Source: UNIV OF IDAHO submitted to NRP
PARTNERSHIP: INCREASING TROUT CONSUMPTION IN YOUNG CHILDREN AND FAMILIES FOR COGNITIVE AND MENTAL HEALTH BENEFIT
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1030176
Grant No.
2023-69015-39605
Cumulative Award Amt.
$1,115,500.00
Proposal No.
2022-08681
Multistate No.
(N/A)
Project Start Date
Aug 1, 2023
Project End Date
Jul 31, 2028
Grant Year
2023
Program Code
[A1344]- Diet, Nutrition and the Prevention of Chronic Disease
Recipient Organization
UNIV OF IDAHO
875 PERIMETER DRIVE
MOSCOW,ID 83844-9803
Performing Department
Family and Consumer Sciences
Non Technical Summary
Regular fish consumption may support brain health. Trout lines developed in Idaho containhigher levels of omega-3 fatty acids, nutrients important for human cognition and mentalwellbeing. Developed to support aquaculture sustainability, consumer preferences and humanhealth benefits of these fish are unknown. Addressing the program priority area Diet, Nutrition, and the Prevention of Chronic Diseases, the long-term goal of this integrated project is to utilizenutrition education strategies to increase adult and child consumption of fish to improve brainhealth as measured by cognitive and emotional wellbeing. Research objectives and activitiesinclude, (1) adult and child consumer panels to provide sensory evaluation on three strains oftrout, (2) effects of repeated exposure (RE) and child-centered nutrition phrases (CCNP) oneating behaviors and brain health will be determined using one control and two treatment groupsof children in childcare settings, (3) effects of nutrition education, incorporating CCNP and fish preparation techniques, and RE targeting family meals on eating behaviors of children and brainhealth of adults and children will be determined using four treatment groups in the home setting.Extension objectives and activities include developing and marketing About Trout! Pond toPlate, 12-video nutrition education series incorporating CCNP and fish preparation techniques.Education objectives and activities will provide students with experiential learningopportunities in curriculum development, evaluation, data collection, and analysis.Results from this project will provide foundational scientific evidence demonstrating successful approaches for facilitating adult and children's liking and consumption of fish to supportcognitive and mental health.
Animal Health Component
65%
Research Effort Categories
Basic
35%
Applied
65%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
7036010101050%
7043711309020%
8026099302030%
Goals / Objectives
The long-term goal of this integrated project is to utilize nutrition education strategies toincrease adult and child consumption of fish to improve brain health as measured by cognitive and emotional wellbeing.The research objectives of this project are to (1) describe the influence of trout strain (fish-mealdiet, CX line, CLX line) on the sensory properties and acceptance of trout, (2) determine how repeated exposure (RE) and repeated exposure plus child-centered nutrition phrases (RE+CCNP), in a childcare setting, will impact children's willingness to try, like, and consumetrout and the effect of trout consumption on cognitive and emotional wellbeing outcomes, and(3) determine how nutrition education, incorporating CCNP and fish preparation techniques, andRE targeting family meals in a home setting, will impact children's and adults' willingness totry, like, and consume trout and the effect of trout consumption on adults' and children'scognitive and emotional wellbeing outcomes. The extension objectives are to (1) develop atrout-centered nutrition education and recipe preparation 12-video series to be delivered through the Extension Foundation, and (2) develop and disseminate marketing materials to promote the 12-video series for use after this project ends. The education objectives are to provide graduatestudents with training and experiential learning opportunities in (1) curriculum development andevaluation and (2) data collection and analysis.
Project Methods
Research Objective 1:Two untrained consumer panels will provide sensory evaluation on three different strains of trout (fish-meal diet, CX line, CLX line).Children 3-5 years of age (n=50) will be recruited from preschools and childcare centers in the Pullman, WA/Moscow, ID area to determine acceptance of each trout sample. A second panel of adults (n=80) will be recruited from the same area to evaluate acceptance of each sample; questions regarding specific sensory properties will also be included to understand more about sensory properties and influence of these properties on liking. Each subject will be offered a 10 gram serving of each type of trout. Compusense sensory software will be used to present the sensory questionnaire, and collect all data.Research Objective 2:A within subjects, pre/post design will be employed to compare the effect of repeated exposure (RE) and repeated exposure plus child centered nutrition phrases (RE+CCNP) on eating behaviors of children 3-5 years of age. Children (n=99) will be recruited from the preschools and childcare centers in the Moscow, ID/Pullman, WA area. Children will be randomly assigned to a control, RE, or RE+CCNP group. Parents of all participating children will complete a demographic and trout consumption habits questionnaire. Children's baseline consumption and liking will be collected following established previously described protocols. The NIH Toolbox® for Assessment of Neurological and Behavioral Function (NIHTB) will be used to assess cognitive outcomes and emotional wellbeing measures. Children in the RE and RE+CCNP groups will be repeatedly offered trout, in which children will have 10 opportunities to taste a 2 ounce portion of CLX line trout and eat as much of the 2 oz and they would like. In the RE+CCNP condition, research assistants will also deliver the CCNPs (developmentally appropriate statements about the health benefits of