Progress 05/15/24 to 05/14/25
Outputs
Target Audience:Industry Collaborators: During the reporting period, we worked with our industry collaborators, the Michigan Turkey Producers and Miller Poultry. We also interacted with the rural farmer community in West Alabama. Agricultural Producers: We presented our research at the 2024 Great Lakes Fruit, Vegetable, and Farm Market Expo & Michigan Greenhouse Growers Expo ("GLEXPO"), Dec 9-12, 2024. More than 3,000 people attended the GLEXPO, with close to 400 exhibitors. Survey data revealed that GLEXPO sessions reached a regional audience from 13 countries, 7 Canadian provinces, 41 US states and the District of Columbia, and 62 Michigan counties. Most attendees were fruit growers and farm marketers. Science Festival: On April 14, 2024, we presented our research at the MSU Science Festival. The Science Festival covers four weekends in April, and it is a free annual celebration of science fueled by curiosity, wonder, and discovery, which are essential to scientific inquiry. With events for the whole family to enjoy, the 2024 MSU Science Festival explored topics ranging from the ordinary to the extraordinary. From astronomy to music, chemistry to microbiology, scientists and educators across the stateshared the wonders of science, technology, engineering, art, and mathematics. Schoolchildren from K-12, parents, and grandparents attended the event. World Food Prize Michigan Youth Institute: The World Food Prize Michigan Youth Institute (WFPMYI) is a one-day event that allows high school students to present research and recommendations on solving key global challenges. On May 9, 2024, we hosted attendees of the WFPMYI in the lab and shared our research project. General public: We presented our research to students and parents attending the Distinguished Scholarship event in February 2024. Students: We trained undergraduate, graduate, and DVM students during the reporting period. Scientific Communities: We presented our results at the following conferences, representing various scientific groups: Institute of Biological Engineers (IBE) meeting in Atlanta, GA, June 13-15, 2024 American Society of Agricultural and Biological Engineers (ASABE) meeting in Anaheim, CA, July 28-31, 2024 Multistate committee NC-1194 (Nanotechnology and Biosensors) in Chicago, IL, July 17-18, 2024 Annual Biomedical Research Conferencefor Minoritized Scientists (ABRCMS), Pittsburgh, Pennsylvania, November 13-16, 2024 NDiSTEM Conference, SACNAS, Phoenix, Arizona, October 31-November 2, 2024 University Undergraduate Research and Arts Forum (UURAF), Michigan State University, April 12, 2024 Changes/Problems: The tendency of Campylobacter jejuni to become viable but not culturable made it challenging to validate the technology in real samples. To validate the technology, we will explore alternative and complementary means of colony count. A complex sample matrix diminishes the efficiency of magnetic enrichment. We will explore new techniques to minimize the matrix effect as we validate the nano-biosensor in on-site settings. We will explore and design different probes for the multi-array biosensor platform. Commercially available DNA extraction kits are challenging to operate in the field or in on-site conditions. We will explore field-operable and simple DNA extraction procedures. What opportunities for training and professional development has the project provided?Training opportunities on this project were provided to: • Ten graduate students (3 MSU, 7 TU) • Six undergraduate research assistants (4 during the semesters, 2 in summer, all at MSU) Three of our students received four awards, demonstrating the high quality of students in this project. Anthony James Franco, 2024. Best presentation award, ASABE 2024, Anaheim, CA, July 28-31, 2024 Anthony James Franco, 2024. Presentation Excellence Award (top 15% of student presentations) ASABE 2024, Anaheim, CA, July 28-31, 