Progress 06/01/24 to 05/31/25
Outputs
Target Audience:The target audience for this reporting period includes graduate, undergraduate, and high school students; community college students in the UH System; federal and state agencies; USDA APHIS; Hawaii Department of Agriculture; Hawaii Department of Land and Natural Resources; Hawaii Invasive Species Council; Coordinating Group on Alien Pest Species; Western Plant Board, University of Guam and other universities on the mainland; participants of the American Phytopathological Society's Annual Meeting 2024; Participants of other national and international meetings where we presented; and NextGen PaCoN seminar participants from various universities and institutes. Changes/Problems:Grant suspension in January 2025: A major setback to project progress The suspension of the NextGen PaCoN grant in January 2025 has significantly affected the project, resulting in the cancellation of many planned activities for Spring and Summer 2025. The continued uncertainty regarding the grant's reinstatement poses further challenges for Fall 2025 and Spring 2026 programming. This disruption has impacted several core components of the program, including student recruitment, internships, travel, and training opportunities. Since Spring 2025, we have been unable to recruit new graduate students or promote upcoming academic opportunities. Additionally, all domestic and international travel--including planned internships and exchange programs--remains on hold, limiting students' access to critical hands-on learning and professional development experiences. The NextGen PaCoN program is designed to make a lasting contribution to U.S. agriculture by preparing a skilled, future-ready workforce to support innovation, address emerging biosecurity threats, and serve farmers, stakeholders, and regulatory agencies. However, the ongoing funding uncertainty risks slowing this progress. Timely resolution and continued support are essential to ensuring the program can fulfill its mission and continue delivering high-impact, transformative experiences for students and the agricultural community. What opportunities for training and professional development has the project provided?At the graduate level: In Year 2, we recruited 10 graduate students (8 MS and 2 PhD). The NextGen PaCoN program provided these students with a variety of enriching experiences designed to strengthen their scientific, technical, and professional skills: Summer Exchange Program (SEP), July 2024 A three-week SEP was hosted at Oklahoma State University, engaging both graduate and undergraduate PaCoN students. The program included hands-on training in food safety, virology, molecular biology, sequencing, and bioinformatics. Students also explored Oklahoma's agricultural systems and connected with OSU faculty and peers. Graduate students from the University of Hawai'i participated, promoting cross-institutional learning and collaboration. The SEP boosted students' technical confidence, interdisciplinary awareness, and active lab engagement. Conference participation and presentation: Several graduate and undergraduate students presented research at national and regional conferences, including the APS Meeting in Memphis (August 2024) and the SRS-CTAHR Conference in Honolulu (April 2025). A few abstracts were submitted for upcoming ASM and APS conferences in Los Angeles and Honolulu (June-August 2025). Career Development Course: Led by Dr. Bingham, this required course helped students explore career options and develop job-readiness skills. It remains a key part of the PaCoN curriculum. Ghorban Miarkiani, a PaCoN PhD Student, participated in a travel fieldwork activity, operating as Pilot-in-Command while over on Big Island for half the trips duration. At the undergraduate level: Students from Leeward Community College, Oklahoma State University, and the University of Hawai'i at Manoa were actively engaged in food and agricultural biosecurity while also developing essential leadership and professional skills. Through the PaCoN program, students took initiative in planning and project activities, applying their creativity and deepening their understanding of biosecurity challenges. They gained experience in scientific communication by presenting their work to professional audiences, which helped them envision careers in science and public service. Students also expanded their networks by attending conferences and professional meetings, where they shared research findings and connected with experts--laying the foundation for future academic and career opportunities. Dr. James Stack visited Leeward Community College, delivered a seminar, and met with PaCoN undergraduate students to introduce them to career paths in agricultural biosecurity and their potential to support farmers, stakeholders, and the broader agricultural sector. At the high school level: Participation was focused through summer programs at Leeward Community College and Oklahoma State University, where high school students worked alongside undergraduates. They engaged in biosecurity-related activities, including lab experiments and field visits to farms, state agencies, and surveillance programs. These experiences offered hands-on learning and introduced students to real-world agriculture and biosecurity practices. Testimonials and feedback comments from the NextGen PaCoN students: "The PaCoN program transformed my academic and professional path. It funded my master's degree, accelerated graduation, and gave me hands-on experience in microbiology, genetics, and bioinformatics. Workshops and conferences boosted my skills, resulting in two manuscripts and national presentations. I now feel like a confident, competitive researcher". "Although I couldn't attend in-person events last year due to my pregnancy, being part of PaCoN still had a meaningful impact. Staying connected with the cohort and learning from their experiences kept me engaged and inspired. Now that I'm able to travel again, I'm excited to fully engage in upcoming opportunities and contribute more actively". "The NextGen PaCoN program has fundamentally transformed my PhD experience and career path in agricultural biosecurity by providing both financial stability and invaluable professional development opportunities. The program's financial support allowed me to focus on my research without seeking external employment. Through guest lectures, I broadened my understanding of global biosecurity challenges. The Plant Biosecurity in Theory & Practice workshop in Kansas proved particularly transformative, connecting me with biosecurity