Progress 09/01/22 to 08/31/23
Outputs
Target Audience:Target Audiences Served by GPDN States University personnel Extension agents Extension specialists Researchers College students (undergraduate and graduate) Industry Green industry professionals (arborists, turf specialists, landscaper, pesticide applicators), Greenhouse and landscape industries Crop consultants Plant Industry representatives (e.g., seed) Commercial pesticide applicators Government States Department of Agriculture employees USDA Animal and Plant Health Inspection Service Plant Protection and Quarantine Policy makers and elected officials (e.g., university legislative tours) General Public Master Gardeners Horticulture enthusiasts General public Girl scouts K-12 students Changes/Problems:Professional development coordinator and program leadership transition to Florida The NPDN Professional Development Coordinator position was based at the Great Plains Diagnostic Network Regional Center at Kansas State University. The position became vacant after the resignation of the PD coordinator following her maternity leave. Following two failed searches to fill that position, it was concluded that the best solution was to relocate the position to SPDN where someone who could assume the responsibilities of that position already existed. In subsequent annual contracts, the funding to support the position will go directly to SPDN and not require subawards from GPDN. This is a critical position with respect to NPDN's stated goals for this cooperative agreement; it is imperative that the position is filled with the best candidate available. That person was identified within the SPDN. The NPDN Mission Statement makes clear that to maintain a premier diagnostic system, our focus must be on quality diagnostics. To achieve that level of professionalism for all NPDN labs requires programs dedicated to constant quality improvement including, laboratory accreditation, diagnostician proficiency, and a professional development program to underpin all quality standards and to support diagnosticians. The NPDN Professional Development Program is integral to the implementation and subsequent success of NPDN's laboratory accreditation and diagnostician proficiency programs. Among the most important objectives for NPDN over the next three project years is for all NPDN diagnostic labs to achieve the NPDN CORE Laboratory Accreditation Standard. The CORE Standard ensures that all NPDN diagnostic labs are managed in a manner that promotes quality and ensures the high confidence in the diagnostic test results generated in that lab. Routine equipment calibrations, standard operating procedures, and constant training are critical features of the NPDN CORE Standard. The NPDN Professional Development Coordinator is an essential position to ensuring that the Professional Development Program develops and delivers the content needed to support NPDN diagnosticians in implementing the NPDN CORE Accreditation Standard for their labs and for achieving the NPDN Proficiency Standards. It was critical to fill that position quickly. What opportunities for training and professional development has the project provided?Professional Development: NPDN initiated a professional development program in 2019 to provide NPDN diagnosticians and staff training in an array of topics including, diagnostic technologies, e.g., PCR, and important pests and pathogens. That platform is also used to facilitate cross committee education. NPDN/GPDN partner with USDA APHIS PPQ to offer advanced diagnostic training by PPQ scientists regarding the official SOPs for regulated plant pathogens as well as training in the use of bioinformatics programs to assist in identifying pathogens. Potato Wart Workshop. Beltsville Training [March 29 2022; online]. 2 staff members. NPDN National Data Repository Data Use Policy Training. [Online]. 2 staff members. NPDN IT Diagnosticians Meeting. [January 26-27, 2022; online]. 1 staff member. NPDN Annual Meeting. [April 26-30, 2022; Davis, CA]. 2 staff member. Plant Health 2022. American Phytopathological Society. [August 12-16, 2022; Pittsburg, PA]. 1 staff member. Search chair training. Colorado State University [January 22, 2022; online]. 1 staff member. GPDN Webinar Series - multiple webcasts to all of NPDN labs and partner labs APS Webcast series Turf Conference K-State Garden Hour Biweekly Crop Condition Calls Horticulture PFT Hands-on diagnostic training at Oklahoma State University Hands-on diagnostic training at Texas A&M University NPDN National Meeting presentations NPDN National Meeting workshops Workshop, IsoThermal Amplification, USDA-APHIS-PPQ-S&T, (March 8, 2022). McKelvy, U. '" 1 staff Webinar. Inter-laboratory comparison testing for phytodiagnostic assays '" a key component of method validation?, Great Plains Diagnostic Network, (February 9, 2022). McKelvy, U., Grimme, E. '" 2 staff Webinar. The Price is Right: Calculating cost recovery in the diagnostic clinic, Great Plains Diagnostic Network, (February 2, 2022). McKelvy, U., Grimme, E. '" 2 staff Webinar. USDA APHIS PPQ State Plant Health Directors and Plant Pathologists- Who we are and what we do, Great Plains Diagnostic Network, (February 16, 2022). McKelvy, U., Grimme, E. '" 2 staff Webinar. Grasshopper Biology and Management. Great Plains Diagnostic Network, (February 23, 2022). Grimme, E. '" 1 staff Webinar. Assessment of Diaporthe species infecting sunflower for their sensitivity to tebuconazole fungicide in the greenhouse. Great Plains Diagnostic Network, (February 2, 2022). Grimme, E. '" 1 staff Webinar. Evaluation of the National Plant Diagnostic Network. Great Plains Diagnostic Network, (March 2, 2022). Grimme, E. '" 1 staff Webinar. BBIG, a team tackling boxwood blight- sharing progress on diagnostics, extension and outreach. Great Plains Diagnostic Network, (March 16, 2022). Grimme, E. '" 1 staff Webinar. PCR