2024 Kaily Kao, 2024. Best poster award, ASABE 2024, Anaheim, CA, July 28-31, 2024 Leah Wilson, 1st place poster award, UURAF 2024, Michigan State University, East Lansing, MI, April 12, 2024 How have the results been disseminated to communities of interest?Results from this project were disseminated through peer-reviewed journal publications and presentations at technical conferences. For journal publications, see the list of papers. For presentations, see the list below. Invited Talks: Alocilja E. 2024. Nano-Biosensor for Rapid AMR Characterization and Detection, Biosensors Journal Webinar, April 11, 2024, invited speaker Alocilja EC. 2024. Sustainable Health On Your Hand with SMART Biosensor, Great Lakes Expo, DeVos Center, Grand Rapids, MI, December 11, 2024 Alocilja E. 2024. Monitoring Antimicrobial Resistance in One Health Using Nano-Biosensors, Mekong One Health Conference or MOHIP symposium "Strengthening Health Security of the Lower Mekong Subregion," May 15, 2024, Virtual Alocilja E. 2024. SMART Biosensors for One Health Monitoring and Surveillance, Mekong One Health Innovation Program (MOHIP) Virtual Webinar, January 18, 2024 Alocilja E. 2024. Short Course on SMART Biosensors for One Health Monitoring and Surveillance, Mekong One Health Innovation Program (MOHIP) Short Course, February 29, 2024 Alocilja E. 2024. One Health On Your Hand with SMART Biosensor, Schlumberger Foundation, Virtual, December 5, 2024 Alocilja E. 2024. Nanotechnology-Enabled Biosensors for Rapid Detection of Infectious and Drug-Resistant Diseases, Silliman University, Philippines, May 14, 2024 Alocilja E. 2024. Nanotechnology-Enabled Biosensors for Rapid Detection of Infectious Diseases in Humans, Animals, Plants, and the Environment, Cavite State University, Philippines, May 22, 2024 Alocilja E. 2024. Broadening Participation in STEM, Best Practices and MSU partners, Invited Panelist, Kellogg Center, MSU, Nov. 12, 2024 GARD-Innovation Challenge, Mandela Fellows, MSU, July 15, 2024 Presentations at Professional Conferences: Gregory D, Cayen J, and Alocilja EC. 2024. Assessing Antibiotic Resistance in E. coli and K. pneumoniae Using Zeta Potential, 2024 ASABE Annual Meeting, Anaheim, CA, July 28-31, 2024 Heinecke K, Franco AJ, and Alocilja EC. 2024. Simultaneous Isolation of Foodborne Pathogens Using Carbohydrate-Coated Magnetic Nanoparticles, 2024 ASABE Annual Meeting, Anaheim, CA, July 28-31, 2024 Wilson L, Franco AJ, and Alocilja EC. 2024. Investigation on the Concentration of Salmonella using Glycan-Coated Magnetic Nanoparticles, 2024 ASABE Annual Meeting, Anaheim, CA, July 28-31, 2024 Franco AJ and Alocilja EC. 2024. Co-Detection of Salmonella and E. coli using a label-free plasmonic genosensor, 2024 ASABE Annual Meeting, Anaheim, CA, July 28-31, 2024 Kao K and Alocilja EC. 2024. Rapid Detection of Antimicrobial Resistant Genes in E. coli Using a Plasmonic Biosensor, 2024 ASABE Annual Meeting, Anaheim, CA, July 28-31, 2024 Caliskan-Aydogan O, Sharief SA, and Alocilja EC. 2024. Nanoparticles for Detection of Salmonella from Chicken Meat, American Society for Microbiology, Atlanta, GA, June 13-15, 2024 Kao K, Caliskan-Aydogan O, and Alocilja EC. 2024. Multi-Array Biosensor for Onsite Detection of Antimicrobial Resistant Genes for One Health, Institute of Biological Engineers Annual Meeting, Atlanta, GA, Sept. 13-15, 2024 Franco AJ and Alocilja EC. 2024. Applying Image Analysis for Rapid and Practical Detection of Foodborne Pathogens, Institute of Biological Engineers Annual Meeting, Atlanta, GA, Sept. 13-15, 2024 Alocilja EC, 2024. Nano-Biosensor for Point-of-Use Detection of Foodborne and AMR Pathogens, NC-1194 Annual Meeting, Chicago, IL, July 17-18, 2024 Alocilja EC et al, 2024. PARTNERSHIP: Development of a Nanoparticle-enabled Enrichment and DNA-Biosensing for Onsite Monitoring of Multiple Foodborne Pathogens in Large Samples: Progress Report 2023-2024, CA, July 13, 2024 Suministrado