Source: LAC COURTE OREILLES OJIBWA COMMUNITY COLLEGE submitted to NRP
HEMP INTERCROPPING TO BUILD SOIL HEALTH AND PROFITABILITY
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1031691
Grant No.
2024-38424-41473
Cumulative Award Amt.
$650,000.00
Proposal No.
2023-06907
Multistate No.
(N/A)
Project Start Date
Jan 1, 2024
Project End Date
Dec 31, 2027
Grant Year
2024
Program Code
[ZY]- Tribal Colleges Research Grants Program
Recipient Organization
LAC COURTE OREILLES OJIBWA COMMUNITY COLLEGE
13466 WEST TREPANIA ROAD
HAYWARD,WI 54843
Performing Department
(N/A)
Non Technical Summary
Developa project evaluation planthat utilizes the market fluctuation analyzes, hemp regulations,yield or pest deterrent benefits offered by companion plantsthatwill hold true regardless of hempprices,collectdata on cultivar performance in extremeweather events to define whichcultivars arewell adaptedto our evolving regionalclimate and utilize the trials to develop replicated processes to ensure data collection is able to continue for sustainablity and results.Gain knowledge and understanding on the design and layout of field plots through applying alternative cultivation methods.Harvest under intercropping production systems while understanding the close relationship of crops with theirecosystems.Utilizing the process creat a training process for faculty and student intern's in the processing and analyzing flower samples for cannabinoid content.This training will be utilzed byLCOOU faculty and students in data collection and cannabinoidsampling methods that can be conducted at the LCOOU facilities. The findgings will be shared out in various methods of media, workshops and conferences,
Animal Health Component
50%
Research Effort Categories
Basic
30%
Applied
50%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1321730114025%
1021730101025%
2021730108050%
Goals / Objectives
GoalsDevelop a sustainable cropping system for hemp grown at LCO.Build seed sovereignty for the tribe.Increase community awareness of alternative cropping systems and crops.Objectives:Evaluate the effectiveness of intercropping to enhance soil health, hemp productivity and farm profitability.Develop a local adapted dual purpose help cultivator for LCO.Promote knowledge sharing and adoption of hemp intercropping among tribal members, farmers, and agricultural stakeholders.?
Project Methods
Data Analysis and InterpretationCrop trait data will be collected in 1,2,3 and analyzed with parametric statistics to look at differences of the intercropping treatmentsData will be analyzed and summarized utilizing R scripts and trial reports and manuscripts will be generated for wide distribution.Using one way ANOVA with companion crops test the hypothesis that hemp microbiome diversity and taxon abundances will differ between treatments.Research Capacity EnhancementUW personnel will participate in layout and initial implementation of experimental plots at LCOAssist in initial data acquisitions and analyses.Work side by side with the LCOOU staff and student internsYear 1-4 field research tours will provide and open to the community to learn about the research project and preliminary results.Soil Sampling: At planting and harvesting of each year, soil assessments will be performed on all experimental plots from composite soil core samples.Bulk soil will be sampled from the plant neighborhood by removing and homogenizing 10 cores per plot.Samples will be stored on ice and returned to campus. Samples will be sleeved through 2mm meshA portion will either be air dried for soil chemical analysis or stored at -80 C for subsequent analyses and archiving.Soil health assessments will be conducted for soil texture, PH, Nutrients, C and N content of SOM with relatively fast turn over (particulate organic matter) and relatively slow turnover (mineral-associated organic matter)Five weeks after floral initiation in hemp, biogeochemical analyses will also be performed and will include measurement of soil respiration, and net N mineralization.All soil characterization will be conducted at the UW Madison Soil and Forage Analysis Lab in Marshfield WIMicrobial Community AssessmentSoil microbial community composition will be assessed using methods consistent with the Earth Microbiome Project standard protocol.Total DNA will be extracted from bulk soil samples using the DNeasy Powersoil Pro DNA Insolation KitThe bacterial 16SrRNA gene and fungal internal transcribed spacer (ITS2) region will be amplified, quantified, and pooled together in equimolar concentrations for sequencing on an Illumina MiSeq machine at the UW Madison Biotechnology center using 2x300 bp chemistry.Paired end sequences will be merged de-multiplexed strangely quality filtered and amplicon sequence variants will be picked using the DADA2 pipeline in QIIME2.Taxonomy will be assigned to ASVs using the RDP classifier against most recent versions of the SILVA or UNITE databases for 16SrRNAand ITS2 gene sequences respectively.Plant healthData will be collected utilizing the field book application to standardize observations, reduce error and increase efficiency.Plant height will be recorded on a bi-weekly basis.Feminized seed will be utilized for trial but will scout and record the presence of male parts.Data will be collected for flowering time, maturity, and insect/disease pressure.Data collected for the companion crops will include yield and economic factors (seed cost, labor, inputs, specialized equipment, sale price)Floral plant tissue will be collected every 4 weeks into flowering and at harvest in a similar fashion to the federally mandated protocol to assess cannabinoid contentCannabinoid content will be calculated as total CBD=CBD+(CBDAx0.877) and total THC=(THCAx0.877) in accordance with the USDA hemp Final Rule.mixture of 10 feral hemp populations collected will be planted in the UW-Mad. Walnut Street Greenhouse in a 500 2gallon pots with 2 seeds each and allowed to cross pollinate.Bulk seed will be collected and sorted by sizeThe largest seeds will be planted at LCO in a 20x20 ft plot the following year for evaluation.Mass selection utilizing a selection index for flowering time plant height seed size stem diameter and seed yield will be used to select the top 5% of plants.The same selection index and pressure will be utilized in year 2 and 3In year 4 seed from each selection will be grown in a RCD to calculate genetic gain from selection.