or ELISA? Choosing the appropriate viral detection method for diagnostics, Great Plains Diagnostic Network, (March 23, 2022). McKelvy, U., Grimme, E. '" 2 staff Conference Attendance. Propelling Diagnostics into the Future, National Plant Diagnostic Network, Davis, CA, USA, (April 26, 2022 - April 29, 2022). McKelvy, U., Grimme, E., Burrows, M. '" 3 staff Webinar. The nine things we wish we knew before we started teaching, American Phytopathological Society, (April 22, 2022 - Present). McKelvy, U., Grimme, E. '" 2 staff Conference Attendance. Annual Extension Fall Conference, MSU Extension, Bozeman, MT, United States, (October 17, 2022 - October 19, 2022). McKelvy, U., Grimme, E., Kerzicnik, L., Orloff, N., Rolston, M.'" 5 staff Conference Attendance. APS Plant Health 2022 - Warming Up to Change, American Phytopathological Society, Pittsburgh, PE, USA, (August 6, 2022 - August 10, 2022). McKelvy, U., Burrows, M. '" 2 staff Workshop. Mentoring in STEM: Constructing an Effective Mentoring Toolkit, American Phytopathological Society, Pittsburgh, PE, United States, (August 6, 2022). McKelvy, U. '" 1 staff Conference Attendance. Spring Ag/NR Update, MSU Extension, Bozeman, MT, USA, (May 10, 2022 - May 11, 2022). McKelvy, U., Grimme, E., Kerzicnik, L., Orloff, N.'" 4 staff Conference Attendance. 2022 International IPM Symposium, International IPM Symposium, Denver, CO, USA, (February 28, 2022 - March 3, 2022). McKelvy, U., Kerzicnik, L.'" 2 staff Conference Attendance. Pulse Crop Working Group Annual Meeting, Pulse Crop Working Group through the Northcentral IPM Center, Bozeman, MT, USA, (February 13, 2022 - February 15, 2022). McKelvy, U., Burrows, M. '" 2 staff Conference Attendance. North America Invasive Species Management Association Conference, Orlando, FL, 11/2022. Orloff, N.- 1 staff Grant Writing Bootcamp. Montana State University, Bozeman, MT. Jan '" Feb 2022. Kerzicnik, L. '" 1 staff Supervisor Training. Montana State University, Bozeman, MT. Mar '" Apr. 2022. Kerzicnik, L. '" 1 staff Conference Attendance. Montana Nursery and Landscape Association (MNLA), Billings, MT. 01/05/2022. Kerzicnik, L. '" 1 staff Conference Attendance. WERA 1017, Denver, CO, USA, (February 28, 2022). Kerzicnik, L.'" 1 staff Conference Attendance (virtual). 70th Western Forest Insect Work Conference. 04/27/2022. Kerzicnik, L. '" 1 staff Plant Parasitic Nematode course at Clemson University How have the results been disseminated to communities of interest?With respect to the following narrative, GPDN personnel is meant to include GPDN Regional Center staff, as well as, the diagnosticians and associated staff in each GPDN state. GPDN and NPDN personnel employ an array of communications technologies and delivery systems to ensure that accurate and timely communications reach target audiences with the information that they need to know when they need to know it. The target audiences include, farmers, consultants, plant industry specialists, university administrators, legislators, policy makers, homeowners (general public), K-12 students, undergraduate and graduate college students. GPDN personnel participate in multiple forum types that facilitate direct in-person contact with target audiences and virtual digital contacts with target audiences. This approach facilitates the broadest reach of GPDN states and achieves the greatest impacts with respect to the primary objectives of GPDN and NPDN. For most routine operations, GPDN states utilize open communications platforms to reach the most clientele. Diagnostic reports are issued to clientele that submit samples to the individual GPDN state diagnostic labs, often with recommendations for mitigation. GPDN diagnosticians communicate with state regulatory officials if a sample if of regulatory concern. When working with regulatory/high consequence samples with the potential for substantial economic or trade impacts, GPDN personnel utilize restricted (secure) mechanisms of communication to protect that information. GPDN personnel work closely with state and federal regulatory agencies to assist during state and/or national plant health emergencies. With respect to specific mechanisms of information dissemination, GPDN personnel participate in growers' meetings, field day events, regularly scheduled and intermittent TV and radio interviews, blogs, newsletters, individual diagnostic lab reports to clientele, presentations at scientific meetings (posters and oral), podcasts, diagnostic lab web pages, and some NPDN web resources. Please see the list of dissemination events in the products sections for examples of specific dissemination activities and mechanisms. Diagnostic records are uploaded to the NPDN National Data Repository (NDR). The NPDN NDR has restricted access; only NPDN personnel have direct access to the NPDN NDR. Stakeholders and scientist can request focused datasets from the NPDN NDR and if approved, will be provided with a data report; they will not be granted direct access to the NDR. What do you plan to do during the next reporting period to accomplish the goals?The objectives for project year 2023-2024 remain the same as in the cooperative agreement. This is a continuing project. The goals will be accomplished through a continuation of the programs in place and the activities performed within the individual state labs and at the Regional Center. Objectives for project year 2023-2024 Professional development USDA PPQ Confirmatory Diagnostic Laboratory Workshops: diagnostic trainings NPDN and other professional development activities Diagnostics: Surveys, passive and active surveillance Diagnostic activity in NPDN labs: Data upload to NPDN NDR by NPDN labs Lab accreditation activities Communications and Reporting IT/Diagnosticians' meeting Communicate with regulatory officials (SPRO, SPHD, CDL) Participate at the GPDN Regional meeting
Impacts
What was accomplished under these goals?
