Progress 01/01/22 to 11/14/24
Outputs
Target Audience:Over the duration of the project I have worked with the following target audiences: 1. Middle and High School Students: I have mentored middle and high school students as part of the virtual science club as the Natural Sciences mentor for the years 2022 and 2023. As the Natural Sciences mentor I helped middle and high school students from across the state of Tennessee develop science fair projects related to animal and plant sciences and microbiology. As part of this process, I recruited a senior from a local Knoxville high school tand together we developed a science fair project that was incorporated into my dissertation and was also presented at the 2022 Southern Appalachian Science and Engineering Fair where the student received a Certificate of Excellene for the senior division Microbiology category and 3rd place in the overall Senior Division. 2. Undergraduate Students: Over the years 2022 and 2023 I mentored five University of Tennessee undergraduate students in majors related to Plant Sciences, Forestry and Natural Resources, and Neuroscience. These students participated in research related to biological and chemical mangement development for Thousand Cankers Disease. All five students presented their research at either the 2022 or 2023 University of Tennessee Undergraduate Research Symposiaand will be included as co-authors on the resulting peer-reviewed publications. I also mentored 12 female undergraduate students as part of the 2022 and 2023 Bioninformatics, Genetics, and Genomics REEU hosted at the University of Tennessee. As part of this program, I taught students R basics and how to process amplicon sequence data generated using Next-Generation Sequencing technologies. Two of the twelve students aided me in analyzing data from the 2022 drought study and will serve as co-authors on the resulting peer-reviewed publication. These students also presented their reserach at the University of Tennessee Summer Research Symposium. 3. Graduate Students: Over the course of the funding period, I peer mentored five graduate students in the Department of Entomology and Plant Pathology at the University of Tennessee. As part of my peer-mentoring I taught students the basics of plant pathology including Koch's postulates. I also aided students with experimental design and taught students RNA and DNA extractions. I also taught students how to process amplicon sequence data generated using Next-Generation Sequencing technologies. Finally, I served a first co-author on a peer-reviewed manuscript published in PeerJ and am a co-author on at least 3 other mansucripts that are in-preparation for submission to peer-reviewed journals. 4. Visiting Scholars:Over the course of the funding period, I mentored two visiting scholars. One was a post-doctoral research from Iran and the other was an undergraduate student from Honduras studying agricultural sciences. As part of my mentoring duties, I taught both visiting scholars how to measure plant physiological variables related to drought stress, how to collect plant tissues for RNA and DNA extractions, and how to complete Koch's postulates. A peer-reviewed disease note was published in Plant Disease as a product of this mentoring. Both scholars will also serve as co-authors on two manuscripts that are currently being prepared for submission to peer-reviewed journals. Changes/Problems:In regards to Training/Career Development Goal 3: Develop teaching skills by teaching high school students the basics of plant pathology as part of the Pre-College Upward Bound Program and teaching undergraduate students the basics of microbiome data analysis as part of the USDA BiGG Data REEU. Additionally, I will develop and teach a 3 credit graduate level course on microbiome data analysis and complete the UT Graduate School's Best Practices in Teaching. I was unable to teach the 3-credit graudate level course on microbiome data analysis in the Fall of 2022 due to changes in UT policy. However, I did develop and teach the course in Fall of 2021 and complete UT Graduate School's Best Practices in Teaching prior to the start of the funding. What opportunities for training and professional development has the project provided?1. In summer 2022, I conducted a greenhouse experiment that addresses research objective 1 (Quantify G. morbida virulence in drought-stressed trees and identify responses of the J. nigra phytobiome to drought) and objective 2 (Identify responses of host/pathogen interactions to drought in G. morbida infected J. nigra trees). As part of this research I learned how to measure plant physiological variables related to drought stress and improved my project management and troubleshooting skills. Additionally, I established a collaboration with Scott Gula, a PhD student at Purdue University who will characterize the chemical profile of eastern black walnut wood exposed to drought using bark and phloem samples collected during this study. 2. I participated as a mentor in the virtual science club in 2022 and 2023. As part of this process, I have mentored middle school and high school students, helping them develop ideas for their science fair project. Two of these students received Certificates of Excellence at the Southern Appalachian Science and Engineering Fair. 3. In Summers 2022, I taught a visiting scholar and undergraduate student from Honduras, the basics of plant pathology and how. to conduct Koch's postulates. As a result of my teaching efforts, they completed Koch's postulates, which resulted in a first report publication in Plant Disease. 