trout, i.e. "trout helps your brain so you can learn and play"), while children taste the samples of trout to determine whether additional benefit in food behaviors occurs if messaging is paired with repeated exposure. In the RE condition, research assistants will only talk about the tasting procedure. Children in the control group will have 10 opportunities to interact with researchers participating in a non-tasting activity, such as a board game or coloring activity. Pre-post assessments will use standardized measures to assess children's willingness to try, liking of trout, and intake of trout (consumption of trout). Researchers will follow a previously developed protocol to capture children's state of hunger using "hunger dolls" to represent really hungry, really full, or not very hungry and not very full.After hunger is assessed, a hedonic rating scale will be used to assess children's taste preferences. Children will first be introduced to this system and understanding of the scale confirmed. The scale consists of three faces individually shown to the child, from left to right in the following order: yummy (smiling face), just okay (neutral face), and yucky (frowning face).Children will be presented with a 2oz portion of CLX trout, along with a water cup, and asked to try the trout. After trying the trout, children will be asked to place the portion cup in front of the face that describes how the trout tasted. They will then be allowed to continue eating as much of the trout as they would like. To determine increased intake, for the pre and post tasting activities the trout servings will be weighed pre/post consumption as a proxy of intake. At post assessment, cognitive and emotional wellbeing outcomes will again be measured using the NIHTB. Within and between group differences in cognitive and emotional wellbeing outcomes will be assessed using analysis of variance. Independent t-test will be used to examine the effects of repeated exposure on intake and liking of trout in children. When appropriate, parametric (e.g. multinomial regression, chi-square) and non-parametric tests will be used to evaluate parent responses from the questionnaire. Descriptive data will be reported for all outcomes.Research Objective 3:A 12-week quasi-experimental design will be employed to capture the translational impact of repeated exposure and nutrition education to the home setting on intake of whole CLX line trout and the effect on adult and youth cognitive and emotional wellbeing outcomes. A control and three treatment groups will be recruited from a convenience sample of Eat Smart Idaho participants in northern and south central Idaho. Eat Smart Idaho is a University of Idaho Extension program that delivers nutrition and physical activity programming to low-income Idahoans. Subjects (a parent and child aged 5-8 years from each family) will be recruited in cohorts of 6-7 dyads. These cohorts will be randomly assigned to one of four groups with 50 subjects (25 families) in each group. All groups will receive nutrition education from Eat Smart Idaho's standard 6-lesson in-person series. The control group (CTRL) will only receive this education. One treatment group (CCNP) will also be asked to view an online trout-specific nutrition education and recipe preparation video series, About Trout! Pond to Plate. A second treatment group (RE) will receive two servings of trout per week to prepare at home and consume. The third treatment group (RE+CCNP) will be asked to view the About Trout! Pond to Plate video series and receive two servings of trout per week. Dyads enrolled in the RE and RE+CCNP groups will receive a cooler with pre-portioned packages of flash frozen trout each week. They will be asked to prepare and consume 2 servings of fish each week for 12 weeks (adult servings is 4 oz. child serving is 2 oz. portion of CLX trout provided by the Hagerman Fish Culture Experiment Station). Data collected at baseline will include demographic information, a trout consumption habits questionnaire, child food preferences and consumption, and child and adult cognitive and emotional wellbeing outcomes (using NIHTB). After 6 weeks, child food preferences and consumption will again be assessed. After 12 weeks, the trout consumption habits questionnaire, and adult and child cognitive and emotional wellbeing outcomes will be assessed. Analysis of variance for the intake of trout and cognitive and emotional wellbeing outcomes will be used to examine the effects of the treatment conditions (RE, CCNP, RE+CCNP) to control in the home setting. When appropriate, parametric (e.g. multinomial regression, chi-square) and non-parametric tests will be used to examine parent responses from the questionnaire and changes in knowledge from the nutrition education lessons. Descriptive data will be reported for all outcomes.Extension Objective 1: A video series, About Trout! Pond to Plate, will be created to include lessons that center on preparing a child-friendly trout-centered mealwhere adults and children can follow along to prepare. The videos willinclude information on nutritional value of trout, food safety, and creating a positive mealtime environment,including the use of child-centered nutritional phrases. Alsoincluded in this 12-video series will be footage educating viewers on where trout come from,connecting them to the aquaculture industry in special "Pond to Plate" segments featuring thestudy team's aquaculture scientists.In order to evaluate the effectiveness of the curriculum, this series will include knowledge checks and self-reported behavior change incorporated into each lesson.Extension Objective 2: A multi-marketing strategy will be developed to promote the continued use of the video series including print and electronic flyersas well as a short video introducing the series to be shared on web-based platforms.Education Objectives 1 and 2:Fivegraduate students will contribute to accomplishing research and extension objectives.