professionals from the Department of Homeland Security, USDA, and Australia, where I learned about fascinating career paths. These included DHS specialists tracking disease-carrying ungulates at borders and USDA data analysts monitoring trade activities. The opportunity to tour a working Biosafety Level 3 containment laboratory provided crucial hands-on experience that enhanced my technical understanding. Additionally, the NextGen conference in Washington D.C. significantly expanded my professional horizons. While there, I met professionals who helped me envision leadership possibilities within USDA and opened my eyes to career paths beyond traditional research. Overall, the NextGen PaCoN program has helped me understand how my research contributes to national and global agricultural security while providing a clear roadmap for my professional future." "As a two-year Master's student in the PaCoN, I have gained critical skills in protecting our nation's food supply and advancing agricultural independence. Through hands-on training in DNA sequencing, bioinformatics, and agrosecurity--along with internships and facility visits--I've learned to detect and mitigate threats to our food systems. This program gave me firsthand experience combating emerging plant diseases that could devastate American farms and food production. The technical expertise I've gained supports efforts to strengthen domestic biosecurity and reduce vulnerabilities in our agricultural supply chains. PaCoN has prepared me to help build a stronger, more self-sufficient agricultural future. Investing in programs like this empowers the next generation of farmers and leaders to safeguard America's food security, support rural economies, and restore key industries. My training showed how cutting-edge science can address real-world challenges--from improving crop resilience to preventing food safety threats. This isn't just education--it's a commitment to a resilient, independent America where our food supply stays secure, our farmers thrive, and our communities benefit from homegrown innovation and leadership". "PaCoN introduced me to food and biosecurity on a professional level. It connected my major, plant pathology, to real-world impact. Without the advertisement of this program, I would have been unsure of the path of how to apply myself to advance U.S. agriculture and my career. My education was increased multi-fold thanks to PaCoN. I learned lab protocols, procedures, and techniques, and now feel prepared for a meaningful career in U.S. agriculture. My education was increased multi-fold thanks to PaCoN". "Since pursuing my masters, I have learned much more of the fundamentals of biosecurity. It is not just the lectures that have provided me additional insight but the conferences, meeting likeminded individuals and the ability to network with others that has expanded my understanding and respect for efforts towards food security". Based on student testimonials and feedback, the NextGen PaCoN program has made a profound impact across academic levels--shaping career paths in agricultural biosecurity, food safety, and environmental sustainability. Through training, mentorship, fieldwork, and national exposure, students built practical skills and professional confidence. The program continues to develop a new generation of leaders dedicated to safeguarding U.S. agriculture and supporting local and national communities. How have the results been disseminated to communities of interest?The results were disseminated through peer-reviewed publications, presentations in scientific meetings, workshops and direct communications with farmers, stakeholders, extension agents and scientists. What do you plan to do during the next reporting period to accomplish the goals?Year 3 Plan: 1. Monthly Leadership Meetings: We will continue holding monthly leadership meetings to review project progress and address challenges. Meetings with the Steering Committee and Evaluation Committee are scheduled for Fall 2025. 2. Recruitments: We have 14 graduate students in the program. Our goal is to recruit 8-10 additional students by mid-2026; however, this is entirely dependent on confirmation of grant continuation. Undergraduate and high school recruitment at OSU for Summer 2025 has been canceled due to the grant freeze, but we plan to resume recruitment for Summer 2026. LCC will continue recruiting undergraduate and high school students for various project activities as long as funding remains active. 3. Graduation: Approximately 6-8 PaCoN graduate students are expected to graduate in Year 3 of the project. 4. Summer Exchange Program (SEP): This year's SEP, which was originally planned to be held at the University of Hawai'i, was canceled due to uncertainty surrounding grant continuation. We plan to organize the SEP at UH in Summer 2026, with participation from students at both UH and OSU. 5. National and International Internships: We plan to initiate arrangements for internships at both national and international levels in the coming semester, pending confirmation of grant continuation. At Oklahoma State University, Dr. Ma has already secured an international internship with New Zealand Food Safety, under the Ministry for Primary Industries, scheduled for Spring 2026 for three of her PaCoN students. Some of the internships originally planned for this year were cancelled or postponed due to the grant suspension. Other Co-PDs are also in contact with USDA/university laboratories for PaCoN students internships. 6. Biosecurity Workshop: The Summer 2025 Biosecurity Workshop was canceled. We plan to resume this activity in 2026, with all students participating in hands-on training in a containment facility at the Biosecurity Research Institute at Kansas State University. 7. Annual Meeting Presentations: Due to funding uncertainty, only a few PaCoN students will attend the APS meeting in Honolulu. We were also able to support one student to attend the ASM meeting in Los Angeles in Summer 2025. One PhD student will present at the 2025 Annual International Meeting of the American Society for Agricultural and Biological Engineers in Toronto, Canada. 8. USDA Opportunities: We will continue to explore and pursue USDA opportunities for PaCoN students, with plans to support their participation in upcoming USDA-organized events. 9. PaCoN Seminar Series: We plan to invite national and international speakers for the PaCoN Seminar Series in Fall 2025 and Spring 2026. 10. Publications: Several research manuscripts are currently in preparation and will be submitted for publication during Year 3 of the project.
