Source: WASHINGTON STATE UNIVERSITY submitted to NRP
IMPROVING CROP RESILIENCY: MULTIDISCIPLINARY TRAINING OF FUTURE AGRICULTURE LEADERS THROUGH RESEARCH AND EXTENSION
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1026080
Grant No.
2021-68018-34617
Cumulative Award Amt.
$499,962.00
Proposal No.
2020-09365
Multistate No.
(N/A)
Project Start Date
Apr 1, 2021
Project End Date
Mar 31, 2026
Grant Year
2021
Program Code
[A7401]- Research and Extension Experiences for Undergraduates
Recipient Organization
WASHINGTON STATE UNIVERSITY
240 FRENCH ADMINISTRATION BLDG
PULLMAN,WA 99164-0001
Performing Department
Institute Biological Chemistry
Non Technical Summary
This proposal aims to develop a self-sustaining training program in research and Extension with a focus on crop resiliency to heat and drought. The main objectives are: (i) create an experiential learning curriculum for undergraduates that integrates crop biology with Extension; (ii) provide training in critical thinking, problem solving, digital competency, and communication skills; (iii) increase participation of underrepresented and economically disadvantaged students in crop research and Extension; (iv) promote careers in crop sciences and Extension by educating undergraduates about different career paths and by helping them to establish professional contacts with peers, mentors, and stakeholders; (v) generate new knowledge on crop adaptation mechanisms to heat and drought; and (vi) strengthen the partnership between Washington State University and neighboring PUIs Lewis-Clark State College, Eastern Washington University, and Gonzaga University for training undergraduates in crop research and Extension. The main training element of the program is a summer internship. A total of ten interns will be recruited from the participating institutions. Each intern will rotate between two laboratories to learn the methodologies of both foundational and applied disciplines in crop sciences including biochemistry, genetics, molecular biology, and physiology. Extension specialists will engage students in field trials and organize workshops, farm visits, and meetings with the regional commodity commissions. Collecting and analyzing data from the laboratory and field trials will provide training in digital competency. Preparing posters and online educational materials about their work and presenting these materials to each other, mentors, and stakeholders will facilitate development of communication and leadership skills.
Animal Health Component
50%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2061599102020%
2031599106040%
2031899100040%
Goals / Objectives
Our overall goal is to develop a mentored undergraduate training program that immerses students in integrated research and Extension projects on plant resiliency to heat and drought stress, helps with career orientation, and prepares them for further training. Program activities will be designed and governed by a group of experienced mentors from the WSU Institute of Biological Chemistry (IBC), Department of Crop and Soil Sciences (CSS), and Extension who have expertise in varied plant science disciplines, extensive knowledge of stakeholder groups, and a considerable history of undergraduate training. The program will attract students from underrepresented and economically disadvantaged groups through collaboration with three PUIs within the PNW (Figure 1), as well as WSU Native American Programs, with WSU acting as the central hub. Our recruiting will focus on first-year and sophomore students, as they are most likely to be deciding the direction of their future careers. The educational activities are structured to address the following six objectives:Objective #1. Create an experiential learning curriculum for undergraduates that integrates multidisciplinary training in crop biochemistry, molecular biology, physiology, and breeding with Extension. The educational activities will be based on locally important crops including winter and spring pulse crops, winter and spring wheat, winter and spring canola, spring barley, and sunflowers; however, the skills gained by the students will have wide applicability to all crops.Objective #2. Provide training in critical thinking, problem solving, digital competency, and communication skills needed for enrollment in further education stages and/or employment.Objective #3. Advance education equality by increasing participation of underrepresented and economically disadvantaged students in crop research and Extension.Objective #4. Promote careers in crop sciences and Extension sciences by educating undergraduates about different career paths in academia, Extension, and industry and by helping them to establish professional contacts with peers, mentors, and stakeholders.Objective #5. Generate knowledge on the adaptation mechanisms to heat and drought in different cropping systems.Objective #6. Strengthen the partnership between WSU and neighboring PUIs for training undergraduates in crop research and Extension.
