Source: TEXAS A&M UNIVERSITY submitted to NRP
DEVELOPING SUSTAINABLE ROSE LANDSCAPES VIA ROSE ROSETTE DISEASE EDUCATION AND OUTREACH, SOCIOECONOMIC ASSESSMENTS AND BREEDING RRD RESISTANT ROSES WITH STABLE BLACK SPOT RESISTANCE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
OTHER GRANTS
Reporting Frequency
Annual
Accession No.
1029240
Grant No.
2022-51181-38330
Cumulative Award Amt.
$3,982,555.00
Proposal No.
2022-05300
Multistate No.
(N/A)
Project Start Date
Sep 15, 2022
Project End Date
Sep 14, 2026
Grant Year
2022
Program Code
[SCRI]- Specialty Crop Research Initiative
Recipient Organization
TEXAS A&M UNIVERSITY
750 AGRONOMY RD STE 2701
COLLEGE STATION,TX 77843-0001
Performing Department
(N/A)
Non Technical Summary
Rose Rosette Disease (RRD) is caused by the Rose rosette virus (RRV) and easily transmitted by an eriophyid mite and in infected pre symptomatic plants in commercial channels. It has caused the death of 100s of thousands of plants and annual losses of at least $10 million. If not controlled, it has the potential to devastate the rose industry. The other major disease of rose is rose black spot disease (RBSD). Most rose cultivars are susceptible to these two devastating diseases. Thus, a sustainable landscape needs to have rose cultivars resistant to both diseases. We will characterize the mechanisms of resistance to RRD and develop a genomic platform to develop sustainable roses resistant to both diseases. The mechanism of resistance will be deduced with comparative studies of virus (replication, movement, diversity) and mite (transmission, acquisition, growth, behavior) biology on resistant versus susceptible rose genotypes. A diversity of rose germplasm and segregating populations will be evaluated for resistance using field, greenhouse and laboratory approaches. This phenotypic data will be combined with genotypic data to identify RRD and RBSD resistance QTLs and the associated markers. Haplotype information will be converted to DNA tests to track these resistance genes which will be used to select for genotypes with multiple copies of multiple QTLs that confer resistance to both diseases. The deliverable will be a genomic platform to develop sustainable rose cultivars as well as well characterized germplasm for future breeding. Outreach will involve collaborative research/demonstration trials of the RRD best management practices (BMP), expansion of roserosette.org, and enhanced engagement with all sectors of the rose by leveraging the communication networks of our stakeholders. The Economics Team will develop enterprise budgets for rose productions systems and estimate the socioeconomic impacts of the BMP options to guide the RRD Team in their recommendations.
Animal Health Component
60%
Research Effort Categories
Basic
40%
Applied
60%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2012110108125%
2012110108025%
2162110113010%
2162110110110%
6012110301010%
2162110302020%
Knowledge Area
601 - Economics of Agricultural Production and Farm Management; 216 - Integrated Pest Management Systems; 201 - Plant Genome, Genetics, and Genetic Mechanisms;

Subject Of Investigation
2110 - Ornamental trees and shrubs;

Field Of Science
1101 - Virology; 1130 - Entomology and acarology; 3020 - Education; 1081 - Breeding; 1080 - Genetics; 3010 - Economics;
Goals / Objectives
There is an increasing demand for carefree and sustainable roses that require fewer inputs, are resistant to biotic and abiotic stresses and have high ornamental quality. Sustainable rose cultivars must have resistance to the two most damaging rose diseases in the USA: rose rosette disease (RRD) and worldwide: rose black spot disease (RBSD).The long-term goal of this project is to develop sustainable rose landscapes based on cultivars resistant to RRD and RBSD.Aim 1. Characterize the host plant interaction with RRV and vector Pf mite. This project will characterize RRD resistant roses for their influence on virus biology using an infectious clone reporter system, virus diversity, and mites (reproduction, behavior and transmission efficiency).Aim 2. We will search for additional sources of resistance to RRD and RBSD using field, greenhouse, and laboratory approaches. We will identify and use marker-trait associations to stack genes for RRD/RBSD resistance in rose.Aim 3. Assess the socioeconomic impact of RRD management approaches. Enterprise crop budgets will be created, then used to build a risk-based simulation model to assess the potential social and economic effects of using validated RRD best management practices (BMP) and resistant rose cultivars. This will guide the extension team recommendations based on cost, feasibility, ease of implementation, and consumer confidenceAim 4. Develop comprehensive research demonstration and education programs for RRD management. We will a) perform research demonstrations to validate/optimize the best management practices for RRD in several states, b) will expand the roserosette.org webpage with educational materials for all levels of the industry (consumers, public display gardens, garden centers and producers) and c) improve and expand industry and public garden outreach.
