Progress 05/01/24 to 04/30/25
Outputs Target Audience:The graduate and undergraduate students, post-doctorate research scientist, lab technician, extension agents, farmers are the target audience for this project. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?During the execution of this project, the graduate and undergraduate students, lab technician, post-doctorate, extension agents were trained with better skills and knowledge related with plant tissue culture techniques, crop genetic engineering and gene transformation, disease diagnosis techniques and field practices. Three courses related with tissue culture and genetic engineering techniques were well developed at undergraduate and graduate class levels. All the personnel mentioned above are benefit for the training and professional development. How have the results been disseminated to communities of interest?The research data were presented to several scientific meetings, including the annual Mississippi Academy of Sciences Meeting at Biloxi, MS; Two graduate students presented their research findings and got the divisional first and second places for graduate students' oral presentation. What do you plan to do during the next reporting period to accomplish the goals?Our next goals to be accomplished will be objectives 2 and 4. Especially, to examine the transgenic lines in sweetpotato with enhanced protein levels for their viral resistance. CRISPR/Cas9 targeted mutagenesis for overexpression of sweetpotato NF-YC4 will be developed to increase protein levels and disease resistance in sweetpotato roots. PI, Co-PIs and collaborators will continue to arrange meetings to discuss plans for execution of the goals.
Impacts What was accomplished under these goals?
We have been focusing on objective 1 and 3 in year 3: to generate Arabidopsis QQS and sweetpotato NF-YC4 transgenic sweetpotato to increase protein levels and disease resistance in sweetpotato roots via Agrobacterium-mediated transformation method. To fulfill this goal, we optimized protocols to generate transgenic sweetpotato lines expressing the QQS gene and overexpressing IbNF-YC4. The QQS gene was cloned from Arabidopsis thaliana ecotype Columbia 0 (Col-0) and sweetpotato NF-YC4 was cloned from variety PI566648 (Red Jewel). Expression vectors for Arabidopsis QQS and sweetpotato NF-YC4 were constructed and transformed into Agrobacterium tumefaciens strain EHA105. We optimized the regeneration and Agrobacterium-mediated gene transformation systems for sweetpotato, conducting glufosinate concentration tests for selection in line Red Jewel. Over 15 glufosinate-resistant plants were generated from each transformation construct, and PCR analysis confirmed successful gene insertion in more than 90% of regenerated plants. Quantification of protein and starch levels in transgenic sweetpotato leaves showed a significant increase in protein content and a reduction in starch accumulation compared to wild-type controls, indicating the potential for improved nutritional value in transgenic sweetpotato lines.
Publications
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Lei Wang, Carol M. Foster, Wieslawa I. Mentzen, Rezwan Tanvir, Yan Meng, Tracie Hennen- 6 Bierwagen, Basil J. Nikolau, Dan Nettleton, Alan M. Myers, and Eve Syrkin Wurtele, Ling Li (2024). Modulation of the Arabidopsis starch metabolic network by the cytosolic acetyl-CoA pathway in the context of the diurnal illumination cycle. International Journal of Molecular Sciences. 25 (19).
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Justin George, Gadi V.P. Reddy, Philip Wadl, William Rutter, Julianna Culbreath, Lau Pierre, Tahir Rashid, Matthew C. Allan, Suzanne D. Johaningsmeier, Amanda Nelson, Ming Li Wang, Augustine Gubba, Kai-Shu Ling, Yan Meng, Daniel Collins, Sathish Ponniah, Prasanna H. Gowda (2024). Sustainable Sweetpotato Production in the United States: Current Status, Challenges, and Solutions. Agronomy Journal. P.1-31
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2025
Citation:
Ramtin Vamenani, Yan Meng, Ling Li (2025). Enhancing Nutritional Content in Sweet Potato through the Orphan Gene QQS: A Metabolic Engineering Approach. 89th Annual Mississippi Academy of Sciences Meeting, Biloxi, Mississippi.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2025
Citation:
John Jones, Tatyana Hollingbird, Trinity Dailey, Lei Wang, Ramtin Vamenani, Ling Li, Yan Meng (2025). Using Agrobacterium-mediated gGene transformation approach to develop sweetpotato germplasm with enhanced protein content. 89th Annual Mississippi Academy of Sciences Meeting, Biloxi, Mississippi.