Project Methods
Recruitment and selection of interns will be undertaken in four stages: advertising the program; introducing potential applicants to research activities in the mentors' laboratories; collecting and evaluating the applications; and extending offers for participation. We anticipate recruiting 1-2 students from each participating organization per year (total of 10). Strong applicants that cannot be offered a place in one year's roster will be considered in the following year.Research projects. Integrated research and Extension projects will address diverse aspects of crop response and resiliency to heat and drought. Students will gain both research and Extension skills through close interactions with mentors. The Garland-Campbell and Lange laboratories will collaborate to investigate drought tolerance in spring wheat by generating breeding populations in Garland-Campbell's lab that will be analyzed for the accumulation of drought-induced metabolites in Lange's lab (5-13). The focus of these investigations will be on osmolytes, which are small molecules--such as proline, sucrose, raffinose and malic acid--that play key roles in maintaining cell volume and fluid balance under drought stress. The resiliency of promising genotypes will be tested in collaboration with mentor Tao on farms located across various climate zones and soil types. The Steber and Browse laboratories will collaborate with mentor Van Vleet on the analysis of hormonal response to stress in ongoing varietal trials of pulses. The aim of this work is to identify varieties with better performance under heat and drought. Physiological measurements, such as stomatal conductance and photosynthesis activities in Steber's lab, will be combined with measurements of synthesis of the stress hormone jasmonate in Browse's lab. The Sanguinet, Smertenko, and Bates laboratories will collaborate with VanVleet to identify optimal root architecture and response to drought and heat stress in pulses. Root architecture analysis in Sanguinet's lab will be correlated with biochemical responses to drought such as reactive oxygen species [ROS] production and activity of ROS scavengers in Smertenko's lab and with accumulation of lipids in Bates' lab. Mentors Sullivan, Peters, Bartley and Tao will host students to work on a collaborative project assessing the effects of microbes that enhance soil-health (metal ion and nitrogen availability) on cereal and/or oil crop root and shoot growth and development. Students will interact with farmers, field agronomists, and other stakeholders by participating in integrated Research & Extension programs such as field days, webinars, and podcasts.Mentoring activities prior to the project start. Once a student accepts an offer, and prior to arriving on the Pullman campus, the PD will start to prepare them for the summer program. Students will receive information about housing and dining in the Pullman area and about travel arrangements. Mentors will send the interns a targeted reading list and other relevant information. The interns will have the opportunity to discuss the projects with their mentors at weekly intervals leading up to the program through email, videoconferencing, or a personal meeting. On a technical note: as relevant, seeds will be planted in March for the field trials.Training activities will be organized with the main goal of providing interns with the knowledge and skills that will facilitate their career orientation and enable them to enter relevant career development programs in the future. During the first two days, the interns will have tutorials on (i) environmental health and laboratory safety procedures; (ii) scientific background and project aims; (iii) experimental design; and (iv) research methods. Students will then be teamed in pairs based on common interests and the pair will work on the same project. Each student will receive identical training at the IBC, Dept. of CSS, and Extension. Training and experiments in each laboratory will be performed as part of a coherent project co-mentored by the Extension specialist. The time spent by students in each lab will depend on the project requirements and students' interests.All students will be trained in basic laboratory techniques such as pipetting, preparing solutions, and adjusting pH. Next, the students will learn more advanced methods as per the requirements of their respective research project. These methods could include, but are not limited to, extracting DNA, RNA, proteins, lipids, or other biological molecules; measuring plant stress response using chlorophyll fluorescence, stomatal conductance, or the greenness index; and operating PCR machines, spectrophotometers, electrophoresis instruments, and other scientific equipment. Students will develop digital competency by collecting and analyzing numerical data using software packages. As their technical expertise and confidence grow, the students will start working on the specific research project goals. All laboratory activities will be performed as collaborations between the mentors and graduate students or postdoctoral scientists in the corresponding laboratories who will aid interns in learning techniques as well as oversee working practices and adherence to laboratory safety procedures.Each student pair will discuss their training and research progress with the mentors once a week. The rationale for this activity is that positive interactions with senior scientific personnel contribute to students' research gains. Mentors will take turns to introduce the whole group of interns to their research program at a weekly seminar. Also, once per week the interns will have a 1 hour-long educational activity such as a career development brown bag session led by WSU faculty or a workshop on writing abstracts, designing scientific posters, etc. These activities will be organized and managed by program coordinator Dr. Matt Peck. Students will be encouraged to create LinkedIn accounts and establish a network between themselves and the mentors.One day per week will be dedicated to Extension activities. These will include workshops by Extension faculty on Extension skills, tools, and technologies including experiments or demonstration trials that are relevant to the interests of PNW growers and grower groups. Furthermore, students will visit farms to interact with farmers and learn about precision agriculture technologies and practices aimed at reducing the impact of drought on yield. They will also participate in Field Days and seminars where students, along with Extension faculty, will present research outcomes to growers and commodity groups.Assessing learning progress. Mentors will formatively assess the learning progress through daily observation of students in the lab and through personal meetings every week or sooner as necessitated by the project. The assessment criterion will be students' ability to answer specific questions about the project and about the training experience. Another formative assessment will be feedback from the graduate students or postdoctoral scientists who supervise the interns in the lab. The next assessment will be a final poster that summarizes the project. Electronic versions of the posters will be peer-reviewed by the interns and modified in line with the feedback. Hard-copy posters will then be presented at a symposium during the last week of the internships. Presenting in front of peers provides valuable experience in critical evaluation of their data and public speaking; furthermore, it provides strong motivation for a career in science. Each poster presentation will be judged by three mentors. The presentation will be assessed on the poster quality, ability to explain the project, and ability to adequately answer questions. The scores and feedback will be used as a summative assessment of the training.