Progress 01/01/24 to 12/31/24

Outputs
Target Audience:Hemp,Cannabis sativaL.,was legalized in the 2018 Farm Bill forindustrial production.Growing boomed, butprofitable agronomic practices still lag in the infant industry.Growers arespecificallyconcerned about weed and pest managementstrategiesthat do notimpactyield or quality--as there are no certified herbicides or pesticides for hemp--and theywant agronomic practices to be sustainable or even regenerative, bettering the land than when they started farming.Our researchprojected was created to assess the utility of companion cropping to address these questions. Companion cropping is a sustainable and cultural management tactic within farming systems in which a secondary crop is planted (in some spatial or temporal configuration) with the main crop, offeringan array ofpotential benefits and ecosystem services. Some of the benefits of companion crops include weed control, increased pollination and habitats for beneficial insects, pest deterrence, increased crop productivity, and soil resilience. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This work was presented at several field days including the 2024Wisconsin Department of Agriculture and Consumer Protection Field day, 2024Arlington ARS HempField day on 20AUG24, and the 2024Spooner ARSHemp Field day on 26SEP24. Findings were disseminated at two field days attended by farmers and agricultural stakeholders. Informational brochures and research summaries were distributed during the events. Data collected is being compiled for future publications and presentation at agricultural conferences. A graduate student, a research technician and two undergraduate students gained hands-on experience in hemp intercropping, data collection, and soil microbiome sampling. They also expanded skillsets in cannabinoid sampling, soil analysis, and greenhouse seed production. Also by presenting research findings at field days, they enhanced their public speaking and science communication skills Two undergraduate students assisted with collecting and analyzing data for this project How have the results been disseminated to communities of interest?This work was presented at several field days including the 2024Wisconsin Department of Agriculture and Consumer Protection Field day, 2024Arlington ARS HempField day on 20AUG24, and the 2024Spooner ARSHemp Field day on 26SEP24. Findings were disseminated at two field days attended by farmers and agricultural stakeholders. Informational brochures and research summaries were distributed during the events. What do you plan to do during the next reporting period to accomplish the goals?Repeat intercropping experiments at Arlington and Spooner in the 2025 growing season. Grow selections at LCO farm to develop the dual-purpose hemp cultivar. Organize additional field days to share updated results. Begin analysis of 2024 soil and plant data to identify trends in soil health and plant yield. Expand outreach efforts by presenting findings at local tribal and regional agricultural meetings

