Source: UNIV OF WISCONSIN submitted to NRP
ACQUISITION OF UHPLC-MS/MS FOR ADVANCING RESEARCH ON FOOD AND HEALTH.
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
OTHER GRANTS
Reporting Frequency
Annual
Accession No.
1026838
Grant No.
2021-70410-35294
Cumulative Award Amt.
$496,719.00
Proposal No.
2021-05066
Multistate No.
(N/A)
Project Start Date
Jul 1, 2021
Project End Date
Jun 30, 2023
Grant Year
2021
Program Code
[EGP]- Equipment Grants Program
Recipient Organization
UNIV OF WISCONSIN
21 N PARK ST STE 6401
MADISON,WI 53715-1218
Performing Department
Department of Food Science
Non Technical Summary
Mass spectrometry is a tool that is increasingly used to address critical research needs on food and agriculture. This technology can be used to discover and measure molecules present in foods, microbes, or tissue samples. Highly specialized instrumentation is needed to increase the specificity and capacity of research on food and health. The objective of this application is to obtain a new ultra-high performance liquid chromatography - mass spectrometer (UHPLC-MS/MS) to enable research focused on food and health. The proposed instrument will be for shared-use by investigators aligned with this research area and housed at the Department of Food Science in the College of Agriculture and Life Sciences at the University of Wisconsin-Madison. The requested instrument is a UHPLC equipped with an Orbitrap Exploris 240. This specific instrument will enable research that requires highly sensitive, specific, and quantitative analyses of molecules related to critical needs in advancing food production, safety, and health. The identified users of the instruments are pursuing research and research training that are closely aligned with regional and national priorities in agriculture. These include: metabolism and bioavailability of health-promoting food components; mechanisms by which probiotic bacteria elicit their health promoting effects; impact of diet on gut microbiota in the context of chronic disease prevention; pathogenesis of Listeria monocytogenes, a prominent foodborne pathogen in humans and originating from dairy cattle; novel methods to improve food structures and monitor food quality; improving dairy and meat quality; improving fermentation processes for producing novel health-promoting dairy products; and bioconversion of lignocelluloic biomass, whey, and food wastes to value-added biochemicals and biofuels. The results from this newly-enabled research will be disseminated to the food industry and public via a robust outreach network which includes UW Extension, Dairy Innovation Hub, Food Research Institute, UW Frozen Desserts Center, and Center for Dairy Research.
Animal Health Component
25%
Research Effort Categories
Basic
50%
Applied
25%
Developmental
25%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
7011499101010%
7013430100010%
7013450101010%
7021129101010%
7011549101010%
7023450109010%
7234010110010%
7021121109010%
5011419100010%
5114010110010%
Knowledge Area
511 - New and Improved Non-Food Products and Processes; 702 - Requirements and Function of Nutrients and Other Food Components; 501 - New and Improved Food Processing Technologies; 723 - Hazards to Human Health and Safety; 701 - Nutrient Composition of Food;

Subject Of Investigation
1499 - Vegetables, general/other; 3430 - Cheese; 1419 - Leguminous vegetables, general/other; 1549 - Wheat, general/other; 1129 - Berries and cane fruits, general/other; 1121 - Cranberry; 4010 - Bacteria; 3450 - Milk;

Field Of Science
1090 - Immunology; 1010 - Nutrition and metabolism; 1100 - Bacteriology; 1000 - Biochemistry and biophysics;
Goals / Objectives
The overall objective of this application is to obtain a new ultra-high performance liquid chromatography - mass spectrometer (UHPLC-MS/MS) to enable research focused on food and health.This specific instrument will enable research that requires highly sensitive, specific, and quantitative analyses of molecules related to critical needs in advancing food production, safety, and health.The specific objectives are as follows: 1) The instrument will be acquired, delivered, and installed at Babcock Hall in the Department of Food Science at the University of Wisconsin-Madison. After this, 2) the instrument will be operational and users will be trained. 3) We will establish user access and management, data sharing/processing methods, and begin to collect user fees. 4) The instrument will be maintained and user fees will be collected to contribute to service costs. 5) In years 2 & 3, we review the financial model for instrument use. 6) Data and publications are anticipated based on instrument use.The results from this newly-enabled research will be disseminated to the food industry and public via a robust outreach network which includes UW Extension, Dairy Innovation Hub, Food Research Institute, UW Frozen Desserts Center, and Center for Dairy Research.
Project Methods
The UHPLC-MS system will be composed of the Thermo Scientific Orbitrap Exploris 240 benchtop mass spectrometer with a UHPLC front end. The UHPLC will consist of a binary pump, solvent degasser, temperature-controlled autosampler, column oven, and diode-array detector. The system will be in-line with the mass spectrometer. The instrument will be controlled by computer with equipped with software for automated operation of the UHPLC and mass spectrometer. The XCalibur software for data analysis will be installed and used for compound identification and quantification.The shared-use UHPLC-MS system will facilitate the following applications: A) discovery and quantification of molecules; B) small molecule characterization (e.g. metabolites from foods, microbes, reaction products, peptides); C) protein characterization (e.g. from foods, microbes, tissue samples); D) "-omics" approaches (lipidomics, proteomics, untargeted metabolomics) applied to foods and other biological systems; E) high resolution and rapid application of MS/MS methods via UHPLC.The instrument will be established for shared use and supported and maintained by a sustainable funding model. We have a core group of users aligned with local, regional, and national agricultural priorities in the area of food production and food and health. Additional users from the institution, industry, regional institutions are anticipated through the Food Research Institute, the UW Dairy Innovation Hub; Center for Dairy Research (Industry); and listing in the core lab/shared instrument database at UW-Madison. Additional marketing will be provided by effort from the Food Science IT staff.We will track training events, instrument use (e.g,. # of samples), number of users, data, and communications resulting from this instrument to adequately determine the impact of this project on the intended audience. We anticipate analysis of 180 samples in year 1, and 404 samples in years 2 and beyond, which will be key criteria for success. We expect our users to include faculty (14), senior staff (14), postdocs (8), graduate students (37), and undergraduates (16) working in food quality and food and health.