Progress 04/01/23 to 03/31/24

Outputs
Target Audience:The target audience are undergraduate students, graduate students, post-doctoral scientists, faculty, famers, stakeholders, and public. Changes/Problems:The first challenge was the increase of the housing price. The WSU Undergraduate Research Office arranged accommodation in a dormitory with air conditioning in anticipation of heat waves. As a consequence, the price of accommodation increased from $1,100 to $2,100 per student for the entire program. We accommodated this price increase by reducing the allowance for training materials from $1,000 per student to $600 per student, and by using the money budgeted for travel. The second challenge is that four members of the program left WSU over the last year. One of the extension specialists, Dr. Tao, was offered a job in another institution. Dr. Van Vleet took responsibility for the extension activities in the summer of 2022. In Fall of 2022 Dr VanVleet retired from WSU. His role was taken over by two extension specialists. Dr. Rachel Wieme, an extension specialist in Walla Walla County and Carol McFarland, a coordinator of Farmers Network took on design management of the Extension activities. What opportunities for training and professional development has the project provided?Undergraduate students were offeres opportunities to learn about agricultural research, expension, and science communication. Post-doctoral and gradueate students contributed to mentoring of students that was an essential experience for their professional development. Specific detail on the training activitei are provided below. Research projects The following projects were conducted by the students: Mentors: Tarah Sullivan & Michael Pumphrey Project title: Microbial Remediation of Acidic Soils under wheat production Description:This project focuses on understanding microbial mechanisms in the rhizosphere for buffering soil acidity and carbonate production in areas of the Palouse where soil acidification has become a major limiting factor for yield. Working with the spring wheat breeding program, we are testing the above-ground production and vigor of various wheat genotypes, as well as the specific microbial community they recruit for increased buffering of soil acidity and aluminum toxicity.The depth and complexity of the project will be adjusted according to each individual's background and experience. Experience and skills: Organization and management of field trials, analysis of basic soil health properties (physical and chemical), soil genomic DNA extraction, bacterial and fungal cultivation and aseptic technique, selective cultivation, shotgun and amplicon sequencing, sequence analysis for individual isolates as well as bioinformatics of rhizosphere microbial communities. Mentors: Phil Bates, Karen Sanguinet, Andrei Smertenko Project title: Sustaining oil production in hot and dry climates Description:This project focuses on understanding the mechanism of crop resiliency to heat and drought with a particular emphasis on understanding and engineering increased production of plant oils used for food, bio-fuels, or chemicals. You will spend time in each lab learning techniques and will contribute to generation and analysis of material collected in field trials, greenhouses and growth chambers. The depth and complexity of the project will be adjusted according each individuals background and experience. Experience and skills: Organization and management of field trials, analysis of seed development using microscopy, genome editing using CRISPR-Cas9 technology, extraction of lipids, proteins and DNA, gas chromatography, spectrophotometry, statistical analysis, and data presentation. Mentors: Mark Lange and Andrei Smertenko Project title: Role of GABA in heat and drought stress of spring wheat Description:This project will investigate production of GABA (gamma-aminobutyric acid) response to heat and drought stress. The first step will be general training in sample preparation, analytics, data analysis, and how to generate reproducible data. Second, optimize the method for separation and detection of GABA. Third, obtain a calibration curve. Fourth, measure GABA production in leaves of one spring wheat genotype subjected to drought stress in the greenhouse settings. Experience and skills: growing plants in the greenhouse, sampling field trials, extraction of metabolites, chemical processing of metabolites to prepare for downstream analysis; operation of gas chromatography-mass spectrometry equipment; metabolomics data analysis. Mentors: Laura Bartley and Kimberly Garland-Campbell. Project title: An introduction to laboratory and field techniques in Biofortification Biofortification: The process of increasing the concentration and availability of mineral nutrients and vitamins in crops for human consumption. Problem: The goal of this project is to establish the variation in concentration of phytate in wheat germplasm using a high-performance liquid chromatography. Results will be used to breed wheat for improved human nutrition. Improved human nutrition can increase quality of life and reduce the need for dietary meat, facilitating vegetarian diets, which have a lower climate-impact relative to omnivorous diets. Experience and skills: Perform extraction of phytate from samples of wheat flour; General training on chromatographic separation and quantification of phytate; Occasional field trips to hand harvest samples grown in the field to minimize contamination from the elements of interest found in nature and to measure other plant traits; Analyze, visualize and communicate regarding project data. Trainers: Camille Steber, Rob Brueggeman Project title: Preventing problems from preharvest germination of wheat and barley. Description:This project focuses on the mechanisms controlling