Source: PURDUE UNIVERSITY submitted to
NOVEL NATIVE STARCH GRANULE-GUEST INCLUSION COMPLEXES: FORMATION, STRUCTURE, AND FUNCTION
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1030160
Grant No.
2023-67017-40249
Cumulative Award Amt.
$598,000.00
Proposal No.
2022-09190
Multistate No.
(N/A)
Project Start Date
Jul 1, 2023
Project End Date
Jun 30, 2026
Grant Year
2023
Program Code
[A1364]- Novel Foods and Innovative Manufacturing Technologies
Recipient Organization
PURDUE UNIVERSITY
(N/A)
WEST LAFAYETTE,IN 47907
Performing Department
(N/A)
Non Technical Summary
High quality and nutritious foods are essential for human health and well-being. The starch industry is central to food production and economies world-wide. Starch functionality is intricately related to the complex structure of the starch granule, which creates opportunities for modifying starch properties to address important societal issues. We propose a novel approach to modify starch functionality by incorporating small guest molecules with advantageous nutritional traits into the starch granule amorphous regions. Our long-term goal is to create novel starch granule-guest inclusion complexes with desirable structure-function traits for food quality and nutritional value while advancing fundamental understanding of the basis for guest molecule interactions with starch. These value-added novel starch ingredients have potential economic benefit to numerous industries, and the guest molecules will increase dietary fiber, antioxidant, and amino acid profiles in foods while improving the starch functionality in reduced-sugar formulations. Our objectives are to: 1) create starch granule-guest inclusion complexes, 2) understand the thermodynamic and structural basis of guest molecule interactions within starch granules, 3) document effects of the guest molecules on starch structure and function, and 4) develop recommendations for food products that would benefit from using these novel ingredients. This work addresses program area priorities "develop novel ingredients to ensure the quality and nutrition of foods; improve understanding of the chemical and physical properties of novel ingredients" in Novel Foods and Innovative Manufacturing Technologies A1364.
Animal Health Component
25%
Research Effort Categories
Basic
75%
Applied
25%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
50250102000100%
Knowledge Area
502 - New and Improved Food Products;

Subject Of Investigation
5010 - Food;

Field Of Science
2000 - Chemistry;
Goals / Objectives
The long-term goal of this project is to create novel starch granule-guest inclusion complexes with desirable structure-function traits for food quality, nutritional value, and shelf-life applications while advancing fundamental understanding of the basis for guest molecule interactions with starch.The specific objectives of this proposal are to:Create starch granule-guest molecule inclusion complexes.Understand the thermodynamic and structural basis of guest molecule interactions within the starch granules.Document the effects of the guest molecules on the structure and function of the starch, as well as the stability of these novel ingredients.Develop recommendations for food products that would benefit from using these ingredients in the formulations for quality, nutrition, and shelf-life improvement.
Project Methods
The solid state properties of the samples will be documented by a number of methods, including: X-ray diffraction (XRD) for crystal identification and phase analysis; DSC to determine glass transition, melting, and crystallization temperatures (Tg, Tm, Tcry), thermogravimetric analysis (TGA) to verify the Tm or degradation temperature and moisture content; controlled temperature and RH polarized light microscopy; moisture sorption profiling to document susceptibility to moisture uptake and the equilibrium moisture content at a given storage RH; moisture content using Karl Fischer titrations and/or vacuum oven drying; solubility determination; and dissolution rate by intrinsic dissolution studies, with HPLC or refractive index analysis of the amount dissolved as a function of time. The viscosities of the guest molecule solutions will be measured using capillary viscometers and RVA methods, and the aw and pH values will be determined at different solids concentrations. Guest molecule partitioning into starch granules will be evaluated by monitoring the loss in solution concentration of the guest molecule from bulk aqueous phase following equilibration with starch solutions.We will compile and compare all results to document the extent/magnitude of structural and functional changes across the different guest molecule types and treatments, and the functional traits will be mapped to desirable Tgel and RVA profiles for a variety of products. The starch-guest complexes with the highest Tgels in water will be further studied in 'model' solution and product formulations. Initial results will be analyzed by linear regression, Pearson's correlation coefficients, and ANOVA/MANOVA/mixed linear models followed by a Tukey's HSD post hoc test (α=0.05) to test the significance of the main factors and individual differences in means. Then principal component analysis will be used to elucidate structure-activity relationships between the main experimental variables (starch type, guest type, etc.), key molecular properties of the guest molecule form, formulation, and responses (Tgel, crystallinity, RVA parameters, digestibility, other functions). We expect the novel starch-guest ingredients can be used to improve the quality, shelf-life, and nutrient profile of foods; that the starch-guest complexes with the highest Tgels can be used in formulations that require less sucrose to produce the desirable starch-based structure; and that the amounts of internalized guest molecules (and their influence on starch structure and resistance to digestion) will be enough to significantly increase the amount of dietary fiber/antioxidant/amino acids in foods when used at common starch amounts in products.