Impacts
What was accomplished under these goals?
First PaCoN MS student graduated: December 2024: The first PaCoN student fully supported by the NextGen PaCoN program graduated with a 1-Year MS degree in Tropical Plant Pathology, specializing in bacterial genomics, diagnostics, and biosecurity. He gained valuable exposure through various program activities, completed all required coursework, and actively participated in multiple local and national events. Several additional MS students are expected to graduate in Year 3. Steering Committee Meetings with PDs and Co-PDs: In December 2024, Steering Committee members--Drs. James Stack, Jacqueline Fletcher, Doug Luster, and Darcy Oishi--held individual meetings with each PD and Co-PD to evaluate the project's Year One progress. These meetings were highly productive, with the committee expressing strong satisfaction with the progress made and offering valuable suggestions for further strengthening the project. This was a Zoom meeting. NextGen PaCoN All Members Meeting (November 4, 2024): In November 2024, we held a comprehensive PaCoN All Members Meeting, which brought together the PD, Co-PDs, Steering Committee, Evaluation Committee, and key project personnel. The purpose of this meeting was to provide a overview of project progress and establish a shared understanding among all members ahead of individual review meetings with each PD and Co-PD. During the session, members also discussed and tentatively scheduled the mid-project evaluation meeting. However, this mid-project meeting--originally planned for August 2025--was ultimately cancelled due to uncertainties surrounding the continuation of grant funding. Project Leadership Meeting (Monthly): Monthly project meetings, led by PD Dr. Arif, continued throughout Year Two with active participation from all Co-PDs and key personnel. These meetings served as a platform to review monthly progress, identify and address challenges, and plan upcoming activities. Regular discussions ensured alignment with project goals and timelines, promoted cross-team collaboration, and supported informed decision-making. Each meeting provided updates on individual contributions and facilitated strategic coordination to maintain project momentum and efficiency. Project Website and Social Media Accounts: All accounts were set up in year 1 to showcase project goals and activities, and to foster recruitment efforts. These platforms are being used and routinely updated to promote the project, share updates, and attract prospective students. Project Website: https://www.paconbiosecurity.net/ Project Website OSU: https://agresearch.okstate.edu/centers-institutes/bio-security/pecon/ Recruitment and Advertisement: Over the past year, several dynamic activities were undertaken to recruit students for the NextGen PaCoN project. Flyers were distributed to faculty for classroom promotion, the program was showcased at university-level events, and it was advertised at national conferences and workshops to attract students. Additionally, the project website was regularly updated to maintain visibility. A notable achievement was the student-led Earth Day event themed "Malama 'Aina and Food Biosecurity," aimed at educating the LCC campus community. The event promoted the program, supported recruitment efforts, and provided students with an opportunity to apply their leadership skills by planning and organizing the event entirely on their own. PaCoN Seminar Series: The PaCoN Seminar Series, held in Fall 2024, featured five invited speakers and eighteen student presentations. Integrated with the departmental seminar, it reached a broader audience and promoted the program to students from diverse backgrounds. The series enhanced academic and professional networking, provided access to expert knowledge, and helped prepare students for future careers. The Spring 2025 series was cancelled due to the grant suspension. Fall 2024, invited speakers included Dr. James Stack (Kansas State University) on emerging plant diseases, Dr. Astri Wayadande (OSU) on vector feeding, Dr. Mike Melzer and Dr. Daniel Jenkins (UHM) on vegetable propagation and surveillance technologies, Dr. Neha Potnis (Auburn University) on pathogen resilience, and Dr. Francisco Ochoa-Corona (OSU) on biosecurity as an integrative research field. Summary of Accomplishments: Although the grant suspension significantly impacted several planned activities, the project still made notable progress toward achieving its Year 2 milestones. Recruitment and student engagement efforts continued to advance across all partner institutions. At the University of Hawai'i at Manoa (UHM) and Oklahoma State University (OSU), NextGen PaCoN program fully supported 14 graduate students (11 MS and 3 PhD; equivalent to 7 FTEs at 0.5 FTE each) through graduate assistantships. At UHM, we also recruited and awarded scholarships to 8 undergraduate students participating in our two-year biosecurity exposure program. At Leeward Community College (LCC): The Summer ELP program enrolled 18 students (15 undergraduates and 3 high school students). The URE/Peer Mentor program during the Fall and Spring semesters enrolled 5 students. The