alpha-amylase protein expression during germination of barley and wheat grain.The student will spend time in the field (Spillman Farm) contributing to wheat and barley collection for greenhouse germination tests and spend time in the lab learning techniques for the analysis of the alpha-amylase enzymes produced during germination that degrade starch. The depth and complexity of the project will be adjusted according to each individual's interest, background, and experience. Experience and skills: Organization and management of field trials, analysis of seed development and germination, comparison of alpha-amylase gene sequences in barley and wheat, extraction of proteins, western analysis, enzyme assays, interaction with farmers, statistical analysis, and data presentation. Presentation of posters at the symposium Students were taught and mentored on the preparation of posters summarizing their laboratory projects. There was a choice to team up with another student or make an individual poster. Posters were reviewed by mentors and students used feedback to improve the quality of posters. Students were also given an opportunity to present the poster at mentor lab meetings and get feedback from the research group. The symposium was held on August 4, 2023. The posters were mounted in the Center for Undergraduate Education (CUE) building on WSU Pullman campus. Our program recruited judges to evaluate design, materials, and presentation. Each poster was assigned three judges. Posters with the highest scores were offered prizes. The top prize was a cash prize of $200. Two runners up were awarded cash prizes of $100. The winning poster was by Skyler Allison from Washington State University, the poster by Oscar Rodriguez and Nallely Leon won the second prize, and two posters shared the third place: Dana Passinhas-Bergman's and Trinity Hanning's poster, and Anna Carroll's poster. Evaluation of program We used a Qualtrics-deployed survey developed by Dr. Peck to anonymously acquire quantitative and qualitative feedback from participants. We asked trainees to score their gains in professional skills, experience, and knowledge from participating in four components of the program: (i) working in the lab; (ii) working on the extension project; (iii) working on symposium poster; and (iv) interacting informally with others in the lab and the program. The list below shows the mean gain from ten responses on a scale from 0 - "None at all" to 4 - "A great deal." Gain reported by trainees for four main activities Activity Mean Gain, 4 point scale Working in lab 3.70 Working on the extension project 3.70 Working on the symposium poster 3.80 Interacting informally with others in the lab and the program3.60 Analysis of open-ended qualitative feedback identified one area for improvement: 1. Improve coordination between the Extension and laboratory-based activities to separate, in particular have deadlines for the extension project several weeks before the symposium to allow more time on completion of research projects. How have the results been disseminated to communities of interest?1. Interns studied published materials on soil health and communicated results of their search as a presentation to farmers and stakeholders at Field day on the WSU Wilke research farm. 2. Web-blog "Drought Resilience in Dry Land: Plant Auxins and Adaptive Management" by Anna Buetow, Carol McFarland, and Rachel Wieme was published on WSU web site. https://csanr.wsu.edu/drought-resilience-in-dry-land-plant-auxins-and-adaptive-management/ 3. Carol McFarland and Oscar Rodriguez recorded a podcast with Moses Boone about field scale pH survey at a Holland-Boone Farm. The discussion features co-production of research around a featuring the research and extension experiences for undergraduate students and the use of in-field pH meters and experimental design to capture pH variability across the field and within the soil profile and a discussion of low pH symptoms in lentils, nitrogen fertilizer contribution to low pH, and the benefits of the co-production of research. This podcast was downloaded 72 times. https://www.pnwfarmersnetwork.org/podcast/on-farm-trials-ft-moses-boone-and-oscar-rodriguez/ 4. Skyler Allison, Nallely Leon, Rachel Weime, and Carol McFarland published a web blog "What's weighing down your soil?" on the Washington Soil Health Initiative website. https://washingtonsoilhealthinitiative.com/2023/09/whats-weighing-down-your-soil/ 5. Eli Eleccion and Heidi Vandyk recorded a Wheat Beat podcast "Covering the bases of soil acidity and liming with". https://soundcloud.com/wsu-wheat-beat-podcast/wbp164?utm_source=clipboard&utm_medium=text&utm_campaign=social_sharing 6. Anna Carroll and Anna Buetow recorded a Wheat Beat podcast "Different aspects of drought resilience". https://soundcloud.com/wsu-wheat-beat-podcast/wbp166?utm_source=clipboard&utm_medium=text&utm_campaign=social_sharing What do you plan to do during the next reporting period to accomplish the goals?1. Maintain recruitment activities across PUIs in Washington State. Provide more specific information about program activities and clearly describe duties and responsibilities of the students during recruiting. 2. Maintain successful practices from the first year in conducting activities that aim at development at team building, research training, extension experience, and communication skills. 2. Learn from and aim to complete all extension activities by the end of the first week of July. 3. Run the "ice-breaking" session before the program start and weekly meetings during the program. Organize at least two social activates for our REEU in addition to the activities organized by the Undergraduate Research Office 4. Keep research projects focused and well-designed. 5. Include production of podcasts in the extension component of the program.