GPDN Objectives for 2022: Objective 1: Professional development Completion: 100% Significant accomplishments, outcomes, and impacts: GPDN diagnosticians completed advanced diagnostic trainings at the USDA PPQ Confirmatory Diagnostic Laboratory in Laurel, Maryland GPDN diagnosticians participated in the GPDN webinar series providing detailed knowledge on a range of relevant topics other GPDN diagnosticians completed NPDN professional development activities including, update training on the NPDN NDR Objective 2: Diagnostics Completion: 90% Significant accomplishments, outcomes, and impacts: GPDN diagnosticians participated in trace-back and trace-forward surveillance for two plant health emergencies for the Ralstonia solanacearum r3b2 and Phytophthora ramorum events. GPDN diagnostic labs provided diagnostic services for 10,698 samples and contributed 7509 diagnostic records to the NPDN National Data Repository Objective 3: Communications and Reporting Completion: 100% Significant accomplishments, outcomes, and impacts: GPDN diagnosticians communicated with state and federal regulatory officials in support of response activities for two plant health emergencies GPDN diagnosticians participated at the GPDN Regional meeting providing state reports of diagnostic activities for the previous season and observations on new or unusual occurrences of pests and pathogens. All GPDN states were present at the GPDN Regional Meeting and shared experience and knowledge regarding new technologies and methods. 1. Total number of peer-reviewed publications: 14 peer-reviewed publications from GPDN 2. Total number of non-peer-reviewed publications: 22 non-peer-reviewed publications from GPDN 3. Total number of presentations: at least 140 presentations by GPDN states 4. Total number of people reached by your project during this reporting period: 8-10,000
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
X. Lai, D. Niroula, M. Burrows, X. Wu, Q. Yan. 2022. Identification and characterization of bacteria-derived antibiotics for the biological control of pea Aphanomyces root rot. Microorganisms. 10:1596. DOI: 10.3390/microorganisms10081596
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
McKelvy, U., M. Brelsford, M. Burrows. 2022. Evaluation of seed transmission rates of wheat streak mosaic virus in mechanically inoculated winter and spring wheat cultivars in Montana. Plant Disease. (accepted).
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Moparthi, S., L. Parikh, E. Gunnik Troth, M. Burrows. 2022. Identification and prevalence of seedborne Botrytis spp. in dry pea, lentil, and chickpea in Montana. Plant Disease. DOI: 10.1094/PDIS-05-22-1236-RE
- Type:
Other
Status:
Published
Year Published:
2022
Citation:
Dyer, A., Tharp, C., Johnston, J., McKelvy, U., Lane, T., Fulbright, J., Rupp, J. L. (2022). In MSU Extension Publications (Ed.), Small Grain Seed Treatment Guide (MT199608AG ed., pp. 8). Bozeman, Montana: MSU Extension Publications / MontGuide. https://store.msuextension.org/publications/AgandNaturalResources/MT199608AG.pdf
- Type:
Other
Status:
Published
Year Published:
2022
Citation:
Kalil, A., McKelvy, U., & Porter, L. (Eds.). [2022]. Chickpea Disease Diagnostic Series. North Dakota State University Extension Publication. https://www.ndsu.edu/agriculture/extension/publications/chickpea-disease-diagnostic-series
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Peterson, R., Rolston, M. 2022. Larval mosquito management and risk to aquatic ecosystems: A comparative approach including current tactics and gene-drive Anopheles techniques. SpringerLink. DOI: 10.1007/s11248-022-00315-9
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Morey, AC, LM Kerzicnik, FE Etzler, K. Mendrey, BD Morey, Z. Miller. 2022. First report of Halyomorpha halys (Hemiptera: Petatomidae) in Montana, USA. Journal of Integrated Pest Management 13(1): 27; 1-4. https://doi.org/10.1093/jipm/pmac024
- Type:
Other
Status:
Published
Year Published:
2022
Citation:
Kerzicnik, LM, SM Vantassel, C. Lay. 2022. Industrial, institutional, structural, and health related training manual. Helena, MT: Montana Department of Agriculture. 139 pp
- Type:
Other
Status:
Accepted
Year Published:
2022
Citation:
Kerzicnik, L. et al. 2022. Insects and mites of trees of the Great Plains. In press
- Type:
Book Chapters
Status:
Other
Year Published:
2022
Citation:
Vazquez-Iglesias, I., Delmiglio, C., Ochoa-Corona, F.M., Thompson, J.R., Olson, J.D., Clover, G., Boonham, N. and Fox, A. Virus diseases of rose plants. Submitted 01/2022: Book Chapter
- Type:
Other
Status:
Published
Year Published:
2022
Citation:
Nicolas Aparicio Claros, Madalyn Shires, Dimitre Mollov, John Hammond, Ramon Jordan, Francisco Ochoa-Corona, Jennifer Olson, Kevin Ong, and Lina Rodriguez Salamanca 2022. Rose Rosette Disease: A Diagnostic Guide. Plant Health Progress 2022 23:4, 482-491
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
DeLude, A., Wells, R., Boomla, S. Chuang, SC, Urena, F., Shipman, A., Rubas, N., Kuehu, D.L., Bickerton, B., Peterson, T., Dobhal, S., Arizala, D., Klair, D., Ochoa-Corona, F., Ali, M.E., Odani, J., Bingham, J.P., Jenkins, D., Fletcher, J., Stack, J.P., Alvarez, A.M., and Arif, M. 2022 Loop-mediated isothermal amplification (LAMP) assay for specific and rapid detection of Dickeya fangzhongdai targeting a unique genomic region. Sci Rep 12, 19193 (2022). https://doi.org/10.1038/s41598-022-22023-4
|
Progress 09/01/21 to 08/31/23
Outputs
