Source: IOWA STATE UNIVERSITY submitted to NRP
TESTING AND RELEASE OF FORAGE SORGHUM CULTIVARS ADAPTED TO UPPER MIDWEST STATES
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1030092
Grant No.
2023-67013-39304
Cumulative Award Amt.
$498,961.00
Proposal No.
2022-10309
Multistate No.
(N/A)
Project Start Date
Apr 1, 2023
Project End Date
Mar 31, 2027
Grant Year
2023
Program Code
[A1143]- Conventional Plant Breeding for Cultivar Development
Recipient Organization
IOWA STATE UNIVERSITY
2229 Lincoln Way
AMES,IA 50011
Performing Department
(N/A)
Non Technical Summary
Sorghum is considered the most productive and versatile annual crop for forage use with numerous advantages related to management and sustainability. Due to its high biomass productivity potential, lower cost of production and the capacity to provide quality forage for both beef and dairy cattle operations, sorghum has emerged as a sustainable option for many livestock producers. However, the expansion of sorghum cultivation to the northern U.S. is limited mostly due to shorter growing seasons and low temperatures at planting time. Corn silage and alfalfa or grass hay are predominantly used in the Upper Midwest U.S., despite sorghum's economic advantages and environmental sustainability benefits. While the proposed research does not aim to completely replace corn silage or hay with forage sorghum, it is designed to offer an alternative by developing improved sorghum germplasm with high biomass yield and nutritive value that could be produced instead of other forages in northern regions with sandy soils and low organic matter, and/or drought prone areas in the target states. In addition to the value of sorghum for silage in northern states, this crop offers a broad array of plant types and growth patterns that can be leveraged for diverse animal farm operations. Hay, green chopping, direct grazing, or silage are all possible harvesting and feeding practices that can be performed with the adequate sorghum type. Sudangrass sorghums will provide high biomass yield with good quality that can be used for grazing, green chopping, or hay, due to its thin stems, high density leaves, and high regrowing capacity. Sorghum-sudangrass hybrids with photoperiod sensitivity can produce high biomass tonnage during a wider window of time, offering farmers flexibility for their animal operations. Extension guidelines have been developed in the target regions to make recommendations about planting and harvesting times, plant density, fertilizer rates, and harvest management practices (Lang, 2001; Sedivec et al., 2020). This versatility in management and feeding practices needs to be accompanied by superior genetics for the target states, which will be identified in this project and made available to stakeholders.The Iowa State University (ISU) sorghum breeding program is the northernmost public program in the U.S. dedicated to the development of parental lines for the production of forage hybrids with adaptation to shorter growing seasons. The goal of the proposed project is to test and release new ISU sorghum cultivars adapted to northern latitudes to provide high-yielding high-nutritive value forage to beef and dairy cattle operations in the Upper Midwest U.S. Advanced experimental hybrids for alternative systems (hay, green chopping, grazing, and silage) will be evaluated in regional trials comprising eight locations in Iowa, North Dakota, South Dakota and Wisconsin. Additionally, management treatments relevant to the target environments will be evaluated to select the best performing hybrids for those production systems. This project will identify high-yielding sorghum parental lines and hybrid combinations with the best adaptation to Iowa, Wisconsin, North Dakota and South Dakota, based on biomass yield and composition for diverse livestock operations. Alternative management systems for specific regions will be identified to improve the profitability and sustainability of their farming operations and associated communities. Finally, the new sorghum germplasm will be released for public use benefiting not only beef and dairy producers in northern states but also numerous small and mid-size seed companies that do not have proprietary sorghum germplasm to supply the best performing forage sorghum cultivars to their clients in these northern regions.The proposed research will have a significant impact on end users of the sorghum germplasm and local communities because: (a) Beef and dairy cattle operations are a critical component of the economy of our target states. (b) Forage sorghum nutritive value can be improved through breeding to make it comparable with corn for silage. (c) The sorghum seed market is moderately concentrated with several mid-size or small companies testing germplasm in a limited region and/or with no proprietary genetics. (d) Sorghum breeding programs (both public and private) generally prioritize the development of germplasm adapted to the Southern Great Plains but not the Upper Midwest U.S.
