Source: TARLETON STATE UNIVERSITY - TIAER submitted to NRP
COMBING REPLICATED LARGE-SCALE AQUAPONIC MEDIA BED RESEARCH AND PRODUCER BASED EXTENSION TO ADDRESS CRITICAL ISSUES OF AQUAPONIC PRODUCERS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1028258
Grant No.
2022-68008-37107
Cumulative Award Amt.
$298,262.00
Proposal No.
2021-09872
Multistate No.
(N/A)
Project Start Date
Apr 15, 2022
Project End Date
Apr 14, 2026
Grant Year
2022
Program Code
[A1701]- Critical Agricultural Research and Extension: CARE
Recipient Organization
TARLETON STATE UNIVERSITY - TIAER
201 Saint Felix Street
STEPHENVILLE,TX 76402
Performing Department
Wildlife Sustainability & Ecos
Non Technical Summary
Agricultural food production must increase with human population, but production will have to do so with less arable land and less freshwater. This is a critical issue as a majority of our food is produced using soil-based agriculture techniques in breadbasket locations which require large amounts of both arable land and freshwater. Additionally, this decrease in arable land and freshwater is only expected to be further exacerbated by the effects of climate change and increased growth across the urban-rural continuum. While soil-based agriculture production is a major catalyst of climate change, it is also a major victim of the effects of climate change. The USDA estimated that from 1980-2010 over $700 billion worth of crop losses were caused by over 90 extreme events (droughts, floods, tornadoes, etc.) that each resulted in more than $1 billion worth of damages. In 2020, there were a record setting 22 $1 billion disasters caused by extreme weather events. This number will undoubtedly continue to increase as a result of today's climate conditions, which will continue to result in huge crop and economic losses. It is not ecologically nor economically sustainable to continue relying heavily on soil-based agriculture. Therefore, we must adopt alternative agriculture systems that are closer to markets and use less-resources (fresh water, arable land, and petroleum-based chemicals) to produce higher yields in order to achieve food security for a growing population in an ever-changing climate.One alternative agriculture production system with increasing global recognition as a solution to some of the near future issues agriculture production faces is aquaponics. Aquaponics integrates recirculating aquaculture systems (RAS) and soilless plant production (hydroponics) into a single closed/semi-closed loop system. Aquaponics does not require arable land and produces more per square foot with 90% less water than traditional soil-based production and without using synthetic fertilizers or pesticides. Additionally, aquaponics can be integrated virtually anywhere with access to freshwater and electricity. The integration of two advanced production technologies (RAS and hydroponics) has created an equally efficient yet less environmentally impactful production system when compared to RAS, hydroponics, or soil-based agriculture alone. However, aquaponics is still considered a new and emerging alternative agriculture system that lacks skilled personnel and has technical challenges associated with the technology that are considered critical and must be addressed in order to continue facilitating this market growth. Some of these critical challenges are nutrient availability, infrastructure and component costs, system design improvements, annual environmental control costs (e.g., heating the water during cold weather), financing, and waste management. Replicated large-scale aquaponic research systems incorporated into institutions of higher learning have the ability to provide students and researchers with a valuable multidisciplinary tool to address critical issues faced by aquaponic producers. However, the dissemination of the information through established extension and industry networks is required to help build a stronger local and regional industry network. Building a stronger aquaponic network and industry will be crucial to allow for the continual growth of the industry. The growth of the aquaponics industry will benefit the agriculture sector by building a healthier, more resilient, localized, and productive industry, which benefits the local, national, and global economy.This project intends to blend research from replicated large-scale media bed aquaponic systems with education and extension in an effort to address critical needs that hinder the growth of the aquaponic industry. Dr. Hennen Cummings of TSU will serve as the PI for this grant. Research would be conducted at The Hydrotron, an aquaponic demonstration greenhouse and learning center at TSU. Symbiotic Aquaponic LLC would partner with the university in effort to bridge the gap between industry and institution-led research. Symbiotic Aquaponic will help build the replicated systems, connect local producers, contribute to curriculum building and workshop activities with Dr. Cummings, his students, and the community. The overarching goal of this project is to provide novel aquaponic research and critical extension support for the industry that continues beyond the duration of this project. This will be done by training skilled personnel and conducting research that provides producers with biological solutions to improve production and efficiency of their media bed systems, improve profitability of aquaponic production, addresses food security, and establishes an aquaponic extension outreach center at Tarleton State University. In order to achieve the projects goal, multiple objectives will be undertaken. These include: 1) Four research projects, 2) Two-part free virtual workshop series, 3) Enhanced aquaponics curriculum, 4) Three-part factsheet, 5) One Texas statewide survey, 6) 2+ Peer-reviewed Journal publications, and 7) 6+ experienced and skilled students involved in aquaponics. Overall this project intends to strengthen the aquaponics industry by generating meaningful data that can be applied to commercial aquaponic production, providing technical assistance to any producer through the extension outreach center, and spark aquaponic collaborations within and outside the state of Texas. By strengthening the aquaponics industry, we are diversifying the agricultural industry, while strengthening food security, and producing food in a manner that is significantly less harmful to the environment.