Impacts
What was accomplished under these goals? During the third year of the funding cycle our efforts focused on optimizing all key stages of the program taking into account feedback from the students and partners. The feedback was collected at the end of the program using an open-ended Qualtrics survey. The feedback identified the following areas for improvement: 1. Students requested more onboarding activities before the start of the program projects that introduce information about the projects and allow trainees to become better acquainted with each other and with mentors. 2. More specific and focused low-risk project design. 3. Expectations about the content and format of research projects were not met for some students. Specific activities, procedures, and outcomes are described below. Recruitment In the first stage of the recruitment Dr. Laura Bartley gave a research seminar at Gonzaga University that contained information about the summer program. The program was advertised at Eastern Washington University, Gonzaga University, Lewis-Clark State College, Whitman College, and Central Washington University using flyers that contained a QR code for the Qualtrics application form. In addition the program was advertised on the WSU Undergraduate Research Office Website. The form was developed by Dr Peck in year 2 and includes questions about the student's background, previous experience, motivation for joining the program, and expectations from the program. Applicants were selected for their interests and background for the interviews by Dr Peck and Dr Smertenko. The interviews were conducted over a 30 minute Zoom-meeting. One of the discussion points during the interview was students' interests and preference for a type of laboratory project. Each student was given an opportunity to select a project on the whole-organism or molecular level. Then student accepted the offer and their names and affiliations are listed below: The 2023 season participants that accepted the offer Student Home institution Anna Carroll Eastern Washington University Yizhen Zhao Whitman College, Walla Walla Anna Buetow Whitman College, Walla Walla Heidi Vandyk Western Washington University Nallely Leon Lewis Clark State College Dana Passinhas-Bergman Washington State University Skyler Allison Washington State University Emilio Eleccion Washington State University Oscar Rordiguez Washington State University Trinity Hanning Washington State University This group includes students from diverse cultural and socioeconomic backgrounds: 6 from underprivileged socioeconomic backgrounds (italics), three from diverse cultural background, 8 females, one male, and one transgender. A student was considered from an underprivileged socioeconomic background if they identified themselves as "Have/had no parents or legal guardians that completed a bachelor's degree (i.e., you are a first generation college student). Were or currently are eligible for Federal Pell grants. Received support from the Special Supplemental Nutrition Program for Women, Infants and Children (WIC) as a parent or child. Were eligible for the Federal Free and Reduced Lunch Program for two or more years". Preparation and execution of projects. Two "ice-breaker" sessions were organized during May to get students acquainted with each other. Mentors met with students by Zoom in May to discuss the project objectives and provide students with relevant literature. We accommodated different start dates for students who are on semesters and quarters (Eastern Washington University and Western Washington University). Students worked individually or in groups of two or three on five projects described below. Faculty, post-doctoral scientists, and graduate students in the corresponding labs contributed to training students. In addition to the lab projects, students participated in weekly meetings run by Dr. Peck and Dr. Smertenko. The meetings focused on keeping cohesion in the group and mitigating issues that students could experience in all aspects of the program starting from housing and ending with the mentorship. The topics for the meetings are listed below: May 30, 2023 Orientation and rules of working in a research lab, informing about the goals of the program July 7, 2023. Sharing lab experiences, discussing science communication skills, and writing abstracts about the intern's projects. July 14, 2023. Picnic in the Hells Canyon. July 202023. Providing feedback on student's abstracts and developing the final version of the abstract for submitting to the WSU Undergraduate Research Office. Discuss preparation of posters. July 27, 2023. Troubleshooting making posters, checking that students get feedback on the poster from their lab mentors. August 4, 2023. Symposium for the summer research interns across WSU. 2.2. Extension project All trainees participated in the extension project "On-farm experimentation for improving soil health ". This project was mentored by WSU Whitman County Extension Specialist Dr. Rachel Wieme and Dr. Carol McFarland. Description:Soil nutrient uptake and accumulation is greatly influenced by soil pH. Throughout most of the Pacific Northwest (PNW), soil health and soil pH has significantly degraded over the past century. This project provides experience with the soil acidity pH, approaches for measuring soil pH on the research farms and at farmers fields, and learning about solutions for improving soil health. Experience and skills: knowledge about soil acidity and approaches to improve soil health, general knowledge about farm organization, communication of science to the public. Activities of the extension project are listed below. Students were divided in three groups and each picked one of the following projects: (i) Soil acidity and health; (ii) On-farm experimentation; (iii) mitigating the impact of stress on plants. Students were trained to do presentations through the following activities: (i) practice presentations in the class and get feedback from mentor and peers, (ii) observed presentations by faculty and extension specialists during Lind Field Day, and (iii) analyzed experience from the field day and adjusted their presentations. The presentation was at a field day at Wilke research farm. After presenting, students answered questions from the audience, which comprised local farmers and agricultural technology company vendors, providing trainees with an opportunity to engage with end users of WSU research. Other activities included recording three podcasts, and writing two web blogs. To support the Extension-related activities we conducted weekly meetings on the topics indicated below. June 1, 2023. Introduction to topics and three paths for presenting the extension projects: field day presentation, podcasts, and web blog. June 8, 2023. Creating a detailed draft plan for extension presentations. June 15, 2023. Trip to the Washington State University Lind research farm Field Day to observe the techniques used for communicating relevant topics to stakeholders by faculty and extension specialists. June 22, 2023. Reflection on the observations from the Lind Field Day and refine the plan for the presentation at the Field Day at WSU Wilke research farm. June 28, 2023. Rehearsal of the presentation at the Wilke Field Day. June 29, 2023. Presentation at the Wilke Field day. July 25, Recording a podcast and deadline to submit a draft of the web blogs.

