Progress 09/15/23 to 09/14/24
Outputs
Target Audience:Diagnosticians at land grant universities (LGU), state departments of agriculture (SDA), plant pathologists, entomologists, nematologists, weed scientists, extension educators, growers and producers, crop consultants, Master gardeners, the public Changes/Problems:Increased costs for personnel (salary and benefits) and supplies have decreased the power of NPDN funds. Labs are increasingly questioning the value of the time commitment that NPDN membership entails. It has been increasingly difficult to recruit diagnosticians to take on leadership roles on NPDN committees. Diagnosticians that commit to committee leadership roles are receiving additional funds. What opportunities for training and professional development has the project provided?In April 2023, diagnosticians at Iowa State University's Plant Diagnostic Laboratory hosted the region for a multi-day regional meeting. The meeting began at Corteva, where we toured their diagnostic facilities and had discussions about diagnostics, impacts the NDR has on private industry and possible ways to collaborate. Day 2 of the meeting included presentations from faculty at ISU and covered a variety of topics including delusory parasitosis, grape vine decline, a new pathogen of corn, plant pathology communications, the Crop Protection Network, and NDPN Core Accreditation. The meeting concluded with state reports where members shared highlights of their year. Jan Byrne led the Accreditation Committee's efforts to conduct a hybrid meeting to help members begin the process of accreditation. The meeting included presentations and work sessions. The one and a half day workshop was held in Beltsville, Maryland and offered in a hybrid format; it was attended by 57 diagnosticians from across the country. Feedback from attendees prompted the committee to hold additional online sessions in shorter time periods, several drop-in sessions were hosted by NCPDN members. How have the results been disseminated to communities of interest?Many LGU diagnosticians have extension or outreach roles and use in person workshops, training, lab websites, University extension communication services (fact sheets, images, etc), and social media to disseminate current information to our clients and communities. For example, University of Wisconsin and MSU's diagnostic labs maintain Twitter accounts, Wisconsin's Department of Agriculture, Trade and Consumer Protection emails a weekly newsletter, and Purdue publishes a Purdue Landscape Report. Diagnosticians in the region produced 11refereed publications and 102non-refereed publications. Over 100 extension and 19 scientific conference presentations were given. What do you plan to do during the next reporting period to accomplish the goals?The NCPDN will: Continue to meet monthly to discuss issues related to diagnostics and appropriate methods. Provide travel funds for a minimum of one diagnostician per sub award to attend NCPDN regional meetings and NPDN national diagnostic meeting. Provide funds to attend professional society meetings that are relevant to diagnostics. Encourage regional representation on the NPDN Committees. Have regional representation and provide guidance to the Lab Accreditation Committee. Develop and offer accreditation work sessions to diagnosticians preparing for core accreditation. Represent the region on national NPDN committees Leadership will meet as a group 1-2 times per month to discuss on work on national priorities and initiatives.
Impacts
What was accomplished under these goals?
1. Detection Iowa's diagnosticians noted several first reports on the specific host, which included several bacterial pathogens, one fungal, and an aphid. Illinois has several first detections on hemp including Fusarium flower blight and two insects. Indiana had several first detections of pathogens identified to the species level including Phytophthora capsica on Sassafras, Pythium myriotylum on sweet basil, and Diplodia cupressi on Rocky Mtn. Juniper. Michigan's diagnosticians had first reports of beech leaf disease, caused by a nematode and Ralstonia solanacearum on Osteospermum in California. A first report of Ralstonia was also made in Minnesota, from ginger. Missouri published a first report of charcoal rot on hemp (https://apsjournals.apsnet.org/doi/10.1094/PDIS-02-23-0385-PDN). Pepper thrips were a first detection in Wisconsin. Refer to Appendix A for more information. 2. Diagnosis In 2023, NCPDN diagnosticians processed over 17,000 samples using a variety of diagnostic methods. Almost half of these samples were evaluated for pathogens. Nematodes (4,835) and arthropods (1,959) made up large proportions of the rest of the samples. Labs in Illinois and Michigan also offer resistance testing for both herbicides and fungicides. Laboratory methods used at each lab vary slightly, but all labs use molecular techniques (conventional or real-time PCR) including sequencing to identify pests. Collectively NCPDN labs uploaded 1,664, 724, and 100 diagnoses using conventional, real-time PCR, and sequencing methods respectively. Purdue University has found sequencing is a convenient way to resolve diagnoses that otherwise could not be made beyond a more general level. Diagnosticians attribute a confidence level to each uploaded diagnosis; approximately 10,000 samples had a diagnosis with a confirmed level of confidence. Also important are those samples with a "not detected" confidence level, compromising 5,926 diagnoses. For many clients knowing that a particular pest or pathogen is not present is just as valuable as having one confirmed. Testing offered through NPDN labs is cost-effective for growers looking to screen plant material for specific pathogens, often viral pathogens. 3. Reporting In the time period of January 1, 2023, through December 31, 2023diagnosticians in the region uploaded 17,429diagnoses from 14,078samples to the NPDN national data repository (NDR). There were 35 first detections communicated to regulatory officials. NCPDN diagnosticians have good relationships with their regulatory partners. Indiana does not have a regulatory lab and Purdue fulfills that role. The lab is Wisconsin is a regulatory lab, this relationship has helped build better connections with the NPDN regulatory committee and the regulatory community at large.
