Source: NEW MEXICO HIGHLANDS UNIVERSITY submitted to NRP
USING COMBUSTION ELEMENTAL ANALYSIS TO QUANTIFY CARBON AND NITROGEN CONTENT OF RESIDUES FROM FOREST, RANGE, AND AGRICULTURAL MANAGEMENT
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1029333
Grant No.
2022-70410-38457
Cumulative Award Amt.
$34,000.00
Proposal No.
2022-06384
Multistate No.
(N/A)
Project Start Date
Sep 1, 2022
Project End Date
Sep 30, 2023
Grant Year
2022
Program Code
[EGP]- Equipment Grants Program
Recipient Organization
NEW MEXICO HIGHLANDS UNIVERSITY
BOX 9000
LAS VEGAS,NM 87701
Performing Department
(N/A)
Non Technical Summary
We are requesting funding to acquire a combustion elemental analyzer for analysis of soil and plant tissue samples to support forest, range, and agricultural management research. Faculty in the New Mexico Highlands University Department of Forestry are conducting research to compare carbon fluxes associated with different slash treatment methods used for the management of coarse woody debris from forestry operations aimed at reducing fuel loads and fire risk in forest and range ecosystems. Faculty in the New Mexico Reforestation Center in collaboration with New Mexico State University's and the University of New Mexico's Department of Biology are collaboratively researching carbon sequestration potential of reforestation operations in the U.S. Southwest as a function of tree seedling characteristics and planting practices. Finally, the Quivira Coalition is supporting land managers' efforts to improve soil health, which requires them to quantify soil fertility for properties throughout New Mexico. These research projects require chemical analysis of soil and plant samples. A combustion elemental analyzer is the standard equipment for this purpose. It can accurately and rapidly measure the carbon, nitrogen, hydrogen, and sulfur content of biophysical samples. However, at present, there are no facilities in northern New Mexico that can perform these analyses, requiring researchers to ship samples to distant external facilities--a costly expenditure undermining their ability to process the substantive numbers of samples. The purchase of this equipment would help researchers better the productivity and resilience of ecosystems in response to climate change, thereby serving the communities of northern New Mexico.
Animal Health Component
90%
Research Effort Categories
Basic
0%
Applied
90%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1010110107030%
1220430107040%
1230612107030%
Goals / Objectives
Use combustion elemental analyzer in research regarding forest and range managementTrain at least 1 graduate student every year in use of combustion elemental analyzer for Master's thesis research projectsUse combustion elemental analyzer to analyze carbon in soil samples in research comparing the efficacy of herbaceous seed mixes to control erosion in post-wildfire burn scarsUse combustion elemental analyzer to analyze carbon in char and biomass samples in research comparing carbon emissions from various approaches for reducing post-thinning fuel loads, including pile burns, broadcast burns, and biochar productionUse combustion elemental analyzer to analyze carbon in char and biomass samples in Hermit's and Calf Canyon Wildfire burn scarsUse combustion elemental analyzer to analyze carbon in char and biomass samples in research comparing carbon emissions from traditional Maya swidden agroforestry systemsUse combustion elemental analyzer to determine differences among ponderosa pine seed sources in foliar nitrogen and carbon concentrations using three common garden field provenance studiesUse combustion elemental analyzer to determine the impacts of nursery cultural practices on tree seedling nitrogen concentrations (which are reflective of seedling nutritional status) both during nursery production and after planting for reforestation purposes, as well as for directly quantifying the carbon concentration and contents of tree seedlings for purposes of more accurately modeling the carbon sequestration benefits and potential of reforestation operationsUse combustion elemental analyzer in teaching soil science and plant physiology courseworkTrain 10 students enrolled in Soil Science (FORS 4160/5160) at NMHU each year over the lifespan of the equipment to process samples and use the combustion elemental analyzer for analyzing soils for carbon and nitrogenTrain 10 students enrolled in Introduction to Plant Production (FORS 3060) at NMHU each year over the lifespan of the equipment to process samples and use the combustion elemental analyzer for analyzing plant biomass for carbon and nitrogenUse combustion elemental analyzer to help inform land managers' understanding of their land and make management recommendations to increase carbon storageTrain work study student to process and analyze samples collected by land managers in northern New Mexico for carbon and nitrogen
