Source: DELAWARE STATE UNIVERSITY submitted to NRP
ESTIMATION OF NUTRIENT COMPOSITION AND SERVING SIZES OF AFRICAN FOODS CONSUMED IN THE U.S. TO ASSESS DIET QUALITY AND TO PROMOTE HEALTHFUL FOOD CHOICES
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1028526
Grant No.
2022-38821-37296
Cumulative Award Amt.
$599,997.00
Proposal No.
2021-12910
Multistate No.
(N/A)
Project Start Date
May 15, 2022
Project End Date
May 14, 2026
Grant Year
2022
Program Code
[EWE]- Extension Project
Recipient Organization
DELAWARE STATE UNIVERSITY
1200 NORTH DUPONT HIGHWAY
DOVER,DE 19901
Performing Department
Human Ecology
Non Technical Summary
Obesity and type II diabetes are significant public health challenges, affecting not only Americans, but many immigrant populations. Epidemiological data revealed that rising levels of obesity and diabetes among African-born immigrants contribute to rising healthcare costs. Diet is a significant, modifiable risk factor associated with obesity and type II diabetes and plays a crucial role in weight control and management of diabetes. Diet is an integral tool management of diseases, from prevention to treatment. Food analysis of indigenous foods is essential to assess dietary quality and develop nutrient intake recommendations and dietary patterns with indigenous foods based on USDA dietary guidelines. This project aims to assess dietary patterns of African ethnic foods using methodologies developed by the USDA and National Cancer Institute to assess diet quality based on the 2020-2025 Dietary Guidelines for Americans. This is an important project because current knowledge on ethnic foods consumed by Africans living in the USA is limited. Project objectives include: 1) Assess African born immigrant community perception of healthy eating habits and exercise, 2) Conduct nutrient analysis of identified staple foods and determine the recommended portion size for the selected immigrant staple foods and 3) Develop training/educational materials in collaboration with the community. Proposed Food Pattern Equivalents and African recipe collection/cookbook will assist extension specialists, hospital food service departments and health professionals to guide African-born immigrants in making healthy dietary choices and serve as the research-based foundation for nutrition policies and programs across the US
Animal Health Component
90%
Research Effort Categories
Basic
10%
Applied
90%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
7011099200050%
7021455200020%
7031099101030%
Goals / Objectives
The goalsof this project are to(i)develop integrated extension, research, and education activities to build capacity in food chemistry and food analysis, and (ii) developFood Patterns equivalents to fill a missing nutrition knowledge gap that willprovide dietitians, extension specialists and healthcare professionals with new knowledge needed in the management of weight and diabetes among the growing population of African-born immigrants. Project outcomes will be achieved by executing the extension, research, and education objectives outlined below:Assess African-born immigrant community perceptions of healthy eating habits Conduct diet analysis of the identified staple foods and determine the recommended portion size for the selected immigrant staple foodsDevelop Training/Educational materials in collaboration with the community-community engagement
Project Methods
PDs will organize focus group meetings and workshops via Zoom/Microsoft Team platforms to identify overweight/obesity and diabetes management practices among a sample of African-born immigrants. The data from the focus groups will also be utilized in the development of the data collection questionnaires. To help understand the knowledge base of the target audience, a nutrition knowledge questionnaire tested and validated as described by Towler and Shepherd (1990) and will be used to measure awareness and nutrition knowledge. The questionnaire will be structured to gather information on: (1) socio-demographics, (2) food purchase and shopping behavior, (3) food preference, and (4) consumption behavior. The project will use a Food Frequency questionnaire (FFQ) to collect data on the types and amounts of foods and beverages consumed by African-born immigrants. A detailed FFQ and a Diet History Questionnaire (DHQ) tailored to the targeted population will be developed to collect further information such as food types, quantity, dietary patterns, and mealtime. PDs plan to collect staple foods from three regions in Africa (East, West, and South) for analysis of food composition. Frozen food samples will be collected