4. I attended the 2022 and 2023 American Phytopathological Society Plant Health conferences where I networked with other Plant Health professionals and presented posters describing the results of my original research projects. 5. I mentored 12female undergraduate students as part of the 2022 and 2023 USDA BiGG Data REEU. I taught these students how to process amplicon sequences generated from next-generation sequencing technologiesand perform statistical analyses in R. As part of this mentoring, two of these undergraduate students will serve as co-authors on the manuscript describing the findings of the drought study that will be submitted for publication in a peer-reviewed journal. How have the results been disseminated to communities of interest?As part of my training and development, I presented results of my dissertation research at the following conferences: 1. American Phytopathological Society Plant Health 2022 annual Conference 2.The Role of Microbiomes in Forest Disease, Regeneration, and Biodiversity Webinar, hosted by the American Phytopathological Society 2022 3.American Phytopathological Society Plant Health 2023annual Conference I was also a co-author on a book chapter and an extension document published in 2022 and two peer-reviewed publications publised in 2023. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Training/Career Development: 1. I improved my critical thinking skills through the completion of the proposed original research project that examined the effects of drough stress on the severity of Thousand Cankers Disease. The greenhouseexperiment was completed in the summer of 2022 and all lab work required for sample processing was completed in 2023. During the completion of lab work, I had to troubleshoot DNA and RNA extraction methodologies to increase the quality and purity of RNA and DNA for use in RNASeq and amplicon sequencing using Next-Generation sequencing technologies. I also had to troubleshoot during data analysis, particularly interpreting and correcting errors in bash and R scripts for sequencing processing and statistical analyses. 2.I have mentored middle and high school students as part of the virtual science club as the Natural Sciences mentor for the years 2022 and 2023. As the Natural Sciences mentor I helped middle and high school students from across the state of Tennessee develop science fair projects related to animal and plant sciences and microbiology. As part of this process, I recruited a senior from a local Knoxville high school tand together we developed a science fair project that was incorporated into my dissertation and was also presented at the 2022 Southern Appalachian Science and Engineering Fair (SASEF) where the student received a Certificate of Excellence for the senior division Microbiology category and 3rd place in the overall Senior Division. 4. Over the course of the funding period I presented my research to other plant health professionals at the 2022 and 2023 American Phytopathological Society Plant Health annual conferences. I also helped undergraduate and graduate students prepare posters for presentations at local and national conferences. Original Research Project 1.In summer 2022, we conducted a greenhouse experiment to characterize the effects of drought on the virulence of Geosmithia morbida in drought-stressed black walnut trees. We innouclated 78 trees with G. morbida in 4 different positions along each stem. The study lasted for a total of 20 days with samples collected at day 3, day 8, day 14, day 15, and day 20 post-inoculation. A total of 24 trees were maintained as well-watered controls and 40 were used as drought stressed. An addition 24 trees were used as a rewatered treatment, which were exposed to drought until day 14 postinoculation and then rewatered to test the effects of supplemental irrigation on the virulence and host-pathogen interaction. We determined that cankers were larger in drought stressed trees at days 14, 15, and 20 post-inoculation. Additionally, we determined that gravimetic transpiration rates and leaf water potential were generally lower drought stressed trees compared to well-watered controls. In 2023, I completed DNA extractions which was used for amplicon sequencing of fungal and bacterial communities.WIth the help of two undergraduate students from the BIGG REEU we process amplicon sequencing data and determined that both fungal and archaeal/bacterial communities shifted in response to drought treatments indicating that environmental stressors interact with G. morbidato further perturb the host microbiome. 2. In 2023, I completed RNA extractions from phloem tissues of drought stressed and well-watered trees. Our preliminary findings indicate that the gene expressions of drought stressed and well-watered G. morbida infected trees differ. Additionally, we found that the G. morbida transcriptome differs as a result of host drought stress. I am currently in the process of identifying and annotating what genes are shifting in expression as a result of drought stress and the relevance of these genes in host/pathogen interactions.