Publications


    Progress 04/01/22 to 03/31/23

    Outputs
    Target Audience:Target audiences were undergraduate students and and farmers. Changes/Problems:The first challenge was the increase of the housing price. The WSU Undergraduate Research Office arranged accommodation in a dormitory with air conditioning in anticipation of heat waves. As a consequence, the price of accommodation increased from $1,100 to $1,600 per student for the entire program. We accommodated this price increase by reducing the allowance for training materials from $1,000 per student to $600 per student. The second challenge is that four members of the program left WSU over the last year. One of the extension specialists, Dr. Tao, was offered a job in another institution. Dr. Van Vleet took responsibility for the extension activities in the summer of 2022. In Fall of 2022 Dr VanVleet retired from WSU. His role was taken over by two extension specialists. Dr. Rachel Wieme, an extension specialist in Walla Walla County and Carol McFarland, a coordinator of Farmers Network. Carol started the Farmers Network project together with Dr. Tao and will be able to expose students to the Farmers Network activities. What opportunities for training and professional development has the project provided?Students participated in the following training and professional development activities: Research projects Project 1. Mentors: Phil Bates, Karen Sanguinet Project title: Sustaining oil production in hot and dry climates Description: This project focuses on understanding mechanism of crop resiliency to heat and drought with a particular emphasis on production of oils. Trainees will spend time in each lab learning techniques and will contribute to generation and analysis of material collected in field trials, greenhouses and growth chambers. The depth and complexity of the project will be adjusted according each individual's background and experience. Experience and skills: Organization and management of field trials, analysis of seed development using microscopy, genome editing using CRISPR-Cas9 technology, extraction of lipids, proteins and DNA, gas chromatography, spectrophotometry, statistical analysis, and data presentation. Project 2. Mentors: Kim Garland-Campbell, Mark Lange Project title: Phenotypic and metabolic adaptations correlating with drought tolerance in wheat Description: This project will investigate breeding populations of wheat, members of which differ in their tolerance to drought treatment, for phenotypic variation and the differential induction of small molecules (metabolites). The focus of these investigations will be on using drone technology to survey field-grown wheat and on the accumulation of osmolytes, which are small molecules--such as proline, sucrose, raffinose and malic acid--that play key roles in maintaining cell volume and fluid balance under drought stress. Experience and skills: drone flights and image acquisition; harvesting of field-grown plant material; extraction of metabolites; chemical processing of metabolites to prepare for downstream analysis; operation of gas chromatography-mass spectrometry equipment; image and metabolomics data analysis. Project 3. Mentors: Camille Steber, Andrei Smertenko, Steve VanVleet Project title: Preventing problems from preharvest germination of wheat. Description: This project focuses on the mechanisms controlling alpha-amylase protein expression during germination of wheat grain. Wheat can initiate germination on the mother plant if there is rain before harvest, leading to problems with poor grain quality and financial losses for farmers. You will meet farmers, spend time in the field contributing to collection of wheat for greenhouse germination tests, and spend time in the lab learning techniques for the analysis of enzymes produced during germination that degrade wheat starch. The depth and complexity of the project will be adjusted according to each individual's interest, background, and experience. Experience and skills: Organization and management of field trials, analysis of seed development and germination, genome editing using CRISPR-Cas9 technology, extraction of proteins, western analysis, enzyme assays, extension and interaction with farmers, statistical analysis, and data presentation. Project 4. Mentors: Tarah Sullivan, John Peters, Laura Bartley, Steve VanVleet Project title: Soil-Root-Microbe Interactions: Focus on Nitrogen and Soil pH in Washington State Description: Attaining mineral nutrients and avoiding toxic metals is critical for plant health and crop yields, which are necessary to meet demand from a growing human population with a shifting diet. This project will examine the interaction of plants, bacteria and other microbes, and soil properties in greenhouse "mesocosm" studies that allow observation of root structures. Soil mixtures will include locally sourced substrates with varying pH. Microbial inoculants will include "diazotroph" microbes being developed as nitrogen biofertilizers. Plant species of focus are several spring wheat varieties with contrasting tolerance to pH stress, and switchgrass, a promising bioenergy crop. This project will generate new understanding of how plants and microbial communities interact with each other to adapt, and potentially modify, soil characteristics. Experience and skills: collection of field samples, microbiology, general laboratory techniques, statistical analysis and data presentation. Project 5. Mentors: Andrei Smertenko Title: Plant genetics and biochemistry Description: Plant growth and reproduction requires generation of new cells through cell divisions. Unlike animals that divide cells using