Animal Health Component
50%
Research Effort Categories
Basic
20%
Applied
50%
Developmental
30%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20514301060100%
Goals / Objectives
The goal of this project is to provide novel agriculture research and critical extension support for the aquaponics industry by training skilled personnel and conducting research that provides producers with biological solutions to improve production and efficiency of their media bed systems and to establish an extension outreach center at Tarleton State University. In order to achieve this goal, three main objectives encompass this project: A) Critical Research, B) Critical Extension, and C) Continuation Plan.Critical Research The research will seek to increase production through biological solutions within media bed aquaponic systems. These biological solutions intend to provide a cost-effective way of diversifying and improving system efficiency in terms of nutrient availability, plant nutrient uptake, and waste management. Both undergraduate and graduate students will be employed to conduct novel research that provides needed data for the aquaponic industry and also provides solutions for increasing media bed aquaponic production. By relying on students this project will also provide training and create skilled individuals that are equipped to operate within the aquaponics industry. Four separate studies will be undertaken as part of the research objective these studies are listed below. The studies with an "**" at the end of the title are intended to be graduate student thesis projects while the other two projects are intended to be undergraduate research projects.- Evaluate the use of Mexican scuds, Hyalella Azteca, in media bed aquaponic systems and compare systems receiving scuds with systems not receiving scuds. **- Evaluate the use of aerobic biodigestion (mineralization) for orthophosphate recovery from fish effluent removed from aquaponic systems receiving scuds and no scuds overtime.- Evaluate the use of black solider fly larvae (BSFL) as a decomposer of unusable fish and plant parts generated in aquaponics.- Evaluate the use of Plant Growth Promoting Microorganisms (PGPM) in aquaponic media bed systems. **Critical Extension will consist of creating an outreach center at Tarleton State University (TSU) that will distribute data and results produced from the critical research to stakeholders, practitioners, educators, community members, and fellow researchers. Throughout the duration of this project, the outreach center will also provide two free workshops, three factsheets, creation of Aquaponics 2 Course, and a survey of Texas aquaponic practitioners. These extension deliverables will be created and delivered through the TSU and Symbiotic partnership. The two workshops and factsheets will be used to serve TSU stakeholders and to provide a free learning opportunity for interested individuals regionally and nationally. The survey will be used to assess the scale of aquaponic production within Texas as well as the issues that producers face. Additionally, critical extension will consist of building an aquaponics curriculum at TSU to attract more prospective students who are interested in learning more about aquaponics. Attracting prospective students to Tarleton is important, as it is an associate member of the Hispanic Association of Colleges and Universities, and it has been recognized as an emerging Hispanic serving institution with 21% of their students coming from a Hispanic or Latino ethnic background. Involving minority students in an up-and-coming industry is critical to ensuring a diverse group of skilled personnel is available in this job market.The two workshops are listed below:Workshop 1: Basic and Advanced productionWorkshop 2: Community/STEM ImplicationsThe three factsheets are listed below:Factsheet 1: System Design and OperationFactsheet 2: Waste ManagementFactsheet 3: Improving Profitability of AquaponicsContinuation Plan will be implemented to ensure that both the Critical Research and Extension will continue beyond the duration of this project. This continuation plan will utilize the survey to provide focused research projects that address the issues that aquaponic practitioners face in the state of Texas. Additionally, the continuation plan would ensure a continuous stream of skilled laborers and future researchers beyond the duration of this project by offering advanced aquaponic curriculum, workforce development trainings, and research opportunities at TSU. Many high school groups, potential Tarleton students, boy scouts, girl scouts, FFA, 4H, Leadership Stephenville, Master Gardeners, Master Naturalists, and entrepreneurs will continue to tour the facility.