Progress 07/01/21 to 06/30/23

Outputs
Target Audience:The target audiences for this project were (1) potential users of the UHPLC-MS/MS system, which included individuals working on food and health research and industry collaborators; and (2) students or trainees interested in mass spectrometry for food and health research. This included high school students visiting campus, undergraduate students, graduate students, and postdoctoral fellows. Our efforts included: User outreach: We explained instrument capabilities and the potential for uses by advertising on our departmental website, explanation at faculty meetings, and held one-on-one meetings with interested individuals. Tours of lab and overview of instrument and uses: Interested users met with Dr. Bolling (PI), Dr. Rankin (co-PI), or laboratory personnel to view the instrument and analytical capabilities. At least three high school student groups toured the laboratory facility and had 10-15 min. overview of the capabilities and uses of the instrument. Posters and outreach at research conferences: Research methods were presented at scientific and industry conferences (e.g. UW Dairy Innovation Hub; Food Research Institute; Center for Dairy Research conferences). Posters were presented illustrating instrument capacity. Incorporation into instruction. Analytical content and data-based case studies were developed for a new graduate-level course in Food Science, FS711, Food Biochemistry. The course was offered for the first time in Fall 2023. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The project enabled training activities on instrument use. This included installation training, a two-day hands-on instrument training workshop provided by the instrument manufacturer, a follow-up training seminar by the instrument manufacturer, and new user training by laboratory personnel. It also allowed one-on-one training of postdoctoral fellows, graduate students, and faculty by working directly with trained instrument users. The project enabled professional development of graduate students. Students conducting research on the instrument have developed presentations at research conferences and preparation of research theses. Furthermore, the instrument has been incorporated into an annual graduate-level course in food science, which will enable ~ 20 students per year to gain skills in data analysis and interpretation by processing and interpreting data in FS 711 (Food Biochemistry). How have the results been disseminated to communities of interest?The results of the project have been communicated through communications at scientific research conferences, direct outreach and reports to interested users (e.g. food companies or other researchers on campus), incorporation into instructional and outreach activities. Dr. Bolling has also reported instrument availability through the Food Research Institute and UW Dairy Innovation Hub. Within the last year, the Bolling lab hosted visiting groups of high school students and gave an overview of the instrument with an outreach event, "Why good food goes bad." What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Objective #1) The instrument will be acquired, delivered, and installed at Babcock Hall in the Department of Food Science at the University of Wisconsin-Madison. N/A: This objective was completed before this reporting period. Objective #2) The instrument will be operational, and users will be trained. The instrument was installed by the manufacturer and has been operational in the Department of Food Science at the University of Wisconsin-Madison since September 2022. Users have been trained by the manufacturer, Dr. Bolling, or laboratory personnel. At least seven users are fully trained to operate the instrument independently. A second tier of ~ 30 students have been trained in data processing and analysis. Thus, samples can be submitted or run by trained instrument users, and students trained in data analysis can then process data according to their experimental objectives. Objective #3) We will establish user access and management, data sharing/processing methods, and begin to collect user fees. Protocols for user access and management have been developed and posted internally to users and potential users. We have developed data sharing methods and have cloud-based and local storage for instrument data. User fees have been collected beginning in February 2023. Therefore, this objective has been achieved. Objective #4) The instrument will be maintained, and user fees will be collected to contribute to service costs. A fee structure has been established and approved by the University. User fees have been collected beginning in February 2023. User fees are placed into a fee-for-service account that will be applied to fund the service contract and personnel running the instrumentation. Therefore, this objective has been achieved. Objective #5) In years 2 & 3, we review the financial model for instrument use. Because of the delay in the installation and operation of the equipment, we have not collected a full year of user fees. We will review the financial model after a year of collecting user fees in February 2024. Therefore, this objective is in progress, but will be completed after the project expires. Objective #6) Data and publications are anticipated based on instrument use. The instrument has been put to use and research outputs are accumulating. These include data presented in research posters, preliminary data for proposals, and peer-reviewed publications. The topic areas have been aligned with the overall instrument goals. The data have focused on food quality, food composition, metabolites of molecules in foods, metabolomic analysis of gut microbiota and other microbial experiments, characterization of natural antimicrobials, and markers of food quality and health.