Progress 03/01/24 to 02/28/25
Outputs
Target Audience:The DAVN is a framework to coordinate and standardize plant disease diagnostic assay development and validation research, and develop and deploy a series of tools and resources to the plant pathology community. Extension and outreach efforts engage the plant protection community, solicit feedback from participants, users, and engage industry stakeholders to raise awareness of diagnostic method development and validation resources available in the DAVN web portal. DAVN Communications and Outreach products housed in the DAVN portal publicize news, events, and products of DAVN. More than 80 scientists have opted to receive news about DAVN and get involved in the network. Outreach and communications efforts are essential to engaging the widest possible cohort of scientists in the DAVN effort, which are: 1. Engage scientists within these agencies/associations: Industry (seed, horticulture, vegetable, support services, and private labs) such as Agdia and Driscoll's 2. US Culture Collection Network 3. National Plant Diagnostic Network 4. National Clean Plant Network 5. National Seed Health Testing Association 6. National Plant Board 7. USDA-APHIS-Plant Protection and Quarantine 8. North American Plant Protection Organization Changes/Problems:Change of Project Director effective January 1, 2025 as PI Cardwell retired. Dr. Sharma is the new PI who was previously the CoPI of this project. What opportunities for training and professional development has the project provided?We conducted a full-day workshop at the Plant Health conference 2024 on July 27th2024 at Memphis, TN. The event was targeted at the Early Career Professionals and Graduate Students. The project waived the registration fees for the first 30 registrants from these committees. The workshop ran in full capacity (with a waiting list), indicating high interest from the plant pathology community. The participants were provided with the mock datasets and designed experimental plans to train and test the validation calculators developed by DAVN. All the presentations are uploaded to the DAVN Knowledge Center. We conducted a workshop to train the participants in how to develop diagnostic assays using HTS data using Microbe Finder technology on May 6-8 at Oklahoma State University, Stillwater, OK. We trained 3 postdocs, 3 grad students, and 4professionals. DAVN webinar series highlighted new and upcoming molecular techniques that can be applied to diagnostics (recordings available in DAVN web portal). We had 746 registrants and an average of 100 participants attending eachwebinar. During this project tenure, 3 undergraduate students, 2 graduate students, and apostdochave been trained, while another postdoc isbeing trained. How have the results been disseminated to communities of interest?Publications: Sharma, P., Cardwell, K., and Harmon, C. L. 2025. Diagnostic Assay Validation Network (DAVN) Progress and Evolution. PhytoFrontiers™ https://doi.org/10.1094/PHYTOFR-11-24-0123-FI Nascimento, D. M., Sharma, P., and Luster D. L. 2025 A 2023-2024 Literature Review of Validation Standards and Diagnostic Accuracy. PhytoFrontiers™ https://doi.org/10.1094/PHYTOFR-12-24-0132-FI Martin, F. N., Batuman, O., Luster, D. G., Miles, T. D., Miles, Rivera, Y Sharma, P. Geiser, D. and Cardwell, K. 2025. How Controls Improve Diagnostic Assay Performance: Hitchhikers Guide to Diagnostic Assay Controls. PhytoFrontiers™ https://doi.org/10.1094/PHYTOFR-10-24-0118-FI Conferences Sharma, P. presented a poster "Diagnostic Assay Validation Network Year 2: Validation tools and resources for better diagnostics" in Plant Health 2024 at Memphis, TN July 27-31, 2024 Sharma, P. presented a poster entitled "Update on Diagnostic Assay Validation Network Validation Tools and Resources" in National Plant Diagnostic Network National meeting at Portland, ME Sep 9-11, 2024 Batuman, O. Integrated Pest Management of Citrus and Tomato Pathogens in Florida. International Invited Talk; Applied Biosciences, Macquarie University, Sydney, Australia; March 26, 2025. Batuman, O. Integrated Pest Management of Citrus and Tomato Pathogens in Florida. SWFREC Field Day; Immokalee, FL; April 10, 2025. Batuman, O. My Pathology Program Overview. Multiple presentations at local meetings, including the SWFREC Faculty, Citrus Advisory Board, and Vegetable Advisory Board, February-May, 2025; Immokalee, FL What do you plan to do during the next reporting period to accomplish the goals?Publish the full issue of DAVN based focus issue - Diagnostic Assay Development and Validation: The Science of Getting It Right II (apsnet.org) Finish standardizing the protocols for the use of internal controls and submit a manuscript on this objective project with the intention of submitting it to Phytopathology. Update the NA database by adding data from peer reviewed publications from 2022-2025. Finish plugging in data for the Controls database by adding information from peer reviewed publications on the diagnostic assay development of Stramenopiles, Fungi, and Nematodes. Optimize RT-PCR detection protocolforOrthotospoviruses DAVN poster to be presented in the upcoming APS conference (Aug 2-6, 2025 Honolulu, HI) We are currently inviting a select pilot set of manuscripts that will be manually configured to fit the template, and they will be hosted on the DAVN site.We will not be using Manuscript Central for this activity.