Progress 08/01/24 to 07/31/25

Outputs
Target Audience:Research Objective 1: Children ages 3-6 years of age and adults 18 years of age or older, English speaking, residing in the Moscow/Pullman area Research Objective 2: Children age 4-6 years attending childcare in Moscow area Extension Objective 1: Children age 4-9 years of age and a parent/guardian residing in the Moscow, Idaho area Changes/Problems:The primary challenges this year were with recruitment for research objective 2. Recruitment was slower than we had hoped. Going into year 3 of the project, we have a group finishing the intervention and are actively recruiting about 14 more subjects to meet our sample size. For extension objective 1, creating the Pond to Plate curriculum, we are slightly behind schedule, but are receiving assistance from UI Extension Publishing to help us get back on track. What opportunities for training and professional development has the project provided?The primary opportunities for training and professional development were in the form of student training as described above under the education objectives. In addition, all graduate students working on the project were given the opportunity to submit abstracts for professional conferences. Three students were provided the opportunity to present poster presentations. How have the results been disseminated to communities of interest?Data collection continues to be ongoing. Preliminary results were presented at professional conferences. What do you plan to do during the next reporting period to accomplish the goals?Research objective 1: Data analysis will be completed and a manuscript drafted. Research objective 2: Data collection and analysis will be completed Research objective 3: Protocols will be revised to accomodate progress on extension objective 1 (the curriculum will be used in research objective 3). Recruitment and data collection will begin. Extension Objective 1: We will work with the University of Idaho Extension Publishing graphic designer to finalize the curriculum. Curriculum will be translated to Spanish. Extension Objective 2: Marketing materials will be drafted and revised based on feedback from the priority population Education Objectives: Three PhD students will continue to work on the research and extension objectives. Undergraduate and graduate students will be given opportunities to assist with active data collection during the academic year and summer 2026.

