Progress 05/01/24 to 04/30/25
Outputs
Target Audience:We reported part of the data as poster presentations at Stem Day at AAMU campus Changes/Problems:Due to the unavailability of the animal room at AAMU in the last two -three years, the PD and Co-PD had to change the methodologyto complete the part of the project about "Testing the Safety and Toxicity of Selenium Nanopartiles". We originally proposed to use mouseas an animal model to complete this goal, however, the building that houses the animal rooms was reconstructed and renovated in the last 2-3 years. The Co-PD changed the mouse model to a nematode, Caenorhabditis elegans (C. elegans), a well-established model organism for toxicological and oxidative stress studies. The new methodolgy, including design and methods,is described below: Experimental Design: Model organisms, wild-type N-2 strain Caenorhabditis elegans will be exposed to selected concentrations of Se-NPs for a duration on 24, 48 and 72 hours, and the negative control group will include untreated worms. Worms will be synchronized to ensure consistency. Se-NPs will be added to Nematode Growth Medium (NGM) plates seeded with bacterial strain Escherichia coli - OP50 and C. elegans will be exposed to Se-NPs for the short (24-hrs) and long-term (72-hrs) periods under controlled temperature (20oC). For motility and behavioral assays, Body Bends and Thrashing assays will be conducted to determine body bends per minute to assess movement impairment and swimming behavior in liquid medium, respectively. Wild-type nematode, C. elegans, eggs through L4-stage will be harvested using standard protocol on Nematode Growth Medium agar plates seeded with bacterial strain E. coli - OP50. C. elegans will be evaluated for toxicity after exposure to a range of Se-NP [C] based on prior literature reporting. EC50 and LD50 will be used to select low, medium and high Se-NP [C]. The determined low and medium [C] will be used to examine progeny and brood size of C. elegans. Due to this change in methodolgy, we did not renew theIACUCplan for this project What opportunities for training and professional development has the project provided?During this period, the funding of this project supported two graduate students and one undergraduate student. The student made poster presentation at our on-campus stem day conference and won 2nd place in poster section. How have the results been disseminated to communities of interest?We reported the results in the research community on campus since this part of the project has not been completed. The on-going research will further test the mechanisms that the C. elegans use to respond to SeNPs treatment. These preliminary data provide a foundation for the investigators to further seek funds to support the future research. What do you plan to do during the next reporting period to accomplish the goals?The project ends in April 2025. We do not plan to do further research for this project. However, the on-going research will further test the mechanisms that the C. elegans use to respond to SeNPs treatment and the benefits of SeNP to comsumers if it is incorporated in food. These preliminary data provide a foundation for the investigators to further seek funds to support the future research.
Impacts
What was accomplished under these goals?
During this period, we testedthe toxicity, reproductive outcomes, and antioxidant potential of Se NPs in the nematode, Caenorhabditis elegans (C. elegans), a well-established model organism for toxicological and oxidative stress studies. In addition, the antioxidant activity of Selenium nanoparticles were examined. C. elegans were exposed to varying concentrations of Se NPs (0.001 - 0.1 μg/mL) to assess acute toxicity, reproductive outcomes, and antioxidant potentials of Se NPs compared to control models with no exposure to nanoparticles. Reproductive outcomes were assessed by quantifying total progeny, egg-laying rates, and developmental abnormalities. Results indicate that while low concentrations of Se NPs exhibit negligible toxicity, organisms exposed to Se NPs at 0.001 and 0.01 μg/mL, respectively, show a decrease in progeny, decrease development with a halt at L2 stage, and give rise to aggregation of worms encased in a biofilm matrix. Worms exposed to Se NPs at 0.1 μg/mL exhibit a 2-fold increase reproductive success in progeny with multigenerational development. These findings contribute to understanding the dose-dependent biological effects of Se NPs and their implications for nanomedicine and environmental safety. DPPH assayindicated that SeNPs alone, may have the ability to inhibit the DPPH radical, and in a biological system, possibly increase other Aox pathways. This may indicate and elucidate additional Aox properties of SeNPs.
Publications
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Progress 05/01/21 to 04/30/25
Outputs
Target Audience:The results of the project were report on ARD symposium in April 2022, on AAMU campus stem day 2022 and published in jounral "Microorganisms" in June 2023. The targeted audience include researchers, scientistis who work on the research related to food preservation, food prosessing and a broader range of audience of scientifi journal who are interested in antimicrobial effects of nanoparticles. In addition, the participnats at ARD symposiumincluded policy makers and stakeholders in the larger 1809 land-grant community. Changes/Problems:Due to the unavailability of the animal room at AAMU in the last two -three years, the PD and Co-PD had to change the methodology to complete the part of the project about "Testing the Safety and Toxicity of Selenium Nanopartiles". We originally proposed to use mouse as an animal model to complete this goal, however, the building that houses the animal rooms was reconstructed and renovated in the last 2-3 years. The Co-PD changed the mouse model to a nematode, Caenorhabditis elegans (C. elegans), a well-established model organism for toxicological and oxidative stress studies. The new methodolgy, including design and methods, is described below: Experimental Design: Model organisms, wild-type N-2 strain Caenorhabditis elegans will be exposed to selected concentrations of Se-NPs for a duration on 24, 48 and 72 hours, and the negative control group will include untreated worms. Worms will be synchronized to ensure consistency. Se-NPs will be added to Nematode Growth Medium (NGM) plates seeded with bacterial strain Escherichia coli - OP50 and C. elegans will be exposed to Se-NPs for the short (24-hrs) and long-term (72-hrs) periods under controlled temperature (20oC). For motility and behavioral assays, Body Bends and Thrashing assays will be conducted to determine body bends per minute to assess movement impairment and swimming behavior in liquid medium, respectively. Wild-type nematode, C. elegans, eggs through L4-stage will be harvested using standard protocol on Nematode Growth Medium agar plates seeded with bacterial strain E. coli - OP50. C. elegans will be evaluated for toxicity after exposure to a range of Se-NP [C] based on prior literature reporting. EC50 and LD50 will be used to select low, medium and high Se-NP [C]. The determined low and medium [C] will be used to examine progeny and brood size of C. elegans. Due to this change in methodolgy, we did not renew the IACUC plan for this project What opportunities for training and professional development has the project provided?This project supported training 1 undergraduate student, two graduate students, one postdoctor fellow (half year). The experience the trianees gained was invaluble and landed a foundation for their success in career. How have the results been disseminated to communities of interest?Through symposium, conference, and publications, the results were disseminated to 1890 land-grant communities and larger group of anudience who are interested in antimicrobial activities of nanoparticles and food preservation. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
We completed the goal 1, 2, 3, and part of goal. We had to modified methodology of goal 4 since the animal room at AAMU was not availabe due to two rennovations during the award duration.