from participants during focus group meeting and placed in dry ice and transported to the DSU/TU food chemistry laboratory for storage at -20OC and analysis. PDs plan to collect food samples from at least 200 diverse groups of African families.Analysis of nutrients: Samples of cooked meals collected from participants in Delaware, and 3rd congressional district in Alabama and stored at -20? C will be analyzed in the Food Chemistry Laboratory at DSU and TU. Food samples will be analyzed for their nutrient content using standard assay methods (Association of Official Analytical Chemists, 1995, Phillips et al, 2014, Pehrsson et al. 2013). A complete proximate evaluation analysis will be conducted for (1) dry matter (DM) content; (2) crude protein (CP); (3) lipids (fats); (4) ash; (5) Insoluble dietary fiber and soluble dietary fiber, and (6) energy. Protein, total fat, TDF, total sugar, saturated fat, mono-unsaturated fat, poly-unsaturated fat, trans fat will be determined as described by Haytowitz and Pehrsson (2016). Insoluble dietary fiber (IDF) and soluble dietary fiber (SDF) will be determined by the AOAC Method 991.43 (AOAC, 1997). Total starch will be determined according to the AOAC Method 996.11 (AOAC, 1997). Iron, sodium, potassium, calcium and phosphorus will be measured using the AAS model ISO, 9001 (Haytowwitz and Pehrsson, 2016), and Vitamin A (β- carotene) and folic acid will be determined using HPLC, and vitamin D will be determined as described by Phillips et al (2018).PD plan to develop dietary patterns with cultural food staples based on the Dietary Guidelines for Americans. The dietary recommendations in the Dietary Guidelines are applicable to dietary patterns of immigrant populations including those from the African continent and the Caribbean region. PDs plan to use food analyses data (objective 4) to develop Food Pattern Equivalents (serving sizes) of selected African foods commonly eaten by African-born immigrants (collected from focus group participants). In addition, PDs plan to compute Food Patterns equivalents for each African food using USDA Agricultural Research Service's methodology (Bowman et al. 2018). The methodology includes: 1) Disaggregation of foods to its ingredients; 2) Assignment of appropriate Food Patterns (FP) components to ingredients; 3) Computing of Food Pattern equivalents per 100 grams of ingredient by applying equivalent weights; and 4) Determination of the amounts of each of the FP equivalents present per 100 grams of foods, by applying Food Pattern Ingredient Database (FPID) values.PDs plan to develop service-learning activities for Nutritional Sciences students at DSU and TU to intern/shadow a dietitian and/or extension nutrition specialist for ten weeks. Students recruited for the project will be required to register for a 1 credit hour Nutrition Education Practicum course, requiring hours of service learning with a dietitian/extension specialist. Students will engage in activities such as developing and modifying recipes, developing new food products that are culturally tailored for African-born immigrants, and developing nutrition education materials for target populations from different areas of the African continent. This project will offer students an opportunity to interact with the professionals in their field of studies and gain knowledge, and competencies required for entry-level jobs in the field of Food and Nutrition/Dietetics.PDs plan to develop special training workshops for EFNEP educators on the diet quality and sample size of immigrant food, and disease prevention. Organize food demonstration session with EFNEP audiences. In addition, webinars will be organized on diet quality of immigrant food and disease prevention.)PDs plan to collect recipes of typically consumed meals from participants and develop an African recipe manual/cookbook that will be posted on DSU's and TU's Cooperative Extension websites. The African recipe manual/cookbook will be distributed to food service departments in hospitals and nursing homes cafeteria to assist in meeting the needs of patients of African-born immigrant.Data collected from objectives 1 and 2 will be used to guide the formulation of a culturally-sensitive nutrition education handbook for cooperative extension programs on common foods, dietary practices and food patterns among African immigrants. Evidenced-basedinformation will also be provided on nutrition assessment and healthy dietary practices to assist extension personnel (EFNEP and SNAP-Ed) in providing appropriate nutrition education and plan nutrition programs addressing the needs of this community.