Publications
- Type:
Other Journal Articles
Status:
Published
Year Published:
2023
Citation:
Kapoor B, Onufrak A, Klingeman III W, DeBruyn JM, Cregger MA, Willcox E, Trigiano R, Hadziabdic D. Signatures of prescribed fire in the microbial communities of Cornus florida are largely undetectable five months post-fire. PeerJ. 2023 Aug 24;11:e15822.
- Type:
Other Journal Articles
Status:
Published
Year Published:
2023
Citation:
Reyes AM, Smith ZC, Onufrak AJ, Pietsch GM, Ony M, Odoi ME, Khodaei S, Smallwood CJ, Ginzel M, Klingeman WE, Hadziabdic D. First Report of Bot Canker (Diplodia corticola) in Pin Oak (Quercus palustris) in Tennessee. Plant Disease. 2023 Dec 1;107(12):4019.
- Type:
Theses/Dissertations
Status:
Accepted
Year Published:
2023
Citation:
Characterization of Plant-Microbial Interactions in the Thousand Cankers Disease Complex using Amplicon Sequencing, Transcriptomics, and Genomics
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2023
Citation:
Onufrak A.J., Pietsch G., Givens S., Klingeman D., Klingeman W., Cregger M., Walters K., Shekoofa S., Hadziabdic D. (2023) A not so dry subject: The responses of microbial communities associated with Geosmithia morbida infected Juglans nigra trees to drought stress. American Phytopathological Society Conference, Denver, CO (August 2023). Oral Presentation.
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Progress 01/01/23 to 12/31/23
Outputs
Target Audience:Over the duration of the project I have worked with the following target audiences: 1. Middle and High School Students: I have mentored middle and high school students as part of the virtual science club as the Natural Sciences mentor for the years 2022 and 2023. As the Natural Sciences mentor I helped middle and high school students from across the state of Tennessee develop science fair projects related to animal and plant sciences and microbiology. As part of this process, I recruited a senior from a local Knoxville high school tand together we developed a science fair project that was incorporated into my dissertation and was also presented at the 2022 Southern Appalachian Science and Engineering Fair where the student received a Certificate of Excellene for the senior division Microbiology category and 3rd place in the overall Senior Division. 2. Undergraduate Students: Over the years 2022 and 2023 I mentored five University of Tennessee undergraduate students in majors related to Plant Sciences, Forestry and Natural Resources, and Neuroscience. These students participated in research related to biological and chemical mangement development for Thousand Cankers Disease. All five students presented their research at either the 2022 or 2023 University of Tennessee Undergraduate Research Symposia and will be included as co-authors on the resulting peer-reviewed publications. I also mentored 12 female undergraduate students as part of the 2022 and 2023 Bioninformatics, Genetics, and Genomics REEU hosted at the University of Tennessee. As part of this program, I taught students R basics and how to process amplicon sequence data generated using Next-Generation Sequencing technologies. Two of the twelve students aided me in analyzing data from the 2022 drought study and will serve as co-authors on the resulting peer-reviewed publication. These students also presented their reserach at the University of Tennessee Summer Research Symposium. 3. Graduate Students: Over the course of the funding period, I peer mentored five graduate students in the Department of Entomology and Plant Pathology at the University of Tennessee. As part of my peer-mentoring I taught students the basics of plant pathology including Koch's postulates. I also aided students with experimental design and taught students RNA and DNA extractions. I also taught students how to process amplicon sequence data generated using Next-Generation Sequencing technologies. Finally, I served a first co-author on a peer-reviewed manuscript published in PeerJ and am a coauthor on at least 3 other mansucripts that are in-preparation for submission to peer-reviewed journals. 