Progress 09/15/23 to 09/14/24
Outputs
Target Audience:Our primary target audience are stakeholders in the production and distribution of sweet corn. Our stakeholders include members of sweet corn breeding programs in seed companies, processors who decide on hybrids for contracts with growers, and sweet corn growers. Changes/Problems:Challenging environmental conditions delayed several projects. Unlike previous winters, there was substantial insect damage in the winter nursery in Puerto Rico 2023/24. As such, crosses of the best 11 HMF/SHGD sweet corn genotypes with Wf9 and A427 are being repeated during the summer of 2024. Conversely, low pest pressure in early 2024 in south Florida prevented field collection of corn silk fly larvae for adult production in the lab. This was compounded by poor laboratory corn silk fly colony performance, together delaying laboratory research on corn silk fly adult behavioral modification. Extreme drought reduced leaf disease infections in Wisconsin. The field experiment will need to be repeated, possibly with irrigation to increase leaf disease pressure. Hiring the Clemson postdoctoral researcher to participate in this project was much slower than anticipated, with multiple candidates rejecting offers over the course of the first year and a half of the project. The advertised salary was increased with a supplement from non-project funds, and a qualified candidate accepted the offer to join the project in April 2024 with a start date of July 1 2024. What opportunities for training and professional development has the project provided? ?Wilson, A.R. (2023, August). Breeding specialty corn. Vegetable Breeding Institute at Cornell University, Ithaca, NY. Fakude, M., Frei, U. K., Foster, T. L., and Lübberstedt, T. (2023, November, 09 - 12). Identification of genomic regions associated with the causal QTL of SHGD trait in Ames panel by GWAS [Poster]. 2023 ASA, CSSA, SSSA International Annual Meeting, St. Louis, MO, USA. Graciano, R. (2023, December, 04-05). Exploring the application of phenomic selection in sweet corn breeding [Oral presentation]. International Sweet Corn Development Association 2023 Annual Meeting, Orlando, FL, USA. Graciano, R. (2024, February, 28-29). Exporing the application of phenomic selection in corn breeding [Oral presentation]. Maize Genetics Meeting - Corn Breeding and Research Meeting, Raleigh, NC, USA. Johnson, B., and Beuzelin, J. (2023, October, 18). Development of a reliable marking method for corn silk flies [Oral Presentation]. Gulf Coast Post-Doc and Student Association 2023 Inter-REC Poster and Flash Talk Competition, Wimauma, FL, USA Johnson, B., et al.(2024, July, 01-03). Examining the movement of corn silk flies (Diptera: Ulidiidae) into sweet corn fields [Oral Presentation]. Annual Meeting of the Florida Entomological Society, Quincy, FL, USA. Johnson, B., Beuzelin, J., Allan, S., Diepenbrock, L., and Hahn, P. (2024, June, 9-11). Marking corn silk flies with fluorescent dyes for improved management [Oral Presentation]. Annual meeting of the Florida State Horticultural Society, Orlando, FL, USA. McCluskey, C.A., & Tracy, W.F. (2023, September 20.) On-farm genetic diversity of maize: Data and defining the blanks. [Oral presentation.] German Conference for Geography, Goethe University, Frankfurt, Germany. Peixoto, M. and Resende Jr., M.F.R. (2023, December, 04-05). Leveraging genomic models through imputation of low-density marker data in sweet corn [Oral presentation]. International Sweet Corn Development Association 2023 Annual Meeting, Orlando, FL, USA. Pereira Lima, L., Beuzelin, J., Seal, D., Allan, S. (2024, March, 18). Attractiveness of hydrolyzed protein baits to corn silk flies (Diptera: Ulidiidae) [Oral Presentation]. Annual Meeting of the Southeastern Branch of the Entomological Society of America, Augusta, GA, USA. Thompson, A.M. (2023, November 6-7). CGM, GS, & Phenomics to enable prediction of GxE.M system. [Oral presentation]. GxExM Modeling Workshop, Gainesville, Florida USA. Viana, M. and Resende Jr., M.F.R. (2023, December, 04-05). K-mer-based GWAS in sweet corn [Oral presentation]. International Sweet Corn Development Association 2023 Annual Meeting, Orlando, FL, USA. Aboobucker, S., Zhou, L., Frei, U.K., and Lübberstedt, T. (2024, February 29 - March 03). Parallel spindle genes restore haploid male fertility - removing a bottleneck in doubled haploid technology [Poster]. 2024 Maize Genetics Meeting, Raleigh, NC, USA. Fakude, M., et al. (2024, April, 14-17). Genomic Mapping of Haploid Male Fertility, Haploid Female Fertility, and Haploid Frailty in BS39 and BS39-SHGD maize lines [Poster]. Advances in Genome Biology and Technology (AGBT) 2024 Agricultural Meeting, Phoenix, AZ, USA. ?Graciano, R. (2024, March, 01-03). Exploring the application of phenomic selection in sweet corn breeding [Poster]. Maize Genetics Meeting, Raleigh, NC, USA. Graciano, R.P., Peixoto, M., Suzuki, N., Gustin, J., Armstrong, P.R., and Resende, M.F.R. (2024, July, 22-26). Exploring the application of single kernel phenomic selection in corn breeding. 7th International Conference of Quantitative Genetics. Vienna, Austria. Johnson, B., Beuzelin, J., Allan, S., Diepenbrock, L., and Hahn, P. (2024, June, 19). Targeted corn silk fly control: Marking evaluation, movement analysis, behavioral manipulation, and biological control potential [Poster]. Farm Foundation Cultivators Round Table Meeting, Broomfield, CO, USA. Liu, H., Gorman, Z., Maurer, H., Sorg, A., Basset, GJ., and Block, A.K. (2024, April 01). Identification of a maize coumarate-CoA ligase involved in the production of anti-herbivore compound chlorogenic acid [Poster]. University of Florida Spring 2024 Undergraduate Research Symposium, Gainesville, FL, USA. McCluskey, C.A., and Tracy, W.F. (2023 September 21.) On-farm genetic diversity of maize: Data and defining the blanks. [Poster.] German Conference for Geography, Goethe University, Frankfurt, Germany. Viana, M., Colantonio, V., Peixoto, M., Leach, K., and Resende Jr., M.F.R. (2024, March, 01 - 03). Comparing K-mer-based and SNP-based GWAS approaches for trait improvement in sweet corn [Poster]. Maize Genetics Meeting, Raleigh, NC, USA. Yactayo-Chang, J.P., et al.(2024, Feb 29 -March 03). Maize Terpene Synthase 1 impacts insect pest behavior via the production of monoterpene volatiles [Poster]. 66th Annual Maize Genetics Meeting, Raleigh, NC, USA. Beuzelin, J., and Johnson, B. (2024, April, 05). Insect ecology and management in crops of the Everglades Agricultural Area [Seminar].UF/IFAS Everglades Research and Education Center - Faculty Programmatic Seminar Series, Belle Glade, FL, USA. Lübberstedt, T. (2023, October, 16). Updates on Doubled Haploid Technology in Maize [Seminar]. RAGT Research Center, Druelle, France. Lübberstedt, T. (2023, October, 17). Efficient Breeding of Resilient Crops Using Doubled Haploid Technology [Seminar]. INRAE, AgroParisTech, Le Moulon, France. Lübberstedt, T. (2024, April, 20). Haploids: from obscure Phenomenon to Doubled Haploids to vigorous flowering Plants [Seminar]. ETH Zürich, Switzerland. Thompson, A.M. (2023, October 3). Maize Genome to Phenomes: Insights, Nitrogen Response, Disease Resistance. [Seminar]. Plant Sciences Seminar, University of Arizona, Tucson, Arizona USA. Thompson, A.M. (2023, November 17). Exploring maize resilience through genetics, phenomics, and canopy architecture. [Seminar] University of Nebraska Agronomy and Horticultural Seminar, Lincoln, Nebraska USA. Beuzelin, J. (2024, April, 12). Corn silk fly update [Oral Presentation]. Sunshine Sweet Corn Farmers of Florida Meeting, Belle Glade, FL, USA. Beuzelin, J., and Johnson, B. (2023, December, 07). Sweet corn insect ecology and management [Oral Presentation]. UF/IFAS Sweet Corn Management Workshop, Belle Glade, FL, USA. McCluskey, C.A. (2024, May, 9). Data blanks by design: Maize standing diversity, intellectual property, and restrictions on public research. [Invited guest lecture]. The Evergreen State College, Olympia, Washington, U.S. Mitchell, P.D. (2024, June, 5). Sweet CAP Research Update. Midwest