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2023
Citation:
Luis, J., Mehl, H., Plewa, D., and Kleczewski, N. 2023. Is Microdochium maydis associated with necrotic lesions in the tar spot disease complex? A culture-based survey of maize in Mexico and the Midwest United States. Phytopathology. 113: 1890-1897. https://apsjournals.apsnet.org/doi/10.1094/PHYTO-04-23-0109-R
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
John Hammond, Dimitre Mollov, Ronald Ochoa, Ramon L. Jordan, Todd Rounsaville, Emily Johnson, Samuel Grinstead, Karen K. Rane, Tom C. Creswell . 2023. Natural host range, putative vector, and diversity of Lindera severe mosaic-associated virus, a recently identified emaravirus.. Poster, APS Plant Health. https://www.ars.usda.gov/research/publications/publication/?seqNo115=360928
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Guan, W, J Bonkowski, T. Creswell, D Egel.2023. Strawberry Cultivar Susceptibility to Neopestalotiopsis leaf spot in Indiana.. Plant Health Progress,. (Editors pick Oct 30, 2023) https://doi.org/10.1094/PHP-05-22-0049-RS
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Critchfield, R., J. King, J. Bonkowski, D. Telenko, T. Creswell, L Zhang. 2023 Characterization of Virulence Phenotypes of Heterodera glycines during 2020 in Indiana.. J. Nematology, 2023 Feb; 55(1), doi: 10.2478/jofnem-2023-0039
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
First Report of Colletotrichum fioriniae Infecting Hop (Humulus lupulus) in Michigan
Ross J. Hatlen, Rebecca M. Swift, Laura A. Miles, Jan M. Byrne, and Timothy D. Miles Plant Disease 2023 107:10, 3280
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
First report of anthracnose on sugar beet (Beta vulgaris) caused by Colletotrichum incanum in Michigan, USA. L. E. Hanson, E. M. Schlachter, D. H. Minier, J. Byrne, J. F. Willbur
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Salgado JS, Alvarez-Quinto R, Bauman M, Arenz BE, Lockhart B, and Mollov D (2023) First Report of Tobacco Rattle Virus Infecting Weigela florida in the United States Plant Disease 107:9, 2894 https://doi.org/10.1094/PDIS-02-23-0351-PDN
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Goodnight, Karen Morgan, Peng Tian, and Kaitlyn M. Bissonnette. "First Report of Charcoal Rot Caused by Macrophomina phaseolina on Hemp (Cannabis sativa) in Missouri." Plant Disease 107.9 (2023): 2856.