Project Methods
Aim 1. Characterize the host plant relationship with RRV and vector Pf mite The initial research will focus on a rose core set genotypes differing in RRD resistance. This set includes genetically related sets of roses that differ in the RRD QTL LG5 resistance allele dosage and two new distinct sources of resistance.The diploid set consists of 3 genotypes: M4-4, TAMU 7-20 and TAMU-7-20xSE sdlg which are homozygous, heterozygous and null for the resistant RRD LG5 allele. The tetraploid set consists of BExSW null sdlg, Brite Eyes and BExSW duplex sdlg which have the resistant RRD LG5 allele in the nulliplex, simplex and duplex configuration. The two new sources of RRD resistance are the cultivars 'Morden Blush' and 'Morden Fireglow'. This rose core set allows us the ability to compare the effect of resistance sources as well as the dosage effect of one specific RRD resistance QTL on virus and mite biology.The RRV infectious clone will be optimized by testing 4 reporter genes and 4 delivery technologies on both tobacco and rose to select the optimized combination. Once optimized this system will be used to characterize the movement of the virus when inoculated on resistant and susceptible roses to assess the effect that the host plant resistance has on virus movement. The virus diversity will be assessed by systematically sampling virus from a range of roses at multiple sites and analyzing the virus sequence to understand the spatial and ancestral structure of RRV. Any isolate that responds differentially among locations or predicted to react differentially to the virus or mite will be substituted into the infectious clone and tested for differences in RRV pathogenicity or symptom development.To assess if RRD field resistance affects mite biology, colonies of mites will be established on both RRV and RRV-infected plants. Comparative studies of RRD resistant and susceptible cultivars were assessed for RRV transmission/acquisition efficiency on whole plants with the mites restricted in Munger cells and for antibiosis, mite size and behavior using a detached leaf/shoot approach to assess whether the level of RRD resistance influences mite biology. A parallel field study will follow the mite populations on resistant/susceptible rose genotypes to better understand how mite population dynamics are affected by the level of RRD resistance in the rose genotype.Aim 2. Establish a breeding platform to enable the development of adapted and commercially acceptable RRD/RBSD-resistant roses.We will search for additional sources of resistance utilizing field, greenhouse and laboratory protocols to ensure the development of stable resistance to the mite, virus and fungus. We will search for new genetic factors controlling resistance and the associated SNP marker(s) by genotyping (GBS or SNP array) and phenotyping mapping populations and a diversity panel (cultivar trial). The analysis will involve programs such as FlexQTL, polymapR, polyqtlR, MapPoly, QTLpoly, GWAS poly, PolyOrigin, diaQtl, and Viewpoly. SNPs that are associated with RRD/RBSD resistance will be converted into a DNA-test format to enable marker assisted selection for the presence of RRD and RBSD resistance loci and to identify and develop rose germplasm where multiple genes for resistance have been stacked. This germplasm will facilitate the development of durably resistant rose cultivars needed for future sustainable gardens and landscapes.We will use parents that are segregating for resistance for both diseases. We will continue to develop populations of interrelated families that can be analyzed as a group using FlexQTL (Bink et al., 2014) on the diploid level, and PolyOrigin (Zheng et al., 2020) and diaQTL (Amadeu et al., 2020) on the tetraploid level. As stated before, the strategy is to develop germplasm that have SNP-tagged factors for subsequent breeding and pyramiding.Aim 3. Assess the socioeconomic impact of RRD management approaches Consensus production enterprise budgets for field and container rose production systems will be developed through grower interviews. With these budgets, the potential economic and social impacts of using validated BMPs and resistant cultivars will be assessed using risk-based simulation models. This analysis will guide the extension team in developing BMP recommendations based on cost, feasibility, implementation ease and consumer confidenceAim 4. Develop comprehensive research demonstration and education programs for RRD managementWe will launch multiple research demonstrations, including locations with industry and public garden partners and validate/optimize the best management practices for RRD. The web resources and social media networks of the horticultural industry and public gardens will be leveraged to disseminate disease incidence and RRD management information. This includes expanding and enhancing the roserosette.org webpages with educational materials for all levels of the industry (consumers, public display gardens, garden centers, and producers). Industry and public garden outreach will be improved with collaborative monitoring activities. Outreach efforts will be coordinated with annual meetings among researchers, the outreach team, and industry.

Progress 09/15/23 to 09/14/24

Outputs
Target Audience:The target audience for this project is quite broad. It starts with the Rose Industry, which includes commercial propagators and nurseries, landscape management companies, private rose breeders, independent nursery operations, agrochemical companies, consultants and the major trade organization AmericanHort. It also includes federal regulatory agencies (IR4, National Clean Plant Network), plant diagnostic laboratories, researchers with state, federal and private organizations, and the gardening public and their organizations (American Rose Society and the major rose testing programs (ARTS, AGRS, AOE and Earth-Kind®). Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The PIs, graduate students and postdoctoral researchers participated in professional meetings (Plant and Animal Genome, American Society of Horticultural Sciences) and the Tools for Polyploids Training Workshop (San Diego, Jan. 2024). In addition, the Sustainable Rose SCRI Project in-person meeting was held on April 22, 2024. The meeting was attended by project PIs, collaborators and the project's Advisory Board. How have the results been disseminated to communities of interest?During the first year of the project, we communicated progress to our scientific colleagues through articles and presentations and the rose community/industry through trade journal articles and rose community/industry presentations. The extension team has given x presentations and have established variety trial research demonstrations in 9 locations and 4 states. The Sustainable Rose project website (RoseRosette.org) received 55,642 pageviews from 26,219 users (- ~ 4549 views per month and ~2,430 visitors per month). The X account (https://twitter.com/SustainableRRD) has 23 followers and we are building a following with industry partnerships and relevant researchers What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Obj 1.1 Characterize RRV-host interaction RRD-susceptible and RRD-resistant/immune roses. Obj 1.1.1 Optimize the RRV infectious clone-reporter system A method based on young-rooted cuttings was used to study the vascular movement of RRV. In plants with a single shoot, RRV was detected in other leaves at 3 weeks, 100% of the top leaves, and 90% of the roots at 9 weeks. In inoculated plants with >2 shoots, RRV spread to other shoots and roots. Thus, RRV can move between shoots, likely through viral MPs and the host active transport machinery. Obj 1.1.2 Characterize the RRV-host interaction using the RRV-reporter system Young-rooted cuttings were used to determine whether RRV segments 5, 6, and 7 are required for virus systemic infection. Inoculating plants with a single shoot with an infectious RRV clone where RNA6 or RNA7 were deleted did not affect the virus's ability to spread to the top leaf. Obj 1.1.3 Virus diversity assessment Three viral segments of RRV Texas strains were synthesized to be tested for their impact on virulence. Obj 1.2 Characterize the mite-host interaction Obj 1.2.1 Characterize the host suitability for Pf RRV transmission Once we rebuild the collapsed mite populations, we will start graft inoculation trials and mite inoculation trials. We will use small cooling units for the mite colonies in the greenhouse; duplicate colonies will be maintained in separate growth chambers. Cooling units will also be used to maintain graft-inoculated plants within tent cages with 25 µm screen windows. We have developed 3-D printed grafting jigs to improve graft inoculations. Obj 1.2.2 Characterize the host suitability for Pf reproduction and feeding. We have developed 3-D printed cages to enclose rose flowers for studies of comparative mite reproduction and longevity on the core set of roses to gain information on resistance mechanisms. Scanning electron microscopy evaluation of relative trichome density on sepals will start soon. Obj 1.2.3 Field studies of Pf mite populations. Mite field population trails were established in TN (including the rose core set). Roses were augmented with symptomatic RRD infected roses. Mite population counts will be conducted, RRD severity will be recorded and samples for qPCR will be collected in September 2024. Obj 2.1. Identify and characterize new sources of RRD and RBSD resistance Obj 2.1.1 Assemble germplasm We have a germplasm collection of ~1,375 unique genotypes composed of mapping populations and families involving germplasm with resistance to RRD (Morden Blush and Morden Fireglow), and partial (Miracle on the Hudson) and dominant (Ramblin Red, G168-2, Frau Dagmar Hartopp) black spot resistance. This collection also includes ~100 rose cultivars and selections. A diversity panel of another ~400 varieties representing the diversity of the cultivated germplasm was assembled. Obj 2.1.2 Perform cultivar and seedling trials in three locations Approximately 1,375 plants were established in OK, TN, and TX. The roses in OK and TN have been augmented with RRV. RRD severity scores and tissue for qPCR evaluations are being collected. The roses in TX were evaluated for black spot, Cercospora, and defoliation. Obj 2.1.3 Evaluation of race specific RBSD resistance At UWRF, rose black spot resistance/susceptibility phenotyping was expanded to 50 rose genotypes with six key races known to differentiate Rdr genes (races 3, 8, 9, 11, 12 and 13). An additional 200 rose genotypes (known to be resistant to RRD and relatives of RRD-resistant roses) are being phenotyped with these races. Additionally, roses developed at Ag Canada and relatives of roses with known Rdr genes are being phenotyped. Obj 2.1.4 Optimize greenhouse assay for RRV resistance A system using young-rooted cuttings was established. Plants are inoculated with an infectious RRV clone or sap from an infected rose plant, and inoculum is delivered to the L3 leaf of a plant. Subsequently, leaves closest to the shoot apical meristem is collected at 9 weeks post-inoculation and RT-PCR is used to detect RRV in the collected sample. Obj 2.2 Discovery and deployment of RRD and RBSD resistance QTL Obj 2.2.1 Genotypic analysis of plant materials Germplasm in fields in OK, TN, and TX has been genotyped with ~68K SNPs using an Axiom array. The material consists of mapping populations and families representing ~606 unique individuals and ~129 varieties. In TN, a metagenome-wide sequencing was used to quantify and profile the rose leaf-associated metagenome of RRV inoculated roses. The approach revealed P. fructiphilus as the sole eriophyid mite species. No viruses were identified, likely due to the sample preparation method, which will be repeated. The method revealed other pathogens including black spot. Obj 2.2.2 Development of KASPTM and PACETM assays At UWRF, 14 SNPs converted to KASP/PACE markers were validated at UWRF on 63 roses used in the original validation of Rdr3. The 3 most promising markers were subsequently tested on ~ 500 additional roses. 12 SNPs associated with the Rdr6 locus have been selected from BSAseq (bulk segregant analysis using sequencing data) at UMN. They are being converted to PACE markers for future testing at UWRF. At UMN, linkage mapping of the Rdr5 and Rdr6 resistance loci has been completed. Also, a BSAseq approach was used to identify SNPs more tightly linked to Rdr6. Candidate gene mining is nearly complete for both loci. Obj 2.2.3. Stack multiple genes for resistance We identified major QTLs for partial black spot (LG3 and LG5), RRD (LG5), and Cercospora (LG1 and LG4) resistance. Individuals carrying multiple disease resistance QTL and with optimal genome estimated breeding value for disease resistance were used in crosses. Obj 3.1 Construct enterprise crop budgets Data was gathered from online resources with information on commercial rose production, articles on rose production methods and costs, and industry manuals and reports for detailed production practices. The data revealed significant differences in costs associated with different production methods. For example, field production is labor-intensive but has lower capital costs compared to container production. Though more expensive to transport, liner plants are easier to plant and establish than bare root plants. Budded plants had higher upfront costs due to grafting, but led to higher quality and yield over time. Obj 3.2. Estimate socioeconomic impact of improved RRD BMP Once the cost of production budgets is refined, the socioeconomic impact will be measured by the end of year 3. Obj 4.1. Conduct research demonstrations to validate/optimize the best management practices for RRD. Variety research demonstration trials (19 rose cultivars) were established in Texas (2 sites), MOBOT (1), Ohio (2), Tennessee (1) and Georgia (4). These roses have been rated for RRD and black spot severity. Also, a new mobile reporting platform developed in collaboration with the RRD Extension team has been successfully tested to collect data. Obj 4.2. Enhance roserosette.org The Sustainable Rose project website (RoseRosette.org) received 98,841 views from 27,000 users in 30,054 visits. 