Source: VIRGINIA STATE UNIVERSITY submitted to NRP
MODIFICATION OF HEMP SEED PROTEIN FOR IMPROVED FUNCTIONALITIES USING HIGH HYDROSTATIC PRESSURE PROCESSING
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1027986
Grant No.
2022-67018-36599
Cumulative Award Amt.
$274,500.00
Proposal No.
2021-09353
Multistate No.
(N/A)
Project Start Date
Jan 1, 2022
Project End Date
Dec 31, 2025
Grant Year
2022
Program Code
[A1364]- Novel Foods and Innovative Manufacturing Technologies
Recipient Organization
VIRGINIA STATE UNIVERSITY
(N/A)
PETERSBURG,VA 23803
Performing Department
Dept Of Agricultural Research
Non Technical Summary
Consumers and food manufacturers are increasingly interested in plant-based proteins for their sustainability, and other economic, and health benefits. Soy is a commonly used protein source for product development, but increasing demand for plant proteins is driving science-based exploration for new and non-traditional sources. Hemp protein, a by-product of oil extraction from hemp seed, has excellent nutritional qualities, making it a promising novel and alternative plant protein. Although hemp protein is highly nutritious, its compact structure limits its functionalities and usefulness for many food applications. High-pressure processing (HPP) is an effective method for modifying protein structure and enhancing functional properties.The objective of this seed grant is to demonstrate the capacity of HPP treatment to alter hemp protein structure and improve functional properties important in food matrices. We will utilize HPP to induce structural changes in hemp protein isolates, evaluate their structural, physicochemical, and functional properties, and conduct a preliminary study to formulate HPP-treated hemp protein isolate-based yogurt.Research described herein will advance our knowledge and understanding of this novel protein source and methods for enhancing its functionality. It addresses USDA-AFRI A1364 Program Priority Area of 'Improve our knowledge and understanding of the chemical, physical, and biological properties of novel foods and food ingredients.' It also lays a foundation for our long-term goal to develop high-functionality hemp protein for use in high-quality plant-based food products. Proposed research and future directions are expected to benefit stakeholders by increasing value and demand for hemp protein and revenue to producers and manufacturers across the supply chain.
Animal Health Component
40%
Research Effort Categories
Basic
50%
Applied
40%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
5021730200050%
5011730202050%
Goals / Objectives
Our long-term goal is to develop high-functionality hemp protein for use in high quality plant-based food products. The objective of this seed grant is to demonstrate the capacity of HPP treatment to alter hemp protein structure and improve functional properties important in food matrices.
Project Methods
Methods to conduct this research include:Task 1. Utilize HPP to induce structural changes in hemp protein isolates. Hemp protein will first be isolatedusing alkaline extraction/isoelectric precipitation method andthen freeze-dried prior to further processing and characterization. After isolation, HPP treatments will be conducted using a laboratory-scale high-hydrostatic pressure system.A central composite experimental design will employ 3 variables with 5 levels per variable.Independent variables will include protein concentration, pressure level, and holding time.Samples will be freeze-dried after treatment pending further analysis.Task 2. Evaluate structural, physicochemical, and functional properties of HPP-treated compared to untreated hemp protein isolates. A series of properties, including physicochemical, structural and functional propertiesof untreated and HPP-treated hemp protein will be evaluated.Physicochemical propertiesincludeproximate composition, amino acid profiles, in-vitro protein digestibility, and color. Furthermore, protein subunits, secondary structure, and functional groups important for structural stability, unfolding and surface properties of hemp protein isolates will be measured before and after HPP treatment to fully understand HPP-induced structural and conformation changes. Finally, someimportant functional properties, including protein solubility, emulsification, water and oil absorption, foaming, gelation and rheological behavior will be determined.Task 3. Conduct a preliminary study to formulate hemp protein-based yogurt analog and compare its properties to those of commercial yogurt. Aproof-of-concept study using yogurt as a model food system will be conducted. Hemp protein isolate-based yogurt analogs will beformulated. Rheological and sensory properties will be evaluated whencomparing them with commercial yogurt.