Project Methods
MethodsThe data that are expected to be gathered using this equipment as a part of applied forestry research are total, inorganic, and carbon content; nitrogen; and hydrogen content of plant biomass, char, and soil samples associated with the research projects described below. In all cases, biomass and soil samples will be oven dried at 100F for 24h. Soil samples will be ground and sieved to explude particles with diameter >0.002mm. Biomass and char samples ground and sieved to a particle size between 150-850um. Approximately 5-10mg of samples will be will be placed in a tin foil blank cup, which will then be crimped shut using tweezers and placed in the sampler. The mass of the blank cup, sample, and total packed sample will be recorded in a spreadsheet. When all samples are processed and prepared, the technician or researcher will run the the Costech Analytical Technologies ECS 4010 Nitrogen/Protein Analyzer, using either argon or helium gas as a carrier, depending on availability. The ECS 4010 will output the results, which can be exported to spreadsheet software.Effect of Thinning and Reforestation on Carbon Fluxes in Post-Wildfire LandscapesThis project aims to provide novel science-based solutions for private, tribal, state, and federal forest managers faced with the twin threat of catastrophic fires due to overgrown forests and the inability of post-fire plant communities and ecosystems to naturally regenerate. A part of this project is investigating how microsite environmental conditions influence vegetation community composition during succession after forest thinning operations. Analysis of soil, plant, litter, and woody debris samples to determine their carbon and nitrogen content will be a part of a broader assessment of physical, chemical, and biological characteristics of pre- and post-fire sites. In turn, this would inform and improve land managers' designs for thinning treatments that can reduce forest fuel loads to reduce the risk of catastrophic wildfires while also maintaining ecological productivity and facilitating carbon sequestration. In particular, the combustion analyzer obtained through this grant would allow quantification of carbon fluxes in burned ecosystems by analyzing plant, char, and soil samples collected in areas of different burn severity and historic management.Furthermore, the local availability of a combustion elemental analyzer would allow for much needed local analysis of carbon and nitrogen concentrations and contents in plant and soil samples at the New Mexico Reforestation Center. This capability would support ongoing and future research and operational needs for determining the impacts of nursery cultural practices on tree seedling nitrogen concentrations during nursery production and after planting for reforestation purposes, as well as for directly quantifying the carbon concentration and contents of tree seedlings for purposes of more accurately modeling the carbon sequestration benefits and potential of reforestation operations.Can Biochar Production Reduce Fuel Loads and Improve Soil Health in New Mexican Forests?This project will evaluate whether producing biochar from coarse woody debris stores more carbon for longer (as measured by the ratio of H:C) than other methods of disposing of slash from forest management operations (e.g., chipping, prescribed pile and broadcast burns, lop and scatter, etc.). This research will also compare slash treatment methods on the basis of their ability to maintain soil nutrient levels and reduce fuel loads. The combustion analyzer is essential to quantify the carbon, nitrogen, and hydrogen of char