Progress 05/15/24 to 05/14/25

Outputs
Target Audience:African-born immigrants in the United States Graduate Students Undergraduate Students Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?We have shared project results with DSU-Cooperative Extension Specialists. We have attended community meetings organized by the target audience and shared project results with the target population What do you plan to do during the next reporting period to accomplish the goals?We plan to continuediet analysis of the identified staple foods and determine the recommended portion size for the selected immigrant staple foods and continue to develop Training/Educational materials in collaboration with the community-community engagement. The graduate student working on the project will analyze result data and write a thesis and manuscript for publication. We plan to develop food patterns equivalents to fill a missing nutrition knowledge gap that will provide dietitians, Extension specialists and healthcare professionals with new knowledge needed in the management of weight and diabetes among the growing population of African-born immigrants.

Impacts
What was accomplished under these goals? African-born immigrants (ABI) in the United States are the least-studied immigrant group. Obesity, hypertension, cardiovascular diseases, and type II diabetes are significant public health challenges, affects many immigrant populations. Lack of knowledge of food portion sizes has been reported as one of the barriers in the management of obesity and diabetes among ABI. Epidemiological data revealed that rising levels of obesity and diabetes among ABI contribute to rising healthcare costs. Diet is a significant, modifiable risk factor associated with obesity and type II diabetes and plays a crucial role in weight control and management of diabetes, hypertension, and cardiovascular diseases. The PDs organized a meeting after the Award letter was received to review project activities and project timeline. The PDs developed a job description and announcement for a post-doctoral research associate position. The PDs screened, interviewed, and hired a candidate for the post-doctoral position. The PDs have developed a thirty-question questionnaire to assess the perceptions of healthy eating habits among ABI. The questionnaire will be pre-tested in the Introduction to Nutrition Class prior to administration to target population. A letter of Consent, Protocol and IRB application has been completed for submission to DSU's IRB for approval prior to conducting the survey. The project aims to: 1) assess dietary habits among ABI in the United States, 2) develop and promote culturally tailored education programs to support healthy eating behaviors, and 3) develop a train-the-trainer program through formal and informal education programs. To assess dietary habits among ABI, a thirty-two-nutrition knowledge questionnaire tested and validated was used to measure awareness and nutrition knowledge. The questionnaire was structured to gather information on: (1) socio-demographics, (2) food preference, and (3) consumption behavior. The questionnaire was administered to 300 participants within the target population electronically via SurveyMonkey. A total of 200 participants completed the survey. Data collected and analyzed in Microsoft Excel, and results were expressed in percentages. Males (53%) constituted most of the study participants surveyed, and most of the participants (56%) were older than 51 years and have completed BS degree programs. About 79% of these participants have full-time employment. The study revealed that most (70%) participants have lived in the US more than 10 years. Twenty percent of participants were diagnosed with diabetes, and 68% of this group reported that they have Type 2 diabetes, 24% have type 1 diabetes and 8% have gestational diabetes. The majority (91%) of the participants are not aware and have never participated in a diabetes education program. Forty-four percent (44%) of participants reported that they were diagnosed with high blood pressure and 64% reported that they gained body weight since immigrated to the US. Based on our assessment of eating behavior, study participants consume mostly (47%) food from country of birth and some American food and eat more (73%) home prepared meals. Some (42%) participants could not identify low glycemic index foods from foods they frequently consume. Most (60%) participants strongly agree that if they know the composition of their staple foods it would help them make healthful food choices. About 84% of participants reported that olive oil a healthier cooking oil than other commercial oils. We found that the study participants consume more (59%) of vitamin D and more (39%) calcium. Results from this study will assist in the development of culturally and contextually appropriate approaches to tackle food and nutrition insecurity and prevent and control diet-associated chronic diseases and health disparities in this community. To estimate the nutrient composition and create an extensive nutrient composition database for staple African foods prevalent among African-born immigrants, we evaluated the nutritional composition of ingredients and food samples typically consumed by the West African community in the United States. These samples were sourced from local African and Caribbean stores as well as various African households. The samples were dried, milled, and stored below 5°C in preparation for analysis. Samples were analyzed for protein, moisture, ash, fat, and starch content. Nutrient analysis shows that melon seeds (egusi commonly used for soup) contains 31.43% protein, 43.96% total fat, 5.95% total starch, njangsa seed contains 23.31% protein, 32.7% total fat, 16.58% total starch, bush mango contains 8.37% protein, 52.38% total fat, 23.58% total starch, cray fish contains 63.01% protein, 21.2% total fat, 0% total starch, African snail contains 33.16% protein, 8.67% total fat, 2.89% total starch, skinless peanut contains 28.57% protein, 34.22% total fat, 5.89% total starch, ripe plantain contains 3.38% protein, 0% total fat, 52.9% total starch, unripe plantain contains 3.62% protein, 0% total fat, 68.6% total starch, African yam contains 4.86% protein, 0% total fat, 59.97% total starch, white-garri contains 1.3% protein, 0% total fat, 56% total starch, semolina contains 15.59% protein, 2.28% total fat, 78.76% total starch, yam-fufu contains 5.78% protein, 0% total fat, 68.94% total starch, koki beans (black eye peas) contains 8.93% protein, 2.48% total fat, 38.38% total starch, and koki-corn contains 9.23% protein, 27.89% total fat, 27.53% total starch. We also evaluated the nutrient composition of chin-chin (snack) which is widely consumed in the West African Community. Nutrient analysis of fried chin-chin contains 1.0% moisture, 8.0% protein, 30.4% fat, 59.6% carbohydrate and a cup of chin-chin yielded 544 calories. Whereas, nutrient analysis of baked chin-chin contains 2.7% moisture, 10.3% protein, 11.2% fat, 74.5% carbohydrate and a cup of chin-chin yielded 440 calories. The fatty acid profile of palm oil that is widely used for cooking meals in the West African community was also evaluated. Analysis showed that palm oil imported from Cameroon contains48.77% saturated fat, 42.62% monounsaturated fat and 11.93% polyunsaturated fat. Furthermore, we also evaluated the nutritional composition of ingredients and food samples typically consumed by the East African community in the United States. We collected a total of 16 cooked food samples from households in Dover, Delaware, and Mill Creek, Washington, between August and November 2024. Samples were freeze-dried, pulverized, and analyzed for their proximate composition, total dietary fiber, starch content, mineral, amino acid and fatty acid composition using standard AOAC and AACC methods, gas chromatography, and high-performance liquid chromatography. The results showed wide variability across food samples with protein content ranging from 5.16% in Matoke (MAT) to 42.00% in Igisafuriya (IGY); lipid content ranged from 0.10% in Lumonde (LUD) to 50.11% in Sukuma Wiki (SKW); and fiber was highest in Imvange (IMG) at 16.42%. Starch content peaked in MAT (29.14%) and was lowest in Ebinyebwe (EBB) (1.72%). Isombe (ISB) exhibited elevated mineral content, including Ca (2180.00 µg/g), Na (20,160.00 µg/g), and Mn (43.57 µg/g), while Agatogo (AGG) had the highest Fe (69.96 µg/g), and Zn (78.07 µg/g) contents. Amino acid analysis indicated that IMG and AGG had the most favorable profiles, with high levels of valine (9.77%), methionine (8.52%), and lysine (2.73%), whereas Matooke (MTK) and ISB showed negligible essential amino acid content. Among fatty acids, polyunsaturated fatty acids (PUFA) were highest in MTK (14.25%), monounsaturated fatty acids (MUFA) in EBB (13.65%), and saturated fatty acids (SFA) in SKW (91.15%). These findings provide a scientific basis for developing a culturally relevant food composition table to guide dietary recommendations for African-born populations in the U.S.