4. Visiting Scholars: Over the course of the funding period, I mentored two visiting scholars. One was a post-doctoral research from Iran and the other was an undergraduate student from Honduras studying agricultural sciences. As part of my mentoring duties, I taught both visiting scholars how to measure plant physiological variables related to drought stress, how to collect plant tissues for RNA and DNA extractions, and how to complete Koch's postulates. A peer-reviewed disease note was published in Plant Disease as a product of this mentoring. Both scholars will also serve as co-authors on twomanuscripts that are currently being prepared for submission to peer-reviewed journals. Changes/Problems:In regards to Training/Career Development Goal 3: Develop teaching skills by teaching high school students the basics of plant pathology as part of the Pre-College Upward Bound Program and teaching undergraduate students the basics of microbiome data analysis as part of the USDA BiGG Data REEU. Additionally, I will develop and teach a 3 credit graduate level course on microbiome data analysis and complete the UT Graduate School's Best Practices in Teaching. I was unable to teach the 3-credit graudate level course on microbiome data analysis in the Fall of 2022 due to changes in UT policy. However, I did develop and teach the course in Fall of 2021 and complete UT Graduate School's Best Practices in Teaching prior to the start of the funding. What opportunities for training and professional development has the project provided?1. In summer 2022, I conducted a greenhouse experiment that addresses research objective 1 (Quantify G. morbida virulence in drought-stressed trees and identify responses of the J. nigra phytobiome to drought) and objective 2 (Identify responses of host/pathogen interactions to drought in G. morbida infected J. nigra trees). As part of this research I learned how to measure plant physiological variables related to drought stress and improved my project management and troubleshooting skills. Additionally, I established a collaboration with Scott Gula, a PhD student at Purdue University who will characterize the chemical profile of eastern black walnut wood exposed to drought using bark and phloem samples collected during this study. 2. I participated as a mentor in the virtual science club in 2022 and 2023. As part of this process, I have mentored middle school and high school students, helping them develop ideas for their science fair project. Two of these students received Certificates of Excellence at the Southern Appalachian Science and Engineering Fair. 3. In Summers 2022, I taught a visiting scholar and undergraduate student from Honduras, the basics of plant pathology and how. to conduct Koch's postulates. As a result of my teaching efforts, they completed Koch's postulates, which resulted in a first report publication in Plant Disease. 4. I attended the 2022 and 2023 American Phytopathological Society Plant Health conferences where I networked with other Plant Health professionals and presented posters describing the results of my original research projects. 5. I mentored 12 female undergraduate students as part of the 2022 and 2023 USDA BiGG Data REEU. I taught these students how to process amplicon sequences generated from next-generation sequencing technologies and perform statistical analyses in R. As part of this mentoring, two of these undergraduate students will serve as co-authors on the manuscript describing the findings of the drought study that will be submitted for publication in a peer-reviewed journal. How have the results been disseminated to communities of interest?As part of my training and development, I presented results of my dissertation research at the following conferences: 1. American Phytopathological Society Plant Health 2022 annual Conference 2. The Role of Microbiomes in Forest Disease, Regeneration, and Biodiversity Webinar, hosted by the American Phytopathological Society 2022 3. American Phytopathological Society Plant Health 2023 annual Conference I was also a co-author on a book chapter and an extension document published in 2022 and two peer-reviewed publications publised in 2023. What do you plan to do during the next reporting period to accomplish the goals?I plan to publish the remaining three chapters of my dissertation and the results of my original research project in peer-reviewed journals.