Animal Health Component
50%
Research Effort Categories
Basic
(N/A)
Applied
50%
Developmental
50%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20216301081100%
Knowledge Area
202 - Plant Genetic Resources;

Subject Of Investigation
1630 - Summer annual grasses;

Field Of Science
1081 - Breeding;
Goals / Objectives
The Iowa State University (ISU) sorghum breeding program is the northernmost public program in the U.S. dedicated to the development of parental lines for the production of forage hybrids with adaptation to shorter growing seasons. The goal of the proposed project is to test and release new ISU sorghum cultivars adapted to northern latitudes to provide high-yielding high-nutritive value forage to beef and dairy cattle operations in the Upper Midwest U.S. Advanced experimental hybrids for alternative systems (hay, green chopping, grazing, and silage) will be evaluated in regional trials comprising eight locations in Iowa, North Dakota, South Dakota and Wisconsin.Additionally, management treatments relevant to the target environments will be evaluated to select the best performing hybrids for those production systems.The following specific objectives will be accomplished:1) Objective 1: Identify high-yielding sorghum parental lines and hybrid combinations adapted to Iowa, North Dakota, Wisconsin, and South Dakota.2) Objective 2: Identify superior forage sorghum hybrids for specific market needs.Sub-Obj. 2A. Determine best performing hybrids based on biomass composition analysis for beef and dairy cattle productions systems.Sub-Obj. 2B. Evaluate experimental hybrids using alternative management systems for specific regions to improve farmers' profitability and environmental sustainability.3) Objective 3: Release of superior parental lines identified in Obj. 1 and 2.
Project Methods
The testing and release of forage sorghum cultivars adapted to Upper Midwest states will be accomplished by the following as follows:a) For Objective 1: Identify high-yielding sorghum parental lines and hybrid combinations adapted to Iowa, North Dakota, Wisconsin, and South Dakota.The seed of 20-25 ISU elite experimental sorghum hybrids will be generated every year in the ISU breeding program. Hybrid seed will be produced by planting parental lines in isolation plots in Iowa but could be complemented by hand-pollinations and/or winter nursery seed production, if insufficient amounts are obtained from on-season isolation plots. Hybrid seeds will be manually harvested, counted/weighted, and treated with safener (Concept and fungicides) for the application of pre-emergent herbicides. Approx. 12,000 seeds of each hybrid will be produced yearly, including silage type hybrids, sudangrass, and sorghum-sudangrass hybrids. Some materials will be BMR and/or photoperiod sensitive.Testing trials will be established in IA, WI, ND, and SD to identify the best performing elite experimental hybrids with biomass yields superior to commercial materials in each location and within each sorghum type (forage for silage, sudangrass and sorghum-sudangrass). Experimental hybrids and commercial checks will be planted in 4-row plots, with plot length and row spacing depending on the location, following common practices for that state and technical capabilities of each participating institution. Experiments will be conducted in eight locations as follows: (1) Boone, IA: 42.0597° N, 93.8802° W.; (2) Greenfield, IA: 41.3053° N, 94.4614° W; (3) Crawfordsville, IA: 41.2140° N, 91.5387° W; (4) Fargo, ND: 46.8772° N, 96.7898° W; (5) Beresford, SD: 43.0805° N, 96.7737° W; (6) Rapid City, SD: 44.0805° N, 103.2310° W; (7) Hancock, WI: 44.1336° N, 89.5232° W; (8) Marshfield, WI: 44.6689° N, 90.1718° W. These locations vary in climatic conditions, including rainfall, GDD, days of first and last killing frost, etc.b) Objective 2: Identify superior forage sorghum hybrids for specific market needs.2A. Determine best performing hybrids based on biomass composition analysis for beef and dairy cattle production systems.Biomass samples will be ground and