Source: FLORIDA A&M UNIVERSITY submitted to NRP
DEVELOPMENT OF INNOVATIVE AND BIOLOGICALLY BASED INTEGRATED PEST MANAGEMENT FOR INDUSTRIAL HEMP PRODUCTION: ENHANCED COST EFFECTIVENESS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1028632
Grant No.
2022-38821-37374
Cumulative Award Amt.
$298,737.00
Proposal No.
2021-12817
Multistate No.
(N/A)
Project Start Date
May 15, 2022
Project End Date
May 14, 2026
Grant Year
2022
Program Code
[EQ]- Research Project
Recipient Organization
FLORIDA A&M UNIVERSITY
(N/A)
TALLAHASSEE,FL 32307
Performing Department
College of Agriculture & F. S
Non Technical Summary
This project is concerned with the development of an innovative integrated pest management strategy for production of industrial hemp and an evaluation of its potential economic benefits. The passage of historic legislation which removed hemp from the Controlled Substances Act has led to its present status as a multimillion-dollar industry. There are many opportunities for small- and large-scale farmers to be involved in this new and promising agricultural industry. However, because of Florida's favorable climate, pest species pose a serious threat to hemp production. Pesticides that are currently registered for use are not very effective against pests of hemp. Therefore, we intend to develop and apply a novel integrated pest management (IPM) strategy for hemp growers and evaluate its potential economic impact. The project objectives are: (a) to develop biologically based integrated pest management (BBIPM) strategies to manage pests on industrial hemp in North and Central Florida; (b) to assess the cost effectiveness of hemp production with and without the use of BBIPM. (c) to provide web-based identification tools for pests; and (d) to develop species distribution models for stakeholders. In order for new technologies and forecasting tools to reach end-users, this project will transfer technology through extension programs and provide workshops, hands-on trainings, e-certificates, experiential learning to students, extension personnel, farmers, and other stakeholders.
Animal Health Component
20%
Research Effort Categories
Basic
50%
Applied
20%
Developmental
30%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
21617301130100%
Knowledge Area
216 - Integrated Pest Management Systems;

Subject Of Investigation
1730 - Hemp;

Field Of Science
1130 - Entomology and acarology;
Goals / Objectives
The specific objectives are: 1) Develop biologically based integrated pest management (BBIPM) strategies for key pests of industrial hemp; 2) Assess the cost effectiveness of hemp production with and without BBIPM; 3) Survey, identify and develop web-based tools and distribution maps for native and invasive pest species of industrial hemp; and 4) Conduct workshops, experiential learning and hands-on trainings for farmers, students, and other stakeholders in Florida and neighboring states.
Project Methods
Objective 1. Development of Biologically Based Integrated Pest Management for Key Pests on Industrial Hemp Production. The experiments will be conducted on 92 ft by 237 ft located on sandy loam soil, well drained, with a pH of 6.5. Trap and refuge crops in perimeter cropping will include marigold and sorghum as trap crops and sunflower and candy mint as refuge crops. The experiments will be conducted in a randomized complete block design with four treatments and three replications per treatment. Treatment 1 will consist of industrial hemp only, treatment 2 will be industrial hemp with trap and refuge crops, treatment 3 will be industrial hemp with trap and refuge crops plus inoculative releases of beneficial arthropods. Treatment 4 will be industrial hemp with no trap and refuge crops, but with pesticide applications. The experimental plots for treatment 4 will be under conventional pest management with pesticides while treatments 2 and 3 will be under conservation biological control of pest species. The efficacy of the treatments will be assessed using the percent of infestations of key pests (mites, aphids, whiteflies, corn earworm, and thrips) of hemp per plant in each treatment regime from years 1-3.Data on the percentage infestations of pests and data on the numbers of beneficial arthropods collected will be analyzed using Analysis of Variance (PROC Mixed; SAS Institute, 1996).Objective 2. Benefit-Cost and Cost-effectiveness Analyses for Biologically Based IPM for Industrial Hemp Production. We will use benefit-cost and cost-effectiveness techniques, from a firm perspective, to quantify and contrast the economic impact of using conventional pest control strategies over the biologically based integrated pest management method (which is chemical-free hemp production). We will develop a 5 and 10-year investment plan using market data and a price-cost time-trend model to forecast future prices and costs of hemp production. We will examine the net present value (NPV), the benefit-cost ratio (B/C), and the internal rate of return (IRR) for these pest management strategies. The NPV is equal to the sum of discounted inflows minus the sum of discounted outflows using a predetermined discount or interest rate.Objective 3. Survey and Identifications of Pest Species of Concern to Industrial Hemp Production in Florida Each year, biweekly surveys will be conducted in our experimental plots of industrial hemp. Insects will be collected using sweep nets, sticky traps, and hand collection. Immature stages of insects will be brought in the laboratory for partial rearing to find their natural enemies. Inventory will be developed based on the levels of infestations (roots, aerial parts of the plants - leaves, branches, flowers, fruits, and seeds). Pests will be categorized into major, minor, and occasional pests as well as native or invasive species.Objective 4. Development of e-learning Certificate Program and Experiential Learning for Students, Workshops, and Hands-on Training for Stakeholders E-learning Certification. The project will develop a certification program using an e-learning platform coupled with experiential and hands-on training for farmers, students, and other stakeholders. It will consist of two certification options: a certification of participation (COP), and a certification of completion (COC). For experiential learning, each undergraduate student will develop and carry out mini-research projects. Topics may include (a) assessing the efficacy of trap and refuge crops in hemp cropping systems, (b) sampling methods of pest species on industrial hemp production, (c) assessment of the impact of beneficial insects and non-target organisms, and (d) collection and identification of pest species on industrial hemp. In addition, a MS graduate student sponsored by the project will study "Microbial Control of Key Pests of Industrial Hemp" Evaluation Plan. Research progress evaluation, implementation, and administrative monitoring of this project will be accomplished through an evaluation team (Advisory Committee). The analysis of survey questions developed by the evaluation team on the effectiveness, progress and impacts as well as the opinions and feedback from collaborators (growers), public agencies, and crop producers will be used as yardsticks for evaluation.