Project Methods
A) Efforts:- Development of aquaponic curriculum and laboratory instruction for the Aquaponic (AQP) 2 Course that will be offered at TSU. Hennen Cummings and other TSU staff will direct this effort. This course would be offered in the 2022 fall semester following the spring AQP 1 class and would require the completion of the AQP 1 course. This course will build off the AQP 1 course and will offer practical learning outcomes that focus on three main categories: 1) water quality and plant health, 2) fish production and reproduction, and 3) greenhouse management. The water quality and plant health content will focus on the instruments and meters used to determine water quality and plant health. Learning will be aided through hands-on activity coupled by informational tasks that cover why these parameters are important for production and research. For the fish production and reproduction, students will learn fish reproduction, so they can create a self-sustaining fish population and prevent fish disease. The fish breeding system already present at TSU will be a hands-on tool that will allow students to work hands-on with fish reproduction. Lastly, students will learn greenhouse management that includes proper IPM, environmental control, and things to consider when utilizing a greenhouse for production. Overall, the AQP 2 class will enhance the learning opportunities offered at TSU regarding AQP and will help prepare students to become knowledgeable, skilled, and experienced workers for the aquaponics industry.- Development of aquaponic workshop material for the free two-part aquaponic workshop series. The workshops along with a brief background are listed below:Workshop 1: Basic and Advanced productionThis workshop will cover a variety of topics that include system construction, design, operation, and production. This workshop will be delivered by Dr. Hennen Cummings of TSU and Kaben Smallwood/Josh Dusci of Symbiotic Aquaponic.Workshop 2: Community/STEM ImplicationsThis workshop will cover food sovereignty, food safety, and food security. TSU will incorporate this workshop with its Youth Camp to provide a hands-on learning experience. Kaben Smallwood and Regina Greuel-Cook from Symbiotic Aquaponic will assist Dr. Hennen Cummings with delivering this content.- Development of aquaponic factsheet information for the free three-part aquaponic factsheet series. The factsheets along with a brief background are listed below:Factsheet 1: System Design and OperationThis factsheet will focus on common and uncommon system designs and the advantages and disadvantages of each design. This factsheet will also focus on calculating feed inputs, planting densities, Integrated Pest Management (IPM), and pre- /post-harvest considerations.Factsheet 2: Waste ManagementThis factsheet will focus on waste management within aquaponics and will incorporate the results from Research projects 1-3. The factsheet will be updated as results from the projects become available.Factsheet 3: Improving Profitability of AquaponicsThis factsheet will focus on the economics behind aquaponics and what can help increase profits in certain locations. The results from research projects 1-4 will be incorporated and the factsheet will be updated as results from the projects become available.- Generating aquaponic media bed research data through the four separate research projects. These projects are designed to produce data that can be published in a peer-reviewed scientific journal (Frontiers of Sustainable Agriculture). Research will be conducted on six replicated media bed systems (250 gal/system). These replicated systems will allow for proper statistical analysis to be conducted using ANOVA's and Least Significant Differences (LSD) tests. Once the data has been collected and analyzed, determining how to apply research outcomes to be useful for aquaponic practitioners will also be a key part of this project. Overall the research intends to lead to biological solutions to critical problems for aquaponic production, which include increasing system efficiency, increasing plant nutrient uptake, waste management, and improving the profitability of aquaponic production. Along with providing biological solutions to critical issues, this research will also allow for the training of skilled personnel (undergraduate and graduate students) to be prepared for aquaponic industry, which lacks skilled personnel.