Source: DONALD DANFORTH PLANT SCIENCE CENTER submitted to NRP
ENHANCING COVER CROP FOUNDATIONAL KNOWLEDGE FOR SOIL DYNAMICS AND CORN PRODUCTION THROUGH ADVANCED ROOT PHENOMICS, SPECIES DIVERSITY, AND AGROECOSYSTEM MODELING
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1032184
Grant No.
2024-67013-42380
Cumulative Award Amt.
$650,000.00
Proposal No.
2023-09664
Multistate No.
(N/A)
Project Start Date
Jul 1, 2024
Project End Date
Jun 30, 2027
Grant Year
2024
Program Code
[A1102]- Foundational Knowledge of Agricultural Production Systems
Recipient Organization
DONALD DANFORTH PLANT SCIENCE CENTER
975 NORTH WARSON ROAD
ST. LOUIS,MO 63132
Performing Department
(N/A)
Non Technical Summary
Cover cropping has been largely considered a major conservation approach to improve ecosystem services for sustainable agriculture. With current adoption rates low across US farmlands, extensive investments from government and private sectors have strongly encouraged farmers to employ cover crops. However, the rapid momentum from these investments is not well supported by a sufficient foundational knowledge base of cover cropping such that we are ready to scale up its adoption with maximum effectiveness. A major barrier is the limited understanding of cover crop root system traits and their empirical effects on soil and cash crops, especially across the spectrum of cover crop species diversity. In this project we will conduct multi-year trials of twelvecover crop species that integrate with corn production, and use root phenomics, cutting-edge sensing technologies, and machine-learning enabled agroecosystem modeling to gain an improved understanding of the variation for root traits that exists among diverse cover crop species and their influence on soiland cash crops. The societal benefit of this study will be to utilize our enhanced understanding of these relationships to provide more informed species selection that maximizes both yield and ecosystem benefits and thereby supports widespread adoption of cover crop management practices in the US.
Animal Health Component
(N/A)
Research Effort Categories
Basic
70%
Applied
(N/A)
Developmental
30%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1021510106025%
1022140106025%
2051510107025%
2052140107025%
Goals / Objectives
The major goal of the project is to fill key knowledge gaps in the foundational knowledge base of cover crop plant species that currently hinder their efficacy and farmer adoption. First, there is a limited understanding of cover crop root system phenotypes and their empirical effects on soils and cash crops. Second, the species diversity of cover crop above- and belowground traits are largely underexplored. Three main objectives support this effort:OBJ 1: Characterize aboveground and belowground phenotypic variation across diverse cover crop species.OBJ 2: Empirically assess the impacts of cover crop root phenotypes on soil properties and corn production.OBJ 3: Improve agroecosystem modeling for simulating cover crop traits and their impacts on soil dynamics and corn production, with implications to prototype decision-support tools for cover crop selection and management.
Project Methods
The project will be conducted at the Danforth Center's field research site across three years. Standard approaches to collect cover crop shoot (cross-sectional grids and hand harvesting) and root (1-2m soil cores) samples will be employed. Both tissues will be ground and analyzed for total nitrogen and carbon content to estimate N:C ratios above and belowground, and to estimate contributions of total N and C to the plot and subsequent corn crop. Statistically significant differences in shoot and root phenotypes among functional groups and species will contribute to a foundational knowledge base for rational design of improved cover crops. To measure corn root growth dynamics we are employing a novel root growth sensing tool, RootTrackers, as well as the "Root Front Velocity" methods employed thus far in just a few published studies. Corn nitrogen and yield will be measured with conventional means. Differences in root growth, nitrogen uptake, and yield will be assessed as an outcome of the diverse cover crops they were paired with. All of these parameters will be assessed over time as the same species will be planted in the same plots year-on-year. Remote hyperspectral imaging will be conducted by manned aircraft and paired with the aforementioned measurements to develop ground truth relationships that allow modeling over a large scale of cover crop and corn growth and nitrogen capture. Model prediction efficacy (ex. total plant nitrogen, total biomass) will be evaluated with manual ground truthing methods. The measurements will also be used to parameterize the ecosys model of diverse cover crop species to enable simulations of the effects of management on corn production, and to prototype a decision support tool that producers can use to maximize both profit and ecosystem service benefits.