Target Audience:Target Audiences Served by GPDN States University personnel Extension agents Extension specialists Researchers College students (undergraduate and graduate) Industry Green industry professionals (arborists, turf specialists, landscaper, pesticide applicators), Greenhouse and landscape industries Crop consultants Plant Industry representatives (e.g., seed) Commercial pesticide applicators Government States Department of Agriculture employees USDA Animal and Plant Health Inspection Service Plant Protection and Quarantine Policy makers and elected officials (e.g., university legislative tours) General Public Master Gardeners Horticulture enthusiasts General public Girl scouts K-12 students Changes/Problems:Professional development coordinator and program leadership transition to Florida The NPDN Professional Development Coordinator position was based at the Great Plains Diagnostic Network Regional Center at Kansas State University. The position became vacant after the resignation of the PD coordinator following her maternity leave. Following two failed searches to fill that position, it was concluded that the best solution was to relocate the position to SPDN where someone who could assume the responsibilities of that position already existed. That transition was made and the new Professional Development Corrdinator has been hired. In subsequent annual contracts, the funding to support the position will go directly to SPDN and not require subawards from GPDN. This is a critical position with respect to NPDN's stated goals for this cooperative agreement; it is imperative that the position is filled with the best candidate available. That person was identified within the SPDN. The NPDN Mission Statement makes clear that to maintain a premier diagnostic system, our focus must be on quality diagnostics. To achieve that level of professionalism for all NPDN labs requires programs dedicated to constant quality improvement including, laboratory accreditation, diagnostician proficiency, and a professional development program to underpin all quality standards and to support diagnosticians. The NPDN Professional Development Program is integral to the implementation and subsequent success of NPDN's laboratory accreditation and diagnostician proficiency programs. Among the most important objectives for NPDN over the next three project years is for all NPDN diagnostic labs to achieve the NPDN CORE Laboratory Accreditation Standard. The CORE Standard ensures that all NPDN diagnostic labs are managed in a manner that promotes quality and ensures the high confidence in the diagnostic test results generated in that lab. Routine equipment calibrations, standard operating procedures, and constant training are critical features of the NPDN CORE Standard. The NPDN Professional Development Coordinator is an essential position to ensuring that the Professional Development Program develops and delivers the content needed to support NPDN diagnosticians in implementing the NPDN CORE Accreditation Standard for their labs and for achieving the NPDN Proficiency Standards. It was critical to fill that position quickly. What opportunities for training and professional development has the project provided?Professional Development: NPDN initiated a professional development program in 2019 to provide NPDN diagnosticians and staff training in an array of topics including, diagnostic technologies, e.g., PCR, and important pests and pathogens. That platform is also used to facilitate cross committee education. NPDN/GPDN partner with USDA APHIS PPQ to offer advanced diagnostic training by PPQ scientists regarding the official SOPs for regulated plant pathogens as well as training in the use of bioinformatics programs to assist in identifying pathogens. Potato Wart Workshop. Beltsville Training [March 29 2022; online]. 2 staff members. NPDN National Data Repository Data Use Policy Training. [Online]. 2 staff members. NPDN IT Diagnosticians Meeting. [January 26-27, 2022; online]. 1 staff member. NPDN Annual Meeting. [April 26-30, 2022; Davis, CA]. 2 staff member. Plant Health 2022. American Phytopathological Society. [August 12-16, 2022; Pittsburg, PA]. 1 staff member. Search chair training. Colorado State University [January 22, 2022; online]. 1 staff member. GPDN Webinar Series - multiple webcasts to all of NPDN labs and partner labs APS Webcast series Turf Conference K-State Garden Hour Biweekly Crop Condition Calls Horticulture PFT Hands-on diagnostic training at Oklahoma State University Hands-on diagnostic training at Texas A&M University NPDN National Meeting presentations NPDN National Meeting workshops Workshop, IsoThermal Amplification, USDA-APHIS-PPQ-S&T, (March 8, 2022). McKelvy, U. '" 1 staff Webinar. Inter-laboratory comparison testing for phytodiagnostic assays '" a key component of method validation?, Great Plains Diagnostic Network, (February 9, 2022). McKelvy, U., Grimme, E. '" 2 staff Webinar. The Price is Right: Calculating cost recovery in the diagnostic clinic, Great Plains Diagnostic Network, (February 2, 2022). McKelvy, U., Grimme, E. '" 2 staff Webinar. USDA APHIS PPQ State Plant Health Directors and Plant Pathologists- Who we are and what we do, Great Plains Diagnostic Network, (February 16, 2022). McKelvy, U., Grimme, E. '" 2 staff Webinar. Grasshopper Biology and Management. Great Plains Diagnostic Network, (February 23, 2022). Grimme, E. '" 1 staff Webinar. Assessment of Diaporthe species infecting sunflower for their sensitivity to tebuconazole fungicide in the greenhouse. Great Plains Diagnostic Network, (February 2, 2022). Grimme, E. '" 1 staff Webinar. Evaluation