Progress 02/15/23 to 02/14/24
Outputs
Target Audience:The target audience for our work and products arefood and beverage processors but in particularmeat and poultry processors. More broadly our audienceincludes any industry that processes biological materials and is required to clean and sanitize equipment.This work is specifically targeted to small and mid-sized meat and poultry processors, but the work can be adapted to lateral markets in food processing wherever chemical and microbial contamination of products is a concern. We aim to produce non-toxic natural cleaning and sanitation products using our unique enzymes that function best in the sanitizing conditions of hot and acidic soulutions. Changes/Problems:At the beginning of this reporting period the project co-director Varun Gejji relocated to Southern California and changed employment. Allison Narlock was already employed at CinderBIo andassumed the majority of his responsibilities for this work. Namely, Allison efficientlyperformed the microbiology, QC, biochemistry,formulationand method development for this work with a high degree of competence and excellence.Parker Brand was hired in May of 2023 and began training on enzyme production to support this project. Parker has proven very competent and has learned the CinderBio fermentation methods and has produced over 600 liters of enzyme-bearing microbial culture which is nearly all processed and added to our inventory to support this work. What opportunities for training and professional development has the project provided?Two technical employees at the bachelor level have been trained in fermentation and downstream processing, biochemstry, and formulation. Allison Narlock has assumed the role of project co-directorhas been trained in project management, microbiology, biochemistry, QC, and customer interface. Parker Brand has been trained in fermentation and downstream processing and the associated engineering and software facets of this work and is beinning his training in biochemistry. Allison and Parker have proven to be aneffective team coordinating enzyme production schedules and experimental planning and progression. How have the results been disseminated to communities of interest?Our publication of work initiated prior to this program reporting removal of biofilms from stainless steel has brought incoming interest from an international firm for cleaning and sanitation of medical devices. In this performance period we have not actively advertized the work in this program as it has beennascent. Given our progress and successful results under this funding we will begin dissemination of our natural cleaning and sanitation capabilities to the public through direct outreach and web-based advertisement. What do you plan to do during the next reporting period to accomplish the goals?We plan to build on our successes and continue with our proposed plan to formulate an all-natural cleaning and sanitation product. We will complete formulation and treatment protocol development to the point of practical use. We plan to feild trial our products on-site with our partner CreamCo Meats and to pursue additional sites for field testing. Depending on resources and timing, we will also begin a marketing effort targeting small and medium sized meat and poultry processors in the Bay Area and surrounding municipalities.
Impacts
What was accomplished under these goals?
Enzyme production and QC, cleaning and sanitation method development andinitial formulation and sanitation experiments have been succesfullyexecuted.Method development for quantitatively measuring both cleaning and sanitation have been established in this reporting period.We have quantatively demonstrated thatenzyme/heat/acid are consistently effective sanitizing treatments for real-world tools used in meat and poultry processing. We have etablished treatment times of 15-30 minutes reduce microbial counts to below detections using methods for measurement that arestandard in the industry irrespective of initial contamination levels. We have established theenzyme re-use limits in cleaning and sanitation of meat processing tools and shown meat soils are broken down and microbial counts are effectively eliminated for a minimum of 10 days of continal use. These data indicate that CinderBio's enzyme/heat/acid natural products are capable of approximately 80 consecutuve days of 30 minute cleaning and sanitation treatments and retain enzyme activities and sanitation efficacy. These data are critical to establish re-use limits and product protocols for our customers.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Nam Y, Barnebey A, Kim HK, Yannone SM, Flint S. Novel hyperthermoacidic archaeal enzymes for removal of thermophilic biofilms from stainless steel. J Appl Microbiol. 2023 Jun 1;134(6):lxad106. doi: 10.1093/jambio/lxad106. PMID: 37218716.
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