analyzed for forage nutritive value: ash, crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and neutral detergent fiber (NDFD-48 hours) using an XDS Near Infrared Rapid Content Analyzer. As needed, a few samples will be sent for wet chemistry to the NDSU Animal Sciences lab to adjust the calibrations. Composition data will be analyzed as described for yield data.2B. Evaluate experimental hybrids using alternative management systems for specific regions to improve farmers' profitability and environmental sustainability.The experiment will evaluate the three hybrid types harvested in a two-cut or one-cut system and with and without intercropping with alfalfa. The best hybrids for each region selected according to performance in the variety trial in Years 1 and 2 will be planted in a RCBD with a factorial arrangement and four replicates. Treatments will include three sorghum types (sorghum-sudangrass, sudangrass and a silage type), two-cut systems (one or two cuts) and with or without intercropping with alfalfa (17 kg pure live seed/ha). Sorghum managed in a two-cut system will be seeded at a higher rate (450,000 pl/ha; 17 kg/ha), similar to current industry practices.Evaluations for this experiment will be as described for Objective 1, including the forage nutritive value. Normalized difference vegetation index (NDVI) will be measured bi-weekly using a handheld GreenSeeker and a UAV (in IA and ND) after the cash crop reaches ~V8-V10 stage to correlate both forage and grain yield. Alfalfa stem and plant counts will be measured at each harvest and in the following spring to evaluate alfalfa establishment and over-wintering. At harvest, sorghum and alfalfa plants from a subsample area will also be separated and dried to determine the proportion of each forage in the plots. The best ISU germplasm for the proposed alternative management practices will be identified following analyses described for Obj. 1 and 2A.c) Objective 3: Release of superior parental lines identified in Obj. 1 and 2.The germplasm to be released as part of this project will be the parental lines (pollen or seed inbred lines) used to generate the tested experimental hybrids. Breeder seed of superior parental lines will be generated at the end of Year 3 of the project, after identification of superior hybrids based on performance in IA, SD, ND, and WI. New sorghum germplasm will be reported to the ISU Office of Intellectual Property and Technology Transfer (OIPTT) as a Record of Invention. All information relating to new germplasm is summarized and documented in the "Intellectual Property Disclosure and Record" (IPDR) form in addition to pedigree, any unique characteristics that justify the release and any existing contractual obligations. Foundation seed of released lines will be produced by the Committee for Agricultural Development (CAD). Information and characteristics of the superior sorghum inbred lines will be published in the Journal of Plant Registration. A sample of seed of the parental inbred lines will be deposited in the USDA-ARS National Laboratory for Genetic Resources Preservation (NLGRP) prior to publication.

Progress 04/01/23 to 03/31/24

Outputs
Target Audience:The following audiences were the targets during this reporting period: 1. Farmers in the Upper Midwest U.S.: The new sorghum forage sorghums adapted to northern latitudesbenefit beef and dairy cattle producers in Iowa, North Dakota, South Dakota and Wisconsin. The benefits will include: (i) increased profitability due to higher biomass yields of sorghum hybrids selected as part of this project and lower production costs than other annual forages, including corn; (ii) improved flexibility in farmers' operation due to the sorghum versatility adapting to different types of operations (green chopping, direct grazing, silage, and hay). 