juices. The producers have little to no food safety knowledge. Unfortunately, there are no foods safety guide for the producers wishing to include this ingredient in a food product specifically a beverage. In October 2022, The Michigan Department of Agriculture and Rural Development has recalled lemonade and gel products sold by Royal Seamoss. While no illnesses have been reported, the consumers were warned not to use or consume the product. The seamoss gel is area of concern, the pH is typically above 4.60 unless acidified. Depending on the viscosity, this product might have anaerobic pocket if not packaged correctly. Thus, increasing the risk for C. botulinum.In 2011, the FDA Food Safety Modernization Act (FSMA) was signed into law to expand the regulatory authority of the FDA, allowing them to increase their focus on foodborne illness prevention and not just outbreak response. The edible seaweed has yet to be regulated although the consumption is project to increase. Therefore, the proposed project plan to analyze commercially available seamoss and seamoss gels for pathogens and heavy metals. Afterwards, we will test the three most popular seamoss recipes to see if they support the growth of pathogen during storage at room temperature and under refrigerated conditions. Finally, we will create guidance documents for the industry and disseminate the results on our website, webinar, conferences, and scientific publications.
Animal Health Component
100%
Research Effort Categories
Basic
0%
Applied
100%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
5025010110030%
9035010110070%
Goals / Objectives
Seamoss, also known as Irish moss or Chondrus Crispus, is used as often a thickening agent. It is an edible sea vegetable in the algae family that is packed with nutrients like folate, vitamin K, B, iron, iodine, magnesium, zinc, and calcium. In the past three years, seamoss specifically has become very popular in US. Major news providers like The New York Times, CNBS, Forbes, Wall Street Journal, CBS news, the Seattle Times and many more have released articles highlighting seamoss potential health benefits. Celebrities like the Kardashian, and social media influencers are raving about the benefits and encouraging its consumption. During COVID, there has been an increase of seamoss products sold in the US market because it was believed to fight against the pandemic. Unfortunately, there are no foods safety guide for the producers wishing to include this ingredient in a food product specifically a beverage. Therefore, we proposed to:Objective 1: Survey commercially available seamoss products for physiochemical properties, microbial quality and heavy metals.Objective 2: Evaluate pathogens growth based on seamoss gel formulations and producers handling procedures.Objective 3: Identify critical control points to produce safe seamoss beverages and develop training and extension material for producers
Project Methods
Objective 1: Dried seamoss samples and seamoss gels will be purchased and collected, on a nation-wide basis from retail specialty show, farmers market, and online sources. Once samples are received, the seamoss type, the harvesting locations, purchase location will be recorded and coded accordingly. The water activity (Aw), the pH, Brix, Dissolved Oxygen (DO) will be measured using a water activity meters, a pH meter, refractometer, and a DO sensor specifically. The Aw will be tested for dried seamoss. And the pH, Brix, and DO will be measured for the seamoss gels. Dried seamoss and seamoss gels samples will be analyzed for aerobic plate count, yeast and mold, coliform, E. coli O157:H7, Staphylococcus aureus, Bacillus cereus, Vibrio spp., Listeria monocytogenes, and Salmonella spp. according to Bacteriological Analytical Manual. All experiments will be performed in triplicate. The data will be analyzed using descriptive statistics, graphs, and tables. And all statistical analysis will be performed using R or a similar softwareObjective 2: Isolated colonies from Objective 1 (E. coli, Salmonella spp., L. monocytogenes, B. cereus, Vibrio spp., Aeromonas spp.) will be grown overnight in Tryptic Soy Broth overnight at 35°C. Culture will be diluted to spot inoculated the dried seamoss. The inoculum will be 10 ml or less to not modify the water activity of the original product. The dried inoculated seamoss (10 g) will be soaked in sterile water (90 g) and stored at refrigerated and room temperatures (~4 and 20°C). The soaking times will vary from 0, 4, 6, 12, and 24 hours. An additional variable is that we also soak the seamoss for 24 hours with 1 and 5% lemon juice and study the pathogens survival. At the end of the soaking time, the microbial population will be quantified in the seamoss and the water. Samples will be serially diluted in the appropriate buffer, homogenized, and plated on the selective media accordingly. Experiments will be done in triplicate. We will use three of the most used seamoss gel recipe that we collected and summarized. This a standard shelf life study and pathogens will not be used here. We will prepare the seamoss gel as suggested and perform a shelf life study on it. The gels will be stored under refrigeration and at room temperature. We will test on days 0, 7, 14, 21, 28, 35 when refrigerated. When stored at room temperature, we will test on days 0, 3, 6, 9. The study will be terminated if there are visible signs of spoilage, or the microbial count is over 6 log CFU for total plate count or yeast/mold count.Objective 3: Based on results from Obj. 1 and 2, we will characterize the specific hazards associated with seamoss. We will make recommendations to mitigate the food safety risk during processing and handling. CCPs will be determined for each specific processing and handling step of producing seamoss beverages. Once completed, beverage producers can incorporate CCPs in their HACCP plan. We will focus on developing guide "Food Safety Recommendations for Seamoss Beverage Producers" that will include training and extension materials for three the targeted groups: (i) the small beverages producers, (ii) the regulators, and (iii) the extension personnel and educators. This proposed guide will be tool to support the seamoss industry. It will review the critical control points and measures recommended to produce safe seamoss beverages. The educational resources will be developed based on current knowledge and new knowledge generated by the proposed.