Impacts
What was accomplished under these goals?
Training/Career Development: 1. I improved my critical thinking skills through the completion of the proposed original research project that examined the effects of drough stress on the severity of Thousand Cankers Disease. The greenhouse experiment was completed in the summer of 2022 and all lab work required for sample processing was completed in 2023. During the completion of lab work, I had to troubleshoot DNA and RNA extraction methodologies to increase the quality and purity of RNA and DNA for use in RNASeq and amplicon sequencing using Next-Generation sequencing technologies. I also had to troubleshoot during data analysis, particularly interpreting and correcting errors in bash and R scripts for sequencing processing and statistical analyses. 2. I have mentored middle and high school students as part of the virtual science club as the Natural Sciences mentor for the years 2022 and 2023. As the Natural Sciences mentor I helped middle and high school students from across the state of Tennessee develop science fair projects related to animal and plant sciences and microbiology. As part of this process, I recruited a senior from a local Knoxville high school tand together we developed a science fair project that was incorporated into my dissertation and was also presented at the 2022 Southern Appalachian Science and Engineering Fair (SASEF) where the student received a Certificate of Excellence for the senior division Microbiology category and 3rd place in the overall Senior Division. 3. Over the course of the funding period I presented my research to other plant health professionals at the 2022 and 2023 American Phytopathological Society Plant Health annual conferences. I also helped undergraduate and graduate students prepare posters for presentations at local and national conferences. Original Research Project: 1. In summer 2022, we conducted a greenhouse experiment to characterize the effects of drought on the virulence of Geosmithia morbida in drought-stressed black walnut trees. We innouclated 78 trees with G. morbida in 4 different positions along each stem. The study lasted for a total of 20 days with samples collected at day 3, day 8, day 14, day 15, and day 20 post-inoculation. A total of 24 trees were maintained as well-watered controls and 40 were used as drought stressed. An addition 24 trees were used as a rewatered treatment, which were exposed to drought until day 14 postinoculation and then rewatered to test the effects of supplemental irrigation on the virulence and host-pathogen interaction. We determined that cankers were larger in drought stressed trees at days 14, 15, and 20 post-inoculation. Additionally, we determined that gravimetic transpiration rates and leaf water potential were generally lower drought stressed trees compared to well-watered controls. In 2023, I completed DNA extractions which was used for amplicon sequencing of fungal and bacterial communities. WIth the help of two undergraduate students from the BIGG REEU we process amplicon sequencing data and determined that both fungal and archaeal/bacterial communities shifted in response to drought treatments indicating that environmental stressors interact with G. morbida to further perturb the host microbiome. 2. In 2023, I completed RNA extractions from phloem tissues of drought stressed and well-watered trees. Our preliminary findings indicate that the gene expressions of drought stressed and well-watered G. morbida infected trees differ. Additionally, we found that the G. morbida transcriptome differs as a result of host drought stress. I am currently in the process of identifying and annotating what genes are shifting in expression as a result of drought stress and the relevance of these genes in host/pathogen interactions.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Kapoor B, Onufrak A, Klingeman III W, DeBruyn JM, Cregger MA, Willcox E, Trigiano R, Hadziabdic D. Signatures of prescribed fire in the microbial communities of Cornus florida are largely undetectable five months post-fire. PeerJ. 2023 Aug 24;11:e15822.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Reyes AM, Smith ZC, Onufrak AJ, Pietsch GM, Ony M, Odoi ME, Khodaei S, Smallwood CJ, Ginzel M, Klingeman WE, Hadziabdic D. First Report of Bot Canker (Diplodia corticola) in Pin Oak (Quercus palustris) in Tennessee. Plant Disease. 2023 Dec 1;107(12):4019.