2. Local communities: Beef and dairy cattle operations are a significant component of the economy of our target states. South Dakota, Iowa and Wisconsin are ranked 7th, 8th, and 9th, respectively, as leading states on livestock production, while North Dakota is ranked 9th in the number of beef cows with calves. Wisconsin is ranked second in milk production, which represents 14% of the total U.S. production. These prevalent production systems are reflected in the extent of corn grown for silage in these regions. While the proposed research does not aim to completely replace corn silage or hay with forage sorghum, it will benefit local communities by offering an alternative crop with superior genetics (improved sorghum germplasm with high biomass yield and nutritive value) that could be produced with sustainable management practices instead of other forages in northern regions with sandy soils and low organic matter, and/or drought prone areas in the target states. 3. Seed companies: Public breeding programs to improve sorghum for forage use can have a significant impact on the seed industry providing superior genetics to farmers in northern regions because: (i) the sorghum seed market is moderately concentrated with only one large company actively breeding for forage use and several mid-size or small companies testing germplasm in a limited region or with no proprietary genetics; and (ii) companies with breeding programs for forage use are located in the southern Great Plains, evaluating and selecting germplasm adapted to dry and hot growing conditions and thus, not optimized for the Upper Midwest U.S. The Iowa State University (ISU) sorghum breeding program is the northernmost public program in the U.S. (42.0308° N), dedicated to the development of parental lines for the production of forage sorghum hybrids, with adaptation to shorter growing seasons. The ISU germplasm released as a product of this project could be licensed by seed companies in a non-exclusive manner to benefit their clients in the target regions. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project has provided professional development opportunities for one technician, three undergraduate students in Agronomy-related majors, and one PhD student in the form of one-on-one work with a mentor/supervisor. How have the results been disseminated to communities of interest?The following extension and outreach activities were conducted in this reporting period to disseminate goals and results to communities of interest: Forage Field Day, Beresford, SD Research Farm August 7, 2023; plot tour and talks (80 attendees- predominantly farmers). Southeast Research Farm Fall Field Day, Sept. 14, 2023; plot tour and talk (35 attendees- farmer audience). NACAA Am/Pic Conference, Des Moines, IA Aug. 14-17, 2023; connections made/many discussions on research (Extension professionals). Northern Plains Forage Association Annual Meeting, Brandon, SD Dec. 1, 2023; connections made/discussions with producers and other agronomists. Midwest Forage Association WI Symposium, WI Dells, WI, Feb. 20-21, 2024; connections made/ discussions with other Extension personnel. Co-PI from ND attended the American Forage Grassland Council meeting on January 8-10 2024. She discussed this project with several attendees, and her student presented the need for forage sorghum varieties in cold climates as part of an oral presentation. Omeye, F. M.T. Berti, H. Mosqueda, A. Kurth, H. Lindell. And Md S. Islam. 2024. Increased Forage Yield and Nutritive Value of Forage Sorghum Grown in Intercropping with Alfalfa. American Forage Grassland Conference, Mobile AL January 8-10, 2024. Field day in Fargo, ND September 19, 2024, Attendance 60 people. We showed the sorghum variety trial at the field day. What do you plan to do during the next reporting period to accomplish the goals?During the 2024 growing season, a larger number of experimental hybrids will be evaluated in the target states(2 locations in SD, 3 environmentsin IA, 1 location in ND and 2 sites in WI). These hybrids include dual purpose, forage, multi-cut and photoperiod sensitive types. Phenology, biomass yield and composition data will be obtained to make selections for advancement and future management studies to be conducted in years 3 and 4.