Impacts
What was accomplished under these goals?
Diagnostic Assay Validation Network or the DAVN portal is housed within APS (https://www.apsnet.org/DAVN). DAVN has been created to provide access to strengthen, support, and develop tools and resources that can be utilized by the plant pathology community, especially assay developers, validators, and users in the diagnostic labs.The features that are currently available: • The nucleic acid extraction techniques database (https://www.apsnet.org/DAVN/Lists/NucleicAcidExtraction/Allitemsg.aspx) is searchable and includes information based on 115 peer-reviewed publications from 2011 - 2022 across 6 taxa: Bacteria, Fungi, Stramenopiles, Nematodes, Virus, and Viroids. The database provides comprehensive information on NA extraction and assay mix kits, and equipment used, depending upon the host, matrix, and the purpose of the study. • The searchable assay controls database (https://www.apsnet.org/DAVN/Lists/Controls/Allitemsg.aspx) which includes information from 55 peer reviewed publications from 2011 - 2022 across 3 taxa: Bacteria, Virus, and Viroids. The database comprises information on the control type, control target region, specificity level at which it was used, and pathogen target depending upon the pathogen, host, and assay method used in the studies. For a better understanding of the terminology and acronyms used by the database, an assay control terminology and description of rationale table has also been provided. Additionally, a proposal to further develop the database of validated and published exogenous and endogenous controls was submitted by DAVN research committee members to PPA 7721. It has been approved. This new information will be integrated into the controls database. • A validation framework summarizing the validation tier overview, which illustrates the hierarchical tiers for assay validation, theworkflow of assay development and validation, which includes the details of validation workflows, and a table ofkey terminologythat defines each of the key attributes to be assessed by the validation procedure.? This is presented in the Tools section https://www.apsnet.org/DAVN/Tools/Assay-Development-Validation/Pages/default.aspx • Assay performance metric validation calculators with information about the statistical algorithms behind each calculator, links to the calculators, and how to report the performance metric in published protocols are published and beta tested. It currently has calculators for sensitivity, precision, and accuracy at https://www.apsnet.org/DAVN/Tools/Calculators/Pages/default.aspx DAVN Communications and Outreach products housed in the DAVN portal publicize news, events, and products of DAVN. More than 80 scientists have opted in to receive news about DAVN and get involved in the network. The information is provided in the DAVN Knowledge center at https://www.apsnet.org/DAVN/Knowledge/Pages/default.aspx A DAVN based focus issue was published in 2023 in PhytoFrontiers https://apsjournals.apsnet.org/toc/phytofr/3/1. It had recommended diagnostic assay validation guidelines for the authors and reviewers. In total, 26 articles were accepted, including the 6 foundational perspective articles on DAVN. We also had publications from international researchers. The whole issue has been downloaded close to 674 times, and the independent articles have been downloaded more than 50,000 times. The articles in this issue have received 152 citations according to Google Scholar. The success of that issue indicated a demand for a publication outlet for diagnostic assay development and validation research. The second focus issue was announced (Diagnostic Assay Development and Validation: The Science of Getting It Right II (apsnet.org) in 2024. It will be published in June/July 2025. We received close to 20 articles, of which 16 have been confirmed. Ten articles have been published and two are in first looks, the articles have been downloaded more than 2000 times already. • In addition, publication guidelines were proposed to APS Press Editorial Board and these guidelines are live in the Information to Authors page under the section Instructions to Authors of Diagnostic Assay Validation Papers (https://apsjournals.apsnet.org/page/authorinformation) • DAVN webinar series highlighted new and upcoming molecular techniques that can be applied to diagnostics (recordings available in DAVN web portal). Five webinars drew 746 registrants and averaged nearly 100 participants each; remarkably, dozens of non-APS members paid to participate, an indication of the value of the DAVN resources and a possible contribution towards the sustainability conversation. All the webinars are available on demand in the DAVN Knowledge center. Additional assets include tools for collaboration such as directories and participant registries. In our Research objectives on the use of Internal controls in diagnostic assays, atotal of ten endogenous positive internal controls were selected from the literature based on their suitability and broad applicability, conducting a preliminary screening to confirm their performance. Seven endogenous PICs were successful, and their sensitivity and specificity were tested in single plex with 64 plant species. Two endogenous PICs, '18S-uni' and 'FMPI (cox1)', showed the highest sensitivity and broadest plant coverage. While 18S-uni covered all plants, FMPI did not amplify gymnosperm taxa consistently. Both endogenous PICs were tested in multiplex with a publishedPhytophthoraspp. assay. 