Progress 07/01/23 to 06/30/24

Outputs
Target Audience:The target audience for this work is the food industry, including member companies of the Whistler Center for Carbohydrate Research, other scholars working in this area, disciplinary journals, and ultimately the consumer, all of whom we anticipate benefitting from the opportunity for strategically formulating foods using the modified starches and formulation strategies being developed in this work. This first year of the project reached member companies fo the Whistler Center for Carbohydrate Research.. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This first year of the project was a buidling year, with transfer of knowledge from a current graduate student completing his degree to a newly hired graduate student whose work will be dedicated to this project. The students learned the techniques to be used initially to address objectives 1 and 3 of the project, and engaged with the Whistler Center for Carbohydrate Research member companies during campus events (meetings, poster session). How have the results been disseminated to communities of interest?In this first year, engagementand dissemination has been limited to presentations and discussions on campus and with Whistler Center for Carbohydrate Research member companies. Plans for upcoming years as the project progresses will spread outward, including publication, presentation, and application for patent. What do you plan to do during the next reporting period to accomplish the goals?A second graduate student will be hired to build the team, which will then expand work from objectives 1 and 3 on to objective 2 and then objective 4. Year 2 of the project will primarily focus on the first 3 project objectives. Efforts will focus primarily on wheat and corn starches, with some inclusion of tapioca starch, expanding from examination of functional differences to also include structural analyses. Effects of different classes of guest molecules (sugars vs. salts) will be investigated as part of objective 2, understanding the thermodynamics of the interactions and resulting effects on starch structure and functionality. These studies will be written up as manuscripts and submitted for publication when ready. A patent application will also be pursued.

Impacts
What was accomplished under these goals? This first year of the project was a buidling year, with transfer of knowledge from a current graduate student completing his degree to a newly hired graduate student whose work will be dedicated to this project. Together, they made good progress on objectives 1 and 3, and related publications are in the pipeline. A second graduate student will be hired in year 2 of the project to further efforts to address all 4 objectives. For objectives 1 and 3, the students focused first on investigating the effects of a variety of small guest molecules (sugars, oligosaccharides, salts, vitamins)on wheat starch, exploring guest molecule type and concentration effects on the functionality of the wheat starch in the solutions of the guest molecules, followed by preconditioning the wheat starch in the guest molecule solutions in different temperature environments for different lengths of time (hydrothermal treatments) to create the starch-guest inclusion complexes (physically modified starch). These modified starches were then subjected to the same differential scanning calorimetry and rapid visco analysis methods to determine how the hydrothermal treatments affected the starch functionality. Initial studies were then carried out using other starch types: tapioca and several varieties of corn starch (dent, waxy, high amylose) to compare how the different starch types responded to the guest molecule hydrothermal treatments. Results indicate that the type and concentration of the guest molecule, as well as the conditions used in hydrothermal treatment, all affect starch functionality, with small differences in gelatinization temperature and wide ranges in swelling and pasting behaviors. These differences in functionality indicate that the starch granule-guest inclusion complexes (physically modified starch in which the native granule structure contains internalized guest molecules) have promising applications in a variety of food products - the type of complex could be strategically selected for the desired starch functionality in the product under consideration (cake, cookie, sauce, etc.).

Publications