- Type:
Theses/Dissertations
Status:
Accepted
Year Published:
2023
Citation:
Characterization of Plant-Microbial Interactions in the Thousand Cankers Disease Complex using Amplicon Sequencing, Transcriptomics, and Genomics
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2023
Citation:
Onufrak A.J., Pietsch G., Givens S., Klingeman D., Klingeman W., Cregger M., Walters K., Shekoofa S., Hadziabdic D. (2023) A not so dry subject: The responses of microbial communities associated with Geosmithia morbida infected Juglans nigra trees to drought stress. American Phytopathological Society Conference, Denver, CO (August 2023). Oral Presentation.
|
Progress 01/01/22 to 12/31/22
Outputs
Target Audience:During this reporting period I have worked with the following target audiences: 1. Middle and high school students: I have mentored middle and high school students as part of the Virtual Science Club. As part of my mentoring, I helped students develop their science fair projects for the Southern Appalachian Science and Engineering Fair. I also mentored a high school senior. Teaching her the basics of microbiology and statistical analyses for herSouthern Appalachian Science and Engineering Fair project. 2. Undergraduate Students: I participated as a mentor for the Bioinformatics, Genetics, and Genomics REEU. As part of my mentoring duties, I taught 6 female undergraduate students how to analyze amplicon sequencing data formicrobial community analyses. Furthermore, I worked with two independent study students on a project to characterize the effects of juglone on entomopathogens. These students presented their data at the University of Tennessee undergraduate research symposium. 3. Graduate Students: As part of my responsibilites as Hadziabdic lab manager, I havementored3 graduate students. As part of my mentoring, I provided peer edits on manuscripts and grant proposals and also aided in planning and carrying out experiments. 4. Visiting Scholars: I mentored two visiting scholars, one from Iran and another from Honduras. As part of my mentoring duties, I taught both visiting scholars how to measure gravimetric transpiration, stomatal conductance, and collect plant tissues for RNA and DNA extraction. Additionally, I taught the Honduran visiting scholar how to complete Koch's postulates. Changes/Problems:In regards to Training/Career Development Goal 3: Develop teaching skills by teaching high school students the basics of plant pathology as part of the Pre-College Upward Bound Program and teaching undergraduate students the basics of microbiome data analysis as part of the USDA BiGG Data REEU. Additionally, I will develop and teach a 3 credit graduate level course on microbiome data analysis and complete the UT Graduate School's Best Practices in Teaching. I was unable to teach the 3-credit graudate level course on microbiome data analysis in the Fall of 2022 due to changes in UT policy. However, I did develop and teach the course in Fall of 2021 and complete UT Graduate School's Best Practices in Teaching prior to the start of the funding. What opportunities for training and professional development has the project provided?Training/Career Development: 1.In summer 2022, I conducted a greenhouse experiment that addresses research objective1(QuantifyG. morbidavirulence in drought-stressed trees and identify responses of theJ. nigraphytobiome to drought) and objective 2 (Identify responses of host/pathogen interactions to drought inG. morbidainfectedJ. nigratrees). As part of this research I learned how to measure plant physiological variables related to drought stress and improved my project management and troubleshooting skills.Additionally, I established a collaboration with Scott Gula, a PhD student at Purdue University who will characterize the chemical profile of eastern black walnut wood exposed to drought using bark and phloem samples collected during this study. 