18S-uni high sensitivity caused water control contamination, affecting pathogen detection. FMPI was more stable, leading to further evaluation under various conditions. Sequence alignments revealed why FMPI failed to amplify gymnosperms and alternative primer, and probe sets were designed for improved amplification for those taxa. This research provides a foundation for standardizing endogenous PICs in diagnostic PCR assays for plant pathogen detection. Another Internal control is theplant actin gene, specific primers were designed and tested on several DNA/RNA samples from a limited number of plants. This PIC was found to be plant specific. Further validation of the assay is in progress. In the other research objective, designed six new pairs of degenerate primers targeting conserved gene sequences of orthotospovirus, but results were ambiguous. The experiments are being repeated to optimize a protocol that will be more reliable in detecting orthotospovirus.
Publications
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2025
Citation:
Martin, F. N., Batuman, O., Luster, D. G., Miles, T. D., Miles, Rivera, Y Sharma, P. Geiser, D. and Cardwell, K. 2025. How controls improve diagnostic assay performance: Hitchhikers guide to diagnostic assay controls. PhytoFrontiers 0:0. https://doi.org/10.1094/PHYTOFR-10-24-0118-FI
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2025
Citation:
Sharma, P., Cardwell, K., and Harmon, C. L. 2025. Diagnostic Assay Validation Network (DAVN) progress and evolution. PhytoFrontiers 0:0. https://doi.org/10.1094/PHYTOFR-11-24-0123-FI
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2025
Citation:
Nascimento, D. M., Sharma P., and Luster, D. G. 2025. A 20232024 Literature review of validation standards and diagnostic accuracy. PhytoFrontiers 0:0. https://doi.org/10.1094/PHYTOFR-12-24-0132-FI
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Progress 03/01/23 to 02/29/24
Outputs
Target Audience:The DAVN is a framework to coordinate and standardize plant disease diagnostic assay development and validation research and develop and deploy a series of tools and resources to the plant pathology community. Extension and outreach efforts engage the plant protection community, solicit feedback from participants, users, and engage industry stakeholdersto raise awareness of diagnostic method development and validation resources available in the DAVN web portal. DAVN Communications and Outreach products housed in the DAVN portal publicize news, events, and products of DAVN. More than 80 scientists have opted in to receive news about DAVN and get involved in the network. Outreach and communications efforts are essential to engaging the widest possible cohort of scientists to the DAVN effort. Objectives: Engage scientists within these agencies/associations: Industry (seed, horticulture, vegetable, support services, and private labs) Agdia, Driscoll's, BallHort, etc) US Culture Collection Network National Plant Diagnostic Network National Clean Plant Network National Seed Health Testing Association National Plant Board USDA-APHIS-Plant Protection and Quarantine North American Plant Protection Organization Changes/Problems:No major changes have been encountered. Milestones for the objectives are being met. What opportunities for training and professional development has the project provided?In the summer of 2024, an APS-DAVN workshop will be held at the Plant Health meeting in Memphis. The event is being advertised by APS to the Early Career Professionals and Graduate Student committees. The project is waiving registration fees for the first 30 registrants from these committees. An additional workshop will be held on-line later in 2024. The DAVN webinar series was free to all APS members, and are available recordings for everyone in the DAVN webportal. Many professors told us that they dedicated lecture periods to air these webinars to their students to expose them to the prinicples of diagnostic assay development and validation. How have the results been disseminated to communities of interest?DAVN Communications and Outreach products housed in the DAVN portal publicize news, events, and products of DAVN. More than 80 scientists have opted in to receive news about DAVN and get involved in the network. Publications - A DAVN based focus issue was published in 2023 in PhytoFrontiers. It had recommended diagnostic assay validation guidelines for the authors and reviewers. The focus issue attracted >30 articles/perspectives/reviews. In total, 26 articles were accepted including the 6 foundational perspective articles on DAVN. We also had publications from international researchers. The whole issue has been downloaded close to 400 times, the independent articles have been downloaded more than 34,000 times. The articles in this issue have received 84 citations according to Google Scholar. The success of that issue indicated a demand for a publication outlet for diagnostic assay development and validation research. So a new focus issue has been announced (Call for Papers: Diagnostic Assay Development and Validation: The Science of Getting It Right II (apsnet.org) In addition, publication guidelines were proposed to APS Press Editorial Board and these guidelines are live