Progress 05/15/23 to 05/14/24

Outputs
Target Audience:We targeted several stakeholders which include students, extension agents, universities, scientific community, industrial hemp producers (small and large scale), chemical companies (insecticides, miticides, biorational compounds etc.), USDA, ARS/APHIS, Florida Department of Agriculture and Consumers Services (FDACS), pharmaceutical companies, Food and Drug Administration, medical professionals, health professionals, food, feed, fiber, public at large, pest control industry, state and federal agencies, fungal producing companies, Environmental Protection Agencies, beneficial insect producing companies, and other stakeholders. Changes/Problems:No changes are needed at this time. What opportunities for training and professional development has the project provided?Students who participated in these research projects have many opportunities for training and professional development. Each undergraduate student is required to carry out mini-research activities that were conducted in our laboratory or in laboratories of our collaborators. These research activities led to oral and poster presentations at professional meetings. In addition, each student had a faculty and a graduate student as mentors to evaluate and guide the students' projects. Graduate students received training and conducted research towards the MS degree program. In addition to the mini-research projects, we have developed seminar series every semester and featured guest speakers in entomological sciences and entrepreneurship that the students must attend. Furthermore, the undergraduate students receiving a stipend from this project were required to serve on the Student Ambassadors Club of the college and participate in the Cultivating Undergraduate Research Scholars (CURS) Program in the College of Agriculture and Food Sciences (CAFS). They also were strongly encouraged to serve as members of professional organizations (FES, ESA, etc.) and to register for the Florida A&M University (FAMU) Career Development Pathways. The FAMU REC in Quincy (where the field trials were conducted) served as a demonstration/model farm where the technology was transferred to stakeholders. The stakeholders and clientele participated each year in the "Alternative Crop Enterprise Workshop" with a view to enhancing their knowledge, skills, and abilities to correctly identify, monitor, and manage hemp insect pests. How have the results been disseminated to communities of interest?The project accomplishments were disseminated through a variety of avenues, including websites, newsletters, seminars, professional meetings, workshops, and special reports. The dissemination process interlinks with the cooperative extension program located in CAFS and the public relation service of FAMU. Additional avenues include extension bulletins, brochures; seminars; workshops; special reports of CAFS; and the CBC's Newsletters. Scientific findings are reported in referred journals (Journal of Education and Practice, Florida Entomologist, Journal of Agriculture, Journal of Environmental Entomology etc.). A web page for the project is being developed to bring more public awareness to the project and serve as an additional venue where participants at workshops can register and provide feedback. What do you plan to do during the next reporting period to accomplish the goals?We will continue the following activities (a) field implementation of biologically based IPM management of major pests of industrial hemp, (b) assessment of cost effectiveness of the IPM strategies, (c) collection and identification of pest species of concern to industrial hemp producers as well as beneficial species, and (d) student and stakeholders' experiential learning. We will continue to engage students and other stakeholders through public events and social media. Use virtual platforms to create interactive content to disseminate information and engage prospective students. We will continue to strengthen our partnerships with USDA-ARS, USDA-APHIS, FDACS, and the Florida Pest Management Association and maximize the relationship between CAFS, and other STEM colleges at the University. These strong cooperative linkages will continue to serve as useful and reliable foundations for future ventures. We will develop high-definition (Lucid keys) insect and mite images and spreadsheets to provide support to extension and outreach. We will continue to present research findings at scientific meetings to support stakeholders and clientele.