Undergraduate Tuition Awards for Fall and Spring combined supported 46 seats (some LCC students participated in both semesters). At Oklahoma State University, they recruited and provided summer training to 18 students (10 undergraduates and 8 high school students). In total, we provided financial support and exposure to 87 students, surpassing our Year 2 target of 66 students. While we successfully recruited 14 (11 MS and 3 PhD) graduate students, short of our target of 18, this gap was primarily due to the grant suspension. We anticipate reaching the proposed number in the coming year. Although several activities were cancelled or postponed in Spring 2024, we plan to make up for them in Year 3 and continue building on the progress made thus far. Students demonstrated strong enthusiasm and engagement across a wide range of program activities. Their feedback reflects the meaningful impact these experiences had on their academic growth, career exploration, and professional development. Many students noted how the PaCoN experience expanded their understanding of biosecurity and its broader significance to agriculture and public health. As one student shared, "The PaCoN program has been a great opportunity. Beforehand, I only knew a little about biosecurity. This has been a great introduction to my understanding of the field." Another commented, "This program has been insightful and fun. Even though my major is in a different field, it made me aware of the similarities between plant and human health, and how interconnected the two really are." A particularly impactful reflection emphasized, "My highlight was learning how, like humans, plants also need people to study their pathogens and diseases to develop cures and resistances to protect our crops and food supply." Another wrote "This program for me was definitely a life changer". These reflections underscore the program's success not only in shaping student growth, but also in advancing the long-term goal of strengthening U.S. agriculture. By equipping future scientists, educators, and professionals with foundational knowledge and real-world experience in biosecurity, the PaCoN program is helping to build a resilient agricultural workforce capable of addressing emerging threats and safeguarding national food systems.
Publications
- Type:
Other Journal Articles
Status:
Accepted
Year Published:
2025
Citation:
Xing Wei, Tomie Vowell, Scott Nikaido, Jordie Ho*, Alexandra Kong, Keith Weiser, Daniel Jenkins, and Michael Melzer (2025X) Evaluating the persistence of pyrethrin and pyrethroid insecticides in coconut palm (Cocos nucifera) canopies for coconut rhinoceros beetle (Oryctes rhinoceros) management. Proceedings of the Hawaiian Entomological Society, 57:7-16 (in press).
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2025
Citation:
Arif M, Zeng Q, Dobhal S (2025). Editorial: Research in the identification and control methods of rot diseases in plants. Frontiers in Microbiology, doi: 10.3389/fmicb.2025.1601422
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2025
Citation:
Lutgen, H., Vowell, T., Marquez, J., Ho, J.*, Matsunaga, J., and Melzer, M. 2024. First report of Xylella fastidiosa subsp. sandyi infecting oleander (Nerium oleander) in Hawaii, USA. New Disease Reports 2024;50e12308 DOI:10.1002/ndr2.12308
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
*Krakowiak S, Dobhal S, Arif M (2024). Genomic Insights into Xanthomonas axonopodis pv. vasculorum: causative agent of sugarcane gumming disease. APS Plant Health 2024, July 27-30, Memphis, Tennessee.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Arif M, Dobhal S, Ma LM, Stack JP (2024). Emerging strains and their multi-trophic interactions threatening food safety and biosecurity. XX International Plant Protection Congress, July 1-5, Athens, Greece.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
*Tamayo EP, *Abes Z, Neupane K, Kae H. (2024). Isolation and characterization of a Pectobacterium isolate from a radish (Raphanus sativus) Root in Hawaii. APS Plant Health 2024, July 27-30, Memphis, Tennessee.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
*Abes Z, Neupane K, Kae H. (2024). Exploring the nature of the symbiotic relationship between methylotrophic bacteria on native hawaiian plants. APS Plant Health 2024, July 27-30, Memphis, Tennessee.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
*Tamayo EP, Neupane K, Kae H. (2024) Isolation & Characterization of a Pectobacterium Isolate from a Daikon Radish (Raphanus sativus) Root in Hawaii. Presented at the NDI-STEM Conference, Oct 30-Nov2, 2024, Phoenix, Arizona.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
*OLeary E, Macapagal C, Kae H, Neupane K. (2024). An Evaluation of Genetic Diversity in pure Rhizobia cultures isolated from cultivated and uncultivated legumes from Oahu. Presented at the NDI-STEM Conference, Oct 30-Nov 2, 2024, Phoenix, Arizona.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
*Abes Z, Neupane K, Kae H. (2024). Exploring the Nature of the Symbiotic Relationship between Methylotrophic bacteria on native Hawaiian plants. Presented at the NDI-STEM Conference, Oct 30-Nov2, 2024, Phoenix, Arizona.