14 reporters involved with the project submitted 2,408 plant ratings to EDDMapS. Obj 4.3. Improve Industry and Public Garden Outreach The team gave presentations at industry and rose community meetings, including the 2024 Fall Gardener's Festival at UT Gardens, Crossville, Tenarky District of the Am. Rose Society, Nashville, the UT garden, Knoxville, Memphis Botanical Garden, Central, Eastern, and Western extension regions in TN, Master Gardener training in Tarrant, Denton, and Kaufman counties in TX, the OH Turfgrass Foundation Conference, the Mid-OH Growers Association Annual Meeting, the Southeast Green Industry Update, the UGA ANR Extension Agents, the Bibb County Master Gardeners, the Advanced Master Gardener Training, Athens, and the GGIA Southeast Green Conference, Duluth, GA

Publications

  • Type: Journal Articles Status: Published Year Published: 2024 Citation: Rawandoozi, Z.J., A. Barocco, M.Y. Rawandoozi, P.E. Klein, D.H. Byrne, and O. Riera-Lizarazu. 2024. Genetic dissection of stem and leaf rachis prickles in diploid rose using a pedigree-based QTL analysis. Front. Plant Sci. 15: 1356750. doi: https://doi.org/10.3389/fpls.2024.1356750
  • Type: Other Status: Awaiting Publication Year Published: 2024 Citation: Ranabhat, N.B. and Windham M. 2024. Rose Rosette Disease: A quick overview an extension factsheet,University of Tennessee Extension.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Byrne, D. 2023. Introduction to the meeting. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Baum, D. and J. LaForest. 2023. Website and upcoming features. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Ong, K., J. Williams-Woodard, F. Peduto-Hand, and M. Windham. 2023. General overview of disease cycle and current best management practices. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Palma, M. 2023. Overview on previous work. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Ribera, L. 2023. Workshop on enterprise budget construction. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Verchot, J. Hammond, and R. Jordan. 2023. Virus diversity and host fitness. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Atalla, O., and J. Verchot. 2023. RRV host range. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Ochoa-Corona, F., K. Ong, and R. Jordan. 2023. Update on diagnostics for RRV. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Byrne, D., and O. Riera-Lizarazu. 2023. Field resistance to rose rosette disease. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Ali, A., and F. Ochoa-Corona. 2023. Oklahoma: Field trials for RRD resistance. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Ranabhat, N., and M. Windham. Tennessee: Field trials for RRD resistance. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Ong, K., J. Williams-Woodard, F. Peduto-Hand, M. Windham, J. LaForest. 2023. Extension demonstration trials: variety and miticide trials. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Ochoa, R. 2023. Overview on eriophyid mite vectors of RRD. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Joseph, S. 2023. Temporal incidence of eriophyid mites. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Hammond, J., and R. Jordan.2023. Mite Biology and RRD resistance. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Byrne, D., J. Lau, and O. Riera-Lizarazu. 2023. Black spot resistance in rose: field evaluations. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Zlesak, D., D. Arias Lopez, and S. Hokanson. 2023. Black spot resistance in rose: detached leaf evaluations. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Arias Lopez, D., D. Zlesak, and S. Hokanson. 2023. Black spot resistance in rose: detached leaf evaluations. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Lau, J., Z. Rawandoozi, D. Byrne, and O. Riera-Lizarazu. 2023. Black spot resistance: field resistance. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Hochhaus, T., J. Lau, C.H. Taniguti, E. Young, D. Byrne, O. Riera-Lizarazu. 2023. Meta-analysis of rose rosette disease resistance quantitative loci and search for candidate genes. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Riera-Lizarazu, O., D. Byrne, and P. Klein. 2023. Genomics approach for sustainable rose development. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Riera-Lizarazu, O., P. Klein, D. Byrne. 2023. GWAS experiment to find additional genes for resistance. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Olukolu, B. 2023. Metagenomics. Sustainable Roses Project Meeting. Crossville, TN, Nov. 16-17, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Hochhaus, T., Lau, J., Taniguti, C.H., Young, E., Byrne, D.H., Riera-Lizarazu, O. Candidate gene search for rose rosette disease resistance in the Rosa wichurana Basyes Thornless genome assembly. Tools for Polyploids Workshop (San Diego, CA). Poster. January 10, 2024.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Hochhaus, T., Lau, J., Taniguti, C.H., Young, E., Byrne, D.H., Riera-Lizarazu, O. Integrated Quantitative Trait Loci Mapping for Rose Rosette Disease Resistance and a Search for candidate Genes in Rose (Rosa spp.) . American Society for Horticultural Sciences Conference(Orlando, FL). August 3, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Lopez Arias, D. C. 2024. Rose Black Spot Resistance: From Loci Detection to KASP Marker Development. Poster presentation at II CFANS (College of Food, Agricultural and Natural Resource Sciences) research Symposium of the University of Minnesota, Saint Paul, MN. Award winning poster.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Moore, Emily M., D. C. Lopez Arias, D. C. Zlesak, M. D. Clark, D. Yong, J. Sproul, and S. C. Hokanson. 2024. Finding Resistance: Evaluating Two Approaches for Identifying Rose Black Spot Disease Resistance (Rdr) Loci in Polyploid Garden Rose. Plant and Animal Genome Conference. San Diego, California.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Hand, F.P. Diseases of landscape ornamentals and their management. 30-min oral presentation at the Ohio Turfgrass Foundation Conference & Show, Columbus, OH. December 17, 2023. 85 participants.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Hand, F.P. Diseases of floriculture crops. 45-min oral presentation at the OSU Extension Pesticide Applicator Training Educators In-service, Columbus, OH. December 13, 2023. 30 participants.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Hand, F.P. Identifying and controlling flower diseases, 60-min oral presentation at the Mid-Ohio Growers Association Annual Meeting, Millersburg, OH. January 11, 2024. 250 participants.