Progress 07/01/24 to 06/30/25

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project has provided numerous opportunities for the professional, technical, and scientific development of students, technicians, and early-career scientists. Training has been facilitated through hands-on experience in leading and participating in field sampling campaigns, laboratory processing, as well as building expertise in established scientific protocols. For example, one technician independently developed specialized expertise in installing, maintaining, and interpreting data from LICOR gas flux chambers, becoming the Topp lab's lead resource in this area. Professional growth has also been supported by opportunities to prepare and present research findings at national scientific conferences, further advancing career development across multiple stages. Professional development has also been supported through dissemination of project findings. To date, data generated by this project has contributed to four independent conference abstracts led by a senior scientist, postdoctoral researcher, graduate student, and technician. These were submitted to the CANVAS Tri-Societies Conference (ASA, CSSA, SSSA) and the American Geophysical Union Annual Meeting (AGU25), where results will be presented in 2025. Collectively, these opportunities reflect the project's commitment to training the next generation of agricultural scientists and supporting career advancement at multiple levels. How have the results been disseminated to communities of interest?Project findings have been actively prepared for dissemination to scientific communities. To date, data generated through this research has contributed to four independent conference abstracts led by a senior scientist, postdoctoral researcher, graduate student, and technician. These were submitted to the CANVAS Tri-Societies Conference (ASA, CSSA, SSSA) and the American Geophysical Union Annual Meeting (AGU25), where results will be presented in 2025. By engaging multiple team members in presenting research outcomes, the project ensures broad dissemination of findings to both agricultural and interdisciplinary audiences while amplifying visibility of the work across professional networks. Conference abstracts: Zhao Jiang, Kaiyu Guan, Sheng Wang, Kong Wong, Kirsten Hein, G. Cody Bagnall, Christopher N. Topp. Monitoring Phenotypic Variation Across Diverse Cover Crop Species and Assessing Their Impacts on Subsequent Corn Production via Airborne-Satellite Cross-Scale Sensing. AGU25 Hein, K., Bagnall, G. C., Bauer, M., Griffiths, M. D., Wong, K., & Topp, C. (2025) Exploring Shoot and Root Trait Diversity across Functional Groups of Cover Crops. CANVAS 2025, Salt Lake City, UT. G. Cody Bagnall, Shayla Gunn, Mao Li, Ajay Kumar Patel, Alifu Haireti, Vasit Sagan and Christopher Topp. Advancing Root System Research: Species-Level Classification Using Hyperspectral Imaging. CANVAS 2025 G. Cody Bagnall, Bartolo Giuseppe Dimattia, Matthew Bauer, Kirsten Hein, Kong Wong, Marcus D. Griffiths, and Christopher Topp. Belowground Impacts of Cover Crops: Linking Root Traits, Soil Water Retention, and Gas Fluxes. CANVAS 2025 What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period, we will complete fall 2025 field sampling and finalize data processing for year three cover crop, soil, and corn measurements. With a comprehensive multi-year dataset in place, we will conduct integrated analyses to evaluate the impacts of diverse cover crop traits on soil properties and subsequent corn production. Building on these analyses, we will initiate manuscript preparation for submission to high-impact scientific journals. Planned publications include: (i) a multi-year experimental resource describing above- and belowground phenotypic variation in diverse cover crops, (ii) a synthesis of measurements linking cover crop traits to corn production outcomes, and (iii) additional manuscripts depending on data quality, processing timelines, and new hypotheses generated from results and relevant literature. Collectively, these activities will translate the project's datasets into scientific outputs that advance understanding of cover crop contributions to sustainable agricultural systems

Impacts
What was accomplished under these goals? We have advanced a 13-acre longitudinal field study at the Donald Danforth Plant Science Center Research Site to evaluate the role of diverse cover crop species in no-till systems, with additional plots maintained under fallow and conventional tillage treatments. Objective 1: Characterize aboveground and belowground phenotypic variation across diverse cover crop species. Comprehensive phenotypic datasets have been collected and processed, with analyses underway. These include three-years of shoot biomass data and stand counts, two-years of root biomass and leaf C and N concentration measurements, and a full analysis of cover crop root system architecture from the first year. Ongoing work in 2025 is expanding this dataset through flatbed scanning of excavated roots and minirhizotron imaging, which will provide a second year of root system architecture and a more detailed characterization of belowground traits and their change over time. During the 2024-2025 season, airborne hyperspectral images have been collected for the bare soil before cover crop planting, diverse cover crops in the spring, and also corn after the cover crop termination. The hyperspectral images with 0.5-meter resolution and spectral coverage of 400-2500 nm will enable high-throughput phenotyping of diverse cover crop species and corn. The airborne hyperspectral-based crop trait maps will support the upscaling to daily 3-meter resolution PlanetScope satellite data for the continuous monitoring of cover crop traits and corn productivity. Objective 2: Empirically assess the impacts of cover crop root phenotypes on soil properties and corn production. Data collection and processing for soil and corn responses to cover crop adoption have also progressed. To date, analysis-ready datasets include two years of plot-level measurements of soil pH, organic matter, nitrate, ammonium, and C:N ratios, along with one year each of aggregate stability, bulk density, and hydraulic conductivity. Additional completed datasets include two years of cover crop residue biomass and two years of corn yield and biomass. In 2025, this effort was expanded in the spring to include pre-termination measurements of soil potentially mineralizable carbon, soil C and N pools, and continuous CO? and N?O fluxes in cereal rye and winter barley plots using LICOR long-term and survey chambers. Following cover crop termination, summer measurements focused on continued gas flux monitoring and corn leaf N content. Final sampling planned for Fall 2025 will include soil texture, bulk density, aggregate stability, and end-of-season corn yield, completing the third year of this multi-year dataset. Together, these accomplishments establish a robust foundation of multi-year, multi-trait datasets that will enable integrated analyses of how cover crop traits influence soil health and corn productivity. This work is advancing knowledge of plant-soil interactions in agroecosystems and will provide critical insights for designing resilient, productive cropping systems

Publications