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2025
Citation:
Paryavi, M., Weiser, K., Melzer, M., Crook, D., Ramadugu, C., and Jenkins, D.M. 2025. Programmable LED array for evaluation artificial light sources to improve pest trapping. Insects 16(2) 170 https://doi.org/10.3390/insects16020170
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2025
Citation:
Paryavi, M., Weiser, K., Melzer, M., Ghorbani, R., and Jenkins, D. 2025. Autonomous cellular-networked surveillance system for coconut rhinoceros beetle. Computers and Electronics in Agriculture 235:110310 https://doi.org/10.1016/j.compag.2025.110310
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2025
Citation:
*Ku C, Camann I, Chang JYX, *Stewart V, *Yamamoto, *Syhlman D., Olarti J, Chung WH, *Krakowiak S, Arizala D, Dobhal S, Jenkins D, and Arif M. Genome informed LAMP assay for rapid and specific detection of Xanthomonas albilineans. University of Hawaii CTAHR Student Research Symposium, April 4, 2025, Honolulu, HI., USA.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2025
Citation:
*Syhlman D, Arizala D, Dobhal S, Alvarez AM, Arif M (2024). Pan-genome insights into the evolutionary significance of the megaplasmid in Ralstonia pseudosolanacearum. SRS-CTAHR Symposium 2025, April 10-12, Honolulu. HI.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2025
Citation:
*Stewart V, Arizala D, Dobhal S, Arif M (2025). Phenotypic and genomic diversity of Pectobacterium brasiliense strains isolated from various hosts in Hawaii. SRS-CTAHR Symposium 2025, April 10-12, Honolulu. HI.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Arif M, Stack JP, Dobhal S, Bingham J-P, Fletcher J (2024). Fostering research in graduate teaching: the excitement of transforming theoretical foundations into peer-reviewed publications. APS Plant Health 2024, July 27-30, Memphis, Tennessee.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
*Syhlman D, Arizala D, Dobhal S, Alvarez AM, Arif M (2024). Pan-genome analysis reveals a high diversity in the chromosome and megaplasmid of Ralstonia pseudosolanacearum. APS Plant Health 2024, July 27-30, Memphis, Tennessee.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
*OLeary E, Macagapal C, Kae H, Neupane K. (2025). Comparison of the genetic diversity in Rhizobia isolated from cultivated and uncultivated legumes grown on Oahu. Presented at the INBRE Biomedical Symposium, April 11, 2025, Honolulu, HI.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
*Simzer C, Li H, Kae H, Neupane K, Berestecky J, Haun B, Ross M. (2025). Use of Molecular Techniques to Investigate Hybridization Between Two Atriplex Species in Hawaii. Presented at the INBRE Biomedical Symposium, April 11, 2025, Honolulu, HI.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2025
Citation:
Paryavi, M., *Miarkiani G, and Jenkins DM. Automated delineation of coconut rhinoceros beetle with a distributed surveillance system and machine vision tools. (Abstract accepted for presentation at Annual International Meeting of the American Society for Biological and Agricultural Engineers, July 14, 2025, Toronto, Ontario, Canada).
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2025
Citation:
*Stewart V, Arizala D, Dobhal S, Arif M (2025). Comparative genomics and phenotypic analysis of Pectobacterium brasiliense strains isolated from various hosts in Hawaii. (Abstract accepted for presentation at APS Plant Health 2025, August 1-5, Honolulu. HI.)
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2025
Citation:
*Ku C, *Uzzamman MA, Dobhal S, Arif M, Jenkins D (2025). Electroflotation cartridge for rapid microbial enrichment to improve molecular detection limits for Dickeya dianthicola and Escherichia coli. (Abstract accepted for presentation at APS Plant Health 2025, August 1-5, Honolulu. HI.)
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2025
Citation:
*Stewart V, Arizala D, Dobhal S, Arif M (2025). Phenotypic and genomic diversity of Pectobacterium brasiliense strains isolated from various hosts in Hawaii. (Abstract accepted for presentation at ASM Microbe 2025, June 19-23, Los Angeles, CA).
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2025
Citation:
*Miarkiani G, Paryavi M, and Jenkins DM. Distributed system for detection of coqui frogs in Hawaii. (Abstract accepted for presentation at Annual International Meeting of the American Society for Biological and Agricultural Engineers, July 14, 2025, Toronto, Ontario, Canada).
