Source: TEXAS TECH UNIVERSITY submitted to NRP
CHARACTERIZATION OF 3D PRINTED PROTEIN INKS WITH CUSTOMIZATION USING MACHINE LEARNING
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1030247
Grant No.
2023-67017-40745
Cumulative Award Amt.
$299,620.00
Proposal No.
2022-09135
Multistate No.
(N/A)
Project Start Date
Sep 1, 2023
Project End Date
Aug 31, 2026
Grant Year
2023
Program Code
[A1364]- Novel Foods and Innovative Manufacturing Technologies
Recipient Organization
TEXAS TECH UNIVERSITY
(N/A)
LUBBOCK,TX 79409
Performing Department
(N/A)
Non Technical Summary
3D food printing is a new technology that allows for the automatic creation of foods with appealing shapes. Food printing can benefit athletes by creating foods with optimized nutrition, benefit children for printing healthy foods in fun shapes, and benefit those with chewing/swallowing difficulties by producing more appealing soft foods. Additionally, the technology can be a convenient way for serving dinner through automated food production at home. Unfortunately, many printed foods must have additives included with poor taste and nutrition. Currently, there are no established procedures for creating healthier foods due to difficulties in understanding how different food ingredients relate to print quality. We are proposing the creation of a machine learning model that can help predict how well foods will print when new ingredients are combined. The machine learning model will be trained using a series of physical food experiments and food taste testing studies. Our research aims to create healthy protein inks, with a focus on novel oleocolloid/hydro-olleocoloids (OC/HOCs) that will enable delivery of highly nutritious foods in appealing shapes with a consistency similar to butter/peanut butter. Overall, this approach could stimulate economic activity for businesses focused on automated food production while also resulting in nutritious recipes to poromote healthier eating.Our approach for carrying out the research consists of two objectives. The first objective is to determine how to print healthier protein inks. Research methods will consist of altering ingredients of protein inks by changing the amounts of each ingredient such as whey/soy proteins, canola/soy oils, rice bran wax, and water. These foods will be measured for their physical properties, for example their firmness or thermal properties, that informs how well they may print. Foods will be printed with the accuracy of shapes measured using a newly developed computational approach. The results of the objective will provide an understanding of how different combinations of healthy ingredients affect food printing accuracy. A second objective will be carried out to measure how well consumers enjoy printed foods and if these relationships can be predicted using machine learning. Once a machine learning algorithm is developed, foods will be printed on a customized basis to demonstrate how the technology could be used for creating optimized foods for individual consumers. The research is anticipated to provide societal benefits for delivering healthier, more appealing foods that are automatically personalized for the needs of consumers.
Animal Health Component
30%
Research Effort Categories
Basic
70%
Applied
30%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
50150102020100%
Knowledge Area
501 - New and Improved Food Processing Technologies;

Subject Of Investigation
5010 - Food;