Food Products Association's Board of Directors Meeting, Wisconsin Dells, WI. Paul Mitchell organized and led a "Processing vegetable consumption trends and opportunities" Focus Group (2024, February, 28) with midwestern staff from Lakeside Foods and from Harris Moran Seed Company in Sun Prairie, Wisconsin (~20 attendees). Thompson, A.M. (2024, February 12 - 15). NAPPN 2023 Early Career Award talk. North American Plant Phenotyping Network, West Lafayette, Indiana USA. Thompson, A.M. (2024, February 28). [Invited speaker]. Corn Breeding Research Meeting, Raleigh, North Carolina, USA. Addie Thompson hosted a Corn Field Day for Michigan corn growers and graduate students. Addie Thompson hosted the teacher workshop and tour for Education Project's "Feeding and Fueling the World: a Workshop for Nebraska Teacher" (2024, June 13 - 14) in Lincoln, NE, USA. Ursula Frei organized and led the Doubled Haploid Workshop (2024, August, 01-02) at Iowa State University, Ames, IA, USA. Marco Peixoto organized and led the Alphasim Workshop (2023, November, 13-15) at the University of Florida, Gainesville, FL, USA. How have the results been disseminated to communities of interest?In the current two-year reporting period (09/01/2022 - 08/31/24) results were primarily disseminated through peer-review publications (25 published articles, 6 in review, 6 in preparation), oral and poster presentations (34 in total) at regional and international academic conferences, seminars (12), and various outreach events and workshops (13). The results were also presented formally and informally to growers' association in Florida, Washington, and Wisconsin, including: -the American Seed Trade Association's Vegetable and Flower Technical Subcommittee Field Crop Seed Convention Phytosanitary Committee -the Midwest Food Products Association's Board of Directors -the International Sweet Corn Development Association -the Sunshine Sweet Corn Farmers of Florida Finally, the germplasm developed in the scope of this project was showcased to sweet corn seed companies and other stakeholders in field trials in Florida, New York, and Wisconsin. What do you plan to do during the next reporting period to accomplish the goals?Objective 1. Breeding technologies 1.1: Build the sweet corn pan-genome and develop a DNA imputation pipeline. Evaluate the imputation accuracy using the Practical Haplotype Graph and compare them with standard DNA imputation methods. 1.2: Develop and employ methods for GS in public sweet corn public breeding programs. Genotype new DH lines being created and select superior ones based on a combination of genomic selection + phenomic selection. We will continue to populate the new sweet corn Breedbase instance with SweetCAP germplasm and published phenotypic data to increase access to these data. We will also train the project group in the use of this platform. 1.3: Improve doubled-haploid breeding technology for sweet corn. Testcross seed produced during summer of 2024 (see 3. above), will be run through the ISU skNIRS machine to test and improve the algorithms for haploid sorting, which have been developed thus far. Further testcross seed production and evaluation is planned for summer 2025. During winter 2024/25, bulked NIRS evaluation will be employed to evaluate the ability of new inducer candidates to discriminate kernels with haploid versus diploid embryos in respective induction crosses, while also determining haploid induction rates (HIR). Selected candidates will advance to their respective BC2- or BC3-families in summer 2025, followed by additional NIRS (oil) and HIR evaluation winter of 2025/26, when also self-pollination/fixation of the best families will be initiated. Finally, the single kernel NIR model that is currently being used relies on kernel oil content, but this is an imperfect predictor of haploid status. We will investigate different model types to improve the kernel sorting process. In regards to overall SHGD breeding: -F1s from the 245 x A427 cross willl be induced winter 2024/25 for HMF evaluation in 2025 for a test of allelism between 245 and A427. Sweet and field corn SHGD data will be jointly analyzed to develop more powerful QTL and candidate gene identification and to determine whether field corn data is predictive for sweet corn and vice versa. Introgression of qshgd1 from A427 into 6 sweet corn backgrounds will be continued in collaboration between UFL and ISU by marker-assisted and phenotypic backcross selection, using an available qshgd1 marker or phenotyping for haploid fertility. Objective 2. Breeding and research of agronomic traits Another repetition of the experiment to determine the genetic control of resistance to tar spot Phyllachora maydis will be conducted. Irrigation will be increased in an attempt to improve infection. Insect tolerance and behavioral manipulation Continued work is planned for 3 of the 4 current field trials: 1) Genotypes from a mapping population will continue to be screened for corn silk fly resistance during spring 2025 at the UF/IFAS Everglades REC. 2) On-farm sampling to determine adult corn silk fly abundance and species composition will be repeated from winter 2025 - summer 2026, covering 18 - 24 sites on commercial sweet corn farms in southern Florida. 4) A release of marked corn silk fly adults will be conducted outside of experimental fields to monitor movement into the fields and provide estimates of the immigration rate that should be reduced by adult behavioral manipulation to reduce corn silk fly injury to sweet corn. Laboratory experiments will resume the evaluation of potential attractants and repellents (including essential oils) in fall 2024. Improving sweet corn seed germination in cold conditions. Selection and backcrossing of cold tolerance into elite sweet corn lines will be continued in. The resulting advanced lines will be evaluated in a cold tolerance field trial in the Columbia Basin in spring 2025 and used to understand the genetic nature and inheritance of cold tolerance in sweet corn germplasm. To further explore the use of skNIRS to rapidly quantify seed lot quality, samples of 100-150 sweet corn seed lots, including multiple lots of individual cultivars, will be requested from sweet corn seed companies. These will be screened using the skNIRS as well as five classical measures of seed quality (cold and warm seed germination and vigor, saturated cold germination test, electrolyte leakage, etc.) at SoDak Laboratories, using assays of the Association of Official Seed Analysts. Objective 3. Eating quality and nutritional properties In addition to publishing our scoping review findings on the cardiometabolic health outcomes of sweet corn and EDIS Extension publication, and presenting our findings at the FNCE conference, we plan to conduct a survey of a nationally representative sample of consumers on the knowledge and perceptions of the health benefits of sweet corn health benefits and purchasing intentions and behaviors, prepare a manuscript of the findings, and present a national webinar of all findings to nutrition educators. Objective 4. Functional characterization and gene editing of important loci Finish the characterization of the terpene synthase 8 mutants and isolate mutant alleles for the new CRISPR/Cas9 targets. Objective 5. Economic assessment, training, and outreach. A project to compare the quality of tray-packed (semi-shucked) corn on the cob to in-shuck corn on the cob is being planned. We will recruit 100 consumers of corn to evaluate samples from at least 2 different sources of tray-packed corn and 2 different sources of fresh, in-shuck corn at local retail markets. The corn samples will be shucked, washed, cooked and presented to the panelists to rate overall acceptability and the acceptability of other sensory characteristics of each sample. This study will provide insights into the consumer acceptability and quality of tray-packed corn available at retail. Develop a grad-level educational module using drone imagery and trait data for predictive genomics/phenomics to be taught in Spring 2025.