K, Amalin D, and Alocilja EC. 2024. Early Detection in Potential Insect Vectors in Ralstonia solanacearum, Kyoto International Conference Center (ICC Kyoto), Japan, August 25-30, 2024 Yrad FM and Alocilja EC. 2024. Functionalization of Dextrin-capped Gold Nanoparticles with Oligodeoxynucleotides, World Nanotechnology Conference, Singapore, March 18-20, 2024 Villatoro K, Franco AJ, and Alocilja EC. 2024. The adsorption profiles of different Salmonella serotypes on glycan-coated magnetic nanoparticles, SACNAS, Phoenix, Arizona October 31 - November 2, 2024 Villatoro K, Franco AJ, and Alocilja Villatoro K, Franco AJ, and Alocilja EC. 2024. The adsorption of differentSalmonellaserotypes on glycan-coated magnetic nanoparticles, Annual Biomedical Research Conferencefor Minoritized Scientists (ABRCMS), Pittsburgh, Pennsylvania, November 13-16, 2024 Heinecke K, Franco AJ, and Alocilja EC. 2024. Simultaneous Isolation of Foodborne Pathogens Using Carbohydrate-Coated Magnetic Nanoparticles, University Undergraduate Research and Arts Forum (UURAF), Michigan State University, April 12, 2024 Gregory D, Cayen J, and Alocilja EC. 2024. Assessing Antibiotic Resistance in E. coli, K. pneumoniae, and E. cloacae Using Zeta Potential, University Undergraduate Research and Arts Forum (UURAF), Michigan State University, April 12, 2024 Wilson L, Franco AJ, and Alocilja EC. 2024. Investigation on the Concentration of Salmonella using Glycan-Coated Magnetic Nanoparticles, University Undergraduate Research and Arts Forum (UURAF), Michigan State University, April 12, 2024; 1st place poster winner Kao K and Alocilja EC. 2024. Rapid Detection of Carbapenem-Resistant Genes in E. coli Using Plasmonic Biosensor, University Undergraduate Research and Arts Forum (UURAF), Michigan State University, April 12, 2024 Franco AJ, Heinecke K, and Alocilja EC. 2024. Adsorption kinetics of bacterial isolation using glycan-coated magnetic nanoparticles, 2024 Engineering Graduate Research Symposium, May 2, 2024 Narwal S and Alocilja EC. 2024. Automated data analysis for enhanced diagnostic accuracy: pathogen extraction using magnetic nanoparticles and DNA detection using gold nanoparticles, Mid-SURE, MSU, July 23-24, 2024 Villatoro, K, Franco AJ, and Alocilja EC. 2024. The adsorption of differentSalmonellaserotypes on glycan-coated magnetic nanoparticles, Mid-SURE, MSU, July 23-24, 2024 Villatoro, K, Franco AJ, and Alocilja EC. 2024. The adsorption kinetics of differentSalmonellaserotypes on glycan-coated magnetic nanoparticles, Alliances for Graduate Education and the ProfessoriateStudent Success Conference(MSU AGEP SSC), Michigan State University,October 4, 2024 Rejoice Nyarku, Kierney Burks, Kingsley Bentum, Rawah Faraj, Alocilja Evangelyn, Woubit Abebe. 2024. Integrating GenoPATHX assay and Nano Biosensor Technologies for Comprehensive Analysis of Salmonella Serovar Dynamics in Poultry Products, Tuskegee University. What do you plan to do during the next reporting period to accomplish the goals?Plans for next year: Continue to improve the smartphone application for image display and analysis for the biosensing assay. Validate the magnetic enrichment in collaborators' farms & processing plants - extraction onsite. Validate the SMART biosensor in collaborators' farms & processing plants - biosensor tested onsite. Validate and compare the biosensor with Neogen's DNA kit. Generate data for temporal dynamics analysis. Calculate costs associated with the biosensor production and use in farms and processing plants. Calculate the benefits associated with the biosensor technology compared with existing technologies. Assess the initial technology readiness of the biosensor. Meet with collaborators and other stakeholders to assess the significance of results and technology readiness. We plan to request a no-cost extension to fully accomplish objective 3 and conduct a technology readiness assessment with our industry collaborators.