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Progress 05/01/23 to 04/30/24
Outputs Target Audience:The graduate and undergraduate students, post-doctorate research scientist, lab technician, extension agents, farmers are the target audience for this project. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?During the execution of this project, the graduate and undergraduate students, lab technician, post-doctorate, extension agents were trained with better skills and knowledge related with plant tissue culture techniques, crop genetic engineering and gene transformation, disease diagnosis techniques and field practices. Three courses related with tissue culture and genetic engineering techniques were well developed at undergraduate and graduate class levels. All the personnel mentioned above are benefit for the training and professional development. How have the results been disseminated to communities of interest?The research data were presented to several scientific meetings, including the annual Mississippi Academy of Sciences Meeting at Hattiesburg, MS, the 18th 1890 Association of Research Directors (ARD) Research Symposium at Nashville, TN, and the 10th annual Center of Research Symposium at Alcorn State University What do you plan to do during the next reporting period to accomplish the goals?Our next goals to be accomplished will be objectives 2 and 3. Especially, to test transgenic lines in sweetpotato with enhanced protein levels and viral resistance. CRISPR/Cas9 targeted mutagenesis for overexpression of sweetpotato NF-YC4 will be developed to increase protein levels and disease resistance in sweetpotato roots. PI, Co-PIs and collaborators will continue to arrange meetings to discuss plans for execution of the goals.
Impacts What was accomplished under these goals?
We have been focusing on objective 1 in year 2: to generate Arabidopsis QQS and sweetpotato NF-YC4 transgenic sweetpotato to increase protein levels and disease resistance in sweetpotato roots via Agrobacterium-mediated transformation method. Arabidopsis QQS expressing vectors were constructed. QQS was cloned from Arabidopsis thaliana ecotype Columbia 0 (Col-0). The vector was transformed into Agrobacterium tumefaciens strain EHA105. A high-efficient sweetpotato regeneration system and Agrobacterium-mediated gene transformation system for sweetpotato 'Red Jewel" cultivar was well-developed. Preliminary results showed that expression of foreign genes in kanamycin resistant plants was achieved using A. tumefaciens strain EHA105 harboring the expression cassette. The PCR result showed that the foreign QQS gene has been transformed into the sweetpotato line PI566648 (Red Jewel).
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2024
Citation:
1. Justin George, Gadi V.P. Reddy, Philip Wadl, William Rutter, Julianna Culbreath, Lau Pierre, Tahir Rashid, Matthew C. Allan, Suzanne D. Johaningsmeier, Amanda Nelson, Ming Li Wang, Augustine Gubba, Kai-Shu Ling, Yan Meng, Daniel Collins, Sathish Ponniah, Prasanna H. Gowda (2024). Sustainable Sweetpotato Production in the United States: Current Status, Challenges, and Solutions. Agronomy Journal. P.1-31 DOI: 10.1002/agj2.21539
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2024
Citation:
1. Alpha Jones, Chunquan Zhang and Yan Meng (2024). Developing efficient biotechnological approaches for sweetpotato virus detection and removal. Association of 1890 Research Directors Research Symposium 2024, Nashville, Tennessee
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2024
Citation:
2. Tatyana Hollingbird, Chunquan Zhang, Lei Wang, Ling Li and Yan Meng (2024). Developing sweetpotato germplasm with enhanced performances by using Agrobacterium-mediated transformation. Association of 1890 Research Directors Research Symposium 2024, Nashville, Tennessee
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2024
Citation:
3. Alpha Jones, Chunquan Zhang and Yan Meng (2024). Developing efficient biotechnological approaches for sweetpotato virus detection and removal. 10th Annual Center for Research Excellence Symposium, Alcorn State University, Lorman, Mississippi
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2024
Citation:
4. Alpha Jones, Chunquan Zhang and Yan Meng (2024). Developing efficient biotechnological approaches for sweetpotato virus detection and removal. 88th Annual Mississippi Academy of Sciences Meeting, Hattiesburg, Mississippi
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2024
Citation:
5. Tatyana Hollingbird, Chunquan Zhang, Lei Wang, Ling Li and Yan Meng (2024). Developing sweetpotato germplasm with enhanced performances by using Agrobacterium-mediated transformation. 88th Annual Mississippi Academy of Sciences Meeting, Hattiesburg, Mississippi
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2024
Citation:
6. Alpha Jones, Chunquan Zhang and Yan Meng (2024). Developing efficient biotechnological approaches for sweetpotato virus detection and removal. 88th Annual Mississippi Academy of Sciences Meeting, Hattiesburg, Mississippi
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2024
Citation:
7. Alpha Jones, Chunquan Zhang and Yan Meng (2024). Developing efficient biotechnological approaches for sweetpotato virus detection and removal. 10th Annual Center for Research Excellence Symposium, Alcorn State University, Lorman, Mississippi
