Progress 06/01/24 to 05/31/25
Outputs
Target Audience:The unique anthocyanin-enriched bioengineered tomato varieties developed for farmers, the new value-added healthy products for consumers, and the advanced 3D cell culture and animal model for scientific community. This proposal will not only develop the unique anthocyanin-enriched bioengineered tomatoes but also evaluate their impact on colonic cancer prevention and underlying mechanisms in both in vitro and in vivo models. Furthermore, graduate students and postdoctoral fellows will be trained through this project. The results will be presented in national or international conferences and published in peer-reviewed scientific journals. Changes/Problems:One of the co-Project Directors, Dr. Xiuzhi Susan Sun, has retired from Kansas State University. As a result, her role as co-Project Director for this project has been transferred to Dr. Donghai Wang. This change, outlined in the letter dated February 27, 2025, has been reviewed and approved by the office and the NIFA cognizant program official, Mark Heap, on March 4, 2025. What opportunities for training and professional development has the project provided?Several graduate and undergraduate students, along with one research associate, participated in this project and received training in various areas, including novel transgenic tomato line development, anthocyanin extraction and analysis, cell culture treatments, and manuscript development and publication. How have the results been disseminated to communities of interest?The results of this research are primarily disseminated through peer-reviewed journal publications and national or international conference presentations. To date, one peer-reviewed article has been published in Foods. What do you plan to do during the next reporting period to accomplish the goals?The studies planned for the upcoming year will focus on addressing the remaining aspects of Objective 1. Specifically, we will continue investigating the anti-cancer mechanisms of anthocyanin-enriched transgenic tomato extracts in both normal colonic epithelial cells and colonic adenocarcinoma cells using both traditional 2D and advanced 3D cell culture systems. Our primary approach will be to examine the suppression of inflammatory cytokine-induced mitogenic signaling in vitro. All proposed studies will proceed as outlined in the original research plan.
Impacts
What was accomplished under these goals?
Supported by this five-year USDA NIFA research project, several tasks from Objective 1 are being investigated in parallel for this first-year report. We have developed novel transgenic tomato lines and analyzed their anthocyanin profiles and content using HPLC. These lines are now ready for use in cell culture studies and upcoming animal feed experiments. To initiate our research, we conducted a comprehensive review of recent literature and published a review paper summarizing our progress and outlining our research plan for the next one to five years. Concurrently, we have continued exploring the anti-inflammatory effects of anthocyanin-enriched transgenic tomato extracts and their potential metabolites in both normal colonic epithelial FHC cells and adenocarcinoma SW480 cells. The potential mechanisms by which these extracts and/or their metabolites contribute to cancer cell inhibition will be further assessed in next year's 3D cell culture studies. All research activities have been conducted in full alignment with the original proposal, without modification.
Publications
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Md Suzauddula, Kaori Kobayashi, Sunghun Park, Xiuzhi Susan Sun, Weiqun Wang. Bioengineered Anthocyanin-enriched tomatoes: a novel approach to colorectal cancer prevention. Foods 2024; 13(18), 2991. https://doi.org/10.3390/foods13182991
|