Impacts
What was accomplished under these goals?
Year 3 Project Milestones and Accomplishments Objective 1: Optimize the Site-Enriched Multi-Array Reporting Biosensing Technology (SMART) for rapid detection of Salmonella and Campylobacter in large samples. Proposed Activity: Validate enrichment in samples from poultry farm (manure) and slaughterhouse (scald water, broiler/turkey) - samples sent to lab. Accomplishments: The magnetic enrichment was validated in 50-100 mL of carcass rinsate, resuspended swabs, and 25 grams of ground meat. The concentration factor (CF), the magnetic nanoparticles' (MNP) ability to isolate and concentrate the bacteria from a large volume to a small volume, like 1 mL, ranged from 1.03 for rinsate to 2.54 for ground turkey and chicken meat. These results showed that magnetic enrichment could isolate bacterial cells, resulting in a rapid cleanup for any downstream process. The magnetic enrichment was also validated in poultry manure. The CF value was slightly below 1.0, meaning some bacterial cells were not visible on the plate. This is potentially due to the complexity of the sample matrix, such as pH, particle size, makeup of manure solids (fixed and volatile solids, suspended solids, and dissolved solids), nutrient content (organic and inorganic), and other factors. Nevertheless, results showed that magnetic enrichment isolated the bacteria from the complex matrix, preparing cleaned-up cells for any downstream process, such as biosensing detection. Proposed Activity: Validate biosensor in samples from poultry farm (manure) and slaughterhouse (scald water, broiler/turkey) - samples sent to lab. Accomplishments: Oligonucleotide probes for detecting three Salmonella enterica genes and one probe for detecting one Campylobacter jejuni gene were designed and validated in the nano-biosensor. The sensitivity of both the Salmonella and Campylobacter nano-biosensors was determined at 1.25 ng/µL of dsDNA. The nano-biosensor was selective against E. coli, S. aureus, and L. monocytogenes. The nano-biosensor detected Salmonella in carcass swabs, rinsate, and ground meat. The results agreed with culture- and PCR-based detection methods, with a small degree of false positive (2/16) and false negative (1/16) results relative to culture-based detection. All the above accomplishments demonstrate that the proposed activities in objective 1 have been accomplished. Objective 2: Optimize cellphone-based data capture, analysis, and reporting of the biosensor signal output. Proposed Activity: Optimize app for image/data capture, analysis, and display. Accomplishments: The smartphone application (app) image analysis agreed with the spectrometric measurements in sample analysis. Signal correlation between the image analysis (R/G) and spectrometric measurements (A520/A620) showed that the smartphone app can be an alternative to spectrometric measurements. However, the smartphone app's sensitivity diminishes near the detection limit of spectrometric measurements. The smartphone app was fine-tuned to improve user friendliness and configured to interface with a central database in the future. Objective 3: Validate the cellphone-enabled SMART biosensor for simultaneous detection of Salmonella spp. and Campylobacter spp. in selected poultry farms and slaughter facilities. Accomplishments: A simple DNA extraction procedure used directly on magnetically enriched samples was developed to eliminate the need for expensive DNA extraction kits. We coordinated with collaborators to plan for field testing the nano-biosensor. We continued to validate and fine-tune the biosensor samples sent to the lab in preparation for the field testing and temporal data gathering.