in the Information to Authors page under the section Instructions to Authors of Diagnostic Assay Validation Papers (https://apsjournals.apsnet.org/page/authorinformation) DAVN webinar series highlighted new and upcoming molecular techniques that can be applied to diagnostics (recordings available in DAVN web portal). Five webinars drew 746 registrants and averaged nearly 100 participants each; remarkably, dozens of non-APS members paid to participate, an indication of the value of the DAVN resources and a possible contribution towards the sustainability conversation. Additional assets include tools for collaboration such as directories and participant registries, standard assay validation terminology and workflow for different tiers of validation, validation statistical calculators, and guidelines for publication of diagnostic assay development and validation research. What do you plan to do during the next reporting period to accomplish the goals? The success of the initial PhytoFrontiers focus issue indicated a demand for a publication outlet for diagnostic assay development and validation research. So a new focus issue has been announced (Call for Papers: Diagnostic Assay Development and Validation: The Science of Getting It Right II (apsnet.org), with a submission deadline of the end of August 2024 and on-ine publication in Reb 2025. At the 2024 APS meeting (July, 27 2024) in Memphis Tennessee, DAVN is presenting a day-long workshop on the network products, and opportunities. An industry focus group will be hosted to participate in this workshop. Set up Manucscript Central template to convert diagnostic assay development and validation research journal articles into Work Instructions for diagnostic assay users. The authors will recieve a second publication DOI for the research, once the Work Instruction protocol has cleared review.
Impacts
What was accomplished under these goals?
The DAVN web-portal, being developed in partnership with the American Phytopathological Society (APS), provides access to diagnostic sciences assets, integrating validation research data, providing workflow, calculators, and community. These features are currently available: The nucleic acid extraction techniques database is searchable and includes information based on 115 peer reviewed publications since 2011 on NA extraction and assay mix kits, and equipment used depending upon the host, matrix and the purpose of the study. The searchable assay controls database presents definitions and data on controls available from an extensive literature search from the past decade. Additionally, a proposal to further develop the database of validated and published exogenous and endogenous controls was submitted by DAVN research committee members to PPA 7721. It has been approved. This new information will be integrated into the controls database. Framework, workflow and a glossary of assay development terminology are presented to promote research in tiers of validation related to fitness of purpose of an assay (Validation Tools (apsnet.org). Assay performance metric validation calculators with information about the statistical algorithms behind each calculator, links to the calculators and how to report the performance metric in published protocols are published and beta tested. DAVN Communications and Outreach products housed in the DAVN portal publicize news, events, and products of DAVN. More than 80 scientists have opted in to receive news about DAVN and get involved in the network. Publications - A DAVN based focus issue was published in 2023 in PhytoFrontiers. It had recommended diagnostic assay validation guidelines for the authors and reviewers. The focus issue attracted >30 articles/perspectives/reviews. In total, 26 articles were accepted including the 6 foundational perspective articles on DAVN. We also had publications from international researchers. The whole issue has been downloaded close to 400 times, the independent articles have been downloaded more than 34,000 times. The articles in this issue have received 84 citations according to Google Scholar. The success of that issue indicated a demand for a publication outlet for diagnostic assay development and validation research. So a new focus issue has been announced (Call for Papers: Diagnostic Assay Development and Validation: The Science of Getting It Right II (apsnet.org) In addition, publication guidelines were proposed to APS Press Editorial Board and these guidelines are live in the Information to Authors page under the section Instructions to Authors of Diagnostic Assay Validation Papers (https://apsjournals.apsnet.org/page/authorinformation) DAVN webinar series (see Other Products in this report) highlighted new and upcoming molecular techniques that can be applied to diagnostics (recordings available in DAVN web portal). Five webinars drew 746 registrants and averaged nearly 100 participants each; remarkably, dozens of non-APS members paid to participate, an indication of the value of the DAVN resources and a possible contribution towards the sustainability conversation. Additional assets include tools for collaboration such as directories and participant registries, standard assay validation terminology and workflow for different tiers of validation, validation statistical calculators, and guidelines for publication of diagnostic assay development and validation research.