constriction, plants partition daughter cells by the cell plate. Cell division is highly sensitive to heat stress. Increasing resiliency of this process to high temperatures. Our team studies impacts of heat on key stages of cell division. This project focuses on functional characterization of proteins responsible for regulation of microtubules in the phragmoplast. Experience and skills: plant sampling in field trails, DNA isolation and electrophoresis, analysis of gel images. Extension project All trainees participated in the extension project "Agronomic Responses to Acidic Soils". This project was mentored by WSU Whitman County Extension Specialist Dr. Steve Van Vleet. Description: Soil nutrient uptake and accumulation is greatly influenced by soil pH. Throughout most of the Pacific Northwest (PNW), soil health and soil pH has significantly degraded over the past century. This project focused on laboratory analysis of soil pH, soil nutrient levels via soil testing results, and the evaluation of crop roots in varying pH soils on production farms throughout eastern Washington. Students traveled to diversified farms with varying levels of soil acidity, collecting soil samples, evaluating soil pH and conducting presentation(s) at field day(s) on soil acidity and ways to mitigate further soil acidification. Experience and skills: knowledge about soil acidity and approaches to improve soil health, general knowledge about farm organization, communication of science to public. Activities of the extension project are listed in Table 2. Each student in the group picked a topic about soil health, collected information on this topic, and prepared a 5-minute long presentation. Students were trained to do presentations through the following activities: (i) practice presentations in the class and get feedback from mentor and peers, (ii) observed presentations by faculty and extension specialists at Spillman Farm, and (iii) analyzed experience from the field day and adjusted their presentations. The final presentation was at a field day in Dayton. After presenting, students answered questions from the audience, which was comprised of local farmers and agricultural technology company vendors, providing trainees with an opportunity to engage with end users of WSU research. Table 2. Extension project activities Date Activity Name Description of activity June 21 Farm Trip #1 Collecting soil samples and Farm Tour June 23 Class 1 Investigation of data related to soil health June 28 Class 2 Identify subtopics for trainee presentations June 30 Farm Trip #2 Attend field day in WSU Spillman research farm and observe presentations given by faculty and extension specialists July 5 Class 3 Discuss the field day experience and work on the trainee presentation July 7 Class 4 Practice the field day presentation July 11 Farm Trip #3 Present at the field day in Dayton How have the results been disseminated to communities of interest?Interns studied soil health topics and communicated results of their search in a lecture format to farmers and stakeholders at Walla Walla field day on July 11. Students were also taught and mentored on the preparation of posters summarizing their laboratory projects. There was a choice to team up with another student or make an individual poster. Posters were reviewed by mentors and students used feedback to improve the quality of posters. Students were also given an opportunity to present the poster at mentor lab meetings and get feedback from the research group. The symposium was held on July 29. The posters were mounted in the Center for Undergraduate Education (CUE) building on WSU Pullman campus. Our program recruited judges to evaluate design, materials, and presentation. Each poster was assigned three judges, and presenters with highest scores were offered prizes. The top prize was a bursary to attend a professional conference of student's choice. Two runners up were awarded a cash prize. The winner, Grace Cooper from Gonzaga University, plans to attend American Society for Microbiology meeting 2023(June 15-19) in Houston, TX. What do you plan to do during the next reporting period to accomplish the goals?1. Maintain the successful practices from the first year in conducting activities that aim at development of research, extension, and communication skills. 2. Learn from feedback in year one and modify the program to address weaknesses identified by the participants to improve student's learning experience. Specifically we plan to address the following areas. 2.1. Build stronger relationships between participants before the beginning of the program and during the program. During recruitment, program leaders will meet individually with each applicant to align expectations and build a relationship. Before the program begins, we will introduce participants to each other through a Zoom meeting. Afterwards students will meet every two weeks by Zoom to discuss the extension project. During the summer we will host coffee hours every week to talk to students about their experience. 2.2. Improve laboratory experience of students to have multiple experimental milestones allowing the students to be more part of the hypothesis-experiment-analyze-learn cycle. 2.3. Provide more specific information about program activities and clearly describe duties and responsibilities of the students during recruiting. 3. Recruit from two additional PUI in Washington State - Whitman College and Central Washington University. 4. Include production of podcasts in the extension component of the program. Potential topics for podcasts will be soil pH, soil fertility, carbon sequestration, liming, and production of biofuels from crops.