Publications
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Caliskan-Aydogan O, Zaborney Kline CE, Alocilja EC. 2024. Adhesion Capacity of Carbapenem-Resistant E. coli with Magnetic Nanoparticles, Nanomaterials, Nanomaterials 2024, 14(24), 2010
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Mendoza DJ, Atienza-Parcon MTJA, Alocilja EC, and Fernando LM. 2024. Magnetic nanoparticle-mediated extraction and electrochemical detection of E. coli O157:H7 Genomic DNA, Analytical Letters, 116
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Alocilja, Evangelyn C. 2024. Re-engineering Agricultural Innovation in Southeast Asia (RAISE-Asia). Asian Journal of Agriculture and Development 21(20th Anniversary Issue): 113-128
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Ghazy A, Nyarku R, Faraj R, Bentum K, Woube Y, Williams M, Alocilja E, and Abebe W. 2024. Gold Nanoparticle-based Plasmonic Detection of Escherichia coli, Salmonella enterica, Campylobacter jejuni, and Listeria monocytogenes from bovine fecal Samples, Microorganisms, 12, 1069
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Caliskan-Aydogan O, Zaborney Kline C, and Alocilja EC. 2024. Cell Morphology as Biomarker of Carbapenem Exposure, Journal of Antibiotics 77, pages 600611
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Caliskan-Aydogan O and Alocilja EC. 2024. A Parallel biosensor platform for the detection of carbapenemase-producing E. coli in spiked food and water samples, Food Control, Vol. 163, 110485.
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Progress 05/15/23 to 05/14/24
Outputs
Target Audience:Industry collaborators: During the reporting period, we worked with our industry collaborators, the Michigan Turkey Producers and Miller Poultry. We collected samples from their processing plants, the Doherty Turkey Farm, and a Miller Poultry farm contractor in Indiana. We also contacted turkey and poultry farms in Alabama to validate the testing of Salmonella serovar. General public: We presented our research results to the public at the MSU Science Festival in April 2024, which schoolchildren from K-12 and parents from around the state of Michigan attended. We interacted with the rural farmer community in West Alabama. Students: We trained undergraduate, DVM, and graduate students and visiting scholars during the reporting period. Professional societies: We presented our results at the following conferences: Institute of Biological Engineers (IBE) in Iowa American Society of Agricultural and Biological Engineers (ASABE) in Nebraska International Association for Food Protection (IAFP) in Canada Phi Zeta Research Symposium in Alabama Multistate committee NC-1194 (Nanotechnology and Biosensors) in Florida Comments from our stakeholders, 2023-2024: "We continue to be very excited about the rapid detection of Salmonella with the technology you are developing for our industry. We believe the technology will be very useful when monitoring the cleanliness of finishing barns after clean-out, between flocks. It is very important to our growers to have our barns as clean as possible when moving flocks from the brooders to the finishing barns, the use of the rapid technology can help the farm managers assess the cleanliness of the barns prior to movement. The 4-hour result time allows our farms to have time to re-clean and re-test areas of concern, allowing our birds the healthiest conditions possible. We are considering using the technology as a verification tool for our load-out crews. While we know that Salmonella resides in the gut of our birds, we also believe they will not shed the Salmonella unless we create an environment that causes them stress. If a barn tests negative prior to load-out, and positive after load-out, the data can