Publications
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Progress 03/01/22 to 02/28/23
Outputs
Target Audience: To build a community of diagnostic researchers, regulators, industry members, and diagnostic professionals across silos of government, industry, and academia Achievements - Below is the list of work that has been done so far: A DAVN mailbox DAVN@scisoc.com and Twitter account (https://twitter.com/the_davn) has been set up and is actively publishing updates. We have also a link for the community to receive DAVN updates - https://forms.scisoc.org/davn-info/ DAVN was presented in the NPDN webinar (March 1, 2023), the recording has been posted in the DAVN portal - https://learnerresources.s3.eu-west-1.amazonaws.com/102661/learner_resource_uploads/108ee1cb1658c7f0cffc0f0b46/GPDN%20Webinar%20Series_03-01-2023.mp4?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIAQZVLRHER6MLZQEAI%2F20230717%2Feu-west-1%2Fs3%2Faws4_request&X-Amz-Date=20230717T220857Z&X-Amz-Expires=30&X-Amz-SignedHeaders=host&X-Amz-Signature=0769105fd477b40a819f37ed63acd840630b39248b325da9bd4252a3aed5d2d2 Publication guidelines proposed to APS Press Editorial Board and these guidelines are already live in the Information to Authors page under the section Instructions to Authors of Diagnostic Assay Validation Papers (https://apsjournals.apsnet.org/page/authorinformation) DAVN was featured on the Plantopia podcast episode titled "Collecting Samples, Bridging Silos: Plant Diagnostics and the NPDN," which was broadcasted on April 27th, 2023 - https://aphid.fireside.fm/d/1437767933/43a22cf8-a632-4de5-8c29-0a5c6de955d8/459e9acc-25fa-478f-ba7f-922e9573756b.mp3 On May 9th, 2023, DAVN was showcased during the Western Extension Research Activity WERA 20 meeting, which took place from May 9-11, 2023. Changes/Problems:Dr Frank Martin, USDA ARS Salinas, requested to withdraw from the project to be replaced by his previous Post Doc, Timothy Miles at Michigan State University, Dr Martin continues to advise the reserach group. Dr Judy Brown, U AZ, did not have time to dedicate to the DAVN project. She was replaced by Dr Ozgur Batuman at U FL. Dr Batuman is in the process of recruiting a Post Doctoral Fellow. What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest? Outreach committee - To build a community of diagnostic researchers, regulators, industry members, and diagnostic professionals across silos of government, industry, and academia Achievements - Below is the list of work that has been done so far: A DAVN mailbox DAVN@scisoc.com and Twitter account (https://twitter.com/the_davn) has been set up and is actively publishing updates. We have also a link for the community to receive DAVN updates - https://forms.scisoc.org/davn-info/ DAVN was presented in the NPDN webinar (March 1, 2023), the recording has been posted in the DAVN portal - https://learnerresources.s3.eu-west-1.amazonaws.com/102661/learner_resource_uploads/108ee1cb1658c7f0cffc0f0b46/GPDN%20Webinar%20Series_03-01-2023.mp4?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIAQZVLRHER6MLZQEAI%2F20230717%2Feu-west-1%2Fs3%2Faws4_request&X-Amz-Date=20230717T220857Z&X-Amz-Expires=30&X-Amz-SignedHeaders=host&X-Amz-Signature=0769105fd477b40a819f37ed63acd840630b39248b325da9bd4252a3aed5d2d2 Publication guidelines proposed to APS Press Editorial Board and these guidelines are already live in the Information to Authors page under the section Instructions to Authors of Diagnostic Assay Validation Papers (https://apsjournals.apsnet.org/page/authorinformation) DAVN was featured on the Plantopia podcast episode titled "Collecting Samples, Bridging Silos: Plant Diagnostics and the NPDN," which was broadcasted on April 27th, 2023 - https://aphid.fireside.fm/d/1437767933/43a22cf8-a632-4de5-8c29-0a5c6de955d8/459e9acc-25fa-478f-ba7f-922e9573756b.mp3 On May 9th, 2023, DAVN was showcased during the Western Extension Research Activity WERA 20 meeting, which took place from May 9-11, 2023. What do you plan to do during the next reporting period to accomplish the goals?Next step actions: 1. DAVN poster to be presented in the upcoming APS conference (Aug-12-16, 2023 Denver, CO) 2. APS to present DAVN in International Conference on Plant Pathology (ICPP) conference in (Aug-20-25, 2023 Lyon, France) 3. Schedule list of webinars on DAVN Fall/Winter 2023-2024 3. Plug-in the validation statistical calculators in the DAVN webpage 4. Plug-in the extraction techniques database in the DAVN portal 5. Develop Pitch Decks for Researchers, Assay Developers and Validation Researchers; Industry and Government Stakeholders
Impacts
What was accomplished under these goals?