    Impacts
    What was accomplished under these goals? During the first year of the funding cycle we developed procedures for the key stages in the program: recruitment, preparation of the projects, execution of the projects, evaluation of learning outcomes, and discussions with project partners to identify and implement improvements for the following year. These procedures and outcomes are described below. 1. Recruitment In the first stage of the recruitment Dr. Smertenko and Dr. Peck gave research seminars that contained information about the summer program at Eastern Washington University, Gonzaga University, and Lewis-Clark State College. At each of these institutions we advertised the program using flyers that contained a QR code for the Qualtrics application form. The form was developed by Dr Peck and includes questions about student's background, previous experience, motivation for joining the program, and expectations from the program. Applicants who registered online were invited to participate in a Zoom-meeting with mentors to discuss projects. Students learned about projects and were given an opportunity to choose their project. Then we made offers to ten undergraduates and eight students listed in Table 1 accepted the offer. Table 1: 2022 participants Student Home institution Carissa, L, Morrison Eastern Washington University Bethlehem Alemu Yohannes Eastern Washington University Brend'n Marshal Blankenship Washington State University Zachary J Cunningham Lewis Clark State College Thomas Conway Washington State University Grace Cooper Gonzaga University Madilyn Mae Sorenson Lewis Clark State College Daria Rebecca Winterer Washington State University This group includes students from diverse cultural and socioeconomic backgrounds: 5 females and two first-generation students. 2. Preparation and execution of projects. Mentors met with students by Zoom in May to discuss the project objectives and provide students with relevant literature. We accommodated different start dates for students who are on semesters and quarters. Students worked individually or in groups of two or three on five projects described below. Faculty, post-doctoral scientists, and graduate students in the corresponding labs contributed to training students. 3. Evaluation We used a Qualtrics-deployed survey developed by Dr. Peck to anonymously acquire quantitative and qualitative feedback from participants. We asked trainees to score their gains in professional skills, experience, and knowledge from participating in four components of the program: (i) working in the lab; (ii) working on the extension project; (iii) working on symposium poster; and (iv) interacting informally with others in the lab and the program. Table 3. Gain reported by trainees for four main activities Activity Mean Gain, 5 point scale Working in lab 3.22 Working on the extension project 4.00 Working on the symposium poster 3.56 Interacting informally with others in the lab and the program 3.22 Table 3 shows the mean gain from nine responses on a scale from 1 - "None at all" to 5 - "A great deal." Trainees reported that they gained "A lot" from the Extension experience and between "A moderate amount" and "A lot" for the remaining components. Analysis of open-ended qualitative feedback identified the following areas for improvement: 1. More onboarding activities before start of the program projects that introduce information about the projects and allow trainees to become better acquainted with each other and with mentors. 2. More specific project design. Several students wanted to work on specific low-risk projects. 3. Expectations about the content and format of research projects were not met for some students. In addition to the feedback from students, we collected feedback from members of our advisory committee: Ben Barstow (Washington Grain Commission), Julie Beckstead (Gonzaga), Wendy Shuttleworth (LCSC), and Becky Brown (EWU). The committee suggested approaches for improving cohort teambuilding and recruitment, and also provided insights into expanding topics and presentation formats for extension activities.

    Publications


      Progress 04/01/21 to 03/31/22

      Outputs
      Target Audience:Undergraduate students, farmers, general public Changes/Problems:The major challenge for the project was late arrival of funds - April 2021. It was too late to start recruitment as many students have already found placements for the summer. Also it was challenging for the trainers to organize the projects at such short notice. We deiced to delay the start until 2022 season to allow time for preparing and conducting a successful recruitment program. What opportunities for training and professional development has the project provided?Project Coordinator Matthew Peck developed skills in making online surveys and currently learns the web page design. The dedicated webpage will be running by May 2022. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?Summer 2022 will be the first season for our program. During this time we will host undergraduates in research labs of the Institute of Biochemistry, Department of Crop and Soil Sciences, and WSU research farms. Students will learn new skills in the course of research and extension projects. Toward the end of the internship, the students will present their data at a mini-symposium. The quality of student's presentations and their knowledge will be assessed during the mini-symposium. This information along with reports will be provided to the advisory committee for evaluation and feedback. The feedback will be used to improve our practices in the recruitment and educational activities for the 2023 cycle.

      Impacts
      What was accomplished under these goals? Considering that funds arrived only in April 2021, it was too late to start recruitment for the 2021 summer activities. We decided to delay the first year of program until summer 2022. The recruitment efforts started in November 2021. We produced a flyer about the program and distributed it in the Washington State University, Gonzaga University, Eastern Washington University, and Lewis Clark State College using e-mail lists. In addition we gave seminars at Eastern Washington University on December 3 2021, in Gonzaga University on January 21, 2022, and in Lewis Clark State College on January 24, 2022. Program coordinator Dr. Matthew Peck produced an online application form. The closing date for the applications was January 31, 2022. We currently have 23 applicants: 8 from Lewis Clark State College, 3 from Gonzaga University, 7 from Eastern Washington University, and 5 from Washington State University. Of applicants who provided gender information, 9 are male and 11 are female. Four applicants are from the under-represented ethnic groups. Outcome of the recruitment activities indicate that we have attracted a diverse group of applicants. Next step, we will host applicants on campus or conduct a remote session by Zoom to introduce them to the trainers of the REEU program. The offers to the students will be made after these events. Students who can not be accommodated in the program will be offered places in other programs.

      Publications