Publications


    Progress 05/15/23 to 05/14/24

    Outputs
    Target Audience:African born immigrant population in the USA Undergraduate and Graduate students Cooperative Extension Specialists Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?A research associate and an undergraduate student participated in administration of the survey, collection of survey data and analysis of survey data in Microsoft Excel. The Research Associate, an undergraduate student and a graduate student have collected food samples that are commonly consumed by African-born immigrants, developed protocolsand are performing proximate analysis of the food samples. How have the results been disseminated to communities of interest?A survey was conducted to assess the eating habits of African-born immigrants. The results of the nutrition survey were presented during the 2024 Association of 1890 Research Directors symposium held in Nashville, TN, April 6-9, 2024. Survey results were disseminated to the target population through focus group meetings and WhatsApp. What do you plan to do during the next reporting period to accomplish the goals?Project directors and research associates will continue to conduct nutrient analysis of the cultural staple foods and determine the recommended portion size for selected immigrant staple foods. Data from food analysis will be used to develop Food Pattern Equivalents that will assist healthcare providers in delivering evidence-based educational programs to African clientele and enable them to make better food choices. PDs will determine Food Pattern Equivalents (Serving Sizes) for African Cultural Foods. PDs will collaborate dietetics students and EFNEP educators to develop culturally tailored intervention programs based on nutrition assessment survey results. PDs will continue to train undergraduate and graduate students in the food chemistry laboratory.