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Goel, S., Cook, C., Bledsole, O., Ueckert, J. and Ong, K. 2024. Monitoring and Mitigation of Rose Rosette Virus: Comparative study from 2018 to present. Texas A&M BESC Summer REEU poster symposium. August 1, 2024.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Hochhaus, T., Lau, J., Taniguti, C.H., Young, E., Byrne, D.H., Riera-Lizarazu, O. Candidate gene search for rose rosette disease resistance in the Rosa wichurana Basyes Thornless genome assembly. Plant and Animal Genomics Conference 31 (San Diego, CA). Poster. January 15, 2024.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Ranabhat,N. B. Updates on Sustainable Rose Project Research and Extension, 1 hr oral presentation at Tenarky District of the American Rose Society, Winter workshop, Nashville, TN, Feb 17, 2024 (40 participants)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Ranabhat, N.B. Disease updates on ornamentals: especial focuses on rose diseases UT Rose Garden tour talking about identification and management of rose diseases including RRD at Green Industry Field Day, UT garden, Knoxville, TN, June 26-27, 2024 (62 participants, talk on the rose garden)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Ranabhat, N.B. Memphis Botanical Garden tour with plant pathologist talking about Ornamentals diseases including RRD at Horticulture Insect and Disease Workship, Memphis Botanical Garden, Memphis, TN, May 30, 2024 (31 participants, talk on the rose garden)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Ranabhat, N.B. Rose rosette diseases: early detection, spread, and host-virus-vector interaction, EPP 509, June 4, 2024- (10 students)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Ranabhat, N.B. Plant Health Diagnosis: Approaches and Strategies Central region extension agents In-service, Rutherford County Extension Office, Murfreesboro, TN, July 11, 2024 (27 participants)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Ranabhat, N.B. Plant Health Diagnosis Eastern region extension agents In-service, Eastern Region Extension Office, Knoxville, TN, May 24, 2024 (11 participants)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Ranabhat, N.B. Plant Health Diagnosis: Approaches and Strategies Western region extension agents In-service, Jackson, TN, August 23, 2024 (17 participants)
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Verchot, J. 2024. Using Rose rosette infectious clone to track systemic virus infection in hybrid rose plants. 2024 American Phytopathological Society, Caribbean Division Meeting, M�rida, Yucat�n, M�xico. April 15  17, 2024
  • Type: Websites Status: Published Year Published: 2024 Citation: Sustainable Roses. https://roserosette.org/
  • Type: Theses/Dissertations Status: Published Year Published: 2024 Citation: Gill, H. 2024. The game of colors in roses: QTL, Transcriptomic, and pigment analysis of the color transition phenotypes in autotetraploid rose populations. Ph.D. Dissertation, Texas A&M University, College Station, TX, USA
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Williams-Woodward, J.L. Rose and Boxwood disease update. 40-minute oral presentation for Southeast Green Industry Update (Zoom), Athens, GA. March 8, 2024.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Williams-Woodward, J.L. Ornamental Disease Update. 120-minute oral presentation to UGA ANR Extension Agents, Athens, GA. March 20, 2024.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Williams-Woodward, J.L. Ornamental Disease Update. 90-minute oral presentation and tour of rose plots for Bibb County Master Gardeners, Athens, GA. April 12, 2024.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Williams-Woodward, J.L. Landscape Disease Workshop. 60-min oral presentation at the GGIA Southeast Green Conference, Duluth, GA. June 4, 2024.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Williams-Woodward, J.L. Diseases of landscape ornamentals 60-min oral presentation at the Advanced Master Gardener Training, Athens, GA. August 30, 2024.


Progress 09/15/22 to 09/14/23

Outputs
Target Audience:The target audience for this project is quite broad. It starts with the Rose Industry which includes commercial propagators and nurseries, landscape management companies, private rose breeders, independent nursery operations, agrochemical companies, consultants and the major trade organization AmericanHort. It also includes federal regulatory agencies (IR4, National Clean Plant Network), plant diagnostic laboratories, researchers with state, federal and private organizations, and the gardening public and their organizations (American Rose Society and the major rose testing programs (ARTS, AGRS, AOE and Earth-Kind®). Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The PIs, graduate students and post-doctoral researchers participated in professional meetings (Plant and Animal Genome, 11th Rosaceae Genomics Conference, American Society of Horticultural Sciences) and in the Tools for Polyploids Training Workshop (San Diego, Jan., 2023). How have the results been disseminated to communities of interest?During the first year of the project, we have communicated progress to our scientific colleagues (10 journal articles, 17 presentations) and the rose community/industry (press release,trade journal article, a podcast, 6 rose community/industry presentations). The extension team has given 10 presentations and have established variety trial research demonstrations in 9 locations and 4 states. The RoseRosette.org was redesigned, updated and rebranded as the Sustainable Rose project website with a new logo. During the past year, the site has received 69,346 pageviews from 27,655 users (~2,300 visit per month). Created X account (https://twitter.com/SustainableRRD) and are building the followship by following sponsors, collaborators, and members' labs and posting publications and information relevant to the project. A short summary of the project was developed and sent out to several collaborators for the inclusion in their communication to their clientele. Developing a mailing list to keep our clientele informed of our progress. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Aim 1. Characterize the host plant interaction with RRV and vector Pf mite. Objective 1.1 Characterize the RRV-host interaction RRD-susceptible and RRD-resistant/immune roses. Obj 1.1.1 Optimize the RRV infectious clone-reporter system Developed RRV inoculation protocol with infectious clone which showed a higher infection rate on young plants (80%, 3 weeks after inoculation) than did the sap inoculated plants. The virus moves up and down a branch within 3 weeks of inoculation. Work is ongoing to assess how/when it moves to adjacent branches. Obj. 1.1.2 Characterize the RRV-host interaction using the RRV-reporter system We have optimized the RNA extraction protocol to yield high quality RNA which allows the RNA-seq for transcription profiling of individual leaves post inoculation in mature and young roses. Obj. 1.1.3 Virus diversity assessment Genetic analysis of 95 RRV isolates indicated 85% of these had genome segment reassortment. The non-recombinant types grouped into two lineages. This suggest an early series of adaptations from the wild host Rosa multiflora to cultivated roses, followed by slower adaptation and genetic drift within the many cultivated rose genotypes. Several viral proteins appear to be evolving independently, suggesting that diversity may be important for adaptation to new host genotypes and resistance genes. The diversity among RRV isolates appears greater than previously recognized. Objective 1.2 Characterize the mite-host interaction Obj. 1.2.1 Characterize the host suitability for Pf RRV transmission Obtained RRV-infected plants with rose bud mites (Phyllocoptes fructiphilus) to ensure maintenance of infected RRV source plants in the Beltsville South Farm and established mites on a rose in a clear plastic tent cage with panels of 25 μm polyester mesh to allow air exchange without allowing mites to escape. Clip-on leaf cages were modified with 25 μm polyester mesh were used to grow Pf on rose plants in the laboratory. Developed a RRV graft inoculation technique. Obj. 1.2.2 Characterize the host suitability for Pf reproduction and feeding. See above. Obj. 1.2.3 Field studies of Pf mite populations. Trial to be planted in the spring of 2024 Aim 2. Objective 2.1. Identify and characterize new sources of RRD and RBSD resistance Obj. 2.1.1 Assemble germplasm Assembled germplasm for QTL validation for RRD, and black spot resistance with the focus on unique sources of resistance to RRD (Canadian cv), and partial(Miracle on the Hudson) and dominant (Ramblin Red, Baby Love, Frau Dagmar Hartopp) black spot resistance. The variety trial includes about 100 rose cultivars (retesting cv, Canadian and Buck cv, new cv) and ~65 TAMU selections (Rosa rugosa, palustris, setigera, carolina hybrids, promising selections). Currently assembling a diversity panel (~400 rose genotypes) representing the diversity of the cultivated repeat or recurrent blooming rose germplasm to assess for resistance to disease and various horticultural traits. Obj. 2.1.2 Perform cultivar and seedling trials in three locations Established genetic populations and variety trial in 2022 and 2023.1,800 to 2,400 plants were established in each location (OK, TN, TX). The roses in OK and TN have been augmented with RRV by attaching infested shoots to the test plants. The roses in TX were evaluated for black spot, cercospora, defoliation and horticultural traits. Obj. 2.1.3 Evaluation of race specific RBSD resistance Fifty-five additional seedlings of 'Morden Blush' by 'GeorgeVancouver' were phenotyped with race 3 and 8 to determine if Rdr3 confers partial resistance to race 3. Rdr5 and Rdr6 were mapped. Bulked segregant analysis performed to discover SNPs tightly linked to Rdr6. Obj. 2.1.4 Optimize greenhouse assay for RRV resistance Objective 2.2 Discovery and deployment of RRD and RBSD resistance QTL Obj. 2.2.1 Genotypic analysis of plant materials Analyses of the field ratings of a diverse set of roses (~150) revealed that the high level of resistance for each disease is from a distinct set of germplasm. Thus, the need to actively stack multiple resistances from distinct germplasm into the cultivated rose germplasm. Analyses in both diploid and tetraploid rose populations identified major QTLs for partial black spot (LG3), rose rosette disease (LG5) and cercospora leaf spot (LG1 and LG4) resistance. Beyond these major QTLs, multiple minor QTLs were also identified. A set of parents were selected from these populations using both their QTL genotype and their estimated breeding value for disease resistance for the first set of crosses in the stacking process. Other traits (defoliation, flower size, prickle density, plant architecture, flower intensity) are being mapped and analyzed for QTL to facilitate their assessment as a parental. The datasets for various studies were uploaded to the Genome Database for Rosaceae. Metagenome-assisted analyses for identifying QTL and multipartite interactions that underlie the rose rosette disease (RRD). Initial work is validating an inexpensive and high-throughput library preparation and sequencing protocol, OmeSeq-qRRS, for quantitative and strain-level metagenome sequencing of the rose caulosphere (stem) and phylloplane (leaves). This wholistic approach will deliver titer values for the mites, RRV, and other organisms that might play direct or indirect (through interaction with mites and RRV) roles in the variability of RRD severity and symptoms and aid in identifying QTLs that confer resistance/tolerance against the mites and RRV. Obj. 2.2.2 Development of KASPTM and PACETM assays KASP marker development for Rdr3 is ongoing with validation work with ~150 roses including ancestors and descendents containing Rdr3. Amplicons in the Rdr3 region are being sequenced to discover tightly linked SNP markers to develop additional KASP markers. Obj. 2.2.3. Stack multiple genes for resistance First set of crosses made to stack disease resistance genes. Aim 3. Assess the socioeconomic impact of RRD management approaches. Objective 3.1 Construct enterprise crop budgets We have developed a template for enterprise budget and will organize producer panels to develop the budgets. Objective 3.2. Estimate socioeconomic impact of improved RRD BMP No progress yet as need to complete enterprise crop budgets first. Aim 4. Develop comprehensive research demonstration and education programs for RRD management. Objective 4.1. Conduct research demonstrations to validate/optimize the best management practices for RRD. Have established or establishing variety research demonstration trials (19 rose cultivars) in Texas (Baylor University), Missouri (Missouri Botanical Garden), Ohio (Wooster and Springfield), Tennessee (Crossville) and Georgia (Watkinsville, Cartersville, Chatworth and Perry). These will be evaluated for RRD and black spot incidence. Plans are being made to establish research demonstration trials to assess the efficacy of chemical control. Objective 4.2. Enhance roserosette.org RoseRosette.org was redesigned, updated and rebranded as the Sustainable Rose project website with a new logo. During the past year, the site has received 69,346 pageviews from 27,655 users (~2,300 visit per month). There were 904 reports of Rose Rosette Disease sightings in the landscape. These reports were in 371 counties in 45 states. 2 were verified as new county reports for RRD. Beta versions of the reporting system for collecting disease ratings at the variety trials have been developed. Created Twitter account (https://twitter.com/SustainableRRD). Objective 4.3. Improve Industry and Public Garden Outreach Developing a mailing list, developed a summary of the RRD research for distribution, recorded a podcast on the Rose Chat site and have given presentations at multiple horticulture industry and rose community meetings.