Field Of Science
2020 - Engineering;
Goals / Objectives
Our long-term goal is to deliver highly nutritious sustainable foods on demand through 3D printing that are optimized for manufacturability, sensory appeal, and nutrition. Our goal for this proposal is to characterize protein inks including OC/HOCs for their rheological and sensory properties, and demonstrate the feasibility for machine learning to deliver personalized printed products based on characterized relationships.We will pursue the proposal goal with the following specific objectives:Specific Objective 1: Printability characterization for protein inks. Our working hypothesis is the printability and texture of protein inks is controllable by altering ink composition. The hypothesis will be tested by creation of diverse protein inks with rheological property characterization and computer assisted assessment of printed shape fidelity.Specific Objective 2: Machine learning for personalized manufacturing. Our working hypothesis is machine learning can model complex relations among food ingredients, printability, and sensory properties in a predictable manner for customization. The hypothesis will be tested by measuring relevant properties and validating how ML enables 3DFP personalization.
Project Methods
The project will be conducted by carrying out a series of experiments for two objectives as follows:Broadly for Objective 1: Printability characterization for protein food inks, the following three sets of experiments will be carried out: Experiment 1.1 OC/HOC (high-protein oleocolloids/hydro-oleocolloids) matrix formation, Experiment 1.2 Printability assessment, and Experiment 1.3 Rheological/texture properties. Experiment 1.1 efforts will focus on the creation of novel food mixtures that are assessed using established methods to analyze compositional elements, oil binding capacity, thermal properties, and texture analysis. Experiment 1.2 efforts will create a design mapping of fidelity for printed foods using a newly created method for computationally assessing the difference between 3D scans of printed foods and their intended digital design. Experiment 1.3 efforts will characterize newly developed protein inks using rheological and texture analysis for food mixtures prior to 3D printing. Project evaluation will have milestones set for completion of each experiment once data is collected as a measurement of success. For Experiment 1.1 data will consist of ingredient compositions for food and their measured properties, for Experiment 1.2 data will consist of a mapping of ingredient compositions to achieved print fidelity, and for Experiment 1.3 data will consist of measured rheological and textural properties for each considered food mixture.Broadly for Objective 2: Machine learning for personalized manufacturing, the following three sets of experiments will be carried out: Experiment 2.1 Consumer ratings for protein inks, Experiment 2.2 Machine learning model for printability and likeability, and Experiment 2.3 Personalized printing with validation. Experiment 2.1 efforts will focus on collecting the sensory ratings of panelists using established methods for novel 3D printable foods. Experiment 2.2 will focus on the creation and characterization of a novel machine learning algorithm that takes food ingredients as inputs, assesses their rheological and sensory properties, and then predicts printability and likeability. Experiment 2.3 will use the newly developed machine learning model to create pareto fronts through multi-objective optimization to demonstrate a proof-of-concept personalization for consumers through customizable 3D printed foods. Evaluation of the project will have milestones for completion of each experiment. Data for Experiment 2.1 sensory studies will be collected according to procedures approved by the Institutional Review Board, Experiment 2.2. machine learning will generate digital data and models of relationships between food printing inputs and outputs, and Experiment 2.3 personalized printing will result in digital optimization data describing a multi-objective design space and images of representative foods as a proof-of-concept for customization.Educational efforts throughout the project will be made to integrate science-based knowledge from the proposed work in courses and outreach programs at Texas Tech University (TTU) currently carried out by the PI. In courses lectures will provide an overview of 3D printed foods and their relevance to health applications which the PI teaches in undergraduate and graduate engineering design courses, with new modules for lectures incorporated by this research. In outreach efforts the PI will include slides demonstrating 3D food prints in lectures educating K-12 STEM students and the public about the benefits of nutritious foods and possibility of nutritious food delivery through 3D printing. Evaluation will be conducted through TTU supported mechanisms for assessing learnings in the classroom and outreach, such as surveys and formative/summative evaluations of learnings.

