Source: PENNSYLVANIA STATE UNIVERSITY submitted to NRP
UNDERSTANDING THE EFFECT OF DIETS FED TO DAIRY COWS ON THE MELTING PROPERTIES OF MILK FAT
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1028361
Grant No.
2022-68008-37106
Cumulative Award Amt.
$299,940.00
Proposal No.
2021-09858
Multistate No.
(N/A)
Project Start Date
Mar 1, 2022
Project End Date
Feb 28, 2026
Grant Year
2022
Program Code
[A1701]- Critical Agricultural Research and Extension: CARE
Recipient Organization
PENNSYLVANIA STATE UNIVERSITY
408 Old Main
UNIVERSITY PARK,PA 16802-1505
Performing Department
Animal Science
Non Technical Summary
The complex melting characteristics of butter are important to its versatility and key to physical properties of butter and other high fat dairy products. Milk fatty acid profile has an impact on the spreadability of butter and recent controversies have proposed that diets fed to dairy cows may be impacting butter properties in a negative way. We hypothesize that some diets that are commonly fed to dairy cows that change milk fatty acid profile impact the melting characteristics of milk fat. We will use milk fat from previous experiments that caused changes in milk fatty acid profile and validate these responses in butter. Our unique collaborative team will also deliver the findings through a diverse set of Extension activities. Our overall goal is to improve the quality of butter and milk fat products by better understanding the connections between cow management and finished products and educate dairy producers and nutritionists, milk processors, and consumers on key factors important to producing high quality dairy products.We expect this project will vastly expand our understanding of the relationship between diets, milk fatty acid profile, and melting properties of milk fat and will deliver this information to both producers and processors. This will have direct application to solve a critical and emerging issue for producers, processors, and consumers. We expect the ability to produce butter and other dairy products with ideal properties will increase demand for US dairy products by reducing use of vegetable oil spreads and imported products with more ideal properties.
Animal Health Component
100%
Research Effort Categories
Basic
(N/A)
Applied
100%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3083450101020%
3053420101020%
3083420101060%
Goals / Objectives
Our overall goal is to improve the quality of butter and milk fat products by better understanding the connections between diets fed to cows and finished products and educate dairy producers and nutritionists, milk processors, and consumers on key factors important to producing products that better meets consumer demands.Specific Aims1. Research Aim: Characterize the effect of diets fed to dairy cows on melting properties of milk fat.2. Extension Aim: Extension programming for dairy nutritionists, producers and processors.
Project Methods
RESEARCH AIM: Characterize the effect of diets fed to dairy cows on melting properties of milk fat.We will first characterization of melting properties of milk fat from previously conducted experiments that modified milk fatty acid profile. The experiments will include experiments that 1. fed a milk fat depressing diet that increased trans 18:1 in milk fat, 2. Fed increasing levels of oilseeds, and 3. Fed supplements that contained differing levels of palmitic and stearic acid. Physical properties of extracted milk fat will be characterized using multiple methods. First, melting and solidification behavior of anhydrous milk fat samples will be measured by differential scanning calorimeter (DSC). Second, solid fat content with be measured by nuclear magnetic resonance spectrometer. Lastly, crystalline polymorphisms of selected samples will be evaluated by X-ray diffraction.Next, we will verify the effect of palmitic and oleic acid on melting properties of butter. Eight ruminally cannulated cows will be arranged in a 4x4 Latin square design. Treatments will be a no fat control and abomasal infusion of an emulsion of highly enriched palmitic acid, oleic acid, or stearic acid. Milk will also be collected on the last day of each period for preparation of butter. Physical properties of extracted milk fat and butter will be characterized by DSC and NMR as described above.EXTENSION AIM: Extension programming for dairy nutritionists and producers and dairy processors.Background information and research findings will be communicated with dairy producers, dairy nutritionists, and other dairy industry professionals through a variety of face-to-face (regional conferences), on-line programing (webinars and videos), and written articles (on-line newsletters and Extension Bulletins). To maximize the reach from the current project, the proposed materials and programs will be incorporated into existing and well-recognized programs from Penn State Extension. Extension programming to be developed as part of the current project through 1. webinars focused, 2. Workshops and Conferences, 3. Videos, 4. On-Line Newsletter, 5. Print Newsletter, and 6. Lectures at workshops and conferences. Topics covered will focus on milk fat quality factors are important to processors and consumers, how feeding strategies on finished dairy product quality and functional properties, and how to manage dairy cow nutrition to modify the functional properties of dairy foods and ingredients.