- Generating data from statewide survey to utilize for continuation plan. This statewide survey will be conducted by Tarleton using the assistance of Symbiotic Aquaponic and their industry connections within Texas, which include Texas State University, Texas A&M (Kingsville), Sam Houston University, and the 15+ clients that Symbiotic has delivered various sized systems to. Additionally, support from The Aquaponics Association will be relied on to provide a list of affiliates located within Texas. TSU will also actively advertise this survey to the public and to their stakeholders, which include Sustainable Harvesters (Hockley, TX), Breeden Fresh Farms (Terrell, TX), and East Texas Aquaponics (Mineola, TX). The educators include Stephenville High School, Independent School District, various FFA and 4H groups, Cesar Chaves Public Charter Schools, and Farming For The Future (Texas A&M) Program. This will allow for the statewide survey to reach as many aquaponic practitioners within Texas as possible. This statewide survey intends to provide insight as to what critical issues are currently hindering aquaponic producers in Texas. This insight will then be used to create future research and extension projects to address these issues for producers.B) Evaluation:- 100% completion of research projects and two successful peer-reviewed publications- 75+ workshop attendees per workshop- 60+ participants with statewide survey- 20+ students involved in Aquaponic 2 course each year after the creation- 50+ tours given annually of systems and outreach center- 6+ skilled laborers enter aquaponic job market- 500+ views and 100+ downloads of factsheets within first year of publicationIn order to assess the evaluation process, Dr. Cummings and TSU will send the funding agency quarterly and annual reports regarding the status of these evaluation marks.All research data will be collected in a non-digital format then transferred to digital for analysis and access. Data will include fish and plant production parameters, water quality, and other conditions of AQP systems. Manual data will be collected from test systems daily/weekly, with a total amount of data not to exceed two pages per day. Data will then be transferred to Microsoft Excel, which will not require significant storage space. Data collection is not highly iterative. The largest amount of data will be the two thesis research projects (Project 1: Scuds and Project 4: PGPM).All other data regarding participation and downloads/views will be actively recorded by TSU and will be included on the quarterly and annual reports.Use of funds will be actively recorded by TSU and will be included on the quarterly and annual reports to show transparency throughout the funding period.

Progress 04/15/24 to 04/14/25

Outputs
Target Audience:9/14-17/2023 Namraj Jaishi, Emma Louise Smith, and Emmanuel Johnson attended the 12th Annual Aquaponics Association Conference in Albuquerque, NM. Namraj presented his research. Namraj Jaishi presented a PowerPoint with the title "Fish solid waste reduction and nutrient solubilization using Mexican scud." 5/132025 Aquaponics Youth Camp from 8:00 - 2:00 for 17 students. 6/12/2024 Ag Camp tour of the Aquaponics Center (Hydrotron) for 15 campers. 6/14/2024 Aquaponics Adult Workshop. 9/12-15/2024 Emma Louise Smith and Emmanuel Johnson presented their research at the 13th Annual Aquaponics Association Conference in Dallas, TX. Emmanuel Johnson presented a PowerPoint with the title " Increasing Plant Production while Decreasing the Amount of Nutrients Leaving with Fish Solid Waste by Using Mexican Scuds (Hyalella azteca) in Aquaponic Systems." Emma Louise Smith presented a PowerPoint with the title "Evaluating Black Soldier Fly Larvae (Hermentia illucens) as a Feed Alternative for Tilapia in Aquaponics." 10/22/2024 Evaluating Black Soldier Fly Larvae as a Feed Alternative for Mozambique Tilapia in Aquaponics. Emma Louise Smith, Hennen Cummings, PhD, Adam Mitchell, PhD, and Kimberly Wellmann, PhD. Preparation of Papers for International Posgraduate Researching Colloquium (September 2024). Techologico Nacional De Mexico Online Conference. 