Impacts
What was accomplished under these goals? Research Objective 1: A sensory study was conducted to understand acceptance of trout in children (aged 3-6 years old; n=50), identify the relationship between acceptance and at-home feeding experiences, and assess liking of three trout strains.An adult panel (n=102) was conducted in late spring and results are being analyzed summer 2025.There were no differences in children's liking among the strains (p>0.05).Children who struggled with eating lumpy textured foods at home were more likely to dislike trout. Children with more exposure to fish at home were more likely to rate the fish as yummy. Research Objective 2:Children (aged 4-6 years old; n=76) were enrolled in the 12-week repeated exposure study. Data collection and enrollment is still in progress. Preliminary results (n=52) on liking, intake, and emotional wellbeing were analyzed for student thesis projects.Preliminary data analysis indicates participation in a 12-week intervention study increases children's liking of trout.Preliminary data analysis indicates most children (76%) ate a weekly average of ≤ 0.5 oz of trout offered.Relationship to emotional wellbeing cannot yet be determined. Data collection is ongoing. Extension Objective 1:Storyboards for About Trout! Pond to Plate were completed and reviewed. Five expert reviewers rated modules on scientific accuracy, content organization, learning outcomes and provided feedback. The interdisciplinary panel was comprised of one Aquaculture expert, one Food and Nutrition expert, two Child and Family Life Development experts and one Food Security, Extension, Family and Consumer Sciences expert.Experts rated each module's learning objectives and content on a four-point scale (1 = strongly disagree to 4 = strongly agree). Ratings were used to calculate a content validity index (CVI) score. Scale-level CVI/Average (S-CVI/Ave), and Scale-level CVI/Universal Agreement (S-CVI/UA) scores are shown here. A score of ≥0.80 indicated strong content validity, while lower scores identified areas needing revision.Overall course review score indicated no significant revisions needed (CVI ≥0.80). Seven individual modules required no revisions (CVI ≥0.80), while the other 5 are being revised based on reviewer comments and feedback. The only significant edit will be to add on-farm interviews to module 6, DIY Pond to Plate. These interviews were filled in late spring 2025. EducationObjectives: Two students defended the theses in M.S. Nutritional Sciences. One M.S. Nutritional Sciences student converted to Ph.D. Nutritional Sciences and is continuing to work on Extension Objectives. One new Ph.D. student in Nutritional Sciences was recruited to start in the fall 2025. One Ph.D. student in Food Science continues to support research objective 1. Three student interns gain experience with data collection, assisting with reserach objective 2.

Publications

  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2025 Citation: Davis, J., Roe, A., Johnson, S., Howard, T., Powell, M., Kendall, J., Kindelspire, H., Gardiner, J., Ross, C., Lee, S.G., Bledsoe, J. (2025). Effect of trout on emotional well-being in children aged 4-6 years. Journal of Nutrition Education and Behavior, Volume 57, Issue 8, S7. Presented at Society of Nutrition Education and Behavior Conference, Indianapolis, IN.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2025 Citation: Kindelspire, H., Roe, A., Johnson, S., Lee, S.G., Kendall, J., Bledsoe, J., Ross, C., Powell, M. (2025). Online curriculum development and validation for parent-focused nutrition education program About Trout! Pond to Plate. Journal of Nutrition Education and Behavior, Volume 57, Issue 8, S60 - S61. Presented at Society of Nutrition Education and Behavior Conference, Indianapolis, IN.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2025 Citation: Kendall, J., Roe, A., Davis, J., Bledsoe, J., Johnson, S., Gardiner, J., Powell, M., Kindelspire, H., Ross, C., Lee, S.G., Tsao, L., Kaur, H. (2025). Repeated exposure and child centered nutrition phrases impact on the liking of trout in children aged 4-6 years. Journal of Nutrition Education and Behavior, Volume 57, Issue 8, S77. Presented at Society of Nutrition Education and Behavior Conference, Indianapolis, IN.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2025 Citation: Roe, A. Ross, C., Johnson, S., Lee, S.G., Bledsoe, J., Powell, M., Gardiner, J., Davis, J., Kendall, J., Kindelspire, H., Potter, R. (2025). Increasing trout consumption in young children and families for cognitive and mental health benefit: year two. Journal of Nutrition Education and Behavior, Volume 57, Issue 8, S86. Presented at Society of Nutrition Education and Behavior Conference, Indianapolis, IN