of the National Plant Diagnostic Network. Great Plains Diagnostic Network, (March 2, 2022). Grimme, E. '" 1 staff Webinar. BBIG, a team tackling boxwood blight- sharing progress on diagnostics, extension and outreach. Great Plains Diagnostic Network, (March 16, 2022). Grimme, E. '" 1 staff Webinar. PCR or ELISA? Choosing the appropriate viral detection method for diagnostics, Great Plains Diagnostic Network, (March 23, 2022). McKelvy, U., Grimme, E. '" 2 staff Conference Attendance. Propelling Diagnostics into the Future, National Plant Diagnostic Network, Davis, CA, USA, (April 26, 2022 - April 29, 2022). McKelvy, U., Grimme, E., Burrows, M. '" 3 staff Webinar. The nine things we wish we knew before we started teaching, American Phytopathological Society, (April 22, 2022 - Present). McKelvy, U., Grimme, E. '" 2 staff Conference Attendance. Annual Extension Fall Conference, MSU Extension, Bozeman, MT, United States, (October 17, 2022 - October 19, 2022). McKelvy, U., Grimme, E., Kerzicnik, L., Orloff, N., Rolston, M.'" 5 staff Conference Attendance. APS Plant Health 2022 - Warming Up to Change, American Phytopathological Society, Pittsburgh, PE, USA, (August 6, 2022 - August 10, 2022). McKelvy, U., Burrows, M. '" 2 staff Workshop. Mentoring in STEM: Constructing an Effective Mentoring Toolkit, American Phytopathological Society, Pittsburgh, PE, United States, (August 6, 2022). McKelvy, U. '" 1 staff Conference Attendance. Spring Ag/NR Update, MSU Extension, Bozeman, MT, USA, (May 10, 2022 - May 11, 2022). McKelvy, U., Grimme, E., Kerzicnik, L., Orloff, N.'" 4 staff Conference Attendance. 2022 International IPM Symposium, International IPM Symposium, Denver, CO, USA, (February 28, 2022 - March 3, 2022). McKelvy, U., Kerzicnik, L.'" 2 staff Conference Attendance. Pulse Crop Working Group Annual Meeting, Pulse Crop Working Group through the Northcentral IPM Center, Bozeman, MT, USA, (February 13, 2022 - February 15, 2022). McKelvy, U., Burrows, M. '" 2 staff Conference Attendance. North America Invasive Species Management Association Conference, Orlando, FL, 11/2022. Orloff, N.- 1 staff Grant Writing Bootcamp. Montana State University, Bozeman, MT. Jan '" Feb 2022. Kerzicnik, L. '" 1 staff Supervisor Training. Montana State University, Bozeman, MT. Mar '" Apr. 2022. Kerzicnik, L. '" 1 staff Conference Attendance. Montana Nursery and Landscape Association (MNLA), Billings, MT. 01/05/2022. Kerzicnik, L. '" 1 staff Conference Attendance. WERA 1017, Denver, CO, USA, (February 28, 2022). Kerzicnik, L.'" 1 staff Conference Attendance (virtual). 70th Western Forest Insect Work Conference. 04/27/2022. Kerzicnik, L. '" 1 staff Plant Parasitic Nematode course at Clemson University How have the results been disseminated to communities of interest?With respect to the following narrative, GPDN personnel is meant to include GPDN Regional Center staff, as well as, the diagnosticians and associated staff in each GPDN state. GPDN and NPDN personnel employ an array of communications technologies and delivery systems to ensure that accurate and timely communications reach target audiences with the information that they need to know when they need to know it. The target audiences include, farmers, consultants, plant industry specialists, university administrators, legislators, policy makers, homeowners (general public), K-12 students, undergraduate and graduate college students. GPDN personnel participate in multiple forum types that facilitate direct in- person contact with target audiences and virtual digital contacts with target audiences. This approach facilitates the broadest reach of GPDN states and achieves the greatest impacts with respect to the primary objectives of GPDN and NPDN. For most routine operations, GPDN states utilize open communications platforms to reach the most clientele. Diagnostic reports are issued to clientele that submit samples to the individual GPDN state diagnostic labs, often with recommendations for mitigation. GPDN diagnosticians communicate with state regulatory officials if a sample if of regulatory concern. When working with regulatory/high consequence samples with the potential for substantial economic or trade impacts, GPDN personnel utilize restricted (secure) mechanisms of communication to protect that information. GPDN personnel work closely with state and federal regulatory agencies to assist during state and/or national plant health emergencies. With respect to specific mechanisms of information dissemination, GPDN personnel participate in growers' meetings, field day events, regularly scheduled and intermittent TV and radio interviews, blogs, newsletters, individual diagnostic lab reports to clientele, presentations at scientific meetings (posters and oral), podcasts, diagnostic lab web pages, and some NPDN web resources. Please see the list of dissemination events in the products sections for examples of specific dissemination activities and mechanisms. Diagnostic records are uploaded to the NPDN National Data Repository (NDR). The NPDN NDR has restricted access; only NPDN personnel have direct access to the NPDN NDR. Stakeholders and scientist can request focused datasets from the NPDN NDR and if approved, will be provided with a data report; they will not be granted direct access to the NDR. What do you plan to do during the next reporting period to accomplish the goals?This is the final report for this cooperative agreement. A new cooperative agreement with appropriate objectives will be established.
Impacts
What was accomplished under these goals?