Progress 09/01/23 to 08/31/24

Outputs
Target Audience:The research matters on a social level for persons in need of personalized nutrition and customized food delivery, the reason why it is helpful is because the soft delivery of nutrient dense foods on a personalized basis can aid those in health application such as treating dysphagia or eating disorders. Personalized nutrition can aid athletes in personalized nutrition. The delivery method is also applicable for military applications where deployed persons may need efficient and personalized foods or in space exploration missions.Additionally, 3D food printing is an efficient means to deliver foods to address allergy concerns and help nutritious foods reach underserved ares. Work has been conducted with a target audience of student and lab researcher instruction to teach food engineering and design principles for addressing these health issues. Classroom instruction for 3D food printing design principles for 3D printing with a highlight of food applications were provided in undergraduate course for Medical Design and Entrepreneurship for Mechanical Engineers and graduate level course Advanced Engineering Desing for Mechanical Engineers. One undergraduate and two Master's students have conducted research studies in the PD's laboratory for food printing that has included formal instruction for food printing, texture analysis, food ink characterization, and dimensional analysis for printed foods. internships and MS internships in the lab. has been covered in University courses. Food engineering, engineering design, industry contacts. Contact and networking were conducted with two 3D food printing companies for informal education and learning regarding translational research from food printing research to practice. Outreach was conducted through the Research Made Accessible program for informal education of one high school student regarding research in food engineering and printing. Changes/Problems:A key goal in this Seed Grant project is establishing a collaboration with comparable experimental setups between laboratories at TTU and OSU which has required a setup period in establishing regular communication among researchers at each institution and shared protocols. We have successfully established a productive collaborative environment with some aspects of setups and logistics in this new collaboration requiring further time for training and setup that have created a short delay on expenditures at OSU while Dr. Egan and Dr. Maleky have focused on establishing the setup and protocols at TTU. We plan to request a no-cost extension to effectively use the remaining funding for carrying out the project for OSU goals that build from TTU work in initial food ink characterization. Our work to-date has been carried out according to the planned grant work as described in the accomplishments section and we anticipate the continued work in the next reporting period will continue building in the direction of the original proposal. Assessment of printability was planned with 3D scanning software, however, the data with 3D scanning was not consistent and difficult to analyze in comparison to 2D imaging that has greater reliability and requires less computational resources. We plan to conduct automated printability assessment with 2D imaging approach to generate bulk data for training the machine learning algorithm. As we continue into the next year of the research and better determine the scope of the machine learning algorithm integration with 3d food printing and predicting results, the scope and focus of data collection will be adjusted to demonstrate algorithm predictability for a subset of food printing cases while informing feasibility of the machine learning approach as a whole. We are continuing to follow protocols according to the Data Management Plan. What opportunities for training and professional development has the project provided?Professional development and training has been provided for 1 PhD graduate student and 1 MS graduate student at TTU. These students were trained in food ink preparation, 3D food printing processes, and relevant lab work for printing and characterizing 3D printed foods. How have the results been disseminated to communities of interest?Results were disseminated through an oral presentation to the engineering design community via the 2024 American Society of Mechanical Engineers International Design Engineering Technical Conference Design for the Manufacturing and the Lifecycle Conference Track through published paper with accompanying oral presentation. Results were disseminated through poster and oral presentation at the 2024 USDA NIFA AFRI Novel Foods and Innovative Manufacturing Technologies Project Director's meeting. Results were disseminated through a research poster presentation at the TTU Whitacre College of Engineering Research Day Research Day, 2024. What do you plan to do during the next reporting period to accomplish the goals?In the next reporting period research will focus on Specific Objective 2 to create and train a machine learning algorithm based on food ink characterization for protein mixtures and hydro-oleocolloids that are characterized for printability, texture analysis, and rheological properties. The machine learning will take inputs for food mixtures and mechanical properties of food and output expected printability based on training data. Further ingredients will food ink design will be conducted, especially to understand how food ingredients relate to rheological/textural properties, that then relate to printability. Multiple types of ingredient combinations will be explored to create a proof-of-concept machine learning algorithm to predict properties and/or printability from ingredient combinations that is informed by scientific experiments that characterize underlying food science and mechanics principles to interpret results.

Impacts
What was accomplished under these goals? Specific Objective 1 was planned for first year and Specific Objective 2 focused for second year, with accomplishments in the first year of the project accomplishing goals for each specific objective. For Specific Objective 1 Printability characterization for protein inks has been conducted for inks with potato mixed reinforced with pea protein and also hydrooleocolloids reinforced with whey protein. Our working hypothesis was investigated by altering the relative rations of ingredients (e.g. water, potato, and pea protein weight) and conducting texture analysis while also measuring dimensions of printed objects for each ink. Specific experiments conducted included systematically altering the relative amount of mashed potato, pea protein, and water to form protein inks with varied physical and sensory properties. Designs were generated to test overhang features for protein inks to assess their printability that can be used as input data to train the machine learning algorithm. Each protein ink was characterized for moisture content, pH, and textural properties. Specific experiments were also conducted to systematically alter ingredients for hydroolleocolloids by altering the amount of protein and wax in the system. The hydroollecolloids were printed to determine favorable ratios of ingredients to produce these novel foods and ensure consistent manufacturing. We successfully printed the hydroollecolloids which provides a positive result to continue the research into year 2 while investigating further ingredient combinations. For Specific Objective 2 Work has been conducted for planning the algorithm for machine learning and collecting preliminary data to link food ink ingredients, to mechanical properties, to printability that the machine learning algorithm will predict to personalize foods for users.

Publications

  • Type: Conference Papers and Presentations Status: Awaiting Publication Year Published: 2024 Citation: Khalil, I., F. Maleky, R. Pal, and P. Egan. Manufacturability of protein-reinforced foods with overhang designs. ASME IDETC Design for Manufacturing and the Lifecycle Conference. Washington, DC, 2024.(12 pages)
  • Type: Book Chapters Status: Submitted Year Published: 2025 Citation: Kahlil, M., A. Habib, Y. Kondabathula, O. Adebo, F. Maleky, and P. Egan. Multi-material printing for medical design. Food Additive Manufacturing: Materials, Process, and Products. Taylor and Francis Group, submitted.