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Progress 09/15/22 to 09/14/23
Outputs
Target Audience:Diagnosticians at land grant universities (LGU), state departments of agriculture (SDA), plant pathologists, entomologists, nematologists, weed scientists, extension educators, growers and producers, crop consultants, Master gardeners, the public. Changes/Problems:Increased costs for personnel (salary and benefits) and supplies have decreased the power of NPDN funds. Labs are increasingly questioning the value of the time commitment that NPDN membership entails. It has been increasingly difficult to recruit diagnosticians to take on leadership roles on NPDN committees. Diagnosticians that commit to committee leadership roles are receiving additional funds. The lab accreditation manager will be hired at theNEPDN regional center(University of Maine) instead of the NCPDN regional center (Michigan State University). What opportunities for training and professional development has the project provided?The NCPDN continues to emphasize training for diagnosticians to enhance diagnostic ability. Diagnosticians within the region continue to add assays to their testing capability, molecular detection capacity for Agrobacterium, Rhodococcus, Bretziella fagacearum, phytoplasmas have increased in several labs. Fourteen members attended workshops that were coordinated by NPDN and offered by USDA staff (Laurel, MD). Topics included bioinformatics, isothermal amplification, Phytophthora, phytoplasmas, and potato wart. Seven members attended other professional conferences. The North Central region is represented by Brett Arenz (MN) and Chelsea Harbach (IA) on the NPDN Professional Development Committee. This committee has developed an onboarding checklist for new diagnosticians, which continues to be used and valued by new NPDN members. How have the results been disseminated to communities of interest?Many LGU diagnosticians have extension or outreach roles and use in person workshops, training, lab websites, University extension communication services (fact sheets, images, etc), and social media to disseminate current information to our clients and communities. For example, University of Wisconsin and MSU's diagnostic labs maintain Twitter accounts, Wisconsin's Department of Agriculture, Trade and Consumer Protection emails a weekly newsletter, and Purdue publishes a Purdue Landscape Report. Diagnosticians in the region produced 12 refereed publications and 61 non-refereed publications. Over 100 extension and 17 scientific conference presentations were given. What do you plan to do during the next reporting period to accomplish the goals?NCPDN is working with an assessment specialist who guided some changes to the annual capacity and accomplishment survey that all subcontractors complete annually. Additionally, data is being analyzed to identify changes over time in capacity, professional development, and lab scope. Guidelines to capture examples of the impact of NPDN in diagnostic cases stories will be developed by the assessment specialist. The region will continue to meet monthly to discuss issues related to diagnostics and appropriate methods. NDPN members will continue to represent the region on national NPDN committees. NCPDN leadership will meet as a group 1-2 times per month to discuss on work on national priorities and initiatives.
Impacts
What was accomplished under these goals?
Goal #1 - Detection Two labs (Iowa State and Ohio State) had staffing changes; three new diagnosticians were hired to fill vacancies at these labs. The new diagnosticians have brought additional expertise and approaches to diagnostics and client services. The NCPDN region includes eight states, each with a land grand university diagnostic lab that is a member institution. Additionally, six of the NC states have state department of agriculture diagnosticians and staff that are engaged members or partners of NPDN. This legacy of networking and interaction has improved communication and collaborative work during regulatory events such as the recent detection of Ralstonia solanacearum in imported geraniums. A diagnostic lab in each of the states within the NCPDN uploads data to the NPDN NDR. Data is uploaded using a set of universal host and diagnosis codes. Daily emails are sent to NPDN members which include a list of the codes that were used for the first time in that state. This serves as a notification of possible first detections for diagnosticians and engaged regulatory members. NCPDN funds were used to purchase reagents and maintain qPCR equipment needed to provide testing for regulatory pathogens (ex. P. ramorum, tomato brown rugose fruit virus). The equipment is also used to provide cost-effective testing for viruses of hops, grapes, and other niche crops that is otherwise cost prohibitive. Growers use this service to screen new plant material prior to establishment in their fields. This supports good IPM practices, economically viable agriculture industries, and food security in our region. Goal #2- Diagnostic Support The region holds annual meetings for our members; the 2022 meeting was held at UC Davis as part of the national NPDN meeting, members attended either in person or virtually. Regional monthly conference calls are held to promote networking, sharing of diagnostic challenges, discussions of new protocols, and provide onboarding support to new diagnosticians. A part time lab accreditation manager position was established and posted. A hybrid lab accreditation workshop was developed and planned; over 50 NPDN members registered to attend. The workshop will review the NPDN core accreditation standard and allow attendees to work on their own lab quality manual, the first step in the lab accreditation process. Goal #3- Reporting In the time period of January 1, 2022, through December 31, 2022 diagnosticians in the region uploaded 18,101 diagnoses from 13,747 samples to the NPDN national data repository (NDR). There were 307 first uploads from the NCPDN, some but not all, represent new pest detections. Some of the first detections included Peronospora trifoliorum on lupine, Ceratocystis fimbriata on sweet potato, Corynespora cassiicola in soybean, Phytophthora kelmanii on Canaan fir, Litylenchus crenatea on beech, and multiple instances of Ceratobasidium theobromae.