    Impacts
    What was accomplished under these goals? African-born immigrants (ABI) in the United States are the least-studied immigrant group. Obesity, hypertension, cardiovascular diseases, and type II diabetes are significant public health challenges, affecting not only Americans but many immigrant populations. Lack of knowledge of proper food portion sizes has been reported as one of the barriers in the management of obesity and diabetes among African-born immigrants. Epidemiological data revealed that rising levels of obesity and diabetes among African-born immigrants contribute to rising healthcare costs. Diet is a significant, modifiable risk factor associated with obesity and type II diabetes, and plays a crucial role in weight control and management of diabetes, hypertension, and cardiovascular diseases. Program Directors organized a meeting after the Award letter was received to review project activities and project timeline. Project directors (PD) developed a job description and announcement for a post-doctoral research associate position. The PDs screened, interviewed, and hired a candidate for the post-doctoral position. The PDs have developed a thirty-question questionnaire to assess the perceptions of healthy eating habits among African born immigrants. The questionnaire will be pre-tested in the Introduction to Nutrition Class prior to administration to target population. A letter of Consent, Protocol and IRB application has been completed for submission to the DSU's Institutional Review Board (IRB) for approval prior to conducting the survey. The project aims to: 1) assess dietary habits among African-born immigrants (ABI) in the United States, 2) develop and promote culturally tailored education programs to support healthy eating behaviors, and 3) develop a train-the-trainer program through formal and informal education programs. To assess dietary habits among ABI, a thirty-two-nutrition knowledge questionnaire tested and validated was used to measure awareness and nutrition knowledge. The questionnaire was structured to gather information on: (1) socio-demographics, (2) food preference, and (3) consumption behavior. The questionnaire was administered to 300 participants within the target population electronically via SurveyMonkey. A total of 200 participants completed the survey. Data collected and analyzed in Microsoft Excel, and results were expressed in percentages. Males (53%) constituted most of the study participants surveyed, and most of the participants (56%) were older than 51 years and have completed BS degree programs. About 79% of these participants have a full-time employment. The study revealed that most (70%) participants have lived in the US more than 10 years. Twenty percent of participants were diagnosed with diabetes, and 68% of this group reported that they have Type 2 diabetes, 24% have type 1 diabetes and 8% have gestational diabetes. Majority (91%) of the participants are not aware and have never participated in a diabetes education program. Forty-four percent (44%) of participants reported that they were diagnosed with high blood pressure and 64% reported that they gained body weight since immigrated to the US. Based on our assessment of eating behavior, study participants consume mostly (47%) food from country of birth and some American food and eat more (73%) home prepared meals. Some (42%) participants could not identify low glycemic index foods from foods they frequently consume. Most (60%) participants strongly agree that if they know the composition of their staple foods it would help them make healthful food choices. About 84% of participants reported that olive oil a healthier cooking oil than other commercial oils. We found that the study participants consume more (59%) of vitamin D and more (39%) calcium. Results from this study will assist in the development of culturally and contextually appropriate approaches to tackle food and nutrition insecurity and prevent and control diet-associated chronic diseases and health disparities in this community. To estimate the nutrient composition and create an extensive nutrient composition database for staple African foods prevalent among African-born immigrants, we evaluated the nutritional composition of ingredients and food samples typically consumed by the West African community in the United States. These samples were sourced from local African and Caribbean stores as well as various African households. The samples were dried, and milled, and stored below 5°C in preparation for analysis. Samples were analyzed for protein, moisture, ash, fat, and starch content. Nutrient analysis shows that melon seeds (egusi commonly used for soup) contains 31.43% protein, 43.96% total fat, 5.95% total starch, njangsa seed contains 23.31% protein, 32.7% total fat, 16.58% total starch, bush mango contains 8.37% protein, 52.38% total fat, 23.58% total starch, cray fish contains 63.01% protein, 21.2% total fat, 0% total starch, African snail contains 33.16% protein, 8.67% total fat, 2.89% total starch, skinless peanut contains 28.57% protein, 34.22% total fat, 5.89% total starch, ripe plantain contains 3.38% protein, 0% total fat, 52.9% total starch, unripe plantain contains 3.62% protein, 0% total fat, 68.6% total starch, African yam contains 4.86% protein, 0% total fat, 59.97% total starch, white-garri contains 1.3% protein, 0% total fat, 56% total starch, semolina contains 15.59% protein, 2.28% total fat, 78.76% total starch, yam-fufu contains 5.78% protein, 0% total fat, 68.94% total starch, koki beans (black eye peas) contains 8.93% protein, 2.48% total fat, 38.38% total starch, and koki-corn contains 9.23% protein, 27.89% total fat, 27.53% total starch.

    Publications


      Progress 05/15/22 to 05/14/23

      Outputs
      Target Audience:African-born immigrants inthe United States Undergraduate and Graduate students Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?The Project Directorsplan to conduct the survey to assess African-born immigrant community perceptions of healthy eating habits, conduct diet analysis of the identified staple foods and determine the recommended portion size for the selected immigrant staple foods and develop Training/Educational materials in collaboration with the community-community engagement. PDs plan to summarize project findings for scientific presentation and manuscript(s).

      Impacts
      What was accomplished under these goals? Program Directors organized a meeting after the Award letter was received to review project activities and project timeline. Project directors (PD) developed a job description and announcement for a post-doctoral research associate position. The PDs screened, interviewed, and hired a candidate for the post-doctoral position. The PDs have developed a thirty-question questionnaire to assess the perceptions of healthy eating habits among African born immigrants. The questionnaire will be pre-tested in the Introduction to Nutrition Class prior to administration to target population. A letter of Consent, Protocol and IRB application has been completed for submission to the DSU's Institutional Review Board (IRB) for approval prior to conducting the survey.

      Publications