Accomplishments As one of the National Plant Diagnostic Network's (NPDN) five regional diagnostic networks, the Great Plains Diagnostic Network (GPDN) is a key component of the nation's plant biosecurity infrastructure. As such, the major goals of GPDN are 1) to provide quality and timely diagnostic services to sustain plant health and support stakeholder communities, 2) to provide diagnostic support to state and federal regulatory agencies during plant health emergencies, 3) to contribute diagnostic information to the NPDN National Data Repository, and 4) to provide educational services to stakeholder communities in order to facilitate early detection of organisms potentially harmful to natural and agricultural plant systems. These goals for GPDN are to fully support the three central strategic program areas identified by NIFA: 1) Detection and first detector training, 2) Diagnostic activities, and 3) Reporting using IT systems. The GPDN Regional Center will provide regional IT support (e.g., database maintenance and support for diagnostic data management and upload to the national repository, GPDN portal management), regional diagnostic support (e.g., technical and sample surge support, development and distribution of diagnostic and communication Standard Operating Procedures), regional coordination and management of the outbreak preparedness exercise program, and administrative management of the regional Network. What was accomplished under these goals? GPDN Objectives for 2022: Objective 1: Professional development Completion: 100% Significant accomplishments, outcomes, and impacts: • GPDN diagnosticians completed advanced diagnostic trainings at the USDA PPQ Confirmatory Diagnostic Laboratory in Laurel, Maryland • GPDN diagnosticians participated in the GPDN webinar series providing detailed knowledge on a range of relevant topics other • GPDN diagnosticians completed NPDN professional development activities including, update training on the NPDN NDR Objective 2: Diagnostics Completion: 90% Significant accomplishments, outcomes, and impacts: • GPDN diagnosticians participated in trace-back and trace-forward surveillance for two plant health emergencies for the Ralstonia solanacearum r3b2 and Phytophthora ramorum events. • GPDN diagnostic labs provided diagnostic services for 10,698 samples and contributed 7509 diagnostic records to the NPDN National Data Repository Objective 3: Communications and Reporting Completion: 100% Significant accomplishments, outcomes, and impacts: • GPDN diagnosticians communicated with state and federal regulatory officials in support of response activities for two plant health emergencies • GPDN diagnosticians participated at the GPDN Regional meeting providing state reports of diagnostic activities for the previous season and observations on new or unusual occurrences of pests and pathogens. All GPDN states were present at the GPDN Regional Meeting and shared experience and knowledge regarding new technologies and methods. 1. Total number of peer-reviewed publications: 14 peer-reviewed publications from GPDN 2. Total number of non-peer-reviewed publications: 22 non-peer-reviewed publications from GPDN 3. Total number of presentations: at least 140 presentations by GPDN states 4. Total number of people reached by your project during this reporting period: 8-10,000
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
X. Lai, D. Niroula, M. Burrows, X. Wu, Q. Yan. 2022. Identification and characterization of bacteria-derived antibiotics for
the biological control of pea Aphanomyces root rot. Microorganisms. 10:1596. DOI: 10.3390/microorganisms10081596
McKelvy, U., M. Brelsford, M. Burrows. 2022. Evaluation of seed transmission rates of wheat streak mosaic virus in
mechanically inoculated winter and spring wheat cultivars in Montana. Plant Disease. (accepted).
Moparthi, S., L. Parikh, E. Gunnik Troth, M. Burrows. 2022. Identification and prevalence of seedborne Botrytis spp. in dry pea, lentil, and chickpea in Montana. Plant Disease. DOI: 10.1094/PDIS-05-22-1236-RE
Dyer, A., Tharp, C., Johnston, J., McKelvy, U., Lane, T., Fulbright, J., Rupp, J. L. (2022). In MSU Extension Publications
(Ed.), Small Grain Seed Treatment Guide (MT199608AG ed., pp. 8). Bozeman, Montana: MSU Extension Publications/MontGuide. https://store.msuextension.org/publications/AgandNaturalResources/MT199608AG.pdf
Kalil, A., McKelvy, U., & Porter, L. (Eds.). [2022]. Chickpea Disease Diagnostic Series. North Dakota State University Extension Publication. https://www.ndsu.edu/agriculture/extension/publications/chickpea-disease-diagnostic-series
Peterson, R., Rolston, M. 2022. Larval mosquito management and risk to aquatic ecosystems: A comparative approach including current tactics and gene-drive Anopheles techniques. SpringerLink. DOI: 10.1007/s11248-022-00315-9
Morey, AC, LM Kerzicnik, FE Etzler, K. Mendrey, BD Morey, Z. Miller. 2022. First report of Halyomorpha halys (Hemiptera: Petatomidae) in Montana, USA. Journal of Integrated Pest Management 13(1): 27; 1-4
Kerzicnik, LM, SM Vantassel, C. Lay. 2022. Industrial, institutional, structural, and health related training manual. Helena, MT: Montana Department of Agriculture. 139 pp
Kerzicnik, L. et al. 2022. Insects and mites of trees of the Great Plains. In press
Vazquez-Iglesias, I., Delmiglio, C., Ochoa-Corona, F.M., Thompson, J.R., Olson, J.D., Clover, G., Boonham, N. and Fox, A. Virus diseases of rose plants. Submitted 01/2022: Book Chapter
Nicolas Aparicio Claros, Madalyn Shires, Dimitre Mollov, John Hammond, Ramon Jordan, Francisco Ochoa-Corona, Jennifer Olson, Kevin Ong, and Lina Rodriguez Salamanca 2022. Rose Rosette Disease: A Diagnostic Guide. Plant Health Progress 2022 23:4, 482-491
DeLude, A., Wells, R., Boomla, S. Chuang, SC, Urena, F., Shipman, A., Rubas, N., Kuehu, D.L., Bickerton, B., Peterson, T., Dobhal, S., Arizala, D., Klair, D., Ochoa-Corona, F., Ali, M.E., Odani, J., Bingham, J.P., Jenkins, D., Fletcher, J., Stack, J.P., Alvarez, A.M., and Arif, M. 2022 Loop-mediated isothermal amplification (LAMP) assay for specific and rapid detection of Dickeya fangzhongdai targeting a unique genomic region. Sci Rep 12, 19193 (2022). https://doi.org/10.1038/s41598-022-22023-4