Publications

  • Type: Journal Articles Status: Published Year Published: 2022 Citation: Claros, N.A., M. Shires, D. Mollov, J. Hammond, R. Jordan, F. Ochoa-Corona, J. Olson, K. Ong, and L. Rodriguez Salamanca. 2022. Rose rosette disease: A diagnostic guide. Plant Health Progress. https://doi.org/10.1094/PHP-05-22-0047-DG
  • Type: Journal Articles Status: Published Year Published: 2023 Citation: Farber, C., Shires, M., Ueckert, J., Ong, K., & Kurouski, D. (2023). Detection and differentiation of herbicide stresses in roses by Raman spectroscopy. Frontiers in Plant Science, 14, 1121012. https://doi.org/10.3389/fpls.2023.1121012
  • Type: Journal Articles Status: Published Year Published: 2023 Citation: Verchot J, Herath V, Jordan R, Hammond J. 2023. Genetic Diversity among Rose Rosette Virus Isolates: A Roadmap towards Studies of Gene Function and Pathogenicity. Pathogens. 2023; 12(5):707. https://doi.org/10.3390/pathogens12050707
  • Type: Journal Articles Status: Published Year Published: 2022 Citation: Rawandoozi, Z.J., E.L Young, M. Yan, S. Noyan, Q. Fu, T. Hochhaus, M.Y. Rawandoozi, P.E. Klein, D.H. Byrne, O. Riera-Lizarazu, 2022. QTL mapping and characterization of black spot disease resistance using two multi-parental diploid rose populations, Horticulture Research, Volume 9, 2022, uhac183. https://doi.org/10.1093/hr/uhac183.
  • Type: Journal Articles Status: Published Year Published: 2023 Citation: Rawandoozi Z.J., E.L. Young, S. Kang, M. Yan, S. Noyan, Q. Fu, T. Hochhaus, M.Y. Rawandoozi, P.E. Klein, D.H Byrne, O. Riera-Lizarazu. 2023. Pedigree-based analysis in multi-parental diploid rose populations reveals QTLs for cercospora leaf spot disease resistance. Front Plant Sci. 6;13:1082461. https://doi.org/10.3389/fpls.2022.1082461.
  • Type: Journal Articles Status: Published Year Published: 2023 Citation: Lau, J., Gill, H., Taniguti, C. H., Young, E. L., Klein, P. E., Byrne, D. H., et al. 2023. QTL discovery for resistance to black spot and cercospora leaf spot, and defoliation in two interconnected F1 bi-parental tetraploid garden rose populations. Frontiers in Plant Science 14. https://www.frontiersin.org/articles/10.3389/fpls.2023.1209445
  • Type: Journal Articles Status: Published Year Published: 2022 Citation: Lau, Jeekin, Ellen L. Young, Sara Collins, Mark T. Windham, Patricia E. Klein, David H. Byrne, and Oscar Riera-Lizarazu. 2022. Rose Rosette Disease Resistance Loci Detected in Two Interconnected Tetraploid Garden Rose Populations. Frontiers in Plant Science 13: 916231.
  • Type: Journal Articles Status: Published Year Published: 2023 Citation: Windham, M.T., T. Evans, S. Collins, J.A. Lake, J. Lau, O. Riera-Lizarazu and D.H. Byrne. 2023. Field resistance to rose rosette disease as determined by multi-year evaluations in Tennessee and Delaware. Pathogens 2023 112(3):439.
  • Type: Journal Articles Status: Published Year Published: 2023 Citation: Hochhaus, T., J. Lau, C. Taniguti, E. Young, D.H. Byrne and O. Riera-Lizarazu. 2023. Meta-Analysis of Rose Rosette Disease-Resistant Quantitative Trait Loci and a Search for Candidate Genes. Pathogens 2023, 12, 575. https://doi.org/10.3390/patho-gens12040575
  • Type: Journal Articles Status: Published Year Published: 2023 Citation: Atallah, O.O., S.M. Yassin, N. Shirley, and J. Verchot. 2022. Exploring the Host Range of Rose rosette Virus among herbaceous annual plants. Pathogens 2022, 11, 1514. https://doi.org/10.3390/pathogens11121514
  • Type: Other Status: Published Year Published: 2023 Citation: Byrne, D.H., O. Riera-Lizarazu, and M. Windham. 2023. Developing sustainable rose cultivars of the future. American Rose 47(1):60-71.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Rawandoozi Z.J., Young E.L., Kang S., Yan M., Noyan S., Fu Q., Hochhaus T., Rawandoozi M.Y., Klein P.E., Byrne D.H., Riera-Lizarazu O. 2023. Pedigree-based analysis in multi-parental diploid rose populations reveals QTLs for fungal diseases. Poster. The 11th Rosaceae Genomics Conference, Plant & Food Research, Nelson, New Zealand. March 15, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Lau, J., H. Gill, C.H. Taniguti, E. Young, P.E. Klein, D.H. Byrne, O. Riera-Lizarazu. 2023. Black Spot and Cercospora Leaf Spot Resistance in Auto-Tetraploid Garden Roses. Oral presentation. 11th Rosaceae Genomics Conference. Nelson, New Zealand. March 15, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Hochhaus, T., Lau, J., Taniguti, C.H., Young, E., Byrne, D.H., Riera-Lizarazu, O. 2023. Meta-analysis and identification of candidate genes for rose rosette disease resistance quantitative loci. Poster. 11th Rosaceae Genomics conference, Nelson, New Zealand. Poster. March 15, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Byrne, D.H., O. Riera-Lizarazu and J. Endelman. 2023. Tools for genomics-assisted breeding in polyploids SCRI project. Oral presentation. 11th Rosaceae Genomics Conference, Nelson, New Zealand. March 13-16, 2023
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Riera-Lizarazu, O. and D.H. Byrne. 2023. Towards genomics-aided rose breeding in Texas, USA. Oral presentation. 11th Rosaceae Genomics Conference, Nelson, New Zealand. March 13-16, 2023
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Gill H., Lau J., Zhen S., Byrne D., and Riera-Lizarazu O. Genetic Determinants and Pigment Analysis of Autotetraploid Roses Exhibiting the Flower Color Transition Phenotype. Poster. 11th Rosaceae Genomics Conference, Nelson, New Zealand. March 13-16, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Lau, J., Young E. L., Gill G., Rawandoozi Z., Taniguti C.H., Hochhaus T. M., Klein P. E., Byrne. D. H., Riera-Lizarazu O. 2023. Breeding for disease resistance in autotetraploid roses. Oral. XXX Plant & Animal Genome Conference. San Diego (CA), United States. January, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Tessa Hochhaus. Meta-analysis of rose rosette disease resistance quantitative trait loci and a search for candidate genes. Tools for Polyploids Workshop Poster Session 1/13/23. https://shorturl.at/acpI6