Impacts
What was accomplished under these goals?
Objective 1. Breeding Technologies 1.1: Build the sweet corn pan-genome and develop a DNA imputation pipeline. The use of k-mers increased GS prediction accuracy by 4%, on average, compared to accuracies using single nucleotide polymorphisms in the same population. We have also evaluated 3 different DNA imputation methods with varying levels of imputation accuracy. 1.2: Develop and employ methods for GS in public sweet corn public breeding programs. An R package called SimpleMating was developed to guide the selection of the best crosses in the breeding program, using additive and dominance GS models. We initiated the creation of a Breedbase instance for the SweetCAP project, which is now fully operational at https://clemson-sweetcorn.breedbase.org. In parallel, the use of phenomic selection using data from the skNIR was now validated in the field. 1.3: Improve doubled-haploid breeding technology for sweet corn. Combinations of promising DH lines with BHI306 - the haploid inducing line - are in generation F1 or BC1. Further backcrossing to the DH lines with high xenia effect is planned for the summer 2024 season, as well as test pollinations onto a conventional hybrid, to evaluate the xenia effect. The GWAS of spontaneous haploid genome doubling (SHGD) in the SweetCAP panel identified significant SNPs as well as 11 sweet corn lines that showed SHGD levels >50%. Objective 2. Breeding and research of agronomic traits 2.1: Disease resistance. Inbred lines previously detected to be resistant to Southern Corn Leaf Blight were crossed to elite materials from UF and UW. Those were sent for DH production in 2024. In 2023, we have also used the panel to determine the genetic control of resistance to tar spot Phyllachora maydis in three locations (Florida, Wisconsin and Michigan). 2.1B - Evaluation High plains wheat mosaic virus (HPWMoV) Research on HPWMoV was continued in collaboration with Drs. Jennifer Wilson and Erik Ohlson with the USDA ARS in Wooster, OH. Twenty-one isolates of HPWMoV were collected and used to test and validate the two sets of primer pairs used by the National Seed Health System's official test for HPWMoV. Only one of the two primer pairs detected all of the HPWMoV isolates. Concurrently, disease progression and symptomology associated with virus infection were characterized, which will facilitate scouting and rogueing of infected plants in fields, helping to reduce virus infestation of harvested seed lots. The seed transmission experiments detected seed infestation with HPWMoV in several seed lots collected from highly susceptible lines but not from lines with strong tolerance to HPWMoV. These preliminary results suggest virus tolerance may be a viable management strategy for reducing rates of seed infestation. Subsequent grow-out tests found a low (0.12%) HPWMoV seed transmission rate. Work is ongoing to produce infested seed lots from a diverse group of HPWMoV isolates to evaluate whether transmission rates vary among isolates. 2.2: Insect tolerance and behavioral manipulation. Four field experiments are being conducted to measure insect tolerance and insect behavior. 1) A field experiment is currently underway at the UF/IFAS Everglades REC in Belle Glade, FL to determine in-field corn silk fly severity in 60 genotypes selected from a mapping population. 2) Adult corn silk fly abundance and species composition were tracked across eighteen commercial sweet corn farms across the peak sweet corn growing season in South Florida in spring 2024. Preliminary data analysis identified some overall trends: a 34-fold increase in adult corn silk flies was seen from February to May, as well as an increase in the proportion of E. stigmatias (a species with reduced susceptibility to insecticides) compared to E. eluta and Chaetopsis massyla across the season. 3) A field experiment to determine corn silk fly adult abundance in sugarcane plots as affected by the application of the five potential attractants is still ongoing. 4) Lab experimentation found that directly exposing E. eluta pupae to DayGlo A-19 Horizon Blue resulted in >90% adults marked, without adversely effecting walking and flight under lab conditions. 2.3: Improving cold seed germination. Electrical conductivity (EC) was measured for each of the seed lots and a 1,000-kernel subsample for each lot was analyzed with a skNIRS to estimate kernel weight, pericarp thickness, and the amounts (and percentages) of oil, starch, sucrose, glucose, phytoglycogen, and total sugars. Average oil content was strongly positively correlated with quality related traits (seedling vigor: r = 0.80), emergence: 0.64, final vigor: 0.88, seedling uniformity: 0.88). This correlation was consistent within seedlots of each hybrid, indicating that oil content may be used to predict kernel quality variability across different genotypes. In contrast, average glucose content was negatively correlated with kernel quality traits (seedling vigor: -0.61, emergence: -0.42, final vigor: -0.91, seedling uniformity: -0.81). However, this correlation was only consistent across hybrids and not within a given hybrid, indicating a genotypic effect on both glucose and quality levels, rather than seed lot quality effect alone. EC was not as well correlated with other traits. Objective 3. Eating quality and nutritional properties The scoping review was updated in fall 2023. The results of this review are currently being prepared for a peer-reviewed manuscript and an extension publication. Additionally, a survey of consumer knowledge and perceptions of the health benefits of sweet corn health benefits and purchasing intentions and behaviors was developed and pilot-tested this summer. Objective 4. Functional characterization and gene editing of important loci 4.1: Functional characterization and gene editing The mGWAS target, terpene synthase 1 (ZmTPS1) was confirmed genetically to produce monoterpenes and the tps1 mutant was shown to impact corns interaction with fall armyworm and corn leaf aphids. Two homozygous loss of function mutants were isolated for the gene terpene synthase 8 that was identified from the metabolite GWAS on the sweetcorn diversity panel. These mutants are being characterized for volatile production and insect resistance. New CRISPR/Cas-9 lines were generated in corn for an additional GWAS target. 4.2: Identify novel combinations of sweet corn mutations to improve eating quality. This phase of our research is focused on the interactions of several of the debranching enzymes (ISA1, ISA2 and ZPU1) in the starch biosynthetic pathway. We have seen many consistent results with respect to enzyme activity when looking in both maize kernels and the yeast system which was developed previously. The yeast system contains all the maize starch synthetases, branching enzymes and specific sets of debranching enzymes. One key finding is that ISA1 and ZPU1 physically interact. In su1 mutants of maize the activity of ZPU1 is greatly reduced, but the amount of protein remains, as detected by western analysis. Thus, the presence of the ISA1 protein stabilizes ZPU1. Yeast 2 hybrid data indicates that there is a physical interaction between ISA1 and ZPU1, but not ISA2 and ZPU1. Objective 5. Economic assessment, training, and outreach. There is growing interest in the acceptability of raw corn and this study provided insights into this. We compared raw to cooked samples from 2 different corn hybrids popular in Florida with 100 consumers of sweet corn. For one of the hybrids, the raw samples were rated as highly as the cooked sample, but for the second hybrid, the cooked samples were rated higher. The major sensory characteristic that differed between raw and cooked corn was texture, with the cooked samples having higher texture ratings than raw samples. Overall, both raw samples were rated high on the 9-point hedonic scale, showing good overall acceptability of raw corn on the cob.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2024