|
Progress 06/01/23 to 05/31/24
Outputs
Target Audience:The target audience for this reporting period includes graduate, undergraduate, and high school students; community college students in the UH System; Native Hawaiian and Pacific Islanders (NHPI); Tribal Native Americans (TNA); federal and state agencies; USDA APHIS; Hawaii Department of Agriculture; Hawaii Department of Land and Natural Resources; Hawaii Invasive Species Council; Coordinating Group on Alien Pest Species; University of Guam and other universities on the mainland; Northern Marianas College; participants of the American Phytopathological Society's Annual Meeting 2023; participants of Biosecurity workshop at Kansas State University, and NextGen PaCoN seminar participants from various national and international universities and institutes. Changes/Problems:Recruitment Challenges and Plans: We faced recruitment challenges at all three institutions due to delayed funds and the lengthy process of finalizing the subaward to Oklahoma State University (OSU). UHM: Recruitment was limited (only 3 were recruited) due to the short time available for advertising. However, UHM is set to achieve its proposed numbers in Year 2. OSU: Received the subaward in mid-December 2023, preventing student recruitment for Year 1 but plans to recruit in Year 2. LCC: Dr. Neupane and his team filled 11 out of 16 seats for the Summer ELP 2024. So far, they have successfully recruited 39 out of 40 candidates and are on track to meet their Year 2 goals. In conclusion, we anticipate no further issues and expect to make the proposed progress in the coming years. What opportunities for training and professional development has the project provided?At the graduate level: In Year 1, we recruited only three students (3 MS and 1 PhD) due to a delay in funding. However, all three students were exposed to various career development opportunities. Three students participated in a week-long Biosecurity Workshop at KSU, where they experienced experiential learning. They were exposed to different techniques used in biosecurity and gained experience working in a containment facility. They met with leading experts in biosecurity and developed a network with other participants from across the globe. All three students also participated in the NextGen USDA Agriculture Outlook Forum, where they learned about different career opportunities at USDA. Two students participated in a four-day Bioinformatics Data Science Workshop at the University of Hawaii Manoa, where they were introduced to bioinformatics and R modules. PaCoN students also took a career development course under Dr. Bingham to learn about job opportunities and how to prepare for the job market. Dr. Bingham's course is part of the PaCoN curricula. At the undergraduate level: Students exposed to the field of food biosecurity while also focusing on their personal growth and development, preparing to become future leaders in their field. They took leadership in developing event activities, showcasing their skills while learning deeply about food biosecurity. One student applied classroom knowledge to real-world issues, enhancing professional writing and mentoring others, amplifying the impact. Students also learned to communicate their work to professional scientists, envisioning a future science career and gaining valuable networking opportunities. They participated in conferences and meetings and submitted their research outcomes to the scientific meetings. At the high school level: Participation was limited to the Summer ELP at Leeward Community College, where high school students worked alongside undergraduates. They engaged in biosecurity-related activities through lab experiments and field visits to agricultural farms, state agencies, and surveillance programs. This exposure provided them with hands-on experience and insights into biosecurity practices. A Testimonial from a NextGen PaCoN Student: "My time beginning the NextGen PaCoN program, my world and the life I've been living has changed rapidly, and in my opinion for the better. The NextGen PaCaN has provided me with opportunities I could've only dreamed of just a few months ago. In just a few short months, I've not only gained invaluable knowledge about agricultural biosecurity, but I've also had the chance to connect with USDA officials and peers in our field. This has allowed me to see the broader impact of our work on both a national and global scale. One highlight was the week-long workshop at the Biosecurity Research Institute at Kansas State University, where I received hands-on training in a containment facility and had the unique opportunity to meet world leaders in biosecurity--an experience that was truly one-of-a-kind. Additionally, the three-week Summer Exchange Program at the Institute of Biosecurity and Microbial Forensics at Oklahoma State University provided me with cutting-edge training and skill development, preparing me to contribute meaningfully to the field. The NextGen PaCoN program has not only broadened my horizons but has also empowered me to pursue my passion for agriculture". How have the results been disseminated to communities of interest?The results were disseminated through peer-reviewed publications and presentations in scientific meetings and workshops. What do you plan to do during the next reporting period to accomplish the goals?Year 2 Plan: Monthly Leadership Meetings: Continue monthly leadership meetings to review project progress/challenges and hold meetings with the Steering Committee and Evaluation Committee in Fall 2024. Recruitments: We have finalized 10 graduate students who will soon join the program. At LCC and OSU, we have a strong pool of undergraduate applicants, and high school applications for the Summer ELP program at LCC are high. OSU is still receiving applications, and we are confident in achieving our recruitment goals for Year 2. We will continue our recruitment efforts for Year 3. Summer Exchange Program at OSU: A three-week summer exchange program at OSU will begin on July 8, 2024, with all graduate students from UHM participating. National Internships: We are coordinating with collaborators for six-week national internships for our two-year MS students. International internships for PhD students will take place in 2026. Biosecurity Workshop: In summer 2025, all PG students (about 10) will participate in biosecurity workshop at KSU. Annual Meeting Presentations: All postgraduate students and two undergraduate students will present at the APS Meeting 2024 in Memphis, Tennessee. USDA Opportunities: We will continue to explore USDA opportunities for NextGen students, planning to send students to participate in upcoming USDA-organized events. Seminar Series: We will invite eight national and international speakers for the Fall 2024 seminar series and another eight for Spring 2025. Publications: Multiple research articles are in preparation and will be communicated in Year 2.
Impacts
What was accomplished under these goals?
