Performing Department
(N/A)
Non Technical Summary
Whey waste is a major challenge for waste treatment due to its large production globally. Whey contains key nutrient proteins which can be recovered and valorized as food products. However, recovering high-purity food-grade protein from whey can be very challenging, due to the complexity of the whey composition. Current protein purification technologies are highly costly step for whey valorization, due to the high consumption of chemicals, water, and the large number of separation processes required. The development of new purification technologies tailored at whey protein recovery can greatly benefit the environment, agriculture, and society by providing a sustainable source of food products, while at the same time treating a major waste ource. Electrochemically-mediated technologies have the potential to greatly improve sustainability of protein separations by removing the need for regeneration chemicals, extensive elution, or thermal input.Redox-electrodialysis can provide a modular platform for whey protein valorization, due to the use of low voltage - allowing for control of pH, low energy-consumption, and selective recovery. Here, we seek to develop a modular, molecularly-selective system for the recovery of whey proteins from acid, sweet, and salty whey, through desalination of the inorganic salts and the purification of valuable nutrient proteins. We design a new modular membrane-electrode arrangement for multicomponent recovery, and study the structure-function relationship of size and charge of proteins with selectivity. Finally, we seek to improve the robustness of the redox-flow moiety to eliminate crossover and enable long-term operation. The combination of these tasks will lead to a modular, energy and resource efficient method for producing valuable food products from whey streams. Our project involves a close partnership with food manufacturing end-users, including dairy manufacturers and food waste.
Animal Health Component
0%
Research Effort Categories
Basic
50%
Applied
30%
Developmental
20%
Goals / Objectives
The overarching goal of the project is to innovate agricultural food manufacturing in the U.S. by developing more efficient technologies for dairy manufacturing, through the recovery and valorization of proteins from whey waste. We propose to develop redox-polymer driven electrodialysis flow cellsto enable multi-component separation of whey proteins and salts for food manufacturing through the rational arrangement of selective membranes and redox-channels. The project pursues an integrated approach between process engineering, systems design, and redox materials synthesis, for the implementation of a novel separation technology in food manufacturing through whey protein recovery and purification. The following objectives will be followed in the current proposal:Objective 1.Investigate various whey waste compositions (e.g., acid, sweet, salty whey) to screen and source whey proteins that are selectively separable for valorization.Objective 2.Develop electrochemically-mediated flow system based on redox electrolyte for continuous and selective salt and protein separation of whey waste stream.Objective 3.Synthesize and engineer green redox polymers to facilitate usage of nanofiltration membranes and minimize crossover and interactions with proteins.Objective 4.Technoeconomic analysis of the redox-ED system and evaluation of the multi-channel system for relevant food manufacturing samples from the dairy industry.
Project Methods
Methodology for Objective 1: First, we seek to select, characterize, and create a library of whey waste compositions to benchmark both industrial whey samples from collaborators, and formulate a model to represent various types of acid, sweet, and salty whey wastes. We then aim to establish characterization methods to understand the composition and separation parameters of whey waste based on the charge, mass, and polarity of complex molecules present in whey. Next, we seek to: (i) Benchmark compositions from industrial whey samples from dairy manufacturers, and (ii) Gather data to formulate synthetic whey waste of target components. Accurate quantitation of the composition of whey before and after treatment will be key for the remainder of the project. Analytical accuracy >95% for both salt and protein concentration measurements are envisioned using chromatography and ion-conductivity measurements.Methodology Objective 2: We aim to fabricate, design and develop continuous and scalable electrochemically-driven redox flow system to separate salt and protein by providing multiple flow channels that are distinguished by membrane separators. We seek to draw structure-function correlations between operating conditions of flow, membrane properties, and protein parameters (isoelectric point, size, charge, and hydrophobicity), as well as identify potential ranges for protein stability. In addition, we will achieve fractionation of proteins using membranes with a wide range of molecular weight cut off and functionality. Target protein recovery from the total whey mixture will be aimed at 80%, with individual component protein purity >95% by the end of the project.Methods/Efforts from Objective 3:Research Task 3a: Design and synthesize water soluble redox-active polymers with tunable size, which can be effectively retained by low-cost nanofiltration membranes. Research Task 3b: Tuning of charge and electrochemical properties of redox polymers to minimize denaturation of valuable proteins and maximize long term operational stability. Expected Outcomes of Objective 3: Successful completion of Objective 3 will result in a versatile platform of watersoluble redox copolymers for usage in nanofiltration membrane based electrochemically-mediated flow systems, with a structure optimized for high separation performance, low membrane crossover and improved compatibility with a wide range of proteins. We will aim at non-detectable cross-over (100% retention) of the redox-species and >95% retention of charge for >10,000 hours of operational stability in redox-cycling.Methods/Efforts from Objective 4: Research Task 4a. Operate developed flow system using conditions suitable for industry, including real world whey protein waste sourced from industrial collaborators. Research Task 4b. Develop a process model for scaleup, and conduct preliminary technoeconomic analysis (TEA) for estimation of cost, recovery yields and operating costs for whey protein separation for end-user food manufacturing. Expected Outcomes of Objective 4: Successful completion of research Tasks 4a and 4b will provide economic feasibility and inform optimal electrochemical system arrangements of the system based on the whey waste sources. The combination of real whey waste valorization in Task 4a with techno-economic analysis in Task 4b will provide a comparative landscape to evaluate the industrial applicability of the electrochemically-mediated ED system.