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2024
Citation:
8. Tatyana Hollingbird, Chunquan Zhang, Lei Wang, Ling Li and Yan Meng (2024). Developing sweetpotato germplasm with enhanced performances by using Agrobacterium-mediated transformation. 10th Annual Center for Research Excellence Symposium, Alcorn State University, Lorman, Mississippi
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2024
Citation:
9. Alpha Jones, Chunquan Zhang and Yan Meng (2024). Developing efficient biotechnological approaches for sweetpotato virus detection and removal. 10th Annual Center for Research Excellence Symposium, Alcorn State University, Lorman, Mississippi
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Progress 05/01/22 to 04/30/23
Outputs Target Audience:The graduate and undergraduate students, post-doctorate research scientist, lab technician, extension agents, farmers are the target audience for this project. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?During the execution of this project, the graduate and undergraduate students, lab technician, post-doctorate, extension agents were trained with better skills and knowledge related with plant tissue culture techniques, crop genetic engineering and gene transformation, disease diagnosis techniques and field practices. Three courses related with tissue culture and genetic engineering techniques were well developed at undergraduate and graduate class levels. All the personnel mentioned above are benefit for the training and professional development. How have the results been disseminated to communities of interest?The research data were presented to several scientific meetings, including the annual Mississippi Academy of Sciences Meeting at Biloxi, MS and the 9th annual Center of Research Symposium at Alcorn State University. What do you plan to do during the next reporting period to accomplish the goals?Our next goals to be accomplished will be objectives 2 and 3. Especially, to create transgenic lines in sweetpotato with enhanced protein levels and viral resistance. CRISPR/Cas9 targeted mutagenesis for overexpression of sweetpotato NF-YC4 will be developed to increase protein levels and disease resistance in sweetpotato roots. PI, Co-PIs and collaborators will continue to arrange meetings to discuss plans for execution of the goals
Impacts What was accomplished under these goals?
We have been focusing on objective 1 in year 1: to generate Arabidopsis QQS and sweetpotato NF-YC4 transgenic sweetpotato to increase protein levels and disease resistance in sweetpotato roots via Agrobacterium-mediated transformation method. The constructs containing Arabidopsis QQS and sweetpotato NF-YC4 genes were constructed. An Agrobacterium-mediated transformation protocol was well-developed in the lab; an optimized regeneration protocol for line PI566648 was also developed. Selection antibiotic-resistant calli were obtained from sweetpotato line PI566648, we are inducing the regenerated transgenic plants now.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Lei Wang, Andrew J. Tonsager, Wenguang Zheng, Yingjun Wang, Dan Stessman, Wei
Fang, Kenna E. Stenback, Alexis Campbell, Rezwan Tanvir, Jinjiang Zhang, Samuel, Cothron, Dongli Wan, Yan Meng, Martin H. Spalding, Basil J. Nikolau, Ling Li (2023). Single-cell genetic models to evaluate orphan gene function: the case of QQS regulating carbon and nitrogen allocation. Frontiers. Frontiers in Plant Science. 14:1126139. doi: 10.3389/fpls.2023.1126139
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Favour Afolabi, Yan Meng and Ling Li (2022). Utilizing novel biotechnology to develop new sweetpotato varieties with improved protein levels and broad-spectrum disease resistance. 8th Annual Center for Research Excellence Symposium, Lorman, Mississippi. Alcorn State University, Lorman, Mississippi
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Tymesha Nabors, Chunquan Zhang and Yan Meng (2022). Developing Agrobacterium-mediated transformation approach to improve the viral resistance in sweetpotato ((Ipomoea batatas L.). 8th Annual Center for Research Excellence Symposium, Alcorn State University, Lorman, Mississippi
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Yan Meng, Tymesha Nabors, Favour Afolabi and Chunquan Zhang (2022). Developing Agrobacterium-mediated transformation approach to improve the viral resistance and protein levels in sweetpotato (Ipomoea batatas L.). 86th Annual Mississippi Academy of Sciences Meeting, Biloxi, Mississippi
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Tatyana Hollingbird, Chunquan Zhang and Yan Meng (2023). Developing sweetpotato germplasms with increased protein levels and improved viral disease resistance. 87th Annual Mississippi Academy of Sciences Meeting, Biloxi, Mississippi
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Jacob Piazza and Yan Meng (2023). Optimization of plant regeneration protocol for selective elite lines in sweetpotato (ipomoea batatas (l.) Lam). 87th Annual Mississippi Academy of Sciences Meeting, Biloxi, Mississippi
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Alpha Jones, Chunquan Zhang and Yan Meng (2023). Using meristem-tip tissue culture technique to remove viruses from sweetpotato. 87th Annual Mississippi Academy of Sciences Meeting, Biloxi, Mississippi
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