be used for continuous improvement to better understand the things that cause our birds stress." Tina Conklin, Michigan Turkey "Continuous improvement in food safety in the area of pathogen control is of utmost importance. Timeliness, accuracy, and valid measurements of our processes are critical for quick and accurate assessments and decision making. Pathogens are grown and introduced to live poultry at the farm. It is beneficial to have quick and accurate measurement techniques on the farm to allow for faster intervention to greatly reduce the likelihood and the level of pathogens from reaching final product destined for consumers." Roger Stearns, Miller Poultry Changes/Problems:Acquiring and maintaining Campylobacter cultures have been challenging due to the specific atmosphere requirement and the preparation of the special media, which requires different cocktails of supplements. These challenges made the in vitro assays difficult. After experiencing these challenges, we have since adjusted our approach and considered the organism's special growing conditions. What opportunities for training and professional development has the project provided?Training opportunities on this project were provided to: Nine graduate students (3 MSU, 6 TU) Seven undergraduate research assistants (4 during the semesters, 3 in summer, all at MSU) One visiting scholar (1 MSU) Two professional DVM students (2 TU) Two of our students received awards, demonstrating the high quality of students in this project. Anthony James Franco, 2023. Best Poster Award, 2023 Engineering Graduate Research Symposium, Michigan State University, East Lansing, MI 48824, Aug. 22, 2023 Anthony James Franco, 2023. First Place winner, Data and Research Translation Award, 2023 Engineering Graduate Research Symposium, Michigan State University, East Lansing, MI 48824, Aug. 22, 2023 Devyn Hill, 2023. AGEP Conference, 2nd Place Oral Presentation for undergraduates, Michigan State University, East Lansing, MI 48824 How have the results been disseminated to communities of interest?Results from this project were disseminated through peer-reviewed journal publications and presentations at technical conferences. For journal publications, see the list of papers. For presentations, see the list below. Presentations at Professional Conferences: Franco AJ, Conklin T, Williams Z, Schweihofer J, Abebe W, and Alocilja EC, 2023. Rapid Salmonella Detection Using Nanoparticles: A Promising Technology for Safer Poultry Products, Poultry Summit, Atlanta, Georgia, Nov. 7-9, 2023 NC-1194 Committee meeting, Gainsville, Florida, Aug. 7-8, 2023 Franco AJ, Mayaka R, Abebe W, and Alocilja EC. 2023. Genomic Detection of Salmonella in Poultry Samples Using an End-to-End Nano-Biosensor Platform, International Association for Food Protection Annual Meeting 2023, Toronto, Canada, July 16-19, 2023 Caliskan-Aydogan, O., Sharief, S. A., & Alocilja, E. C. Nanoparticles for multiplex genomic detection of Carbapenem-Resistant E. coli in Foods, International Association for Food Protection (IAFP) International Meeting, Toronto, Ontario, Canada, July 16-19, 2023. Franco AJ, Conklin T, Schweihofer J, Williams Z, Alocilja EC. 2023. End-to-End Nano-Biosensor Platform for the Genomic Detection of Salmonella in Poultry Farm Samples, ASABE International Meeting, Omaha, NE, July 9-12, 2023 Mayaka R and Alocilja EC. 2023. A nano biosensor for the rapid detection of BlaNDM-1 Gene, ASABE International Meeting, Omaha, NE, July 9-12, 2023 Sharief S, Caliskan-Aydogan O, and Alocilja EC. 2023. PCR-less detection of E. coli from fresh produce using carbohydrate-coated nanoparticles, ASABE International Meeting, Omaha, NE, July 9-12, 2023 Caliskan-Aydogan O and Alocilja EC. 2023. DNA-Based Plasmonic Biosensor for