Citation:
Williams II, M.W., Hausman, N.E., Saballos, A., Landau, C.A., Brooks, M.D., Flannery, P., Tracy. W.F., and Thompson, C.J. (2024). First report of severe tolpyralate sensitivity in corn (Zea mays) discovers a novel genetic factor conferring crop response to an herbicide. Pest Management Science 80: 1645-1653. https://doi.org/10.1002/ps.7896
- Type:
Journal Articles
Status:
Published
Year Published:
2024
Citation:
Wilson, A.R., Fiore, I.G., McCluskey, C.A., and Tracy, W.F. (2024). Genetic variation for endosperm carbohydrates and total soluble solids in shrunken2, sugary1, waxy1, and wild-type near-isogenic corn lines across three harvest dates. Crop Science 64, 1649-1665. https://doi.org/10.1002/csc2.21239
- Type:
Journal Articles
Status:
Published
Year Published:
2024
Citation:
Yactayo-Chang, J.P., Broadhead, G.T., Housler, R.J., Resende, M.F.R. Jr, Verma, K., Louis, J., Basset, G.J., Beck, J.J., and Block, A.K. (2024). Maize terpene synthase 1 impacts insect behavior via the production of monoterpene volatiles ?-myrcene and linalool. Phytochemistry 218, 113957. https://doi.org/10.1016/j.phytochem.2023.113957
- Type:
Journal Articles
Status:
Published
Year Published:
2024
Citation:
Zhang, P, Huguet-Tapia, J., Peng, Z., Liu, S., Obasa, K., Block, A. K., and White, F. F. (2024). Genome analysis and hyphal movement characterization of the hitchhiker endohyphal Enterobacter sp. from Rhizoctonia solani. Applied and Environmental Microbiology, e0224523. https://doi.org/10.1128/aem.02245-23
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Branch, C.A. and Tracy, W.F. (2023). Divergent selection for timing of vegetative phase change. Crop Science 63, 2196-2204. https://doi.org/10.1002/csc2.21016
- Type:
Journal Articles
Status:
Under Review
Year Published:
2024
Citation:
Chen, Y.-R., Frei, U.K., and L�bberstedt, T. (2024). Genomic Estimated Selection Criteria and Parental Contributions Increase Genetic Gain of Maternal Haploid Inducers in Maize. Theoretical and Applied Genetics. (submitted)
- Type:
Journal Articles
Status:
Under Review
Year Published:
2024
Citation:
Chen, Y.-R., Frei, U.K., and L�bberstedt, T. (2024). Multi-trait genomic prediction model with de novo GWAS increased the predictive ability for haploid induction ability and agronomic traits of haploid inducers in maize. Theoretical and Applied Genetics. (in revision)
- Type:
Journal Articles
Status:
Under Review
Year Published:
2024
Citation:
Branch, C., Baseggio, M., Resende, M., and Tracy W.F. (2024) The sugary enhancer1 (se1) Allele is Associated with Significant Decreases in Carotenoids and Tocotrienols in Yellow (Y1) sugary1 (su1) Sweet Corn. Journal of The American Society of Horticultural Science. (Accepted with revisions)
- Type:
Journal Articles
Status:
Published
Year Published:
2024
Citation:
Chen, Y.-R., L�bberstedt, T., and Frei, U.K. (2023). Development of doubled haploid inducer lines facilitates selection of superior haploid inducers in maize. Frontiers in Plant Science 14, 1320660. https://doi.org/10.3389/fpls.2023.1320660
- Type:
Journal Articles
Status:
Accepted
Year Published:
2024
Citation:
Coelho, I.F., Peixoto, M.A., Leach, K.A., L�bberstedt, T., Bhering, L.L., and Resende Jr., M.F.R. (2024). Optimizing a sweet corn breeding program: implementing genomic selection and doubled haploid technology. G3 (accepted)
- Type:
Journal Articles
Status:
Other
Year Published:
2024
Citation:
Dahl et al. A scoping review of sweet corn intake and cardiometabolic outcomes. (in preparation)
- Type:
Journal Articles
Status:
Published
Year Published:
2024
Citation:
Dermail, A., Mitchell, M. Foster, T., Fakude, M., Chen, Y.-R., Suriharn, K., Frei, U.K., and L�bberstedt, T. (2024). Haploid identification in maize. Frontiers in Plant Science 15, 1378421. https://doi.org/10.3389/fpls.2024.1378421
- Type:
Journal Articles
Status:
Published
Year Published:
2024
Citation:
Foster, T., Kloiber-Maitz, M., Gilles, L., Frei, U.K., Pfeffer, S., Chen, Y.-R., Dutta, S., Arun, Hufford, M., and L�bberstedt T. (2024). Fine mapping of major QTL qshgd1 for spontaneous haploid genome doubling in maize (Zea mays L.). Theoretical and Applied Genetics 137, 117. https://doi.org/10.1007/s00122-024-04615-y
- Type:
Journal Articles
Status:
Other
Year Published:
2024
Citation:
Foster, T.L., Frei, U.K., Fakude, M., Krause, M.D., Dutta, S., Tracy, W.F., Resende Jr., M.F.R., and L�bberstedt, T. (2024). Genome-wide association study of haploid male fertility in sweet corn. (in preparation)
- Type:
Book Chapters
Status:
Under Review
Year Published:
2024
Citation:
Gruening, V., L�bberstedt, T., and Frei, U.K. (2024). Doubled Haploid technology: Deriving Homozygous Lines in Maize in two generations using haploid inducers. Cold Spring Harbor Protocols; Alejandro Montenegro-Montero (ed.) (in revision)
- Type:
Journal Articles
Status:
Published
Year Published:
2024
Citation:
Khammona, K., Dermail, A., Wanchana, S., Suriharn, K., L�bberstedt, T., Thunnom, B., Poncheewin, W., Toojinda, T., Ruanjaichon, V., and Arikit, S. (2024). Accelerating haploid induction rate and haploid validation through marker-assisted selection for qhir1 and qhir8 in maize. Frontiers in Plant Science 15, 1337463. https://doi.org/10.3389/fpls.2024.1337463
- Type:
Journal Articles
Status:
Accepted
Year Published:
2024
Citation:
McCluskey, C.A., & Tracy, W.F. (2024). When data is missing by design: Maize genetic diversity experts perceptions and analysis of on-farm genetic diversity in the Upper Midwest. Plants, People, Planet. (Accepted)
- Type:
Journal Articles
Status:
Published
Year Published:
2024
Citation:
Peixoto, M.A., Coelho, I.F., Leach, K.A., Bhering, L.L., and Resende Jr., M.F.R. (2023). Simulation-based decision-making and implementation of tools in hybrid crop breeding pipelines. Crop Science 64, 110-125. https://doi.org/10.1002/csc2.21139
- Type:
Journal Articles
Status:
Published
Year Published:
2024
Citation:
Peixoto, M.A., Leach, K.A., Jarquin, D., Flannery, P., Zystro, J., Tracy, W.F., Bhering, L., and Resende, M.F.R. (2024). Utilizing genomic prediction to boost hybrid performance in a sweet corn breeding program. Frontiers in Plant Science 15, 1293307. https://doi.org/10.3389/fpls.2024.1293307
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Saldivar, E.V., Ding, Y., Poretsky, E., Bird, S., Block, A.K., Huffaker, A., and Schmelz E.A. (2023). Maize terpene synthase 8 (ZmTPS8) contributes to a complex blend of fungal-elicited antibiotics. Plants 12(5):1111. https://doi.org/10.3390/plants12051111
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Stutts, L., Latimer, S., Batyrshina, Z., Dickinson, G., Alborn, H.T., Block, A.K., and Basset, G.J. (2023). Cyanobacteria and plastids harbor strictly monofunctional naphthoquinol C-methyltransferases. Plant Cell 35(10): 3686-3696. https://doi.org/10.1093/plcell/koad202
- Type:
Journal Articles
Status:
Published
Year Published:
2024
Citation:
Tuggle, C.D., Clarke, J.L., Murdoch, B.M., Lyons, E., Scott, N.M., Benea, B., Campbell, J.D., Choudhury, S.D., Chung, H., Daigle, C.L., Dekkers, J.C.M., D�rea, J.R., Ertl, D.S., Feldman, M., Fragomeni, B.O., Fulton, J., Goddard, E., Guadagno, C.R., Hagen, D.E., Hess, A.S., Kramer, L.M., Lawrence-Dill, C.J., Lipka, A.E., L�bberstedt, T., McCarthy, F.M., McKay, S.D., Murray, S.C., Riggs, P.K., Rowan, T.N., Sheehan, M.J., Steibel, J.P., Thompson, A., Thornton, K.J., Van Tassell, C.P., and Schnable, P.S. (2024). Current Challenges and Future of Agricultural Genomes to Phenomes in the U.S. Genome Biology 25, 8. https://doi.org/10.1186/s13059-023-03155-w
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Progress 09/15/22 to 09/14/23
Outputs
Target Audience:Our primary target audience are stakeholders in the production and distribution of sweet corn. Our stakeholders include members of sweet corn breeding programs in seed companies, processors who decide on hybrids for contracts with growers, and sweet corn growers. Changes/Problems:Multiple investigators faced trouble recruiting. Specifically, the search for three post-docs in three different institutions (USDA, Clemson University and University of Florida) were delayed due to the lack of applicants. Two out of these three have now already been identified and recruited, but this issue caused delays in the research outcomes. In addition, winter nursery issues of ISU's provider in Chile (22/23) required the need to repeat some of the testcrosses in connection with high-oil inducer development summer 2023. In Wisconsin, COVID issues linger especially in the areas of travel for research purposes and especially hiring hourly works. Severe drought affected most of our field operations. We were able to access irrigation but some of the experiments will have to be repeated in 2024. What opportunities for training and professional development has the project provided?Professional development events: -Branch, C.A. (2023, August). The sugary enhancer1 (se1) allele is associated with significant decreases in the amount of lutein, zeaxanthin, and tocotrienols in yellow (Y1) sugary1 (su1) kernels . Vegetable Breeding Institute at Cornell University, Ithaca, NY. -Tracy, W.F. (2022, November). Update on Sweet CAP, Sweet corn coordinated Ag project . MidWest Food Products Association, Wisconsin Dells, WI. -Tracy, W.F. (2023, August). Wisconsin Sweet Corn Update . Vegetable Breeding Institute at Cornell University, Ithaca, NY. -Wilson, A.R. (2023, August). Breeding specialty corn . Vegetable Breeding Institute at Cornell University, Ithaca, NY. -Wilson, A.R. (2023, October). Breeding specialty corn . Breeders Showcase, Cold Spring, NY. List of conference presentations given relating to this grant (including student presentations) -Aboobucker-Mohammed, S.I. (2023, March 16-19). Parallel spindle genes restore haploid male fertility - removing a bottleneck in doubled haploid technology [Conference presentation]. 65th Annual Maize Genetics Meeting, St. Louis, MO. -Aboobucker-Mohammed S.I., Zhou, L., Frei, U.K., and Lbberstedt, T. (2023, Aug. 5-9). Parallel spindle genes restore haploid male fertility - removing a bottleneck in doubled haploid technology [Conference presentation]. American Society of Plant Biologists Meeting, Savannah, GA. -du Toit, L.J. (2023, January 17). High plains wheat mosaic virus: An emerging phytosanitary issue for corn and sweet corn seed [Conference presentation]. Annual Meeting of the Columbia Basin Vegetable Seed Association, Othello, WA. (60 people) -Foster, T. (2022, December 13) Genome-wide association for haploid male fertility in sweetcorn [Conference Presentation]. ISCDA Annual Meeting, Palm Beach, FL. -Foster, T., et al. (2023, March 16-19) Fine-mapping efforts reduced chromosomal region responsible for qshgd1 in maize [Conference presentation]. 65th Annual Maize Genetics Meeting, St. Louis, MO. -Pereira Lima, L., Beuzelin, J., and Seal, S.A. (2023, July 30 - August 2). Can corn silk fly adult distribution be manipulated for improved sweet corn field protection? [Conference presentation]. Annual Meeting of the Florida Entomological Society, Jupiter, FL. -Foster, T., et al. (2023, March 28). Fine-mapping efforts reduced chromosomal region responsible for qshgd1 in maize Maize [Poster]. R.F. Baker Symposium at Iowa State University, Ames, IA. Seminars/webinars/talks completed -Lbberstedt, T. (2023, February ) Recent advances in maize doubled haploid technology [Seminar/webinar/talk]. Khon Kaen University, Khon Kaen, Thailand. -Lbberstedt, T. (2023, March 28). Efficient breeding of resilient crops using doubled haploid technology [Seminar/webinar/talk]. R.F. Baker Symposium at Iowa State University, Ames, IA. -Resende M. Genomics applied to sweet corn breedin. Texas A&M Plant Breedin Circle, 2023. -Resende M. Integrating Genomics, Phenomics and other tools to develop better sweet corn varieties. National Association of Plant Breeders, 2023. -Resende M. Population genetics, GWAS, and breeding of sweet corn. Michigan State University, 2023. -Resende M. (2022, December 6). Sweet Breeding updates [Seminar]. UF/IFAS Sweet Corn Management Workshop, Belle Glade, FL. -Beuzelin, J. (2022, December 6). Sweet Corn Insect Ecology and Management [Seminar]. UF/IFAS Sweet Corn Management Workshop, Belle Glade, FL. -Block, A.K. (2023, September 6). Careers in ARS [Seminar]. Plant breeding graduate class at the University of Florida, Gainesville, FL. -Block A.K. (2023, March 3). Careers in ARS [Seminar]. 