2. I participated as a mentor in the virtual science club for the spring 2022 and fall 2022. As part of this process, I have mentored middle school and high school students, helping them develop ideas for their science fair project. Two of these students received Certificates of Excellence at the Southern Appalachian Science and Engineering Fair. 3. In Summer 2022, I taught a visiting scholar and undergraduate student from Honduras, the basics of plant pathology and how. to conduct Koch's postulates. As a result of my teaching efforts, they completed Koch's postulates, which will result in a first report publication. Additionally, I mentored 6 female undergraduate students as part of the USDA BiGG Data REEU. I taught these students how to process amplicon sequences and perform statistical analyses in R. How have the results been disseminated to communities of interest?As part of my training/development goals, I presented results ofmy dissertation research at the following conferences: The American Phytopathological Society Plant Health 2022 annual conference The Role of Microbiones in Forest Disease, Regeneration, and Biodiversity Webinar, hosted by the American Phytopathological Society I also was a co-author on a book chapter, and an extension document. What do you plan to do during the next reporting period to accomplish the goals?Training/Career Development 1. In Spring 2023, I will complete RNA and DNA extractions and analyze the resulting data using a variety of bioinformatics tools to increase my experience analyzing RNASeq and amplicon sequencing data. 2. In Spring 2023 and Fall 2023, I will continue to serve as a mentor as part of the virtual science club, helping students prepare projects for the Southern Appalachian Science and Engineering Fair. 3. In Summer 2023, I will serve as the lead mentor for the USDA BiGG Data REEU, teaching another cohort of undergraduate students how to process and analyze microbiome data. 4. In 2023, I anticipate submitting4 of my dissertation chapters for publication in peer-reviewed journalsand presenting the results of my original research projectat the following two conferences: Plant Health 2023, American Phytopathological Society, Denver, Colorado, August 12-16, 2023 ICPP 2023, Internation Congress of Plant Pathology, Lyon, France, August 20-25, 2023
Impacts
What was accomplished under these goals?
Research Project: 1. In summer 2022, we conducted a greenhouse experiment to characterize the effects of drought on the virulence ofGeosmithia morbidain drought-stressed black walnut trees. We innouclated 78 trees with G. morbidain 4 different positions along each stem. The study lasted for a total of 20 days with samples collected at day 3, day 8, day 14, day 15, and day 20 post-inoculation. A total of 24 trees were maintained as well-watered controls and40 were used as drought stressed.An addition24 trees were used as a rewatered treatment, which were exposed to drought until day 14 post-inoculation and then rewatered to test the effects of supplemental irrigation on the virulence and host-pathogen interaction. We determined that cankers were larger in drought stressed trees at days 14, 15, and 20 post-inoculation. Additionally, we determined that gravimetic transpiration rates and leaf water potential were generally lower drought stressed trees compared to well-watered controls. 2. We are currently in the process of extracting RNA and DNA from phloem tissues for characterization of host and pathogen transcriptomes and host microbial communities, respectively. Current extraction protocol haveyielded high quality RNA and DNA suitable for sequencing.
Publications
- Type:
Book Chapters
Status:
Published
Year Published:
2022
Citation:
Hadziabdic D., Onufrak A.J., Gazis R. (2022) Fungi in forested ecosystems, in Mycoagroecology: integrating fungi into agroecological ecosystems, ed. Elizabeth Gall and Noure Benkeblia. Boca Raton, US: CRC Press
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2022
Citation:
Onufrak A.J., Pietsch G., Klingeman W., Cregger M., DeBruyn J., Ginzel M., and Hadziabdic D. (2022) Peeling back the bark to characterize the responses of phloem-associated microbial communities in Geosmithia morbida-infected Juglans nigra trees. The American Phytopathological Society Plant Health, Pittsburgh, PA, August 6th 10th, 2022, Poster Presentation.
- Type:
Other
Status:
Published
Year Published:
2022
Citation:
Onufrak A.J., Klingeman W., Hadziabdic D. (2022) Identification and management recommendations of common fungal diseases of eastern Black Walnut, University of Tennessee Extension, https://extension.tennessee.edu/publications/Documents/W1101.pdf.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2022
Citation:
Onufrak A.J., Pietsch G., Klingeman D., Klingeman W., Cregger M., DeBruyn J., Ginzel M., and Hadziabdic D. (2022) Responses of Juglans nigra microbiome to Geosmithia morbida infection and candidate Thousand Cankers Disease Management Strategies. The Role of Microbiomes in. Forest Disease, Regeneration, and Biodiversity Webinar, The American Phytopathological Society, October 11th, 2022, Oral Presentation.
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