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Haramrit Gill. Identification of Quantitative Trait Loci Controlling Shoot Length, Internode Length, and Number of Internodes in Tetraploid Roses. Tools for Polyploids Workshop Poster Session 1/12/23. https://rb.gy/fq4ch
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Hochhaus, T., Lau, J., Taniguti, C.H., Young, E., Byrne, D.H., Riera-Lizarazu, O. 2023. Integrated Quantitative Trait Loci Mapping for Rose Rosette Disease Resistance and a Search for Candidate Genes in Rose (Rosa spp.). American Society for Horticultural Sciences Conference (Orlando, FL). Oral presentation. August 3.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Lopez Arias, D.C. 2023. Rdr5, a Novel Rose Black Spot Disease Resistance Locus. XXX Plant & Animal Genome Conference. San Diego (CA), United States. January, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Taniguti, C. H., Taniguti, L. M.; Amadeu, R. R.; Lau, J.; De Siqueira Gesteira, G.; Oliveira, T. D. P.; Ferreira, G. C.; Pereira, G. D. S.; Byrne, D.; Mollinari, M.; Riera-Lizarazu, O.; Garcia, A. A. F. 2023. Selecting best bioinformatic pipeline for evaluating diploid and auto-tetraploid garden roses genotyping-by-sequence data. Oral. XXX Plant & Animal Genome Conference. San Diego (CA), United States. January, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Hochhaus, T., Lau, J., Taniguti, C.H., Young, E., Byrne, D.H., Riera-Lizarazu, O. 2023. Meta-analysis of rose rosette disease resistance quantitative loci and search for candidate genes. Poster. XXX Plant & Animal Genome Conference. San Diego (CA), United States. January, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Gill H., Lau J., Whiteley L., Byrne D., and Riera-Lizarazu O. Identification of Quantitative Trait Loci Controlling Shoot Length, Internode Length, and Number of Internodes in Tetraploid Roses. Poster. XXX Plant & Animal Genome Conference. San Diego (CA), United States. January, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Moore, E. 2023. Identification of a novel rose black spot disease resistance locus (Rdr6) in a tetraploid Baby LoveTM derived population. American Society for Horticultural Sciences Conference (Orlando, FL). Oral presentation. August 3
  • Type: Other Status: Published Year Published: 2023 Citation: Byrne, D.H. 2023. Research journey of sustainable roses. Rose Chat Podcast. Rose Chat Podcast | a podcast by VanCleave Media Group (podbean.com). Feb, 26, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Byrne , D.H. and O. Riera-Lizarazu. 2023. Developing Sustainable Rose Landscapes. Lone Star Horticulture Forum, Texas Nursery and Landscape Association, College Station, TX. Jan 9, 2023
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Byrne, D.H. and O. Riera-Lizarazu. 2023. Developing Sustainable Rose Landscapes. National Convention of the American Rose Society, Shreveport, LA, May 6, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Byrne, D.H. and O. Riera-Lizarazu. 2023. The Beauty and the Beasts. The Journey towards Sustainable Roses for the Garden. American Rose Society New York District Convention and Rose Show. Binghamton, NY. Sept 9, 2023
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Ong, K. 2023. Scouting for plant Health. Education program at the Texas Nursery & Landscape Association Expo 2023, San Antonio, TX. August 9, 2023
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Ong, K. 2023. Status of Rose Rosette Disease in the US. Southern Plant Board meeting, Gulf Shores, March 23 ,2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Hand, F.P. 2023. Diseases of nursery crops: the not so good, the really bad, and the ugly. 60-min oral presentation at the Tri-State Green Industry Conference, Cincinnati, OH. February 2, 2023
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Hand, F.P. 2023. Diseases of nursery crops: the not so good, the really bad, and the ugly. 60-min oral presentation at the Nursery Growers of Lake Co. Ohio Association Meeting, Madison, OH. March 7, 2023
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Hand, F.P. 2023. Scouting for ornamental plant diseases in our gardens. 90-min oral presentation at the Tri-County Master Gardener Volunteers Diagnostic Update, Akron, OH. March 14, 2023
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Williams-Woodward, J.L. 2023. Ornamental Disease Update. 120-minute oral presentation to UGA ANR Extension Agents, Athens, GA. March 16, 2023
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Williams-Woodward, J.L. 2023. Landscape Disease Workshop. 60-min oral presentation at the GGIA Southeast Green Conference, Duluth, GA. June 14, 2023
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Williams-Woodward, J.L. 2023. Diseases of ornamental shrubs. 90-minute oral presentation to Green Industry Update, Valdosta, GA. June 22, 2023
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Williams-Woodward, J.L. 2023. Leaf spots, viruses, and root rots. 60-minute oral presentation to Green Industry Update, Cairo, GA. July 20, 2023
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Williams-Woodward, J.L. 2023. Diseases of landscape ornamentals. 60-min oral presentation at the Statewide Advanced Master Gardener Training, Athens, GA. August 11, 2023
  • Type: Websites Status: Published Year Published: 2023 Citation: Byrne, D., Ong, K., Baum, D., LaForest, J., Willams-Woodward, J., Hand, F., & Windham, M. (2023, June 1). Developing sustainable roses through education and breeding. Sustainable Roses. Retrieved August 31, 2023, from https://roserosette.org