10/25/2024 Increasing plant product while decreasing the amount of nutrients leaving with fish solid waste by using Mexican scuds (Hyalella azteca) in aquaponic systems. Emmanuel Johnson, Hennen Cummings, Dr Jim Muir, and Dr Victoria Chraibi. Preparation of Papers for International Posgraduate Researching Colloquium (September 2024). Techologico Nacional De Mexico Online Conference. Hosted many tours of the Aquaponics Center (Hydrotron) to school groups, camps, prospective students and their parents, civic clubs, Master Gardeners, Tarleton Vice Presidents, etc. Presentations on aquaponics to Master gardeners in Denton and Albiene, TX Changes/Problems:As stated in last year's report "Evaluating Mexican scuds in the media beds is challenging. Recovering and counting the Mexican scud to see if theirpopulation is increasing or decreasing is too challenging in a gravel root zone. Recovering and measuring lettuce root loss due to Mexican scuds is too difficult in media beds because of roots sticking to the media which introduces error into the measurements. Thus, lettuce is grown hydroponically in 37L tubs with 4 plants per tub. The lettuce is germinated in rock wool and transfered to the tubs. Each tub has an areation stone and heater. Half the tubs have scuds and the other half does not. A known number of scuds are added for 4 weeks and then counted at the end of the exeriment. This experiment is a worst case scenario where the only thing the scuds can eat is the lettuce roots and each other. The hydroponics nutrient solution is half strength due the the inability of scuds to survive in full strenght nutrient solution, and the lettuce grow well in half strength nutrient solution. Similarly, media beds confound recovery of fish feces and Mexican scuds. In order to determine if Mexican scuds can ingest fish solid waste and excreet soluble plant available nutrients. Fish feces is placed in 7 gal containers with an air stone and heater. Half of the containers receive a know number of equal size scud. At the end of the experiment, fish solid waste mass and volume is deterimined. The fish solid waste and water from each container is sent to A&M laboratory for analysis. The scud population in each container is counted. What opportunities for training and professional development has the project provided?Aquaponics Youth Camps Aquaponics Adult Workshop Aquaponics Assoication Conference How have the results been disseminated to communities of interest?Aquaponics Youth Camps Aquaponics Adult Workshop Aquaponics Assoication Conference Tours of the Aquaponics Center (Hydrotron) PowerPoint presentations to Master Gardeners Facebook. https://www.facebook.com/aquaponics.hydrotron What do you plan to do during the next reporting period to accomplish the goals?Emmanuel Johnson plans to submit his research for publication. Emma Louise Smith plans to submit her research for publication. Aquaponics Camp is planned for 5/30/2025 Aquaponics Adult Workshop is planned for 5/31/2025 PowerPoint presenations will be given to Denton and Abienen, TX Master Gardeners Dozens and dozens of tours of the Aquaponics Center (Hydrotron) will be conducted.

Impacts
What was accomplished under these goals? 9/14-17/2023 Namraj Jaishi, Emma Louise Smith, and Emmanuel Johnson attended the 12th Annual Aquaponics Association Conference in Albuquerque, NM. Namraj presented his research. Namraj Jaishi presented a PowerPoint with the title "Fish solid waste reduction and nutrient solubilization using Mexican scud." 5/132025 Aquaponics Youth Camp from 8:00 - 2:00 for 17 students. 6/12/2024 Ag Camp tour of the Aquaponics Center (Hydrotron) for 15 campers. 6/14/2024 Aquaponics Adult Workshop. 9/12-15/2024 Emma Louise Smith and Emmanuel Johnson presented their research at the 13th Annual Aquaponics Association Conference in Dallas, TX. Emmanuel Johnson presented a PowerPoint with the title " Increasing Plant Production while Decreasing the Amount of Nutrients Leaving with Fish Solid Waste by Using Mexican Scuds (Hyalella azteca) in Aquaponic Systems." Emma Louise Smith presented a PowerPoint with the title "Evaluating Black Soldier Fly Larvae (Hermentia illucens) as a Feed Alternative for Tilapia in Aquaponics." 10/22/2024 Evaluating Black Soldier Fly Larvae as a Feed Alternative for Mozambique Tilapia in Aquaponics. Emma Louise Smith, Hennen Cummings, PhD, Adam Mitchell, PhD, and Kimberly Wellmann, PhD. Preparation of Papers for International Posgraduate Researching Colloquium (September 2024). Techologico Nacional De Mexico Online Conference. 10/25/2024 Increasing plant product while decreasing the amount of nutrients leaving with fish solid waste by using Mexican scuds (Hyalella azteca) in aquaponic systems. Emmanuel Johnson, Hennen Cummings, Dr Jim Muir, and Dr Victoria Chraibi. Preparation of Papers for International Posgraduate Researching Colloquium (September 2024). Techologico Nacional De Mexico Online Conference. Emma Louise Smith built a lavarium which successfully grows black soldier fly lavare year round for her research tilapia feed trials.