Impacts
What was accomplished under these goals? Objective 1. Development of Biologically Based Integrated Pest Management for Key Pests in Industrial Hemp Production. We designed six experimental plots and established drip irrigation in the plots. The industrial hemp seeds were sown in the greenhouse until the seedlings bear 4 leaves, they were then transferred in pot soils and held in high tunnels for a couple of weeks before they were transplanted in the fields. We used perimeter cropping consisting of a single row of 3 trap crops (marigold, bidens, and basil) followed by 3 refuge crops (sunflower, sorghum, and sweet alyssum) alternately and planted surrounding the main crop. Our monitoring and scouting data did not indicate the presence of high pest populations; thus, we did not conduct any inoculative released of natural enemies for this growing season. All insects collected during the samplings were brought to the laboratory for identification as described below. Objective 2. Benefit-Cost and Cost-effectiveness Analyses for Biologically Based IPM for Industrial Hemp Production. We collected information on any costs associated with the implementation of the industrial hemp field trials to develop a 5- year investment plan using market data and a price-cost time-trend model to forecast future prices and costs of hemp production. Our input includes (fertilizer, Top shade, electricity, seed, plastic mulch, drip irrigation systems, other consumable materials, labor, etc.) amounted to $29, 613 for this year. The average cost of hemp production of our collaborators ranged from $20,000 to $100,000 with some costs being variable based on the size and scope of the operation. Objective 3. Survey and Identifications of Pest Species of Concern to Industrial Hemp Production in Florida The insect pest species collected in our experimental plots include tobacco budworm, Cloridea virescens (Lepidoptera: Noctuidae), flea beetle, Altica sp. (Coleoptera: Chrysomelidae), lygus bug (Hemiptera: Miridae), grasshopper, Schistocera sp (Orthoptera: Acrididae), fungus gnats, Bradysa sp (Diptera: Sciaridae), leaf miner, Liriomyza sp. (Diptera: Agromyzidae), and Aphis sp. (Hemiptera: Aphididae). We also found sweet potato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), southern green stink bug, Nezara viridula (Hemiptera: Pentatomidae), brown stink bug, Euschistus servus (Hemiptera: Pentatomidae), leaf-footed bug, Leptoglossus phyllopus (Hemiptera: Coreidae), red imported fire ant, Solenopsis invicta (Hymenoptera: Formicidae), thrips, Thrips tabaci (Thysanoptera: Thripidae) and two spotted spider mite, Tetranychus urticae (Acarina: Tetranychidae). The biological control agents include Trissolcus basalis (Diptera: Tachnidae) on N. viridula; minute pirate bug, Orius insidiosus feeds on aphids, mites, and whiteflies; green lacewing, Chrysoperla carnea feeds on aphids, and Asian lady beetle, Harmonia axyridis (Coleoptera: Coccinelidae) feeds on aphids and whiteflies. We also found some spiders feeding on hemp pests, and a predatory mite, Phytoseiulus persimilis (Acarina: Phytoseiidae) in our experimental plots. In terms of diseases, we found powdery mildew, Botryti and new Leaf Spot Fungus - Bipolaris spp. fungus. Septoria leaf spot is the most common disease found in our experimental plots. This fungal leaf disease can rapidly spread throughout the fields during periods of extensive rain or high humidity. Objective 4. Development of e-learning Certificate Program and Experiential Learning for Students, Workshops, and Hands-on Training for Stakeholders In addition to the online course "ENY 2006 Global Integrated Pest Management" which includes case studies on industrial hemp Integrated Pest Management (IPM) for use in E-learning certification program. We offered the course ENY 4907 "Special Research Problems" each semester which allowed undergraduate students to carry out mini-research projects on hemp IPM. This initiative has been very popular on FAMU campus in such each semester 5-10 students from the Department of Biology and the College of Pharmacy gained knowledge and research skills through hands-on training for used in future endeavors. The students were supervised by a faculty, a postdoctoral fellow, or graduate student mentors during these experiential learning activities as described above. These students generated results from their mini-research projects and gave oral and poster presentations. The mini-research projects include (a) assessing the efficacy of trap and refuge crops in hemp cropping systems, (b) sampling methods of pest species on industrial hemp production, and (c) collection and identification of pest species on industrial hemp. We also provided summer camp activities for high school students. This initiative exposed 10-15 participants to pest management strategies (digital pest identification, biological control etc.) during two weeks between June and July. Participants created their own insect collection boxes, explored careers in entomology and investigated edible insects. In addition, a graduate student is conducting his research activities towards the fulfillment of the requirements for the Master of Science (MS) degree in entomology. The title of his research is "Microbial Control of Key Pests of Industrial Hemp."

