Source: PENNSYLVANIA STATE UNIVERSITY submitted to NRP
SHIMADZU GC-2030 GREENHOUSE GAS CHROMATOGRAPH (GC) FOR A MULTI-USER SOILS RESEARCH LAB
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1029326
Grant No.
2022-70410-38434
Cumulative Award Amt.
$87,200.00
Proposal No.
2022-06375
Multistate No.
(N/A)
Project Start Date
Sep 1, 2022
Project End Date
Apr 7, 2025
Grant Year
2022
Program Code
[EGP]- Equipment Grants Program
Recipient Organization
PENNSYLVANIA STATE UNIVERSITY
408 Old Main
UNIVERSITY PARK,PA 16802-1505
Performing Department
(N/A)
Non Technical Summary
Agricultural activities contribute to global warming by releasing to the atmosphere significant amount of methane (CH4) and nitrous oxide (N2O), two of the most potent greenhouse gasses (GHGs). Agricultural soils are the largest source of N2O emissions in the U.S., contributing 74% of total N2O emissions, while enteric fermentation and emission from manure amount to 35% of total CH4 emissions (EPA, 2022). Considering N2O has a warming potential up to 298 times higher than CO2, understanding the factors contributing to increase N2O emissions and adopting agronomic practices that can minimize greenhouse gas emissions is a critical priority. Indeed, a new 2022-2026 USDA Strategic Plan released in April 2022 identifies its Strategic Goal 1 as to Combat Climate Change to Support America's Working Lands, Natural Resources and Communities. In addition, loss of N as N2O is not only an environmental concern, but also an economic cost for growers both in terms of N fertilizer loss and crop yield loss, because N2O emissions are often accompanied by a much higher loss of N2 that is extremely difficult to measure. Thanks to this funding we will be able to purchaseGas Chromatograph (GC) to measuregreenhouse gasses (CH4, CO2, and N2O). We plan to use this equipment to understand various aspects of global climate change as related to agricultural activities, for example,climate-smartland management(including soybean, corn and barley with and without cover cropping) as well as tillage approaches and nutrient managementto decrease greenhouse gassses emissions.
Animal Health Component
30%
Research Effort Categories
Basic
70%
Applied
30%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1320199107050%
1020110100050%
Goals / Objectives
This NIFA-EGP project will acquirea custom configured Gas Chromatograph (GC) to measuregreenhouse gasses (CH4, CO2, and N2O). We plan to use this equipment to enhance research onvarious aspects of global climate change as related to agricultural activities. The goals of thisinterdisciplinary and broadly collaborative research are (i) to quantify the impact of diversemanagement practices (including soybean, corn and barley with and without cover cropping) ongreenhouse gas emissions; (ii) to develop nitrogen management tools for open field and high-tunnelsoil-based vegetable production system; (iii) to study levels of N2O emissions during and after theanaerobic soil disinfestation (ASD) treatment; (iv) to develop a methodology to continuouslymeasure N2O emissions from soil; (v) to understandmechanisms of GHG emissions in riparianbuffer wells (dissolved gases) and agricultural streams, and (vi) to model N2O, CO2, CH4, and O2in soils and engineered systems (e.g., methane biofilters from livestock facilities) as related toglobal carbon and nitrogen cycles. The GC will be managed by the Soil Research Cluster Lab(SRCL), a shared-use facility in the College of Agricultural Sciences, which provides centrallyaccessible instrumentation for soil analysis and user training and is also open to a broadercommunity ofresearchers.
Project Methods
Gas Chromatograph (GC) enables the measurement of N2O and CH4at ppm concentrations in gas samples. The requested Shimadzu GC system allows for quantitative analysis of major GHGs: methane, carbon dioxide, and nitrous oxide. It employs six flow controllers and two detectors: (1) flame ionization detector (FID) with methanizer for CH4 and CO2, and (2) electron capture detector (ECD) for N2O. The detection limits for CH4, CO2, and N2O are 1 ppm, 10 ppm and 1 ppb, respectively. Autosampler with multiple sample injection modes (gas injection, headspace injection, solid micro-injection) are available and the best way to feed the GC for data reliability and increased productivity. To ensure the researches report quality data, calibrations will be done for every project, every quarter, once per year, or at our discretion. The decision on the calibration frequency will be made based on the nature of the project and instrument performance when doing quality assurance standard checks. The GC will be recalibrated when changing any parameters or consumables. The outcome of GC utilization will be production of chromatogram plots where peak position on horizonal axis is defined by the gas type and area of the peak is used to calculate relative gas amounts. All raw GC data including graphs will be exported in an Excel format (.csv) by the LabSolutions GC management software. Also, custom-designed pdf report with include system and user information, run information (date, settings, parameters) as well as plots and summary results table on the gas concentrations.The data will be shared Ag Data Commons, a public USDA scientific research data catalog and repository (https://data.nal.usda.gov/about-ag-data-commons)?