and slash biomass for this research. It will also investigate different biochar application methods to reduce preferential erosion, which could limit the capacity of biochar to effectively sequester carbon in agricultural and forest ecosystems. A combustion analyzer will be necessary to quantify carbon of materials captured in erosion control devices. Both these projects strive to improve the soil health of the agricultural and forest ecosystems of northern New Mexico. In particular, they will improve the capacity of soils to sequester carbon in the long-term, a pressing issue given the impacts of land management on climate change, which is already undermining ecological productivity and increasing wildfire risk in northern New Mexico.Long-term Comparison of Post-Swidden Restoration Approaches in Tropical Maya Lowlands of Chiapas, MexicoThis project is comparing the efficacy of plantation-style and traditional agroforestry restoration to a control (i.e., passive restoration) has been managed by Lacandon Maya farmers in Lacanja Chansayab, Chiapas, Mexico and researchers from New Mexico Highlands University and SUNY ESF. Researchers have annually collected soil samples to evaluate how these restoration treatments have influenced soil fertility in terms of soil organic carbon and nitrogen. Using the elemental combustion analyzer obtained through this grant would allow this long-term research to continue, providing a uniquely long-term perspective on restoration, which is typically only monitored for five years (as opposed to the decade this site has been monitored thus far). It will help identify agroforestry tree species effective at restoring soil fertility while also providing non-timber and timber forest products to marginalized rural communities in degraded post-agricultural landscapes in the tropical lowlands of Chiapas, Mexico.Differences in Photosynthetic Capacity and Foliage Quality among Ponderosa Pine Seed ProvenancesThis project will determine differences among ponderosa pine seed sources in foliar nitrogen and carbon concentrations using three common garden field provenance studies. The garden field provenance study sites are located at the John Harrington Forestry Research Center in Mora, NM; the Arburetum at Flagstaff, AZ; and Brian Head, UT. These sites were established between 2012 and 2019, planting container-grown seedlings from various seed sources along an elevational gradient across Arizona, Utah, and New Mexico and watering them with various amounts of water to simulate different levels of drought stress. The use of elemental analyzer to determine nitrogen and carbon concentrations would provide insights about differences in photosynthetic capacity and foliage quality among these seed sources at each of these sites. These data would be used alongside a growing database of growth and a suite of leaf level physiological and structural traits to understand the mechanisms of establishment for planted ponderosa pine seedlings.Efforts and EvaluationTrained instructors in the NMHU Department of Forestry will lead students enrolled in Soil Science (FORS 4160/5160), and biomass analysis in Plant Production (FORS 3060) in learning about the use of the combustion elemental analyzer. This instruction will often be conducted using curriculum based undergraduate research experiences (CUREs), in which students develop and implement semester-long research projects applying course concepts and familiarizing them with research processes and methods. The impact of this effort will be done through end-of-semester teaching evaluations, which will include a question assessing whether students feel prepared to use this equipment in their future employment.Quivira Coalition staff will lead land manager workshops/field days demonstrating the role of management in developing healthy soils, including advertising the use of this equipment to processs and analyze the soils of land managers in San Miguel and Mora counties. The data from these analyses will be provided to Quivira Coaltion, who can identify management actions to improve soil health. This will be evaluated by the number of land mangers who send in soil samples for analysis, as well as surveys administered by the Quivira Coalition to evaluate outreach program effectiveness.