KPC-producing Carbapenem-Resistant Bacteria, ASABE International Meeting, Omaha, NE, July 9-12, 2023 Alocilja, EC and Caliskan-Aydogan 2023. Nano-Biosensor for Genome-To-Phenome Profiling of AMR (G2PAR), Institute of Biological Engineers, Ames, Iowa, April 13-15, 2023 Franco AJ and Alocilja, EC 2023. Magnetically-Activated Cell Enrichment (MACE) for Improved Salmonella Detection, Institute of Biological Engineers, Ames, Iowa, April 13-15, 2023 Kierney Burks, Phi Zeta Research Symposium, College of Veterinary Medicine, Tuskegee University, September 2023 Presentations at MSU: Kao K, Cayen J, and Alocilja EC. 2023. Testing for Virulence Genes in E. coli O157 Using DNA Biosensor, University Undergraduate Research and Arts Forum (UURAF), Michigan State University, April 14, 2023 Heinecke K, Franco AJ, Alocilja EC, 2023. Magnetic Nanoparticle-Aided Rapid Screening of Microbial Contamination, University Undergraduate Research and Arts Forum (UURAF), Michigan State University, April 14, 2023 Zaborney Kline C and Alocilja EC, 2023. Microscopic Imaging of MNP-Bound Bacteria in Food Matrices, University Undergraduate Research and Arts Forum (UURAF), Michigan State University, April 14, 2023 Bieszke L, Franco AJ, and Alocilja EC. 2023. Extraction and Concentration of Salmonella from Poultry Plant Samples Using Magnetic Nanoparticles, University Undergraduate Research and Arts Forum (UURAF), Michigan State University, April 14, 2023 Wang P, Franco J, and Alocilja EC. 2023. Mid-SURE, Mid-SURE, Michigan State University East Lansing, MI. June 20, 2023 Amponsah E and Alocilja EC. 2023. Mid-SURE, Mid-SURE, Michigan State University East Lansing, MI. June 20, 2023 Hill D, Franco J, and Alocilja EC. 2023. Magnetic Nanoparticles (MNPs) for the enrichment of Salmonella enteritidis in turkey meat samples, Mid-SURE, Michigan State University East Lansing, MI. June 20, 2023 Caliskan-Aydogan, O., Sharief, S. A., & Alocilja, E. C. Carbohydrate-Coated Nanoparticles for PCR-less Detection of Salmonella from Chicken Meat. 2023 Engineering Graduate Research Symposium, Michigan State University, East Lansing, MI, May 11, 2023 Franco, A. J., Conklin, T, & Alocilja, E. 2023. Where Does Salmonella Come From? Preliminary Monitoring of the Presence of Salmonella at Various Stages of Poultry Processing. 2023 Engineering Graduate Research Symposium, Michigan State University East Lansing, MI, May 11, 2023. Recipient of Best Poster Award and First Place Data and Research Translation Award Hill D, Franco J, and Alocilja EC. 2023. AGEP Student Success Conference, Michigan State University, East Lansing, MI 48824, Nov. 9-11, 2023. 2nd Place Oral Presentation for undergraduates National Resources: Alocilja, E.C. 2023. Featured in Curious About Careers, Public Broadcasting Service (PBS) WKAR. Curious About Careers is a local public television program presented by WKAR. What do you plan to do during the next reporting period to accomplish the goals?Plans for next year include: Optimize the smartphone application for image display and analysis for the biosensing assay. Validate the magnetic enrichment in collaborators' farms & processing plants - extraction onsite. Validate the SMART biosensor in collaborators' farms & processing plants - biosensor tested onsite. Validate and compare the biosensor with Neogen's DNA kit. Generate data for temporal dynamics analysis. Calculate costs associated with the biosensor production and use in farms and processing plants. Calculate benefits associated with the biosensor technology compared with existing technologies. Assess the initial technology readiness of the biosensor. Meet with collaborators and other stakeholders to assess the significance of results and technology readiness.
Impacts
What was accomplished under these goals?