Progress 03/01/23 to 02/29/24

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The project continued to be the main focus of one PhD student. An additional graduate students was training in the analysis techniques and applied the procedures to characterize melting properties in milk fat from a study feeding increasing levels of palmitic and stearic acid. There were also multiple undergraduate students involved in assisting in sample collection and processing. How have the results been disseminated to communities of interest?There was a presentation on effect of feeding strategies on melting properties of milk fat presented at the PSU Dairy Nutrition Workshop. What do you plan to do during the next reporting period to accomplish the goals?We will prepare 2 manuscripts from the data collected on increasing oilseeds and effect of palmitic and palmitic acid blends on melting properties. Additionally, we will characterize the effect of increasing oleic acid and other polyunsaturated fatty acids on melting properties. Lastly, we will prepare extension bulletins.

Impacts
What was accomplished under these goals? During the reporting period we analyzed melting properties of 4 experiments using differential scanning calorimetry and two new experiments using NMR to quantify percent solid milk fat in two new experiments.

Publications

  • Type: Conference Papers and Presentations Status: Other Year Published: 2023 Citation: K.J. Harvatine, S. Burtnett, and A. Staffin. 2023. Update on fat supplements and how dietary fat impacts melting properties of milk fat. Penn State Dairy Nutrition Workshop, Hershey, PA. Nov 9th.
  • Type: Conference Papers and Presentations Status: Submitted Year Published: 2024 Citation: 13. Husnain, M., H. Reisinger, G. Ziegler, K. Kaylegian, and K. Harvatine. 2024. Effect of increasing dietary fat by feeding oilseeds on melting properties of milk fat in Holstein cows. Submitted to ADSA Annual Meeting
  • Type: Conference Papers and Presentations Status: Submitted Year Published: 2024 Citation: Husnain, M., G. Ziegler, K. Kaylegian, and K. Harvatine. 2024.The effect of palmitic acid fat supplements on the melting properties of milk fat of Holstein cows. Submitted to ADSA Annual Meeting


Progress 03/01/22 to 02/28/23

Outputs
Target Audience:Research and field nutritionists, dairy producers, and dairy processors. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?One PhD graduate student has been engaged in the project as the main part of his PhD dissertation. Undergraduate students also were involved with sample processing and analysis. How have the results been disseminated to communities of interest?A talk on the aspects of milk fat important to milk processing was presented at the Penn State Dairy Nutrition Workshop. What do you plan to do during the next reporting period to accomplish the goals?We will complete data analysis of the differential scanning calorimetry data collected, will conduct melting analysis of additional experiments, and initiate the animal experiment that will investigate the effect of increasing oleic acid. We will also submit manuscripts from the work and develop extension materials.

Impacts
What was accomplished under these goals? We have used differential scanning calorimetry to characterize melting properties of milk fat from threepreviously conducted experiments that investigated feeding increasing levels of cottonseed and roasted soybeans and fat supplements that differ in palmitic and stearic acid. We also characterized melting properties of milk fat from a recently conducted trial investing palmitic acid and diet-induced milk fat depression.

Publications

  • Type: Conference Papers and Presentations Status: Other Year Published: 2022 Citation: Kaylegian, K. 2022. Properties of milk fat important to dairy products. Penn State Dairy Cattle Nutrition Workshop. Hershey, PA. Nov 3rd.