2023 Spring seminar series for the genetics and genomics graduate program at the University of Florida, Gainesville, FL. -Hershberger, J.M. (2023, February 17). Sowing seeds for a quality-focused vegetable breeding and genetics program [Seminar]. Annual James Brewbaker Lecture in Plant Genetics, University of Hawaii, Honolulu, HI. -Hershberger, J.M. (2023, November 11). Envisioning a quality-focused vegetable breeding program for South Carolina [Seminar]. Clemson University course AGSC 6100. Online. -Mitchell, P. (2022, November 15). Situation and Outlook for the Wisconsin Farm Economy. Wisconsin Department of Financial Institutions, Division of Banking Bank Examiner Conference, Madison, WI. Outreach: -Beuzelin, J. (2023, February 3). Keeping our growers in business: IPM in crops of the Everglades Agricultural Area [Extension Presentation]. UF/IFAS Entomology and Nematology Spring 2023 Seminar Series, Gainesville, FL. -Mitchell, P. (2023, January 30, 2023). Fresh Market Vegetable Pricing and Future Trends [Extension Presentation]. Wisconsin Fresh Fruit and Vegetable Conference, Wisconsin Dells, WI. -Mitchell, P. (2022, November 29). New Farm Bill and Crop Insurance Updates [Extension Presentation]. Processing Crops Conference, Wisconsin Dells, WI. -Information on HPWMoV was shared by Dr. du Toit with sweet corn seed industry personal and seed growers in the Columbia Basin during individual meetings with field reps and growers in 2023. -DH workshop organized by Ursula Frei, August 2-3, 2023. How have the results been disseminated to communities of interest?The results have been presented in scientific conferences and are currently being prepared for peer review publication.The results were also presented to growers association meetings in Wisconsin, Florida and Washington. Finally, the germplasm developed in the scope of this project was showcased to sweet corn seed companies and other stakeholders in field trials in Florida, New York and Wisconsin. What do you plan to do during the next reporting period to accomplish the goals?Objective 1. Breeding technologies During the next reporting period, we plan to hire and onboard a postdoctoral associate for the open position at Clemson. This individual will work with co-PI Hershberger, PD Resende, and the rest of the SweetCAP team to design and carry out experiments and analyze data related to Objective 1, primarily focusing on subobjective 1.2 (Develop and employ methods for GS in public sweet corn breeding programs). The incumbent will also work to design and implement a sustainable data management strategy for the project and greater sweet corn improvement community. 1.3: Improve doubled-haploid breeding technology for sweet corn. 1.3A - Development of a robust sorting algorithm for haploid diploid discrimination in sweet corn backgrounds In fall 2023, the individual seed, for which skNIRS data was generated, will be germinated to confirm the visual selection in haploid and diploid. Materials produced in summer 2023, which are closely related to the materials used for generating the sorting algorithm will serve for a first validation of the algorithms developed. In summer 2024, a diverse set of sweetcorn hybrids will be induced with the best haploid inducer line to test the algorithm in a broader genetic background. 1.3B - Improvement of the xenia effect in high oil haploid inducer lines, to generate a larger difference in total oil content between haploid and diploid seed In fall 2023, the oil content of all test pollinations will be determined and the Alexho derived DH lines with the best xenia effect selected. The respective F1 with the haploid inducing genotype will be used for continued haploid development in summer 2024. Additional DH lines will be increased during the winter, and tested for their xenia effect in summer 2024. 1.3C - Follow-up genetic analysis of sweet corn backgrounds with potential for SHGD Validation of the 11 high SHGD sweet corn lines, and continuation of major QTL detection and identification work. The use of those lines to introduce SHGD into sweet corn DH programs will be discussed. Moreover, PhD student Mercy Fakude will jointly analyze sweet and field corn SHGD data for more powerful QTL / candidate gene identification, and to determine, whether field corn data are predictive for sweet corn and vice versa. 1.3D - Introgression of the SHGD locus from A427 into elite sweet corn inbred lines Introgression of qshgd1 from A427 into 6 sweet corn backgrounds will be continued in collaboration between UFL and ISU by marker-assisted and phenotypic backcross selection, using an available qshgd1 marker or phenotyping for haploid fertility. Objective 2. Breeding and research of agronomic traits Objective 2.1: High plains wheat mosaic virus (HPWMoV) An isolate of HPWMoV from each of the 5-6 samples from each of ID, OR, and WA has been purified for RNA sequencing. Each isolate will be inoculated into seed of a susceptible cultivar using a vascular puncture inoculation method to propagate the strain, assess symptoms caused by each strain, evaluate the risk of seed transmission of each strain when inoculated onto the same genetic background, transferred to other corn cultivars/genetic backgrounds using the wheat curl mite vector, and inoculated onto sweet corn plants at different stages of maturity to assess how the stage of inoculation of plants impacts the risk of seed transmission. 2.2: Insect tolerance and behavioral manipulation 2.2A - GWAS and candidate genes A mapping population will be screened for insect resistance with a focus on corn silk flies during spring 2024 at the UF/IFAS Everglades REC. This screening under field conditions is expected to lead to the detection of candidate regulators of phenotypic traits associated with corn silk fly resistance. 2.2B - Population dynamics and behavioral manipulation Population dynamics. Fifteen to 20 sites on commercial farms in southern Florida will be sampled from late fall 2023 to late spring 2024 to determine corn silk fly abundance and species composition as affected by crop rotation (successive sweet corn crops vs. single sweet corn crop) and other environmental factors (e.g., sugarcane harvest). This on-farm study is expected to identify corn silk fly population trends and factors that support corn silk fly build-up and influence species composition. Behavioral manipulation. The experiment determining corn silk fly adult abundance in field plots as affected by the application of potential attractants will be repeated at the UF/IFAS Everglades REC in fall 2023. Potential repellents will be tested under laboratory and field conditions in spring and summer 2024. In addition, marked corn silk fly adults will be released outside of an experimental sweet corn field and movement into the field will be monitored, thus providing an estimate of immigration rate that should be reduced by adult behavioral manipulation to reduce corn silk fly injury to sweet corn. Objective 2.3: Improving sweet corn seed germination in cold conditions. The crosses of M-131 with elite varieties from the Univ. of Wisconsin breeding program will be planted in spring 2024 in the Columbia Basin (late March or early April, depending on weather conditions) to evaluate the selections for cold tolerance to continue integration of cold tolerance into cultivars with acceptable genetic backgrounds. We will plant multiple seed lots of varying seed quality of select commercial sweet corn cultivars under cold field conditions