Progress 01/01/24 to 12/31/24

Outputs
Target Audience:Hemp seed protein, an underexploited resource, is nutritious and highly digestible, but information on its structural and functional properties is needed by food manufacturers, processors, and fumulators to expand utilization. Such information may be of value also for agronomists and plant breeders Changes/Problems:We discovered that freeze-drying the processed hemp seed protein is a signifcant limitation in sample preparation. Each sample takes days to completely freeze-dry due to large volume (about 500 mL). Special handling of the samples is necessary to avoid melting. Only a few preparations can be freeze-dried. simultaneously to avoid overloading the freezedrier system. What opportunities for training and professional development has the project provided?• Student training: 1 undergraduate student assisted in conducting experiments • One part-time research technician was hired How have the results been disseminated to communities of interest?One paper was published in a peer-reviewed scientific journal. Two presentation were made by student research assistants at the 2022 Association of 1890 Research Directors biennial conference What do you plan to do during the next reporting period to accomplish the goals?• High hydrostatic pressure (HHP) will be used to treat hemp heart protein isolate. Physicochemical, functional, and structural properties of treated protein will be evaluated relative to untreated isolate to determine the effectiveness of HHP for modification of protein characteristics to potentially expand the utility of hemp heart protein for food applications.

Impacts
What was accomplished under these goals? Contact was made with a commercial producer of hemp heart protein and hemp protein preparations were obtained fromthe same. • Physicochemical, functional, and structural properties of commercially available hemp heart protein, an underexploited "novel" protein resource, were made in comparison to other commercially available plant and animal proteins to establish baseline characteristics. Physicochemical properties included color, proximate and amino acid compositions, and molecular weight profiles. Functional properties included water and oil holding capacities, emulsification activity and stability, foaming capacity, and solubility. Secondary protein structure was evaluated from deconvolved Amide I band spectra. Tertiary structure was evaluated using surface hydrophobicity and free-sulfhydryl content. • One paper was published and two student presentations at a national convention were made.

Publications

  • Type: Other Journal Articles Status: Published Year Published: 2022 Citation: Physicochemical, Structural, and Functional Properties of Hemp Protein vs Several Commercially Available Plant and Animal Proteins: A Comparative Study. Y. Xu and 8 coauthors. ACS Food Science & Technology 2022 2 (10), 1672-1680. DOI: 10.1021/acsfoodscitech.2c00250
  • Type: Conference Papers and Presentations Status: Other Year Published: 2022 Citation: The effect of protein contents on physicochemical, microstructural, functional properties of hemp heart protein. A. Jacobs, G. DeSousa, J. Britland, E. Sismour, Z. Abraha-Eyob, and Y. Xu. 20th Biennial Research Symposium, April 2-5, 2022. Atlanta, Georgia
  • Type: Conference Papers and Presentations Status: Other Year Published: 2022 Citation: Physicochemical, functional properties and in-vitro digestibility of hemp protein compared with pea and egg protein. A. Sellers, A. Cunningham, J. Britland, E. Sismour, Z. Abraha-Eyob, and Y. Xu. 20th Biennial Research Symposium, April 2- 5, 2022. Atlanta, Georgia.


Progress 01/01/23 to 12/31/23

Outputs
Target Audience:Hemp seed protein, an underexploited resource, is nutritious and highly digestible, but information on its structural and functional properties is needed by food manufacturers, processors, and fumulators to expand utilization. Such information may be of value also for agronomists and plant breeders Changes/Problems:We discovered that freeze-drying the processed hemp seed protein is a signifcant limitation in sample preparation. Each sample takes days to completely freeze-dry due to large volume (about 500 mL). Special handling of the samples is necessary to avoid melting. Only a few preparations can be freeze-dried. simultaneously to avoid overloading the freeze-drier system. What opportunities for training and professional development has the project provided?• Student training: 1undergraduate studentassisted in conducting experiments • One part-time research technician was hired How have the results been disseminated to communities of interest?One paper was published in a peer-reviewed scientific journal. Two presentation were made by student research assistants at the 2022 Association of 1890 Research Directors biennial conference What do you plan to do during the next reporting period to accomplish the goals?• High hydrostatic pressure (HHP) will be used to treat hemp heart protein isolate. Physicochemical, functional, and structural properties of treated protein will be evaluated relative to untreated isolate to determine the effectiveness of HHP for modification of protein characteristics to potentially expand the utility of hemp heart protein for food applications.