Publications

  • Type: Conference Papers and Presentations Status: Other Year Published: 2024 Citation: Bramwell G., and L. H. B. Kanga. 2024. Development of a Biological-Based Integrated Pest Management Strategies for Key Pests on Industrial Hemp (Cannabis Sativa L) Production in Florida., held in Tallahassee, Florida.


Progress 05/15/22 to 05/14/23

Outputs
Target Audience:We targeted several stakeholders which include industrial hemp producers (small and large scale), chemical companies (insecticides, miticides, biorational compounds etc.), USDA, Florida Department of Agriculture and Consumers Services (FDACS), pharmaceutical companies, Food and Drug Administration, medical professionals, health professionals, food, feed, fiber, public at large, pest control industry, state and federal agencies, fungal producing companies, Environmental Protection Agencies, beneficial insect producing companies, universities, scientific community, students, extension agents and other stakeholders. Changes/Problems:No changes are needed at this time. What opportunities for training and professional development has the project provided? Several undergraduate and graduate students who participated in these research projects have many opportunities for training and professional development. Each undergraduate student who is granted stipends under this project is required to carry out mini-research activities that were conducted in our laboratory or in laboratories of our collaborators. These research activities led to oral and poster presentations at professional meetings. In addition, each student had a faculty and a graduate student as mentors to evaluate and guide the students' projects. Graduate students received training and conducted research towards the MS degree program. A career development seminar series was given every three months and featured guest speakers in entomological sciences and entrepreneurship that the students must attend. Furthermore, the undergraduate students were required to serve on the Student Ambassadors Club of the college and participate in the Cultivating Undergraduate Research Scholars (CURS) Program in the College of Agriculture and Food Sciences (CAFS). They also were strongly encouraged to serve as members of professional organizations (FES, ESA, etc.) and to register for the Florida A&M University (FAMU) Career Development Pathways. The project developed a certification program using an e-learning platform coupled with experiential and hands-on training for farmers, students, and other stakeholders. The FAMU REC in Quincy (where the field trials were conducted) served as a demonstration/model farm where the technology was transferred to stakeholders. The stakeholders and clientele have participated in the "Alternative Crop Enterprise Workshop" with a view to enhancing their knowledge, skills, and abilities to correctly identify, monitor, and manage hemp insect pests. How have the results been disseminated to communities of interest?The project accomplishments were disseminated through a variety of avenues, including websites and newsletters. Project achievements were immediately available to the industry through well-established technology transfer routes involving the Extension Service located in CAFS. Additional avenues include extension bulletins, brochures; seminars; workshops; special reports of CAFS; and the CBC's Newsletters. A web page for the project is being developed to bring more public awareness to the project and serve as an additional venue where participants at workshops can register and provide feedback What do you plan to do during the next reporting period to accomplish the goals?We will continue the following activities (a) field implementation of biologically based IPM management of major pests of industrial hemp, (b) assessment of cost effectiveness of the IPM strategies, (c) collection and identification of pest species of concern to industrial hemp producers as well as beneficial species, and (d) student and stakeholders' experiential learning. We will continue to engage students and other stakeholders through public events and social media. Use virtual platforms to create interactive content to disseminate information and engage prospective students. We will continue to strengthen our partnerships with USDA-ARS, USDA-APHIS, FDACS, and the Florida Pest Management Association and maximize the relationship between CAFS, and other STEM colleges at the University. These strong cooperative linkages will continue to serve as useful and reliable foundations for future ventures. We will develop high-definition insect and mite images and spreadsheets to provide support to extension and outreach. We will develop commodity-based information sheets on the major insect and mite pests and their biological control agents. We will continue to present research findings at scientific meetings to support stakeholders and clientele