Progress 07/01/24 to 06/30/25

Outputs
Target Audience:To date, engagement with relevant stakeholders has not yet occurred. However, there is a planned for the upcoming fall season. Changes/Problems:When the project was initially designed, we assumed that acquiring sea moss products would be a straightforward process. Our original goal was to purchase between 80 and 120 unique products. Despite our efforts, many manufacturers were unable to fulfill our orders--an unexpected challenge, particularly given that we sourced from major online retailers such as Amazon and Etsy. Ultimately, we were able to obtain approximately 63 products. While this number was lower than anticipated, we decided to proceed in order to avoid further delays in the project timeline. An ongoing challenge with this project has been navigating recent changes in federal funding priorities. As a result, our institution implemented a temporary hiring pause, which unfortunately led to the cancellation of the summer scholar program and impacted our original staffing plans. What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?Right now (summer 2025), we are conducting a shelf life study where we are making seamoss gel under different conditions. We will prepare the seamoss gels plain, with lemon/lime juice, pasteurized, with apple juice and stored under at room temperature or a refrigerated temperature. This is on-going. The results on of this study will provide important knowledge so that extension professional can provide the food industry. In October 2025, we will host a webinar series focused on food safety recommendations for small-scale producers of sea moss products. The aim is to engage with businesses involved in the processing of sea moss. The webinar topics will include: What is Sea Moss? Understanding the Hype Food Safety Considerations in Sea Moss Gel Production To Cook or Not to Cook? Process Controls for Sea Moss Gel Preparation In addition to the webinars, we will develop accompanying infographics to reinforce key messages. Recognizing that individuals have different learning preferences, we aim to provide multiple formats to support broader understanding and accessibility. Simultaneously, we will continue work on Objective 2, which focuses on evaluating pathogen growth in sea moss gel formulations. This step is critical because the pathogens have been isoalted from the raw materials. This is exciting. We also plan to advance manuscript preparation, with the goal of submitting our findings to peer-reviewed journals by the end of the year.

Impacts
What was accomplished under these goals? Objective 1 "Survey commercially available seamoss products for physiochemical properties, microbial quality and heavy metals" of this project is completed. Seamoss products were purchased from an online global marketplace (Etsy and Amazon) to ensure an unbiased selection of commercially available samples. A total of 53 dried seamoss and 13 seamoss gel samples were obtained from online sources. The samples that were delivered varied in colors and consistency across sellers. Samples were also categorized by color and country of origin. It is important to note that efforts were made to purchase more samples, however the requests were not fulfilled. The seamoss products were analyzed for physiochemical properties like water activity, pH, Brix and for microbiological analyses including total plate cuont, yeast and mold count. The products were also screened for pathogens including Salmonella, E. coli, Staphyloccocus, Bacillus and Vibrio. The products were also sent to an outside lab and evaluated for heavy metals analyis. This study is the first to report on the microbial quality and heavy metal content of commercially available dried seamoss and seamoss gel products. The findings revealed considerable variability among products in terms of geographic origin, color, quality, and physicochemical properties. As expected, total aerobic plate counts were high in dried sea moss samples, likely due to the raw and unprocessed nature of the product. Microbiological analysis indicated the presence of Staphylococcus, Bacillus, Vibrio, and Escherichia species across many samples. While the detection of these genera does not confirm the presence of specific pathogens, it raises concerns about potential microbial contamination and highlights the need for further confirmatory testing. Notably, seamoss gels despite being processed also exhibited high total plate counts as well as elevated yeast and mold counts, suggesting significant variation in formulation and a lack of standardization. In addition to microbiological concerns, several the dried seamoss were found to contain detectable levels of heavy metals, including mercury, arsenic, and cobalt. Although no regulatory limits currently exist for heavy metal concentrations in seaweed products in the United States or the European Union, the chronic consumption of contaminated products may pose health risks over time. Overall, this study underscores the urgent need for more comprehensive research to assess the food safety risks associated with sea moss consumption. Establishing clear regulatory guidelines and product standards will be essential as the market continues to expand. The data generated through this study will serve as a critical foundation for informing the technical guidance provided to food producers, particularly those involved in the seamoss industry. Seamoss products are predominantly produced and marketed by small-scale entrepreneurs who may have limited access to food safety education, infrastructure, or regulatory knowledge. Historically, such producers are more vulnerable to gaps in food safety practices, which can pose risks to public health. By characterizing the microbiological and chemical safety of commercial seamoss products, this study provides baseline data that will support the development of targeted extension materials, technical trainings, and educational resources. These tools will help raise awareness, build capacity, and promote safer production and handling practices within this rapidly growing, yet underregulated, sector of the food industry.

Publications

  • Type: Theses/Dissertations Status: Submitted Year Published: 2025 Citation: MICROBIAL AND HEAVY METAL ANALYSES OF COMMERCIALLY AVAILABLE SEA MOSS AND SEA MOSS PRODUCTS