Publications
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Progress 05/01/23 to 04/30/24
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?A Master graduate student is working on this project forher thesis dissertation research. She has completed her proposal and will defend her proposal in the Spring of 2024. How have the results been disseminated to communities of interest?The results of the characterization of the selenium nanoparticles and investigation of antimicrobial effects of selenium nanoparticles against food-borne bacteria were published in journal : Microorganisms?2023 Jun 7;11(6):1519.,doi: 10.3390/microorganisms11061519. What do you plan to do during the next reporting period to accomplish the goals?We will continue to analyze the safety and toxicity of selenium nanoparticles using mammalian cell models and animal model. Due to a more than one-year rennovation of the building that houses the mammanlian cell culture room and animal room, this part has been delayed. We will need a no cost extension of the project to allow us to complete the remaining research proposed in this project.
Impacts
What was accomplished under these goals?
During May 2023-April 2024, we have completed the following tasks: 1) Characterized the chemically synthesized selenium nanoparticles 2) tested the antimicrobial effects of Selenium nanoparticles against 11 bacteria that can cause food-borne infections. 3) tested whether selenium nanoparticles can help preserve food quality
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Evaluation of Antibacterial Activity of Selenium Nanoparticles against Food-Borne Pathogens. Microorganisms
. 2023 Jun 7;11(6):1519. doi: 10.3390/microorganisms11061519.
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Progress 05/01/22 to 04/30/23
Outputs
Target Audience:We reported part of the data as poster presentation at ARD meeting in Altalanta Geogiain April of 2022. Changes/Problems:No major changes, due to the rennovation of the building that houses lab mice and rats during the period of fall 2021 to fall 2022, the safety test of selenium nanoparticles at animal level will be delayed and may need ask for a non-cost extenstion of the project What opportunities for training and professional development has the project provided?The funds of this project supported post-doctoral training for half years and now a M.S graduate is working on this project for her dissertation thesis. The funds also supported an undergraduate research assistant for a semester. He made a poster presentation of the data obtained last year. How have the results been disseminated to communities of interest?A manuscript is being prepared and will be submitted within a month. What do you plan to do during the next reporting period to accomplish the goals?We plan to test the safety of selenium nanoparticles in mammalian cells (epithelial cells) We plan to explore the possible mechanisms that selenium nanoparticles inibit bateral growth.
Impacts
What was accomplished under these goals?
During this period, we completed the first goal and the majority part of the second goal 1) successfully synthesized selenium nanoparticles that exbihibted desired properties for antimicrobial assay. 2) Tested the antioxidant activity of the selenium nanoparticles (showed antioxidant activity at high concentration) 3) tested antimicrobial activity of selenium nanoparticles against E. coli, S. auria, L monocytogenes, and other 8 more bacteria. 4) Evaluated the protective effects of selenium nanoparticles against spoilage
Publications
- Type:
Journal Articles
Status:
Other
Year Published:
2023
Citation:
N/A
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Progress 05/01/21 to 04/30/22
Outputs
Target Audience:
Nothing Reported
Changes/Problems:The major change in this period is the change of method of nanoparticle synthesis. In the proposal, we planned to use bacterial synthesis of nanoparticles to produce selenium nanoparticles. The method produced nanoparticles in high yield and with the suitable physical properties for antimicrobial asaay. However, we encountered difficulty in isolating the nanoparticles from cellular debris. After test and trial, we chose a vitamin C-mediated reduction of sodium selenite into selenium nanoparticles. The vitamin C- mediated method produce clean nanoparticles with high yield and optimal size and morphology that are very suitbale for antimicrobial assay. What opportunities for training and professional development has the project provided?The project provided support and training for apostdoctoral fellow. In addition, an undergraduate student who wassupported by univeristy scholarship received training in bacterial culture and antimicrobial assay How have the results been disseminated to communities of interest?The results will be reported in Stem Day at AAMU in March, and ARD meeting in April in Atlanta, GA. What do you plan to do during the next reporting period to accomplish the goals?We will start to test the antimicrobial activity of the selenium nanoparticles and whether the selenium nanoparticles can stabilize food quality in the next year.
Impacts
What was accomplished under these goals?
Tested conditions for biological selenium nanoparticle synthesis, however, we realized that the selenium nanoparticles produced using this platform were not ideal for testing antimicrobial activity, due to the difficulty in isolating nanoparticles from cellular debris. Therefore, we optimized the method using vitamin C to reduce sodium selenite and successfully produce selenium nanoparticles for the antimicrobial assay. We characterized the physical properties of selenium nanoparticles. The results suggested that the nanoparticles are suitable for antimicrobial activity assay
Publications
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