Publications
- Type:
Other
Status:
Published
Year Published:
2022
Citation:
Beckerman, J., Creswell, T. (2022) Symptoms and Signs for Plant Problem Diagnosis - An Illustrated Glossary; Factsheet https://mdc.itap.purdue.edu/item.asp?itemID=24577
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Bonkowski J, Crow WT, Habteweld A. (2022) First Report of Belonolaimus longicaudatus Infecting Soybean in Indiana. J Nematol. Nov 12;54(1). https://doi.org/10.2478/jofnem-2022-0034
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Bonkowski, J, Ruhl, G., Creswell, T. (2022) First report of Exserohilum pedicellatum causing root rot of corn in Indiana Plant Disease. https://doi.org/10.1094/PDIS-06-21-1245-PDN
- Type:
Websites
Status:
Published
Year Published:
2022
Citation:
Crain, B., Cregg, B., Byrne, J., (2022) Leaf scorch or oak wilt: Whats plaguing my tree? MSU Extension News. Sept 26, 2022.
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Crizon Cortes S., Hanson L., Miles L. A., Wilbur J., and Naegele R. (2022) Diagnostic guide for Alternaria leaf spot on sugar beet, red beet, and chard. Plant Health Prog. 23:497-506.
- Type:
Websites
Status:
Other
Year Published:
2022
Citation:
Hammerschmidt, R., A. Tenney, and E. Hill. (2022) Nematode diagnostics, a changing of the guard at MSU Plant & Pest Diagnostics. Michigan State University Extension News. October 7, 2022. Available online at: https://www.canr.msu.edu/news/nematode-diagnostics-a-changing-of-the-guard-at-msu-plant-pest-diagnostics
- Type:
Websites
Status:
Other
Year Published:
2022
Citation:
Hill, E. and E. Patterson. (2022) 2022 Status of herbicide-resistance weeds in Michigan. Michigan State University Extension News. September 10, 2022. Available online at: https://www.canr.msu.edu/news/2022-status-of-herbicide-resistant-weeds-in-michigan
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Hanson, L.E., Schlachter, E.M., Minier, D.H., Byrne, J. & Willbur, J.F. (2023) First report of anthracnose on sugar beet (Beta vulgaris) caused by Colletotrichum incanum in Michigan, USA. New Disease Reports, 47, e12152. https://doi.org/10.1002/ndr2.12152
- Type:
Journal Articles
Status:
Accepted
Year Published:
2022
Citation:
Hatlen R., Swift R. M., Miles L. A., Byrne J., and Miles T. D. (2022) First report of Colletotrichum fioriniae infecting Humulus lupulus in Michigan. Plant Dis. Accepted with revisions.
- Type:
Journal Articles
Status:
Awaiting Publication
Year Published:
2022
Citation:
Heger, L., Giraud, D., Miles, L., Byrne, J., and Miles, T.D. (2022) First report of Neofusicoccum rubis causing stem blight and dieback of blueberry (Vaccinium corymbosum). Plant Disease (in press). https://doi.org/10.1094/PDIS-07-22-1519-PDN
- Type:
Journal Articles
Status:
Accepted
Year Published:
2022
Citation:
Kaishan, P., Creswell, T., Bonkowski, J. Aime C. (2022) First report of smoketree rust, caused by Pileolaria cotini-coggygriae, in the Midwest USA. Plant Health Progress, 2022, https://doi.org/10.1094/PHP-08-22-0080-BR
- Type:
Journal Articles
Status:
Accepted
Year Published:
2022
Citation:
Maclot, F.J., Mandujano, M., Nakasato, K., Byrne, J., Paudel, S., Guyer, D., Malmstrom, C. (2022) First report of tobacco ringspot virus infecting pawpaw orchard (Asimina triloba (L.) Dunal) in North America. Plant Disease (in press). https://doi.org/10.1094/PDIS-11-22-2639-PDN
- Type:
Journal Articles
Status:
Accepted
Year Published:
2022
Citation:
Weisberg, A., Ruitta, E., Kilday,K., Bonkowski, J., Creswell, T., Daughtrey,M., Rane,K., Grunwald, N., Chang, J., Putnam, M. (2022) Whole genome sequencing-based tracing of a 2022 introduction and outbreak of Xanthomonas hortorum pv. pelargonii. Phytopathology, 2022, https://doi.org/10.1094/PHYTO-09-22-0321-R
- Type:
Journal Articles
Status:
Awaiting Publication
Year Published:
2022
Citation:
Willbur, J. Schlachter, E.M., Satoh-Cruz, M., Rush, S., Burek, C., Byrne, J. (2022) First report of Geotrichum candidum causing rubbery rot of potato (Solanum tuberosum) in Michigan. Plant Disease
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