Impacts
What was accomplished under these goals? Todeterminewhich companion crops are most suitable within high cannabinoid hemp production, we analyzedthe effects ofintercroppingfive companions (basil, dill, cilantro, sage, and marigold) on:i) companion plant yield, ii) weed competition, iii) insect diversity, iv) hempbiomassyield, and v)cannabinoid content. This experiment was conducted at threelocations in 2024(LCO, Arlington ARS and Spooner ARS). Results show that companion cropsdifferentially andsignificantlyimpactweed coverand insect diversity, but do not significantlyimpactyield or cannabinoid content. This means that growerscan choose companion crops that fit their farm and equipment best without having to worry about a negative impact to quality and yield. Future studies will focus on implementation of companion cropping through on farm trials,an exciting and necessary next step to a sustainable future for cannabis production. Weed cover ranged from 0 to 85 percent between companions across all locations. A significant difference was found between companions and their ability to suppress weeds, (p < 0.001). The marigold treatment decreased weed cover when compared to all other treatments (p<0.05 for marigold compared to all treatments). Basil also significantly decreased weed cover (p= 0.032) when compared to blank treatments, appearing to have a suppressive effect on weeds across all environments. All other treatments -dill, blank, cilantro, and sage -did not statistically differ from each other in their ability to suppress weeds. Plant height ranged from 87.2 cm to 175.8 cm between companions across environments. No significant difference was found between companions and hemp plant height (p=0.26). Dry weight ranged from 0.2 to 2.56 pounds across companions and environments. Companion treatments did not statistically impact the dry weight of hemp plants, but there was a difference between environments for dry weight (p<0.001). Bucked weight ranged from 0.015 to 1.35 pounds across environments and companions. There was no statistical difference between companion treatments and bucked weight of hemp. Companion harvest potential value ranged from 0 to 798 dollars between companion treatments across all environments. A significant difference was found between companions and their possible value, (p<0.001). The basil treatment had significantly more potential value than any other treatment (p<0.001). Cilantro, dill, and marigold had significantly more possible worth than blank and sage, which were significantly similar and had the least potential value. THC ranged from 0.07 to 0.525 percent between locations and treatments; CBD ranged from 2.91 to 14.76 percent between locations and treatments. Both THC and CBD were not statistically impacted by companion treatments. There were more total insects in timepoint one than timepoint two across all classifications (pest, beneficial, incidental). At the first timepoint sampling, significant differences were found for the number of pests between treatments specifically between marigold and dill, with marigold having statistically less pests present that dill in all three environments. No statistical differences were observed for the other categories in timepoint one, meaning that there were no differences between numbers of beneficial or incidental insects between companion crops. Insect results from timepoint two highlight a significant difference between companions (p=0.041) specifically between basil and cilantro treatments, with cilantro having more beneficial insects than basil. • Six populations of feral hemp were planted at LCO University Farm and allowed to intercross. • Top 10% of plants (based on height and flowering time) were selected for seed harvest. • Seeds were size-selected, and 400 plants are currently growing in the UW Madison Walnut Street greenhouse for seed production for the 2025 season. Future studies should focus on expansion of this project through on farm trials with larger plot sizes, as well as long term soil assessments, in order to better understand how companion cropping in cannabis affects ecosystem services long term.

Publications


    Progress 01/01/24 to 02/06/24

    Outputs
    Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

    Impacts
    What was accomplished under these goals? odeterminewhich companion crops are most suitable within high cannabinoid hemp production, we analyzedthe effects ofintercroppingfive companions (basil, dill, cilantro, sage, and marigold) on:i) companion plant yield, ii) weed competition, iii) insect diversity, iv) hempbiomassyield, and v)cannabinoid content. This experiment was conducted at two locations in 2021 (LCO and WMARS) and one location in 2022 (WMARS). Results show that companion cropsdifferentially andsignificantlyimpactweed coverand insect diversity, but do not significantlyimpactyield or cannabinoid content. This means that growerscan choose companion crops that fit their farm and equipment best without having to worry about a negative impact to quality and yield. Future studies will focus on implementation of companion cropping through on farm trials,an exciting and necessary next step to a sustainable future for cannabis production. Weed cover ranged from 0 to 85 percent between companions across all locations. A significant difference was found between companions and their ability to suppress weeds, (p < 0.001). The marigold treatment decreased weed cover when compared to all other treatments (p<0.05 for marigold compared to all treatments). Basil also significantly decreased weed cover (p= 0.032) when compared to blank treatments, appearing to have a suppressive effect on weeds across all environments. All other treatments -dill, blank, cilantro, and sage -did not statistically differ from each other in their ability to suppress weeds. Plant height ranged from 87.2 cm to 175.8 cm between companions across environments. No significant difference was found between companions and hemp plant height (p=0.26). Dry weight ranged from 0.2 to 2.56 pounds across companions and environments. Companion treatments did not statistically impact the dry weight of hemp plants, but there was a difference between environments for dry weight (p<0.001). Bucked weight ranged from 0.015 to 1.35 pounds across environments and companions. There was no statistical difference between companion treatments and bucked weight of hemp. Companion harvest potential value ranged from 0 to 798 dollars between companion treatments across all environments. A significant difference was found between companions and their possible value, (p<0.001). The basil treatment had significantly more potential value than any other treatment (p<0.001). Cilantro, dill, and marigold had significantly more possible worth than blank and sage, which were significantly similar and had the least potential value. THC ranged from 0.07 to 0.525 percent between locations and treatments; CBD ranged from 2.91 to 14.76 percent between locations and treatments. Both THC and CBD were not statistically impacted by companion treatments. There were more total insects in timepoint one than timepoint two across all classifications (pest, beneficial, incidental). At the first timepoint sampling, significant differences were found for the number of pests between treatments specifically between marigold and dill, with marigold having statistically less pests present that dill in all three environments. No statistical differences were observed for the other categories in timepoint one, meaning that there were no differences between numbers of beneficial or incidental insects between companion crops. Insect results from timepoint two highlight a significant difference between companions (p=0.041) specifically between basil and cilantro treatments, with cilantro having more beneficial insects than basil. Future studies should focus on expansion of this project through on farm trials with larger plot sizes, as well as long term soil assessments, in order to better understand how companion cropping in cannabis affects ecosystem services long term.

    Publications