Publications


    Progress 04/15/23 to 04/14/24

    Outputs
    Target Audience:Conducted Aquaponics Youth Camp on Fri. Jun. 2 for 15 camplers and Aquaponics Adult Workshop for 5 adults on Sat. June. 3, 2023. Namraj Jaishi presented a PowerPoint with the title "Fish solid waste reduction and nutrient solubilization using Mexican scud" at the the 12th Annual Aquaponics Association Conference in Albuquerque, NM on Sep. 15, 2023. Abstract In recent years, the use of Mexican scud (Hyalella azteca) in Aquaponics systems has gained attention as a promising practice. Scuds are tiny amphipods whose feeding behavior primarily involves consuming solid waste, plant debris, and algae. We started an experiment having three treatments in well aerated 26 L trash can ( 1.20 L water +100 scud + 300 ml fish waste 2. 20 L water + 300 ml fish waste 3. 20 L water + 100 scud) each with three replications. The results suggests that scuds are able to consume fish waste and reduce fish waste from 300 ml to 233 ml from the start to the end of the experiment. In addition to this, they are capable of increasing nutrient solubilization ( Nitrate from 2.4 to 9.7 mg/L, phosphate ) which are really beneficial to the aquaponics system. Changes/Problems:Evaluating Mexican scuds in the media beds is challenging. Recovering and counting the Mexican scud to see if their population is increasing or decreasing is too challenging in a gravel root zone. Recovering and measuring lettuce root loss due to Mexican scuds is too difficult in media beds because of roots sticking to the media which introduces error into the measurements. Thus, lettuce is grown hydroponically in 37L tubs with 4 plants per tub. The lettuce is germinated in rock wool and transfered to the tubs. Each tub has an areation stone and heater. Half the tubs have scuds and the other half does not. A known number of scuds are added for 4 weeks and then counted at the end of the exeriment. This experiment is a worst case scenario where the only thing the scuds can eat is the lettuce roots and each other. The hydroponics nutrient solution is half strength due the the inability of scuds to survive in full strenght nutrient solution, and the lettuce grow well in half strength nutrient solution. Similarly, media beds confound recovery of fish feces and Mexican scuds. In order to determine if Mexican scuds can ingest fish solid waste and excreet soluble plant available nutrients. Fish feces is placed in 7 gal containers with an air stone and heater. Half of the containers receive a know number of equal size scud. At the end of the experiment, fish solid waste mass and volume is deterimined. The fish solid waste and water from each container is sent to A&M laboratory for analysis. The scud population in each container is counted. What opportunities for training and professional development has the project provided?Emmanuel Johnson, Namraj Jaishi, and Emma Louis Smith attended the 12th Annual Aquaponics Association Conference in Albuquerque, NM from 9/14-9/17/2023. They also attended the Aquaculture America trade show in San Antonio, TX on 2/19/2024. These graduate students saw /read other students' PowerPoints and posters. They saw that some students are doing similar research and learned ways to present their data. Emmanuel Johnson and Emma Louis Smith attended the Aquaculture of America trade Show in San Antonio, TX on 2/18-2/19/2024. These graduate students saw /read other students' posters. They saw that some students are doing similar research and learned ways to present their data. How have the results been disseminated to communities of interest?Namraj Jaishi presented a PowerPoint with the title "Fish solid waste reduction and nutrient solubilization using Mexican scud" at the the 12th Annual Aquaponics Association Conference in Albuquerque, NM. An Aquponics Youth Camp and an Aquaponics Workshop for adults occured on 6/02/2023 for 15 youths and on 6/03/2023 5 adults, respectively. What do you plan to do during the next reporting period to accomplish the goals?Emmanuel Johnson will continue investigate the ability of Mexican Scud to reduce fish solid waste and increase dissolved plant available nutrients. Mexican scud are considered scavengers; however, they may eat lettuce roots in a aquaponics system as they will become omnipresent in the system which is undersireable. Thus, lettuce will be grown in ten 34L containers with half strength hydroponics nutrient solution with and without Mexican scud and four lettuce plants germinated in rockwool. The experiment is a worst case scenario where the only thing for scud to eat are the roots and other Mexican scud. The nutrient solution is half strength because scud cannot survive the salt concentration in full strength hydroponic nutrient solution, but the plants develop as they would in full strength nutrient solution. Mexican scud population change, lettuce head height, width, mass, and quality, and root length and mass will be measured. Chlorphyl contant will also be measured. Emmanuel Johnson will evaluate the use of aerobic biodigestion (mineralization) for orthophosphate recovery from fish effluent removed from aquaponic systems receiving scuds and no scuds overtime using the same apparatus used to determine the ability of Mexican scud to reduce fish solid waste and increased the dissolved nutrients. A goal is to minimized the nutrients removed from the system as fish solid waste and increase the amount of nutrients removed from the system in the harvested lettuce. Emma Louise Smith will investigate juvenile tilaplia growth using conventional fish feed with and without black soldier fly larvae as a protein supliment as well as conventional feed with and without algae wafferes as a protein suplement. The larvae are fed tilapia fillet remnants and mortalities from the main aquaponics system at Tarleton State University. Emma Louise Smith will investigate the use of Plant Growth Promoting Microorganisms (PGPM) using the eight 37L tubs with 4 plants each. This research is conducted in tubs instead of media beds because quantifying roots in media beds is confounded by loss of roots due the the difficulty of removing the roots from the media in the media beds. In this case the lettuce is gown in aquaponics water instead of hydroponic nutrient solution. An Aquponics Youth Camp and an Aquaponics Workshop for adults in scheduled for 05/31/2024 and 06/01/2024, respectively.