Impacts
What was accomplished under these goals? Due to its high productivity potential, lower cost of production and the capacity to provide quality forage for both beef and dairy cattle operations, sorghum has emerged as a sustainable option for many livestock producers. However, the expansion of sorghum cultivation to the northern U.S. is limited mostly due to shorter growing seasons and low temperatures at planting time. The Iowa State University (ISU) sorghum breeding program is the northernmost public program in the U.S. dedicated to the development of parental lines for the production of forage hybrids with adaptation to shorter growing seasons. The goal of the proposed project is to test and release new ISU sorghum cultivars adapted to northern latitudes to provide high-yielding high-nutritive value forage to beef and dairy cattle operations in the Upper Midwest U.S. Advanced experimental hybrids for alternative systems (hay, green chopping, grazing, and silage) will be evaluated in regional trials comprising eight locations in Iowa, North Dakota, South Dakota and Wisconsin. Beef and dairy cattle operations are a critical component of the economy of our target states. This project will induce a change in action and conditions of our stakeholders (mostly farmers and the seed industry serving the Upper Midwest US). Farmers will be able to adopt the use of high-yielding high-nutritive value sorghum forage hybrids developed specifically with adaptation to their production region. These sorghums will be produced as animal feed at a lower cost than corn, with similar yield and quality, while improving the sustainability of the farming system through rotations and stable yields even under drought. The seed industry serving these northern states are mid- or small-size companies with no proprietary genetics, and thus, their business is affected by the lack of superior sorghum germplasm that is suitable for their clients. This project will generate germplasm that could be leveraged by these companies, improving the development of the seed business in the region, with a positive impact on clients (farmers) but also the work force they employed. Objective 1: Identify high-yielding sorghum parental lines and hybrid combinations adapted to Iowa, North Dakota, Wisconsin, and South Dakota. Yield testing trials were established for the 2023 growing season in the following locations: Iowa: Boone, Crawfordsville, Lewis. North Dakota: Fargo South Dakota: Beresford, Pierre, Hayes. Wisconsin: Hancock. Advanced experimental hybrids from the ISU sorghum breeding program were tested in these locations including: five photoperiod sensitive hybrids, two forage types for single cut at the end of the season, three forage types for multiple cuts throughout the growing season, two dual purpose types and three grain types (tested in South Dakota only). Commercial checks for all types were also included. Stand counts, plant height, flowering time, and lodging scores were recorded. Biomass (wet and dry weights) were obtained at the end of the season or at multiple time points, according to hybrid type. There was significant variation in biomass yield, particularly within forage and photoperiod sensitive types, with mean values of 10.7 MT dry matter/ha for dual purpose types, 14.8 MT dry matter /ha for forage types and 15.9 MT dry matter/ha for photoperiod sensitive hybrids. Plant height was, as expected according to classification, with shorter dual purpose hybrids (mean=2.05m), followed by forage hybrids (mean=2.9m) and photoperiod sensitive sorghums (mean=3m). Quantitative traits were analyzed using a mixed model with location, replication nested within location, and genotype by location interaction as random effects, genotype as fixed effect and stand counts as covariate. Several experimental hybrids yielded as well as commercial hybrids (not statistically significantly different than commercial check for a particular hybrid type). These hybrids will be re-tested, together with new advanced experimental hybrids, during the 2024 growing season. Objective 2: Identify superior forage sorghum hybrids for specific market needs. 2A. Determine best performing hybrids based on biomass composition analysis for beef and dairy cattle production systems. Biomass subsamples for all hybrid types and locations listed in Objective 1 were sent to North Dakota State University (NDSU) to determine biomass composition using Near Infrared Reflectance Spectroscopy (NIRS). Forage nutritive value can be determined from multiple parameters which were estimated using NIRS and derived formulas, including: protein, acid detergent fiber (ADF), neutral detergent fiber (NDF), fat, lignin, ash, total digestible nutrients (TDN), dry matter intake (DMI), and relative feed quality (RFQ). Additionally, a total of 139 samples from 29 new experimental hybrids grown in three locations in Iowa were analyzed using NIRS by NDSU. This analysis was conducted to identify new advanced experimental hybrids with diverse and superior composition that could enrich the portfolio to be tested in ND, SD and WI in 2024-2026 seasons. The following hybrids were identified as superior based on composition: (i) for dual purpose types: HDP00121; (ii) for forage types: HF55151 (high DMI, low lignin and high yield), and HF5591 (high DMI, low lignin and intermediate yield); and (iii) for PS types: a new female parental line was identified that improved composition in hybrids with IA100RPS as pollen parent. These hybrids, among others, will be planted in the 2024 growing season in all locationsto evaluate yield and composition in the target states. 2B. Evaluate experimental hybrids using alternative management systems for specific regions to improve farmers' profitability and environmental sustainability. Activities related to this objective will be conducted in the last 2 years of the project. Objective 3: Release of superior parental lines identified in Obj. 1 and 2. Activities related to this objective will be conducted in the last year of the project.

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