Impacts
What was accomplished under these goals? Objective 1. Development of Biologically Based Integrated Pest Management for Key Pests in Industrial Hemp Production. We designed three experimental plots (plot 1 has 10,032 sq ft; plot 2 has 17,458 sq ft, and plot 3 covers 19,596 sq ft) to conduct our field trials at the FAMU Research and Extension Center in Quincy, FL. The industrial hemp seeds were purchased from Johnny's Seed (Ft. Myers, FL), and Harris Seeds (Rochester, NY) and they were sown in the nursery before transplanting them in the fields. We established drip irrigation for all our experimental plots. All crops were planted on raised beds covered with a black fabric to reduce plant diseases and weed control. We used perimeter cropping with trap crops (marigold), and refuge crops (sunflower) planted surrounding the main crop. Our field scouting data did not indicate the presence of large pest populations; thus, we did not conduct any inoculative released of natural enemies for this growing season. All insects collected during the samplings were brought to the laboratory for identification as described below. Objective 2. Benefit-Cost and Cost-effectiveness Analyses for Biologically Based IPM for Industrial Hemp Production. We collected input and yield data from our pest management experiments along with all pest control activities and associated costs. We are developing a 5- year investment plan using market data and a price-cost time-trend model to forecast future prices and costs of hemp production. Our input includes (fertilizer, electricity, seed, fabric mulch, drip irrigation systems, other consumable materials, labor, etc.) and amounted to $19, 000 for this year. The average cost of hemp production of our collaborators ranged from $20,000 to $100,000 with some costs being variable based on the size and scope of the operation. Objective 3. Survey and Identifications of Pest Species of Concern to Industrial Hemp Production in Florida Several species of insect and mite pests and their biological control agents were found on our industrial hemp experimental plots; thus, we developed a listing of these organisms. The insect pest species include sweet potato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), southern green stink bug, Nezara viridula (Hemiptera: Pentatomidae), brown stink bug, Euschistus servus (Hemiptera: Pentatomidae), leaffooted bug, Leptoglossus phyllopus (Hemiptera: Coreidae), red imported fire ant, Solenopsis invicta (Hymenoptera: Formicidae), and one unidentified species of a caterpillar and a leaf miner that feeds on the leaves of hemp was collected. In addition, aphids and thrips were occasionally found. The biological control agents include Trissolcus basalis (Diptera: Tachnidae) on N. viridula; minute pirate bug, Orius insidiosus feeds on aphids (Fig. 1 A), mites, whiteflies; green lacewing, Chrysoperla carnea feeds on aphids, and Asian lady beetle, Harmonia axyridis (Coleoptera: Coccinelidae) (Figs. D and C) feeds on aphids and whiteflies. We also found some spiders feeding on pest species. In addition, one species of a plant-feeding mite (two spotted spider mite), Tetranychus urticae (Acarina: Tetranychidae) and one species of a predatory mite, Phytoseiulus persimilis (Acarina: Phytoseiidae) (Fig 2. F) were also found in our experimental plots. A B C Figure 1 (A) Stink bug with pirate bug eggs, (B) Caterpillar, (C) Red spider mite C D E Figure 2. Larvae of ladybird beetle (D) and (E) and (F) predatory mite (P. persimilis) ( In terms of diseases, we found powdery mildew, Botryti and new Leaf Spot Fungus - Bipolaris spp. fungus. Objective 4. Development of e-learning Certificate Program and Experiential Learning for Students, Workshops, and Hands-on Training for Stakeholders We have developed an online course "ENY 2006 Global Integrated Pest Management". This course includes case studies on industrial hemp IPM and production for use in E-learning certification program. The course will be accessible during the Spring semester of 2024. Three undergraduate students have conducted mini-research projects on (a) assessing the efficacy of trap and refuge crops in hemp cropping systems, (b) sampling methods of pest species on industrial hemp production, and (c) collection and identification of pest species on industrial hemp. In addition, a graduate student is conducting his research activities towards the fulfillment of the requirements for the Master of Science (MS) degree in entomology. The title of his research is "Microbial Control of Key Pests of Industrial Hemp."

Publications

  • Type: Conference Papers and Presentations Status: Submitted Year Published: 2023 Citation: Haseeb, M. and L.H. B. Kanga. 2023. Integrated pest management and plant health. Talk presented during the workshop on the alternative crop enterprise, held in Brooksville, Florida.