Progress 09/01/23 to 08/31/24

Outputs
Target Audience:As an instrument at a multi-user facility, the Gas Chromatograph (GC) has been used by students and researchers for training and data collection. Moreover, we have hosted multiple tours for visitors from other organizations and industry, where the importance of greenhouse gas research using GC was highlighted. Changes/Problems:This is not a major problem but worth noting. When the instrument was purchased, we expected higher usage. However, for field studies involving gas sample collection, there are now instruments available that allow measurements of gases in situ, thus making the measurement process more direct and simplified compared to collecting samples in vials and transporting them to the lab for GC measurement. This is specifically advantageous for field studies. Nevertheless, there are still many projects where using a benchtop GC provides the best solution. What opportunities for training and professional development has the project provided?About 10 users has been trained to use intrument indepedently. 12 lab sessions for 6 classes (both undergraduate and graduate) has been developed and conducted. using GC. How have the results been disseminated to communities of interest?The GC collected data for the research projects are being used to produced the manuscripts, yet to be published. The SRCL instrumentation highlighting the GC was presented at the Ag Progress Days,Pennsylvania's largest outdoor agricultural exposition. What do you plan to do during the next reporting period to accomplish the goals?1.We plan to update our lab web page to highlight the capabilities of the instrument and selected research applications. 2.I have started a collaboration with a small business in State College that was recently awarded a National Science Foundation SBIR grant involving analysis of agricultural manures and greenhouse gas measurements. 3.I was granted a Conservation Innovation Grant (CIG) that explores the effect of biochar on greenhouse gas emissions evolved from manure application. This will be a field study where numerous gas samples will be collected and analyzed on our Gas Chromatograph.As part of this project, we will develop a strong extension component to disseminate the results and best management practices of biochar-manure application to Pennsylvania farming communities and other interested stakeholders

Impacts
What was accomplished under these goals? Below are the examples of the selected research projects which utilized the GC of our lab. 1. Camille Bitkus (University of Pittsburg). I used the GC to evaluate the effects of a liposome carrier for nitrogen fertilizer on soil carbon and nitrogen cycling. Gas samples from a 1-week incubation were analyzed for CO2and N2O concentrations. The evolution of CO2concentrations over time revealed that the liposomes served as a carbon source for soil microbes and were promptly consumed. Brief spikes in N2O concentrations indicated potential rapid denitrification. These gas measurements were necessary to capture the microbial consumption of liposomes and to supplement the data collected on changes in soil nitrogen pools over the course of the incubation. Additionally, access to the GC at Penn State was critical, given that there are no GCs for me to use at the University of Pittsburgh or other neighboring institutions. I was able to learn hands-on how to operate a GC, rather than mailing out my samples to another lab. The results from my experiment will be included in a forthcoming publications. 2. Elizabeth Rider (Department Metereology, Penn State). We used the GC to process the field samples collected in Sugar Valley, PA. For my work on CARAT, we are assessing how well the Cycles model is able to capture N2O emissions. To do this, we designed a sampling strategy that we used throughout the summer to collect samples in an agricultural site following manure application. We found that the model, when using site-specific weather information, was able to capture the spike in emissions after application and rainfall events, but failed to reproduce the "baseline" of emissions observed. We plan for more measurements following the spring application! 3. Felipe Montes (Department of Plant Sciences, Penn State). The Shimadzu Nexis GC-2030 Greenhouse Gas Chromatograph Analyzer was used for USDA - Partnerships for Climate Smart Commodities Climate-smart Agriculture that is profitable, Regenerative, Actionable, and Trustworthy (CARAT) project to measure nitrous oxide (N2O), carbon Dioxide (CO2) and Methane (CH4) gas concentration from samples taken from a farmer's field in Loganton, PA. The soil greenhouse gas emissions campaign included 29 sampling sites in a large ryegrass-corn silage rotation field used to produce forage for the dairy farm. Samples were taken 8 separate days over the corn silage growing season. Including calibration standards, close to 600 gas samples were analyzed in the chromatograph. Having access to the Gas Chromatograph Analyzer was essential to achieve the goals of the project.

Publications


    Progress 09/01/22 to 08/31/23

    Outputs
    Target Audience:Shimadzu company has been contacted to place an order for green house gas analyzer.The purchaseorder forShimadzuGreen House Gas Analyzer- number isIN0010790. The order has been placed on 10/31/22.The instrumennt arrived in May 2023. Changes/Problems:See above. The GC autosampler has been damaged by water from the ceiling leak in the lab. What opportunities for training and professional development has the project provided?10 users have been trained on the basics of GC operation from the period of 08/01/2023 and 11/15/2023. We expect more users to be trained and actively use instrument whe autosampler arrives. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?As of now, all laboratory staff are proficient in operating the GC. Training for the autosampler's use is set to be scheduled in February 2023, contingent upon the availability of Shimadzu engineers. Users will be duly informed, and we will start accepting samples and conducting trainings. Currently, samples are outsourced to an external lab.

    Impacts
    What was accomplished under these goals? The purchase order was placed on 10/31/22, and the instrument arrived in May 2023. In May 2023, a ceiling leak from the lab upstairs damaged a crucial component of the instrument--the autosampler. The responsible party has agreed to cover a $20,000 deductible, and the case has progressed through the insurance process. We anticipate receiving funds from the insurance claim in December 2023, allowing us to purchase a new autosampler with a one-month lead time at a cost of $35,000. The absence of the autosampler has adversely affected data acquisition, as manual gas injection is less precise and reliant on operator skill. This slowdown resulted in the delay of data acquision. Shimadzu conducted training on 7/18/2023 on manual injection operation and will re-train users to use autosampler free of charge when it is installed.

    Publications