The NextGen PaCoN Kickoff Meeting: The kickoff meeting was organized at the 2023 APS Meeting in Denver, CO. The PD, Co-PDs, members of the Steering and Evaluation Committees, and other key personnel of the project attended. The meeting was a great success, during which the framework and execution plan of the project were discussed in detail. Attendees engaged in productive discussions, outlined specific goals and milestones, and established clear roles and responsibilities. The collaborative atmosphere fostered a shared vision and commitment to the project's objectives, setting a strong foundation for future progress. Project Leadership Meeting (Monthly): The project's monthly meetings are led by PD Dr. Arif. All Co-PDs and key personnel participate to discuss progress made over the past month, address any challenges faced, and plan for the upcoming month. These discussions ensure alignment with the project's overall goals and milestones. The meetings foster collaboration, provide updates on each member's contributions, and facilitate strategic planning to keep the project on track and moving forward efficiently. Project Website, Logo, and Social Media Accounts: The NextGen PaCoN project website was designed and launched to showcase project goals and activities, and to foster recruitment efforts. A logo design competition resulted in over 100 submissions, from which the final logo was selected. Social media accounts on X, Facebook, Instagram, and LinkedIn have been established to disseminate information and engage a broader audience. These platforms are actively used to promote the project, share updates, and attract prospective students, significantly enhancing the project's visibility and impact. Project Website: https://www.paconbiosecurity.net/ Project Website OSU: https://agresearch.okstate.edu/centers-institutes/bio-security/pecon/ Recruitment and Advertisement: Over the past year, several dynamic activities were conducted to recruit students for the NextGen PaCoN project. A central project flyer, alongside those from Leeward Community College and Oklahoma State University, were distributed via social media, emails, university professors, collaborators, state agencies, APS meeting job boards, and various workshops and meetings. Additionally, PaCoN recruitment surveys were developed for UG/PG students, and active engagements with students at colleges and universities were pursued. Participation in conferences and workshops, further enhanced visibility. Strategic trips to Guam and Northern Marianas College resulted in two new student recruits. Networking with other project leaders from NIH- and NSF-funded initiatives expanded our outreach. One notable achievement includes a student-supported initiative at Leeward Community College, where a Registered Independent Student Organization (RISO) called the Food Biosecurity Club was established. This club provides field-related exposure through networking, field activities, and volunteering, effectively educating and inspiring prospective students to join PaCoN. The LCC Office of Planning, Policy, and Assessment (OPPA) also highlighted PaCoN grant activities in their newsletter, boosting the program's profile. Curricula Development: The development of specialized curricula for the 1-Year MS, 2-Year MS, and PhD programs as part of the NextGen PaCoN program at the University of Hawaii at Manoa and Oklahoma State University has significantly enhanced the academic framework for students pursuing careers in agriculture and biosecurity. These curricula combine rigorous theoretical knowledge with practical applications, equipping students with the skills needed to address real-world challenges. The inclusion of undergraduate courses, such as "Agrosecurity and Food Safety," and graduate courses like "Molecular Diagnostics: Principles and Practices," fosters student engagement and cultivates critical thinking. Additionally, hands-on experiences in summer programs further bridge the gap between academic learning and industry demands. The curricula are designed to inspire students and prepare them for meaningful contributions in biosecurity, increasing their job opportunities both in the U.S. and internationally. PaCon Seminar Series: Launched in Fall 2023, the PaCoN Seminar Series features renowned national and international scientists who share their expertise and engage directly with students, fostering an interactive learning environment. This initiative has significantly enhanced the program's visibility among leading researchers and institutions, creating a robust professional network. It effectively promotes the program to a diverse student audience, attracting interest and participation from various educational institutions. By facilitating interactions between students and experts, the seminars enrich the academic and professional networks of participants, further elevating the program's impact. In conclusion, the PaCoN Seminar Series is an important element of the educational experience, especially for graduate students. It provides them with access to expertise, fosters critical professional networks, and prepares them for future career success. This initiative not only enhances the academic quality of the program but also positions students to excel in their respective fields. According to students, the seminars are helping them broaden their understanding, better design their research objectives, and expand their professional networks, which could assist them in securing jobs in agriculture. In Fall 2023, four speakers presented seminars via Zoom, including Dr. Tiffany Lowe-Power (UC Davis) on Ralstonia wilt pathogens, Jonathan M. Jacobs (Ohio State University) on Xanthomonas surveillance, Dr. Christian D. Cruz (Purdue University) on sensor and modeling, and Dr. Andres Espindola (Oklahoma State University) on bioinformatics tools for detection. In Spring 2024, eight speakers participated, including Dr. Yasmin Rivera (USDA) on diagnostics, Dr. Grant Smith (New Zealand) on myrtle rust's threat to biodiversity, Dr. Erica Goss (University of Florida) on pathogen emergence, Dr. Edel Perez-Lopez, (Canada) on food security, Dr. Guillaume Bilodeau (Canada) on surveillance, Dr. Sanjay Antony Babu (Texas A&M University) on wilt microbiomes, Dr. Francisco J. Flores (Ecuador) on banana wilt and Dr. Jeff Chang (Oregon State University) on evolution. Summary of Accomplishments: All the proposed project activities are on track. Our recruitment efforts are progressing well. Although MS/PhD recruitment hasn't fully met our targets in Year 1, undergraduate and high school student recruitment has been satisfactory. At Leeward Community College, the Summer ELP filled 11 out of 16 seats; LCC URE/Peer Mentors combined for the Fall and Spring semesters filled 4 out of 4 seats; and LCC Undergraduate Tuition Awards combined for the Fall and Spring semesters filled 24 out of 20 seats. Therefore, 39 out of 40 awards were allocated during this Year 1. Students have shown great enthusiasm for this program and provided excellent feedback. Some of their feedback states "The NextGen PaCoN program has not only broadened my horizons but has also empowered me to pursue my passion for agriculture", "PaCoN truly opened doors for me in and out of the classroom", "I learned that Food Biosecurity is very important to our life and did not realize different areas of careers and everyday life could link back to Food Biosecurity" etc. Our seminars attract a good number of attendees, leading to productive discussions with each speaker. Our team is committed to fostering a supportive and dynamic learning environment, ensuring that students gain valuable exposure and practical experience in biosecurity. As we continue to build on our successes, we remain focused on enhancing our recruitment efforts, expanding our reach, and strengthening the impact of the NextGen PaCoN program.