Progress 09/01/22 to 10/04/23

Outputs
Target Audience:The target audience for this project is threefold. First, we targeted researchers at academic and non-governmental organizations, including those at New Mexico Highlands University and the Quivira Coalition. The acquisition of the combustion analyzer allowed researchers in these organizations to better understand nutrient dynamics in ecosystems and how management could influence them. This was particularly important given the lack of soil and biomass analysis equipment or facilities in New Mexico, providing an opportunity to pursue research that would not have been possible without this project. Next, students at New Mexico Highlands University will benefit from this project. Several departments, including Biology and Forestry, can use this equipment. Undergraduate students will learn how to use combustion analysis for research purposes vis-à-vis coursework, providing them with skills that will be broadly applicable in their careers. Graduate students are now able to use the combustion analyzer for their graduate thesis research. Finally, land managers are able to send soil and plant samples to the Forestry laboratory at New Mexico Highlands University. In turn, the data generated from this equipment can be provided to land managers to better inform their management strategies (e.g., modifying soil conditions through the application of biochar). Changes/Problems:The course--Introduction to Plant Production (FORS 3060)--will not be offered again during the term of this grant. Therefore, we will not be able to train students vis-a-vis this class. What opportunities for training and professional development has the project provided?A lab manager, faculty members (coPI Dixit and PI Falkowski), graduate students were trained in the operation of the use of the Costech Elemental Analyzer (June 19-23, 2023). Furthermore, instructor (PI Falkowski) trained students in the Fall 2023 FORS 4160 class in the use of the combustion analyzer. How have the results been disseminated to communities of interest?We have communicated the availability of this equipment at various community events, and have requested our website developer to update our FORT CREST website to announce this program. While we have not yet received samples for analysis, several land managers have expressed interest in this opportunity, especially in the wake of the Hermit's Peak/Calf Canyon fire. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Of these goals, the following have been completed. Made necessary facilities upgrades (electrical) and installed Costech Elemental Analyzer Trained facutly and lab managers in the use of Costech Elemental Analyzer Trained 2 graduate students in use of combustion elemental analyzer for Master's thesis research projects Used combustion elemental analyzer to analyze carbon in char and biomass samples in Hermit's and Calf Canyon Wildfire burn scars Trained students enrolled in Soil Science (FORS 4160/5160) at NMHU to process samples and use the combustion elemental analyzer for analyzing soils for carbon and nitrogen Processed and analyzed samples collected by land managers in northern New Mexico for carbon and nitrogen The following are still in progress, pending the conclusion of the various research projects using the combustion elemental analyzer purchased with this grant. Use combustion elemental analyzer to analyze carbon in soil samples in research comparing the efficacy of herbaceous seed mixes to control erosion in post-wildfire burn scars Use combustion elemental analyzer to analyze carbon in char and biomass samples in research comparing carbon emissions from various approaches for reducing post-thinning fuel loads, including pile burns, broadcast burns, and biochar production Use combustion elemental analyzer to analyze carbon in char and biomass samples in research comparing carbon emissions from traditional Maya swidden agroforestry systems Use combustion elemental analyzer to determine differences among ponderosa pine seed sources in foliar nitrogen and carbon concentrations using three common garden field provenance studies Use combustion elemental analyzer to determine the impacts of nursery cultural practices on tree seedling nitrogen concentrations (which are reflective of seedling nutritional status) both during nursery production and after planting for reforestation purposes, as well as for directly quantifying the carbon concentration and contents of tree seedlings for purposes of more accurately modeling the carbon sequestration benefits and potential of reforestation operations

Publications


    Progress 09/01/22 to 08/31/23

    Outputs
    Target Audience: Nothing Reported Changes/Problems:Installation took some time given the issues outlined below, and the manufacturers are based in Italy, delaying their travel. However, I am happy to report that the project is back on schedule. From previous progress report: Unfortunately, the electronics of the equipment require a 220V power source, while the power in our lab is 110V. Our facilities department is installing a 220V outlet, but this has slowed the shipment of the equipment. Furthermore, our gasses were not of sufficiently high purity, so we needed to acquire another source of funding to purchase these other gasses and regulators. What opportunities for training and professional development has the project provided?Lab manager (Daryl Williams) and faculty members (Aalap Dixit and Tomasz Falkowski) will be trained in the operation of the Costech Elemental Analyzer (June 19-23, 2023) How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Train a Forestry graduate student in use of combustion elemental analyzer for Master's thesis research projects Use combustion elemental analyzer to analyze carbon in soil samples in research comparing the efficacy of herbaceous seed mixes to control erosion in post-wildfire burn scars Use combustion elemental analyzer to determine differences among ponderosa pine seed sources in foliar nitrogen and carbon concentrations using three common garden field provenance studies. Use combustion elemental analyzer to determine the impacts of nursery cultural practices on tree seedling nitrogen concentrations (which are reflective of seedling nutritional status) both during nursery production and after planting for reforestation purposes, as well as for directly quantifying the carbon concentration and contents of tree seedlings for purposes of more accurately modeling the carbon sequestration benefits and potential of reforestation operations Train students enrolled in Soil Science (FORS 4160/5160) at NMHU in Fall 2023 to process samples and use the combustion elemental analyzer for analyzing soils for carbon and nitrogen

    Impacts
    What was accomplished under these goals? Facilities has wired a new 220v outlet in a shared lab to accomodate equipment Department of Forestry purchased requisite consumables (gases, regulators, etc) Equipment has been ordered and arrived at laboratory Installation and training scheduled for June 19-23 From previous progress report: Purchase of equipment is in process. Obtained quote, PR approved, PO issued.

    Publications