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Lang D, Liu C, Bolling BW. Rapid, comprehensive high resolution mass spectrometry analysis of dairy products. UW Dairy Innovation Hub Symposium. Madison, WI. May 18, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Liu C, Wicks CJ, Hartel RW, Bolling BW. Polyphenol profiles of grape seed extracts and the effects of their physiochemical properties on microstructure formation in cream/ice cream systems. USDA NIFA 2023 Investigators Meeting UC-Davis, CA.
  • Type: Other Status: Published Year Published: 2023 Citation: Bolling B. Green beans and health. H.M. Clause. Sun Prairie, WI. April 19, 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Choi, D (2023). Application of Food-Grade Fungi to Safeguard Global Food Safety. Oral presentation delivered at the Kyungpook National University, School of Food Science & Biotechnology, Korea. October 2023.
  • Type: Theses/Dissertations Status: Published Year Published: 2023 Citation: Choi, D (2023). Development, Characterization, and Application of Food-Grade Fungal Cultures for Global Food Safety. Oral presentation delivered at the Department of Food Science, Dissertation for Ph.D degree, USA. June 2023
  • Type: Other Status: Published Year Published: 2023 Citation: Choi, D (2023). Development and Applications of Novel Food-Grade Aflatoxin Detoxifier and Broad-Spectrum Antimicrobials using Aspergillus oryzae. Oral presentation delivered at the Department of Food Science, FS Seminar, USA. February 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Olson, E. G., D. K. Dittoe, C. Chatman, E. L. Majumder, and S. C. Ricke. 2023. Peptide structures on cecal microbiota inoculated with Campylobacter jejuni. International Association for Food Protection Annual. Meeting, Toronto, Ontario, July 2023.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Olson, E. G., D. K. Dittoe, C. Chatman, E. L. Majumder, and S. C. Ricke. 2023. Effect of general media and enrichment media on characterization of cecal microbiota inoculated with Campylobacetr jejuni. International Association for Food Protection, UW-Madison, June 2023
  • Type: Conference Papers and Presentations Status: Published Year Published: 2022 Citation: Poster Presentation: Chatman, C., et al. (2022). Co-exposure to polyethylene microplastics and Salmonella typhimurium in ceca of broiler chickens. American Society for Microbiology Microbe Conference, Washington, DC.?
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Poster Presentation: Chatman, C., et al. (2022). Co-exposure to polyethylene microplastics and Salmonella enterica Typhimurium in ceca of broiler chickens. KB Raper Symposium, Madison, WI.?
  • Type: Conference Papers and Presentations Status: Published Year Published: 2022 Citation: Poster Presentation: Chatman, C., et al. (2022). Co-exposure to polyethylene microplastics and Salmonella enterica Typhimurium in ceca of broiler chickens. Midwest Microbial Pathogenesis Conference, Madison, WI.?
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Poster Presentation: Chatman, C., et al. (2023). Effects of co-exposure to polyethylene microplastics and Salmonella enterica Typhimurium in ceca of broiler chickens. SciMed GRS Annual Poster Session, Madison, WI.?
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Oral Presentation: Chatman, C., et al. (2023). Effects of co-exposure to polyethylene microplastics and Salmonella enterica Typhimurium in ceca of broiler chickens. Emerging Contaminants in the Environment, Urbana, IL.??
  • Type: Conference Papers and Presentations Status: Published Year Published: 2023 Citation: Poster Presentation: Chatman, C., et al. (2023). Effects of co-exposure to polyethylene microplastics and Salmonella enterica Typhimurium in ceca of broiler chickens. Food Research Institute Spring Meeting, Madison, WI.?


Progress 07/01/21 to 06/30/22

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Students and postdoctoral fellows were trained on instrument protocols by the instrument manufacturer and the PI. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period, we expect to accomplish additional training activities, expand instrument access, and begin collection of user fees. Thus, we expect to make significant progress toward objectives 2-5 of the project, over the next reporting period.

Impacts
What was accomplished under these goals? High-resolution mass spectrometry is an excellent tool to characterize molecules highly relevant to food quality and health. Access to instrumentation is critical to advance research on food production, safety, and health. The Department of Food Science and its aligned programs requested funding to obtain a high-resolution mass spectrometer (UHPLC-MS/MS) instrument to advance research, training, and education in food and health. During the first year of this project, the UHPLC-MS/MS was purchased and installed at the Department of Food Science at the University of Wisconsin-Madison. Initial training activities were conducted to prepare the instrument for broader access and training opportunities. Therefore, we have accomplished objective #1 and enabled progress toward subsequent objectives.

Publications