    Impacts
    What was accomplished under these goals? The ability of Mexican scud to reduce the volume of solid fish waste and liberate more dissolved, plant available nutrients was evaluated in another run by Emmanuel Johnson, and the the samples were sent to the Texas A&M Soil, Water, and Forage Testing Laboratory on Feb. 28, 2024. Emmanuel is using 8 7gal tubs. All tubs have fish feces. Half the tubs have scud. Want to see if Mexican scud can reduce feces volume and mass and increase the concentration of disolved plant available nutrients. A larvarium was constructed making a screened in closet containing ProtaPod: Black Soldier Fly Larvae (BSFL) Food Waste Digester - 4 Foot Homestead Style Pod. The larvarium is in a greenhouse. The larvae will be fed to tilapia in the same greenhouse. There is 1 tilapia in each of the six 55-gal barrels. Each barrel has an airstone and heater. Samples of the larvae are being analyzed for protein content inorder to determine how to add the same amount of protein to all six barrels using larvae and conventional fish feed vs. conventional fish feed vs. algae as protein sources. A permit to have tilapia in the greenhouse was obtained from the Texas A&M Institutional Animal Care and Use Committee (IACUC). An Aquponics Youth Camp and an Aquaponics Workshop for adults occured on 6/02/2023 for 15 youths and on 6/03/2023 5 adults, respectively. Symbiotic aquaponics shared workshop information inorder to host an online virtual workshop when more data from this research is ready for publication.

    Publications


      Progress 04/15/22 to 04/14/23

      Outputs
      Target Audience: Nothing Reported Changes/Problems:The impact of Mexican scud on lettuce rooting is more difficult to determine in a media bed (gravel) system because the roots are more difficult to collect because they latch onto the gravel and can break off during harvest. The mass of scud and change in poplulations are difficult to quantify in the media bed system. The reduction is fish solid waste is difficult to measurein the media bed systems. Thus the Mexican scud are being evaluated in 17 gal tubs without media in order to set up a worst case scenario where the only food for the scud are the lettuce roots and each other. We can measure root loss and changes in scud populations more accurately and more precisely. In tubs we can measure the reduction of fish solid waste and impact on the concetration of plant available dissolved nutrients more accurately and precisely than in the media beds. Hiring a graduate student from Nepal took more time than expected to relocate to Stephenville TX. A prospective graduate student from Nigeria is having trouble obtaining a visa and may not be able to obtain one in time. In response an undergraduate student graduating in Spring 2023 was offered the position. My department head graciously offered an assistanceship to the prospective student from Nigeria if he is able to obtain therequired visa and funds to relocate to Stephenville, TX. What opportunities for training and professional development has the project provided?Tours of the aquponics facilitites occur every week for a wide variety of audiences of all ages. The aquaponics class and lab, HORT/WSES 3309, is learning about the autosiphon in the media bed. The class is learning about water quality parameters like ammonia, nitrite, nitrate, temperature, pH, alkalinity, total dissolved solids, and dissolved oxygen and how to make adjustments to address parameters that are outside of the optimum ranges. The class helped establish the tilapia in the 6 systems by making certain the the mass and number of fish amongst the systems is uniform. The graduate students are calculatingthe propper amount of fish feed and suppliments to add to the tanks. They are learning how to removeand clean the pump, inline heaters, and filters. The graduate students are learning how to control pests in the organic systems. How have the results been disseminated to communities of interest?Prelimary results are presented during tours and to the students in the aquaponics class and lab. What do you plan to do during the next reporting period to accomplish the goals?A) Critical Research, B) Critical Extension, and C) Continuation Plan. A) Critical Research 1) Evaluate the use of Mexican scuds, Hyalella Azteca, in media bed aquaponic systems and compare systems receiving scuds with systems not receiving scuds.** Data for the Mexican scud and their impact on lettuce in the replicated tubs will be compiled and summarized. 