Impacts
What was accomplished under these goals? • Contact was made with a commercial producer of hemp heart protein and hemp protein preparations were obtained from the same. • Physicochemical, functional, and structural properties of commercially available hemp heart protein, an underexploited "novel" protein resource, were made in comparison to other commercially available plant and animal proteins to establish baseline characteristics. Physicochemical properties included color, proximate and amino acid compositions, and molecular weight profiles. Functional properties included water and oil holding capacities, emulsification activity and stability, foaming capacity, and solubility. Secondary protein structure was evaluated from deconvolved Amide I band spectra. Tertiary structure was evaluated using surface hydrophobicity and free-sulfhydryl content. • One paper was published and two student presentations at a national convention were made.

Publications

  • Type: Journal Articles Status: Published Year Published: 2022 Citation: Physicochemical, Structural, and Functional Properties of Hemp Protein vs Several Commercially Available Plant and Animal Proteins: A Comparative Study. Y. Xu and 8 coauthors. ACS Food Science & Technology 2022 2 (10), 1672-1680. DOI: 10.1021/acsfoodscitech.2c00250
  • Type: Conference Papers and Presentations Status: Other Year Published: 2022 Citation: The effect of protein contents on physicochemical, microstructural, functional properties of hemp heart protein. A. Jacobs, G. DeSousa, J. Britland, E. Sismour, Z. Abraha-Eyob, and Y. Xu. 20th Biennial Research Symposium, April 2-5, 2022. Atlanta, Georgia
  • Type: Conference Papers and Presentations Status: Other Year Published: 2022 Citation: Physicochemical, functional properties and in-vitro digestibility of hemp protein compared with pea and egg protein. A. Sellers, A. Cunningham, J. Britland, E. Sismour, Z. Abraha-Eyob, and Y. Xu. 20th Biennial Research Symposium, April 2- 5, 2022. Atlanta, Georgia.


Progress 01/01/22 to 12/31/22

Outputs
Target Audience: Hemp seed protein, an underexploited resource, is nutritious and highly digestible, but information on its structural and functional properties is needed by food manufacturers, processors, and fumulators to expand utilization. Such information may be of value also for agronomists and plant breeders. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Student training: 3 undergraduate students and 2 dietetic interns assisted in conducting experiments One part-time research technician was hired How have the results been disseminated to communities of interest? One paper was published in a peer-reviewed scientific journal. Two presentation were made by student research assistants at the 2022 Association of 1890 Research Directors biennial conference. What do you plan to do during the next reporting period to accomplish the goals? High hydrostatic pressure (HHP) will be used to treat hemp heart protein isolate. Physicochemical, functional, and structural properties of treated protein will be evaluated relative to untreated isolate to determine the effectiveness of HHP for modification of protein characteristics to potentially expand the utility of hemp heart protein for food applications.

Impacts
What was accomplished under these goals? Contact was made with a commercial producer of hemp heart protein and hemp protein preparations were obtained from the same. Physicochemical, functional, and structural properties of commercially available hemp heart protein, an underexploited "novel" protein resource, were made in comparison to other commercially available plant and animal proteins to establish baseline characteristics. Physicochemical properties included color, proximate and amino acid compositions, and molecular weight profiles. Functional properties included water and oil holding capacities, emulsification activity and stability, foaming capacity, and solubility. Secondary protein structure was evaluated from deconvolved Amide I band spectra. Tertiary structure was evaluated using surface hydrophobicity and free-sulfhydryl content. One paper was published and two student presentations at a national convention were made.

Publications

  • Type: Journal Articles Status: Published Year Published: 2022 Citation: " Physicochemical, Structural, and Functional Properties of Hemp Protein vs Several Commercially Available Plant and Animal Proteins: A Comparative Study. Y. Xu and 8 coauthors. ACS Food Science & Technology 2022 2 (10), 1672-1680. DOI: 10.1021/acsfoodscitech.2c00250
  • Type: Conference Papers and Presentations Status: Other Year Published: 2022 Citation: " Physicochemical, functional properties and in-vitro digestibility of hemp protein compared with pea and egg protein. A. Sellers, A. Cunningham, J. Britland, E. Sismour, Z. Abraha-Eyob, and Y. Xu. 20th Biennial Research Symposium, April 2-5, 2022. Atlanta, Georgia
  • Type: Conference Papers and Presentations Status: Other Year Published: 2022 Citation: " The effect of protein contents on physicochemical, microstructural, functional properties of hemp heart protein. A. Jacobs, G. DeSousa, J. Britland, E. Sismour, Z. Abraha-Eyob, and Y. Xu. 20th Biennial Research Symposium, April 2-5, 2022. Atlanta, Georgia. (2nd place award winner - Food Safety, Nutrition, Health and Health Disparities division)