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Progress 09/01/21 to 08/31/22
Outputs
Target Audience:Target audiences reached this past year included large and small-scale agricultural producers, homeowners, plant production and nursery operators, diagnosticians, state (Departments of Agriculture) and federal (USDA APHIS Plant Protection and Quarantine) plant regulatory personnel, and plant industry (nursery, seed) support professionals. The Great Plains Diagnostic Network (GPDN) is one of five regional networks of the National Plant Diagnsotic Network and is comprised of representatives of Land Grant Universities and Agricultural Experiment Stations from the following nine states; Colorado, Kansas, Montana, Nebraska, North Dakota, Oklahoma, South Dakota, Texas, and Wyoming. GPDN is comprised of a team of specialists skilled at rapid and accurate identification of plant pests and pathogens, timely communications of diagnostic test results, and the delivery of training programs to facilitate early detection and mitigation of pest and disease outbreaks that impact large and small-scale agrilcutultural production systems, commercial horticultural production and retail systems, as well as homeowner environments. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Total number of training sessions and total number of First Detectors trained: GPDN members conducted many training sessions for First Detectors. The number of sessions conducted and the number of First Detectors trained are included in the total numbers presented in Table 1. At least 3,109 First Detectors received some first detector training at with least 60 presentations/publications. Most First Detector Expanded Awareness presentations occurred during sessions at which GPDN members were invited to speak, but were not necessarily organized by GPDN members; consequently, registration of attendees did not occur. Summary of regional training efforts: Christian Webb (Kansas) served as the GPDN regional Training and Education Coordinator and serves on the National Training and Education Committee (Rachel McCarthy, Chair). State training coordinators are Ana Cristina Fulladolsa (CO), Jim Stack & Judy O'Mara (KS), Mary Burrows (MT), Kyle Broderick (NE), Jesse Ostrander (ND), Jen Olson (OK), Emmanuel Byamukama (SD), Ken Obasa (TX), and William Stump (WY). How have the results been disseminated to communities of interest? Summary of publications and presentations resulting from training efforts and summary of training/ First Detector highlights/success stories: A regional webinar series continued this year to provide GPDN states with the most recent information on several emerging and remerging pathogens and insect pests. First Detector training sessions for Master Gardeners were conducted in several GPDN states. Montana produced several plant disease and pest awareness programs that were widely televised throughout the state estimated to have reached over 150,000 people. Summary of publications and presentations resulting from training efforts and summary of training/ First Detector highlights/success stories: A regional webinar series continued this year to provide GPDN states with the most recent information on several emerging and remerging pathogens and insect pests. First Detector training sessions for Master Gardeners were conducted in several GPDN states. A First Detector training occurred at the Cheyenne Botanical Gardens (WY) where Kyle Broderick (NE), Chandler Day (KS), and Dr. Whitney Cranshaw (CO). This training was in conjunction with Sentinel Plant Network (SPN) and the American Public Gardens Association where 35 individuals from 14 public garden within the Great Plains received pest awareness training. Montana produced several plant disease and pest awareness programs that were widely televised throughout the state. How have the results been disseminated to communities of interest?Results were communicated to communities of interest through a variety of delivery vehicles including, web-based newsletters, conference presentations, town hall meeting, seminars, webinars, radio interviews, podcasts, and extension outreach and training programs. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Summary of Accomplishments and Regional Highlights 1. Detection: Detection accomplishment in the GPDN included, but were not limited to, Colorado detected Alfalfa mosaic virus in pepper (Capsicum annum), Botrytis cinerea in hemp (Cannabis sativa), Setomelanomma holmii in spruce (Picea pungens), Hop latent viroid in hemp (Cannabis sativa), and Parthenothrips dracaena on Schefflera sp. Kansas provided diagnostic support to the Kansas Department of Agricultural boxwood blight survey and trace forwards/back for Phytophthora ramorum infect nursery stock. Montana detected Globe thistle (Echinops schaerocephalus), Partridge pea (Chamaecrista fasciculata), Botrytis euroamericana and Botrytis prunorum found on chickpea (Cicer arietinum), white rust on mustard (Albuga candida) and could be an important pathogen of canola. Three bed bug workshops were conducted where 82% of participants increased their knowledge about detecting bed bug infestations, 86% increased their knowledge about bed bug biology, and 73% were likely to make monitoring/inspection changes for bed bugs based of the information learned at the workshops. Nebraska taught graduate and undergraduate students to look at and diagnose plant health problems. The graduate students gained a better understanding of economically important plant pathogens and the ability to diagnose plants in the field. North Dakota detected Boxwood Blight (Calonectria pseudonaviculata) and passed an audit for Cms testing for potato bacterial ring rot entry into Canada. Oklahoma worked with Oklahoma Department of Agriculture to detect Phytophthora ramorum in a large local nursery and garden centers and detected brown marmorated stink bug (Halyomorpha halys), American Plum Line Pattern Virus, Little Cherry 2 virus, and Hammerhead Flatworm (Bipalium sp.). A Direct Trapping of Viruses on Plastic method was taught in a hands-on workshop to 16 members of the GPDN in a workshop in October 2019 and a webinar with instructional videos was presented as part of the GPDN webinar series on February 12, 2020. South Dakota detected Phytophthora sansomeana on soybeans, bacterial leaf streak (Xanthomonas vasicola pv. Vasculorum) on corn. Texas identified 13 candidate bacterial consortia from corn samples collected from Texas, Kansas, and Colorado states with potential for mitigation of fumonisin production in corn. Wyoming had a first detectors training at the Cheyenne Botanical Gardens that was hosted in conjunction with the Sentinel Plant Network. 