Objective 1: Optimize the Site-Enriched Multi-Array Reporting Biosensing Technology (SMART) for rapid detection of Salmonella and Campylobacter in large samples. Proposed Activity: Confirm target genes for S. enterica serovars and Campylobacter jejuni and C. coli detection. Accomplishments: The invA gene was the target for Salmonella enterica; Cj0415 and cadF genes were the targets for Campylobacter jejuni. Various molecular regions of the genomes of multiple Salmonella serovars were investigated to identify other potential target genes. Objective 2: Optimize cellphone-based data capture, analysis, and reporting of the biosensor signal output. Proposed Activity: Optimize app or image/data capture, analysis, and display. Accomplishments: Improvements to the smartphone application user interface and image analysis algorithm were implemented and validated. A database for data integration from various locations and collaborations is being set up. Objective 3: Validate the cellphone-enabled SMART biosensor for simultaneous detection of Salmonella spp. and Campylobacter spp. in selected poultry farms and slaughter facilities. Proposed Activity: Validate the magnetic enrichment in samples sent to the laboratory. Accomplishments: Samples were collected from the turkey and poultry processing plants, a turkey farm, and a chicken farm. Magnetic enrichment studies were conducted on various field samples such as poultry rinsate, manure, turkey swabs, and ground turkey meat. The extraction and concentration factors of the magnetic nanoparticles were determined. Inoculation was performed in samples with no bacterial growth to determine the extraction and concentration factors of the magnetic nanoparticles. Improvements in the procedure, such as lower enrichment volume and longer incubation time, are currently being conducted. Proposed Activity: Optimize and validate the SMART biosensor in samples sent to the laboratory. Accomplishments: Biosensor assays were performed on fecal, manure, and carcass swab samples. Proposed Activity: Determine the sensitivity and specificity of the biosensor. Accomplishments: Sensitivity and specificity were determined using multiple fecal samples (raw and artificially inoculated) to identify Salmonella and other priority pathogens from a complex matrix. Selectivity and sensitivity assays were performed in vitro using previously designed probes. Validation in additional probes is currently being performed. Proposed Activity: Determine the precision and accuracy of the biosensor. Accomplishments: Repeatability studies showed that the SMART biosensor signal was highly repeatable. More extensive assays at various repeatability conditions are ongoing. Proposed Activity: Benchmark existing tests in the market that are used by the poultry industry. Accomplishments: The material cost of the SMART biosensor was estimated and compared to the price of commercially available tests. The analysis time of the SMART biosensor was compared to that of the commercially available tests. Proposed Activity: Assess the capabilities and constraints of potential users of the biosensor. Accomplishments: Two undergraduate students were asked to use the SMART biosensor in some experiments. User feedback showed that the biosensor was simple to use.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Caliskan-Aydogan O, Sharief S, and Alocilja EC. 2023. Rapid Isolation of Low-level Carbapenem-Resistant E. coli from Water and Foods Using Glycan-Coated Magnetic Nanoparticles, Biosensors 2023, 13(10), 902.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Caliskan-Aydogan O and Alocilja EC. 2023. A Review of Carbapenem Resistance in Enterobacterales and Its Detection Techniques. Microorganisms, 2023, 11(6), 1491.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Boodoo C, Dester E, David J, Patel V, Rabin KC, and Alocilja EC. 2023. Multi-Probe Nano-Genomic Biosensor to Detect S. aureus from Magnetically-Extracted Food Samples, Biosensors, accepted, in press. Biosensors 2023, 13(6), 608.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Sharief S, Caliskan-Aydogan O, Alocilja EC. 2023. Carbohydrate-coated nanoparticles for PCR-less genomic detection of Salmonella from fresh produce, Food Control, Vol. 150, 109770.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Boodoo C, Dester E, Sharief S, and Alocilja EC. 2023. Influence of Biological and Environmental Factors in the Extraction and Concentration of Foodborne Pathogens using Glycan-Coated Magnetic Nanoparticles, ?Journal of Food Protection, 86(4):100066.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Sharief SA, Caliskan-Aydogan O, and Alocilja EC. 2023. Carbohydrate-coated nanoparticles for point-of-use food contamination testing, Biosensors and Bioelectronics: X, 13 (2023), 100322, 9 pp.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Caliskan-Aydogan O, Sharief S, and Alocilja EC. 2023. Nanoparticle-Based Plasmonic Biosensor for the Unamplified Genomic Detection of Carbapenem-Resistant Bacteria, Diagnostics, 2023, 13(4), 656.
- Type:
Journal Articles
Status:
Under Review
Year Published:
2024
Citation:
Ghazy A, Nyarku R, Faraj R, Bentum K, Woube Y, Williams M, Alocilja E, and Abebe W. Gold Nanoparticle Colorimetric Detection of Escherichia coli, Salmonella enterica, Campylobacter jejuni, and Listeria monocytogenes from bovine fecal Samples, Journal of Microbiology Research, in review.
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