Source: UNIVERSITY OF FLORIDA submitted to NRP
INVESTIGATE THE ROLES OF GUT MICROBIOME IN THE DIFFERENTIAL RESPONSES OF UTI SUSCEPTIBLE WOMEN TO CRANBERRY JUICE INTAKE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
AFRI COMPETITIVE GRANT
Reporting Frequency
Annual
Accession No.
1027766
Grant No.
2022-67017-36240
Cumulative Award Amt.
$650,000.00
Proposal No.
2021-09003
Multistate No.
(N/A)
Project Start Date
Jun 1, 2022
Project End Date
May 31, 2026
Grant Year
2022
Program Code
[A1343]- Food and Human Health
Recipient Organization
UNIVERSITY OF FLORIDA
G022 MCCARTY HALL
GAINESVILLE,FL 32611
Performing Department
Food Science & Human Nutrition
Non Technical Summary
The American cranberries, especially cranberry juice, have been used for centuries as a folk medicine to prevent urinary tract infections (UTI), which affect 50% of women in their lifetime. Over 30 clinical trials have been conducted in the last 20 years to verify the UTI preventatively activity of cranberry juices, but the results were contradictory. About 90% of UTI are initiated by the adhesion of uropathogenic E. coli (UPEC) on urinary tract epithelia. It was reported that human urine after consumption of cranberry juice inhibited the adhesion of UPEC. The A-type proanthocyanidins and xyloglucans are the presumed bioactives in cranberries, however, this is unlikely because these two classes of compounds have extremely low bioavailability in human body. Preliminary research suggested that women can be either "resistant" or "susceptible" to UTI depending on the inherent anti-adhesion activity in their urine against UPEC before consuming cranberry juice. Not all but a fraction of "UTI susceptible" women had increased urinary anti-adhesion activity after consuming cranberry juices. These women are classified as "responders" and others are "non-responders". The variation of gut microbiome is a probable mechanism for metabolic polymorphisms and disparity in UTI prevention. The objective of this trials is to identify gut microbes and anti-adhesive urinary biomarkers which significantly contribute to the anti-adhesion of UPEC.
Animal Health Component
25%
Research Effort Categories
Basic
75%
Applied
25%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
70111211010100%
Knowledge Area
701 - Nutrient Composition of Food;

Subject Of Investigation
1121 - Cranberry;

Field Of Science
1010 - Nutrition and metabolism;
Goals / Objectives
The objectives of this researchare to elucidate the differential impacts of cranberry juice consumption on the gut microbiome in women who respondeddifferently to cranberry juice consumption, and to explore the role of gut microbiota to catabolize cranberry bioactives into anti-adhesive metabolites in urine.
Project Methods
Wemen will avoid cranberry products for 7-10 days. Urine will be assessed for anti-adhesion activity against p-fimbriated E. coli using a fluorescent method. Womew with low or high baselineanti-ahesion acttivity will be classified as UTI susceptible or resistant. Susceptible women will be given cranberry juice for 4-5 days. Women with or withoutincreased urinary anti-adhesion activity will be classifed as responders and non-responders, respectively. Responders and non-responders will be given cranberry juice and apple juice using a crossever design. Anti-adhesion acitivity, gut microbiome, and urine metabolome will be assessed at baseline and post-consumption.

Progress 06/01/23 to 05/31/24

Outputs
Target Audience:Researchers and health professionals who are interested in the health benefits of cranberries. Changes/Problems:Our original method to measure the anti-adhesion activity of urine had a relatively large CV. It wasre-optimized to reduce the assay-to-assay variation. The day-to-day variation of anti-adhesion activitiesinhuman urine suggested that it isaffected by manycompounding factors, including different dietary factors, daily activities, etc. How to account for them remains a challenge. What opportunities for training and professional development has the project provided?This project provided financial support and training opportunity for one doctoral student. How have the results been disseminated to communities of interest?Results were presented atthe American Society of Nutrition Annual Meeting.Chicago, IL. June 29-July 2, 2024. What do you plan to do during the next reporting period to accomplish the goals?We will continue sample and data analysis.

Impacts
What was accomplished under these goals? Our results showed that urine metabolome in women with low urinary anti-adhesion activity against E. coli differed from those with high anti-adhesion activity.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2024 Citation: Shuhan Li, Timothy J Garrett, Manasi Kamat, Liwei Gu. Urine metabolome in women with low urinary anti-adhesion activity against E. coli differed from those with high anti-adhesion activity. Current Developments in Nutrition. 2024/7/1, 8.


Progress 06/01/22 to 05/31/23

Outputs
Target Audience:Cranberry consumers andbusinesses; women who are concerned about urinary tract health. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?This project provided training and support for a doctoral student. How have the results been disseminated to communities of interest?Results were presented at the American Society of Nutrition annual meeting in Boson MA, July 2023. What do you plan to do during the next reporting period to accomplish the goals?We need to refine our experimental methods to better control for variations in the anti-adhesion assay and the day-to-day variation of urinary anti-adhesion activity in participants. We also need to have a better understanding of other compounding factors that may affect urinary anti-adhesion activity.We will work on urinary metabolomics.

Impacts
What was accomplished under these goals? We tested the ability of urine to inhibit the adhesion of P-type uropathogenic E. coli in women before and after cranberry juice intake. Data suggested thatwomen have different UTI susceptibility and responses to cranberry juice intake.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Shuhan LI, Liwei Gu. Women have different UTI susceptibility and responses to cranberry juice intake based on urinary anti-adhesion activities against E. coli. American Society of Nutrition meeting. Boston, MA. July 2023.