2. Diagnosis: GPDN accomplishments in diagnosis included, but were not limited to, Colorado adopted a PCR assay from pesticded-coated seeds to detect Synchytrium endobioticum in tomato seeds. Ditylenchus dipsaci was able to be diagnosed using newly adpoted nematode extraction and identification methods. A PCR bacterial isolation assay from seed was optimized to diagnose Pseudomonas cannabina in hemp seed and Xanthomonas cannabis detection in hemp seed. A freezer blotter method was adopted to diagnose Fusarium sp. detection in hemp seed using fungal isolation, PCR of the ITS region and subsequent Sanger sequencing were used to identify the species. The same freezer blotter method was adopted to detect Leptosphaeria woroninii in hemp seed. A method for DNA extraction and PCR assay from hemp seeds was developed to detect Ramularia collo-cygni in hemp seed. A method combining qPCR and a tomato bioassay was developed and adopted for Spongospora subterranea detection in potting mix.A high-throughput method for DNA extraction and PCR assay from hemp leaf tissue was developed for Beet curly top virus detection in hemp. An optimized (by another diagnostic lab) method based on the RT-PCR assay to detect Hop latent viroid in hemp. Kansas developed two SOP for handling and processing hemp samples and sudden oak death survey samples. An additional 1,330 digital samples were address between the diagnostic lab and the horticulture rapid response center. Montana saved over $4.9 million affecting approximately 190,000 acres as a result of SDL recommendations. Nebraska serves as the Nebraska Department of Agriculture's diagnostic resource for nursery and crop inspectors and is increasing their diagnostic capabilities by purchasing a PCR workstation. North Dakota helps agricultural producers and professionals, horticulture and urban forestry professionals, university researchers and homeowners identify plant pests, one of the keys of Integrated Pest Management. They provided management advice regarding plant pathogens or diseases for approximately 500 disease identification requests and processed approximately 400 insect identification requests, of which approximately 90% of submitters were receptive to implementing appropriate integrated pest management strategies to control their respective insect pests. They also have started testing soil for Pot Mop-Top Virus. Oklahoma adopted a new SOP for detection of Meloidogyne spp. and M. enterolobii. This is a combination of traditional nematode extraction using sugar flotation centrifugation and molecular tests on individual nematodes. An 18-month evaluation was successful validated for Direct Trapping of Viruses on Plastic that it is effective for rose rosette virus, cucumber mosaic, Poty virus group, tobacco rattle and tobacco mosaic viruses. The method has reduced costs of DNA extraction by about 65%. An AmpliFire from Agdia was purchased and has been successfully used to test for Xylella fastidiosa and Grapevine Red Blotch Virus. An experimental protocol from researchers using isothermal amplification equipment is being used for a project on Rose rosette virus (RRV) and continuing to be evaluated. Overall the AmpliFire has reduced the time for testing RRV samples from 6-8 hours down to 30 minutes and the cost of the test is also much lower as a result of less expensive reagents and reduced labor. South Dakota identified a first detection of Phytophthora sansomeana, a new root rot pathogen of soybean in South Dakota. Texas added mycotoxin testing to the services now offered by the lab for the following mycotoxins - DON, Fumonisin, Aflatoxin, Ochratoxin, Zearalenone, and T- 2/HT-2 and is accredited to offer mycotoxin testing services by the Office of the Texas State Chemist. Wyoming diagnosed 41 submitted samples from a wide range of plant types to include ornamental plants, field crops, turf, vegetables and fruits. The following pest categories diagnoses were found; arthropods 4, pathogens 16, abiotic disorders 14, nematodes 4, and 3 others. 3. Reporting: GPDN accomplishments in reporting included, but were not limited to, Kansas' real time deposits into the National repository. Kansas also processed 612 samples and diagnosed 115 abiotic disorders, 26 arthropods, 116 nematodes, 300 pathogens, and 1 plant/weed. Colorado processed 704 samples and diagnosed 48 abiotic disorders, 62 arthropods, 6 nematodes, 191 pathogens, and 5 plants/weeds. Montana processed 1,373 samples and diagnosed 407 abiotic disorders, 576 arthropods, 2 nematodes, 641 pathogens, 235 plants/weeds. Nebraska providing weekly updates to the Crop Watch newsletter and diagnosed 96 abiotic disorders, 57 arthropods, 14 nematodes, and 685 pathogens. North Dakota processed 2,341 and diagnosed 75 abiotic disorders, 243 arthropods, 910 nematodes. 486 pathogens, and 17 plants/weeds. Oklahoma uploaded all data to the NPDN repository. For this time period, they uploaded data for 3,741 samples. South Dakota processed 117 samples and diagnosed 28 abiotic disorders, 16 arthropods, 21 nematodes, 107 pathogens, and 6 plants/weeds. Texas processed 77 samples and diagnosed 171 pathogens, 17 arthropods, and 26 abiotic disorders. Wyoming processed 18 sample and diagnosed 41 pests.
Publications
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