to continue evaluating the relationship between electrolyte leakage of sweet corn seed lots in relation to seed germination and cold tolerance. Objective 3. Eating quality and nutritional properties We plan to complete the manuscript of the scoping review findings of sweet corn consumption and cardiometabolic health benefits and submit it for publication. Additionally, we plan to draft a survey of consumer perceptions of the health benefits of sweet corn (a representative national survey is planned for fall 2024 with targeted outreach and extension activities following the survey). Objective 4. Functional characterization and gene editing of important loci Continue and complete the isolation of some of the CRISPR and transposon tagged lines and run functional analysis for insect/pest resistance traits. We also plan to fine tune the correlation between pericarp thickness and germination and use the available GWAS results to narrow additional gene candidates for gene-editing. Objective 5. Economic assessment, training, and outreach. Focus on developing a strategic assessment of the current state of the sweet corn industry in the US. Process imagery, extract traits, conduct modeling in class and release datasets. Work with industry and collaborators to identify additional computational plant science datasets and develop/release educational material along with code pipelines.
Impacts
What was accomplished under these goals?
? Objective 1. Breeding Technologies 1.1: Build the sweet corn pan-genome and develop a DNA imputation pipeline. Initial evaluation was performed for different graph-based pangenome pipelines. K-mer based GWAS was performed in the diversity population aiming at the identification of structural variation affecting traits of interest. A DNA imputation pipeline was established to evaluate the impact of low-density genotyping in sweet corn. 1.2: Develop and employ methods for GS in public sweet corn public breeding programs. Genomic selection models were calibrated using phenotypic data for multiple traits evaluated in three environments: Florida, Wisconsin and California. Spectral data management plan was designed for implementation during the project, including a sweet corn breeding database that will house project spectral and other phenotypic datasets. 1.3: Improve doubled-haploid breeding technology for sweet corn. In order to develop a robust algorithm for haploid-diploid discrimination in sweet corn backgrounds, test seed generated in summer 2021 was visually selected based on the R1-nj marker into haploid and diploid seed. Four different inducer genotypes with per se oil contents around 10-12% and an induction rate with about 9-12% were tested. For each of the twelve test combinations, more than 250 individual seeds are run through the skNIRs sorter at all three locations, to generate NIRS spectra. We initiated work with the Alexho high oil population (publicly available through GRIN) to improve the xenia effect previously identified in other high oil populations. In total 44 DH lines pollinated 3-5 ears of a conventional hybrid, to determine their xenia effect. Analysis of SHGD in the SweetCAP panel identified a total of 11 lines with SHGD levels >50%. These 11 lines will be re-induced and haploids evaluated in 2024 again for validation. A subset of five high SHGD lines was crossed with low-SHGD line Wf9, and haploids evaluated in 2023, to address the question whether major QTL are present. Also, crosses of A427 were performed with 6 sweet corn backgrounds and respective BC1 seed production was conducted at UFL. Objective 2. Breeding and research of agronomic traits 2.1: Disease resistance. -Inbred lines previously detected to be resistant to Southern Corn Leaf Blight were crossed to elite materials from UF and UW. Those will be sent for DH production in the upcoming year. -Used the panel to determine the genetic control of resistance to tar spot Phyllachora maydis. -Used the panel to study the scope of severe tolpyralate sensitivity in corn. Manuscript submitted. 2.1B - Evaluation High plains wheat mosaic virus (HPWMoV) Research was initiated with Drs. Jennifer Wilson, Virologist, and Erik Ohlson, Maize Geneticist with USDA ARS to assess the nature of sweet corn seed infection and the risk of seed transmission for infected seed lots as a result of systemic infection of mother plants vs. seed infected as a result of feeding on silks/kernels by the wheat curl mite. Samples of suspect virus-infected sweet corn crops were collected, and PCR verified to assess the genetic diversity of the virus impacting sweet corn crops in the inland Pacific Northwest. An isolate of HPWMoV from each sample has been purified for RNA sequencing, and inoculated into seed of a susceptible cultivar using a vascular puncture inoculation method to propagate each strain, assess symptoms caused by each strain, and evaluate the risk of seed transmission. This research is to be done in 2023-24. 2.2: Insect tolerance and behavioral manipulation. Laboratory and field experiments were conducted in 2023 at the UF/IFAS Everglades REC in Belle Glade, FL to evaluate the attractiveness to corn silk fly adults of five potential attractants. Two-choice cage experiments determined the preference of E. eluta and E. stigmatias females and males in two age groups, young adults (2-7 days old) and older adults (8-14 days old). In addition, a two-choice experiment using a Y-tube olfactometer characterized the behavior of E. eluta and E. stigmatias females. In these laboratory experiments, corn silk fly adults were given a binary choice between a treatment and a water control. A field experiment complemented the laboratory experiments by determining corn silk fly adult abundance in sugarcane plots as affected by the application of the five potential attractants. 2.3: Improving cold seed germination. Based on previous evaluation of the SweetCAP diversity population for cold tolerance, we have identified one line that was promising across multiple trials. This line was crossed into elite varieties from UW. In 2023, these crosses are being used for genomic selection, phenomic selection, and doubled haploid production to develop novel inbred lines that germinate well under cold conditions. In addition, to try and assess the relationship between genetic resistance or susceptibility to cold vs. the influence of seed quality on cold tolerance, a trial was initiated in late spring 2023 to establish a proof of concept of the influence of electrolyte leakage of sweet corn seed lots on cold tolerance. A sweet corn seed company provided four seed lots varying in seed quality for each of three hybrids. The goal is to subject a subsample of each seed lot to mechanical damage to increase electrolyte leakage, and then to evaluate the non-damaged and damaged seed of each lot. Objective 3. Eating quality and nutritional properties 3.1: Breeding and selection of high-quality sweet corn lines. Conducted two sensory panels at Wisconsin to evaluate the usefulness of more rapid analysis tools including BRIX and penetrometer Experiment 1 looked at elite high quality hybrids with three different endosperm types. Manuscript in preparation. Experiment 2 examined four diallel for combining ability. The four diallels consisted of isogenic sets of four different endosperm types. Manuscript submitted. 