Progress 08/01/23 to 07/31/24

Outputs
Target Audience:Research Objective 1: Children age 3-6 years of age, English speaking, residing in the Moscow/Pullman area Research Objective 2: Children age 4-6 years of age attending childcare in Moscow and Potlatch, Idaho Extension Objective 1: Children age 4-9 years of age and a parent/guardian residing in the Moscow, Idaho area Changes/Problems:The major challenge faced this year was recruiting subjects for research objective 2. Due to the long intervention time (12 weeks), this is challenging working around school breaks and summer vacations. We will need to extend data collection to spring 2025 and possibly summer 2025 for this objective. However, we have strong childcare site partnerships and a great team of student researchers to be able to accomplish this objective. What opportunities for training and professional development has the project provided?The primary opportunities for training and professional devleopment were in the form of student training. Two PhD students, 1 MS student, and 1 high school student were trained on the sensory evaluation protocol and assisted with data collection for research objective 1. Three MS students and 4 summer interns were trained on the repeated exposure protocol and assisted with recruitment, data collection, and data entry for research objective 2. Two staff members (hatchery manager and research technician) and a summer undergraduate intern were given the opportunity to contribute to the videos for extension objective 1. This provided an opportunity to develop skills for presenting to public. Professional development opportunities were provided to key personnel through working with Extension Publishing to identify best practices and peer-review methodology for extension objective 1. How have the results been disseminated to communities of interest?In this first year, there are not many results to share. Updates on the project were provided to peers at the Society of Nutrition Education and Behavior conference through a poster presentation. What do you plan to do during the next reporting period to accomplish the goals?Research objective 1: Data collection will be completed and analyzed, results will be presented at a national conference, manuscript will be drafted. Research objective 2: Data collection will be completed and analyzed, results will be presented at a national conference, manuscripts will be drafted. Research objective 3: IRB protocols and data collection forms will be finalized Extension objective 1: Curriculum will undergo expert review to establish content validity and pilot testing to establish face validity; results will be presented at a national conference, manuscript will be drafted Extension objective 2: marketing materials will be drafted, feedback obtained from target audience, and revised Education Objectives: Three students will complete and defend their theses in Nutritional Sciences, additional students will be recruited to join the project in year 3

Impacts
What was accomplished under these goals? In year one, research activities focused on protocol finalization and researcher training. Across institution Institutional Review Board reliance agreements and data use agreements were approved. Recruitment sites were identified and prioritized. Subject recruitment and data collection to began in May 2024 for research objective 2 (18 enrolled) and in July 2024 for research objective 1 (51 children completed). Extension activities included progress on curriculum content development and storyboard creation as well as working with University of Idaho Extension publishing to identify the appropriate online platform and timeline for peer review. Recipes were tested in May/June and videos of all recipes included in the curriculum were filmed in summer 2024. Education activities included recruiting three graduate students to assist with research objective 2 and extension objective 1. All three students successfully defended their thesis proposals, which will focus on different aspects of the larger grant. A fourth graduate student is assisting with research objective 1, a portion of which may be included in the student's dissertation. An additional PhD student, MS student, and 1 high school student also assisted with data collection on research objective 1. A research specialist was also hired to assist with overall project management and four summer interns were hired to assist with data collection and videos. We held a successful and very productive advisory board meeting in January 2024

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Roe, A., Powell, M., Ross, C., Johnson, S., Lee, S. G., & Bledsoe, J. (2024). Increasing Trout Consumption in Young Children and Families for Cognitive and Mental Health Benefit: Year One. Journal of Nutrition Education and Behavior, 56(8), S94S94. https://doi.org/10.1016/j.jneb.2024.05.210