DAVN WEBportal Site Map Home Page - Welcome the audience to the DAVN homepage. You can access more information by visiting DAVN.org or following us on Twitter. 1. What DAVN Does - This section addresses what DAVN is about and what does it consist of and all of its activities. 2. DAVN Assets - Explore the information DAVN has to offer in the form of databases for diagnostic assay development (extraction techniques, universal internal controls, positive controls, primers, probes, and genomic markers); tools for collaboration such as directories and participant registries; validation tools and standards; and a repository for validated techniques, methods, and work instructions. 3. Research sandbox - Discover DAVN-relevant research by checking on the Research sandbox which will also have project management Gantt charts. We will be happy to hear your feedback, and everyone is welcome to interact with the research team. 4. Communications and Outreach - This section emphasizes the outreach, the importance of staying connected with DAVN and be informed about the latest developments in DAVN by following us on DAVN.org, and @the.davn in Twitter. It provides links to publications, training, essays, and webinars. In addition, please explore our network through DAVN directories and registries. ?
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
The Need and a Vision for a Diagnostic Assay Validation Network.
Kitty F. Cardwell, Carrie L. Harmon, Douglas G. Luster, James P. Stack, Aimee M. Hyten, Poonam Sharma, and Mark K. Nakhla PhytoFrontiers" 2023 3:1, 9-17
https://doi.org/10.1094/PHYTOFR-05-22-0056-FI
Downloaded 729 times
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
State of the Field of Plant Pathogen Diagnostic Assay Development and Validation Poonam Sharma and Douglas G. Luster PhytoFrontiers" 2023 3:1, 18-22
https://doi.org/10.1094/PHYTOFR-05-22-0054-FI
Downloaded 306 times
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Terminology and Guidelines for Diagnostic Assay Development and Validation: Best Practices for Molecular Tests Deborah Groth-Helms, Yazm�n Rivera, Frank N. Martin, Mohammad Arif, Poonam Sharma, and Lisa A. Castlebury PhytoFrontiers" 2023 3:1, 23-35
https://doi.org/10.1094/PHYTOFR-05-22-0059-FI
Downloaded 459 times
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Knowledge Gaps, Research Needs, and Opportunities in Plant Disease Diagnostic Assay Development and Validation David M. Geiser, Frank N. Martin, Andres S. Espindola, Judith K. Brown, Terrence H. Bell, Yinong Yang, and Seogchan Kang PhytoFrontiers" 2023 3:1, 51-63
https://doi.org/10.1094/PHYTOFR-05-22-0057-FI
Downloaded 556 times
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
5. Communications Ecosystem to Support the Assay Validation Community: A Concept James P. Stack and Kitty F. Cardwell PhytoFrontiers" 2023 3:1, 36-42
https://doi.org/10.1094/PHYTOFR-05-22-0055-FI
Downloaded 204 times
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Standards of Diagnostic Validation: Recommendations for Reference Collections Carrie L. Harmon, Lisa A. Castlebury, Kyria Boundy-Mills, Kirk D. Broders, Aimee M. Hyten, Jonathan L. Jacobs, Victoria K. Knight-Connoni, Dimitre Mollov, Marco A. Riojas, and Poonam Sharma PhytoFrontiers" 2023 3:1, 43-50
https://doi.org/10.1094/PHYTOFR-05-22-0050-FI
Downloaded 490 times
- Type:
Books
Status:
Published
Year Published:
2023
Citation:
Carwell, Harmon, Stack, and Sharma, eds. Special Focus Issue - Diagnostic
Assay Development and Validation: The Science of Getting It Right. Phytofrontiers Vol3, No. 1. https://doi.org/10.1094/PHYTOFR-3-1
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