3.2: Survey to understand consumer perceptions and knowledge gaps about sweet corn's health benefits. We have undertaken a systematic scoping review of the published literature in Pubmed, Embase via Elsevier, CINAHL via EBSCO, and Science Citation Index Expanded on sweet corn consumption and cardiometabolic health benefits. Objective 4. Functional characterization and gene editing of important loci 4.1: Functional characterization and gene editing CRISPR constructs were designed to target several genes identified from the metabolite GWAS on the sweetcorn diversity panel. Loss of function mutants have been identified in 6 genes and work is ongoing to isolate homozygous mutants for functional analysis. Transposon tagged lines were identified for additional genes identified from the metabolite GWAS on the sweetcorn diversity panel and these lines were grown and are currently being screened to isolate mutants. 4.2: Identify novel combinations of sweet corn mutations to improve eating quality. Continuing on from our previous paper with forming polyglucans from maize enzymes in the yeast system, we looked specifically at the debranching enzymes when coupled with the Starch Synthases and Starch branching enzymes. Polyglucan amounts were quantified for all combinations of ISA1, ISA2 and ZPU.We then looked specifically at the enzyme activity levels of the debranching enzymes in each strain.We examined interactions using Yeast 2 Hybrid to determine if there were specific interactions between the debranching enzymes and other starch biosynthetic enzymes. Objective 5. Economic assessment, training, and outreach. In support of genetics/breeding goal and education objective, we grew a subset of the diversity panel in Michigan and collected and preprocessed drone imagery that will be used for the spring course on working with data. We also carried extension/outreach efforts to farmers and agricultural professionals regarding the current state of the sweet corn and processing vegetable industry in the state and region.
Publications
- Type:
Journal Articles
Status:
Submitted
Year Published:
2023
Citation:
Coelho, I.F., Peixoto, M.A., Leach, K.A., L�bberstedt, T., Bhering, L.L., and Resende Jr., M.F.R. (In review). Optimizing a sweet corn breeding program: implementing genomic selection and doubled haploid technology. G3 (in revision)
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Amorim et al. Simulation Based Decision Making and Implementation of Tools in Hybrid Crop Breeding Pipelines. Crop Science
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Graciano et al. Phenomic Selection Using Single Kernel NIRS for Predicting Complex Field Traits in Sweet Corn. NAPB,
2023.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Graciano et al. Exploring the Potential of Near-Infrared Spectroscopy for Predicting Complex Field Traits in Sweet Corn.
ASHS, 2023
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Amorim et al. Implementation of genomic selection in hybrid sweet corn breeding programs targeting long-term genetic
gains. NAPB, 2023
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Amorim et al. An R-package for crossing optimization in breeding programs. Oral Presentation, Corn Breeders Meeting,
2023
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Aboobucker, S.I., L�bberstedt, T. (2023) A genetic mechanism to restore haploid male fertility in Arabidopsis an alternative to chemical methods. Nature Plants, 9, 205-206. https://doi.org/10.1038/s41477-022-01335-3
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Aboobucker, S.I., Zhou, L., L�bberstedt, T. (2023) Haploid male fertility is restored by mutations in parallel spindle genes in Arabidopsis thaliana. Nature Plants, 9, 214-218. https://doi.org/10.1038/s41477-022-01332-6
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Branch, C.A., and Tracy, W.F. (2023). Divergent selection for timing of vegetative phase change. Crop Science, 63, 2196 2204. https://doi.org/10.1002/csc2.21016
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Colley, M.C., Dawson, J.C., McCluskey, C., Myers, J.R., Tracy, W.F., and Lammerts van Bueren, E.T. (2022). Exploring the emergence of participatory plant breeding in countries of the global North. The Journal of Agricultural Science, 159, 320 338. https://doi.org/10.1017/S0021859621000782
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Colley, M.C., Tracy W.F., Lammerts van Bueren, E., Diffley, M., and Almekinders, C. (2022). How the seed of participatory plant breeding found its way in the world through adaptive management. Sustainability, 14(4), 2132. https://doi.org/10.3390/su14042132
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Dermail, A., Chankaew, S., Lertrat, K., Suwarno, W.B., L�bberstedt, T., and Suriharn, K. (2023). Combining ability of tropical x temperate maize inducers for haploid induction rate, R1-nj seed set, and agronomic traits. Frontiers in Plant Science, 14, 1154905. https://doi.org/10.3389/fpls.2023.1154905
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Dong, D., Nagasubramanian, K., Wang, R., Frei, U.K., Jubery, T.Z., L�bberstedt, T., and Ganapathysubramanian, B. (2023). Self-supervised corn kernel classification and segmentation for embryo identification. Frontiers in Plant Science, 14, 1108355. https://doi.org/10.3389/fpls.2023.1108355
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Tang, H.V., Berryman, D.L., Mendoza, J., Yactayo-Chang, J.P., Li, Q.B., Christensen, S.A., Hunter, C.T., Best, N., Soubeyrand, E., Akhtar, T.A., Basset, G.J., and Block, A.K. (2022). Dedicated farnesyl diphosphate synthases circumvent isoprenoid-derived growth-defense tradeoffs in Zea mays. Plant Journal, 112, 207-220. https://doi.org/10.1111/tpj.15941
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Trentin, H.U., Krause, M., Zunjare, R., Costa Almeida, V., Rotarenco, V., Rotarenco, V., Frei, U.K., Beavis, W.D., and L�bberstedt, T. (2023). Genetic basis of maize maternal haploid induction beyond MATRILINEAL and ZmDMP U.S. Frontiers in Plant Science, 14: 1218042. https://doi.org/10.3389/fpls.2023.1218042
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Trentin, H.U., Yavuz, R., Dermail, A., Frei, U.K., Dutta, S., and L�bberstedt, T. (2023). A comparison between inbred and hybrid maize haploid inducers. Plants, 12, 1095. https://doi.org/10.3390/plants12051095
- Type:
Journal Articles
Status:
Under Review
Year Published:
2023
Citation:
Amorim et al. Utilizing genomic prediction to boost hybrid performance in a sweet corn breeding program. Frontiers in Plant Science.
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