2) Evaluate the use of aerobic biodigestion (mineralization) for orthophosphate recovery from fish effluent removed from aquaponic systems receiving scuds and no scuds overtime. Samples will be analyzed by a lab to help increase the efficiency of orthophosphate reccovery. The composted fish feces barrels are installed and processing fish feces for use as plant suppliments. 3) Evaluate the use of black solider fly larvae (BSFL) as a decomposer of unusable fish and plant parts generated in aquaponics. The BSFL building used to house the BSFL rearing systems will be installed. The the goal of the building is to contain the adult black soldier fly and exclude other flies in order to set up a self pertuating colony of black soldier fly.The amount of fish wasted processed by the BSFL and the yield of BSFL will be recorded. Juvenile tilapis will be fed either the BSFL and fish feed or just fish feed and the impact of BSFL in diet will be evaluated by measuring fish body mass and bones. 4) Evaluate the use of Plant Growth Promoting Microorganisms (PGPM) in aquaponic media bed systems.** Nothing to report. B) Critical Extension Workshop 1: Basic and Advanced production Videos will be filmed to increase the capacity to engage the online audience. In person workshops for adults and camps for youths will be conducted in June 2023. Workshop 2: Community/STEM Implications Nothing to report The three factsheets are listed below: Factsheet 1: System Design and Operation Drafts fact sheets will be editted. Factsheet 2: Waste Management Drafts fact sheets will be editted based on the results of this grant. Factsheet 3: Improving Profitability of Aquaponics Drafts fact sheets will be editted. C) Continuation Plan Tours of the aquponics facilitites occur ever week for a wide variety of audiences of all ages.

      Impacts
      What was accomplished under these goals? A) Critical Research, B) Critical Extension, and C) Continuation Plan. A) Critical Research 1) Evaluate the use of Mexican scuds, Hyalella Azteca, in media bed aquaponic systems and compare systems receiving scuds with systems not receiving scuds.** Six repicated aquaponics systems have been installed and are functioning as they should (Microbes are converting ammonia to nitrate). The mass of fish is the same amongst all tanks. Several iterations of growing lettuce and controlling pest have been accomplished in order to correct any bias amonst the systems. Twograduate students are maintaining these systems. The graduate student from Napal, Namraj Jaishi is woking with 10replicated 17 gal tubs to determine the impact of Mexican scud on lettuce rooting where the number of scud can be measured as well as scud population changes since scud may self predate or reproduce. The complete root systems is easier to havest from a raft system than a media bed system. These systems create a worst case scenario where the only food source for the scud is the lettuce roots and each other. The ability of Mexican scud to reduce the mass of fish solid wasteand increase the plant available disolved nutrients contrations is also being determined. Mexican scud will be be added to half of the replicated systems in summer 2023. 2) Evaluate the use of aerobic biodigestion (mineralization) for orthophosphate recovery from fish effluent removed from aquaponic systems receiving scuds and no scuds overtime. The composted fish feces barrels are installed and processing fish feces for use as plant suppliments. The liquid sepparated from the solids contains plant avaiable nutrients and is returned to the aquaponics system. 3) Evaluate the use of black solider fly larvae (BSFL) as a decomposer of unusable fish and plant parts generated in aquaponics. A graduate student from Texas, Emma Louise Smith, is maintaining the six replicated aquaponcis systems and Mexcian scud tubs. A gravel base for the building containing the black soldier fly rearing systems (portapods) has be constructed. The BSFL rearing systems have been delivered which will help make certain that the building can accommadate these units. The 10' x 10' custom building will be delivered in May 2023. 4) Evaluate the use of Plant Growth Promoting Microorganisms (PGPM) in aquaponic media bed systems.** Nothing to report. B) Critical Extension Workshop 1: Basic and Advanced production The PowerPoint program has been developed. Videos are being filmed to increase the capacity to engage the audience. Workshop 2: Community/STEM Implications Nothing to report The three factsheets are listed below: Factsheet 1: System Design and Operation Drafts fact sheets are being editted. Factsheet 2: Waste Management Drafts fact sheets are being editted. Factsheet 3: Improving Profitability of Aquaponics Drafts fact sheets are being editted. C) Continuation Plan Tours of the aquponics facilitites occur ever week for a wide variety of audiences of all ages.

      Publications