Publications
- Type:
Journal Articles
Status:
Submitted
Year Published:
2024
Citation:
Marabella M, Howard J, Bhandari S, Do S, Montoya-Pimolwatana M, Dou Y, Dobhal S, Arizala D, Montesinos S, Andreason SA, Ochoa-Corona F, Bingham JP, Odani J, Jenkins D, Ma LM, Fletcher J, Stack JP, Arif M (2024). Loop-mediated Isothermal Amplification (LAMP) assay for reliable detection of Xanthomonas axonopodis pv. vasculorum. BioRxiv. doi: https://doi.org/10.1101/2024.02.07.579270
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Arif M (2024) Emerging Strains and Multi-Trophic Interactions: A Serious Threat to Food Safety and Biosecurity? at Plant Biosecurity in Theory and Practice workshop, Biosecurity Research Institute, Kansas State University, on May 24, Manhattan, KS
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Tamayo EP, Abes Z, Neupane K, Kae H. (2024). Isolation and characterization of a Pectobacterium isolate from a radish (Raphanus sativus) root in Hawaii. Presented at the 2024 ASM-HI at John A. Burns School of Medicine on April 27, 2024, Honolulu, HI.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Tamayo EP, Abes Z, Neupane K, Kae H. (2024). Isolation and characterization of a Pectobacterium isolate from a radish (Raphanus sativus) root in Hawaii. Presented at the 2024 INBRE Biomed Research Symposium at John A. Burns School of Medicine on April 19, 2024, Honolulu, HI.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Arif M, Stack JP (2023). Advancement in plant pathogen diagnostics in high-throughput sequencing era. Presented at ICPP 2023, Lyon, France.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
Syhlman D, Arizala D, Dobhal S, Alvarez AM, Arif M (2024). Pan-genome analysis reveals a high diversity in the chromosome and megaplasmid of Ralstonia pseudosolanacearum. (accepted for presentation at the APS Plant Health 2024, July 27-30, Memphis, Tennessee).
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
Krakowiak S, Dobhal S, Arif M (2024). Genomic Insights into Xanthomonas axonopodis pv. vasculorum: causative agent of sugarcane gumming disease. (accepted for presentation at the APS Plant Health 2024, July 27-30, Memphis, Tennessee).
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
Arif M, Dobhal S, Ma LM, Stack JP (2024). Emerging strains and their multi-trophic interactions threatening food safety and biosecurity (presenting at the XX International Plant Protection Congress, July 1-5, Athens, Greece).
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
*Tamayo EP, *Abes Z, Neupane K, Kae H. (2024). Isolation and characterization of a Pectobacterium isolate from a radish (Raphanus sativus) Root in Hawaii. Accepted for Presention at the APS Plant Health 2024, July 27-30, Memphis, Tennessee.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
Abes Z, Neupane K, Kae H. (2024). Exploring the nature of the symbiotic relationship between methylotrophic bacteria on native hawaiian plants. Accepted for presentation at the APS Plant Health 2024, July 27-30, Memphis, Tennessee.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Opportunities in Biosecurity, at Plant Biosecurity in Theory and Practice workshop, Biosecurity Research Institute, Kansas State University, Manhattan, KS, USA
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
Arif M, Stack JP, Dobhal S, Bingham J-P, Fletcher J (2024). Fostering research in graduate teaching: the excitement of transforming theoretical foundations into peer-reviewed publications. (presenting at the APS Plant Health 2024, July 27-30, Memphis, Tennessee).
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