Progress 01/15/24 to 01/14/25
Outputs
Target Audience:Concepts and results derived from our studies were used to illustrate classroom lectures in BIOL5350 (Mass Spectrometry Techniques for Metabolomics). Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The project provided the following opportunities for training and professional development in the Alonso lab at the University of North Texas: This project contributed to the training of one postdoctoral researcher (Dr. Julius Ver Sagun) and one graduate student (Jordan LaChance) who assisted with the mentoring of two undergraduate students. How have the results been disseminated to communities of interest?The results have been disseminated to communities of interest through 3 publications (2 research papers, and 1 book chapter) and 16 presentations (9 talks, and 7 posters): 1) A research article, "13C-labeling reveals non-conventional pathways providing carbon for hydroxy fatty acid synthesis in Physaria fendleri", published in Journal of Experimental Botany; 2) A research article, "First draft reference genome and annotation of the alternative oil species Physaria fendleri", published in G3; 3) A book chapter, "Efficient and powerful integration of targeted metabolomics and transcriptomics for analyzing the metabolism behind desirable traits in plants", published in Methods in Molecular Biology; 4) Ana Paula Alonso, Invited Speaker, "Multi-omics investigation into the synthesis of fatty acids in alternative crops" at the Interdisciplinary Plant Group Seminar Series, University of Missouri, Columbia, MO (March 4, 2024); 5) Julius ver Sagun, oral presentation, "An effective strategy for improving hydroxy- fatty acid production by Physaria fendleri" at the BioDiscovery Institute seminar series, Denton, TX (April 15, 2024); 6) Ana Paula Alonso, Invited Speaker, "Multi-omics Approach to Enhance Oil Production in Alternative Crops", at the Department of Biochemistry, Purdue University, West Lafayette, IN (April 16, 2024); 7) Ana Paula Alonso, Invited Speaker, "Mapping the Biosynthesis of Industrially Relevant Fatty Acids: Insights from 13C-labeling in Alternative Crops", at 2024 AOCS Annual Meeting and Expo, Montreal, Canada (April 28-May1, 2024); 8) Ana Paula Alonso, Invited Speaker, "Modeling and Analyzing Plant Metabolic Networks to Build the Crops of Tomorrow", at the University of Missouri, Columbia, MO (May 6, 2024); 9) Jordan LaChance, poster presenter, "Unraveling Metabolic Patterns in Developing Physaria fendleri Embryos, a Promising Alternative Oilseed Crop Rich in Hydroxy Fatty Acids" at the BioDiscovery Institute Research Expo 2024, Denton, TX (May 15, 2024); 10) Julius ver Sagun, poster presenter, "An effective strategy for improving hydroxy- fatty acid production by Physaria fendleri" at the BioDiscovery Institute Research Expo 2024, Denton, TX (May 15, 2024); 11) Julius ver Sagun, oral presentation, "An effective strategy for improving hydroxy- fatty acid production by Physaria fendleri" at the BioDiscovery Institute Research Expo 2024, Denton, TX (May 15, 2024); 12) Ana Paula Alonso, poster presenter, "Enhancing the Production of Industrially-Relevant Fatty Acids in Alternative Crops through a Multi-Omics Approach", at the Plant Biology 2024, Honolulu, HI (June 22-26); 13) Ana Paula Alonso, Invited Speaker, "Enhancing the Production of Industrially-Relevant Fatty Acids in Alternative Crops through a Multi-Omics Approach", at the Plant Biology 2024, Honolulu, HI (June 22-26); 14) Ana Paula Alonso, oral presentation, "Mapping the Pathways Leading to Industrially Relevant Fatty Acid Synthesis in Physaria fendleri", at the USDA NIFA 2024 AFRI Project Director's Meeting, Honolulu, HI (June 26-27, 2024); 15) Jordan LaChance, poster presenter, "Unraveling Metabolic Patterns in Developing Physaria fendleri Embryos, a Promising Alternative Oilseed Crop Rich in Hydroxy Fatty Acids", at International Symposium on Plant Lipids 2024, Lincoln, NE (July 14-19, 2024); 16) Yasmeen Elharis, poster presenter, "Lipid Analysis of Physaria fendleri for Improving Hydroxy Fatty Acid Production", at International Symposium on Plant Lipids 2024, Lincoln, NE (July 14-19, 2024); 17) Ana Paula Alonso, poster presenter, "Exploring Fatty Acid Synthesis in Alternative Crops via 13C-Labeling Approaches", at International Symposium on Plant Lipids 2024, Lincoln, NE (July 14-19, 2024); 18) Ana Paula Alonso, poster presenter, "Exploring Fatty Acid Synthesis in Alternative Crops via 13C-Labeling Approaches", at 19th GERLI International Lipidomics Meeting, Brest, France (September 23-26, 2024); and 19) Ana Paula Alonso, Plenary Speaker, "Comparative Omics Provide Insights into the Synthesis of Unusual Fatty Acids in Alternative Crops", at 19th GERLI International Lipidomics Meeting, Brest, France (September 23-26, 2024). What do you plan to do during the next reporting period to accomplish the goals?During the extension period, the Alonso lab at the University of North Texas anticipates to: 1) Complete 13C-metabolic flux analysis of developing HO embryos (by October 2025) 2) Finalize flux maps of HO and LO embryos (by January 2026) 3) Complete the bioinformatic analyses of the transcriptomic data for LO and HO embryos across five developmental stages (by June 2025) 4) Submission of at least one manuscript (by December 2025) 5) Mentoring undergraduate student(s) (over the entire extension period) 6) Presentation of results at conferences (over the entire extension period)
Impacts
What was accomplished under these goals?
The following tasks were accomplished in the Alonso lab at the University of North Texas to accomplish the project goals: 1) Draft mathematical model of the metabolic network in developing embryos from Physaria accession PI 596432 07i SD (low oil line, LO) using Isotopomer Network Compartmental Analysis; 2) Comparative lipidomics of mature embryos from two Physaria fendleri accessions PI 596432 07i SD (LO) and W6 20859 12i SD (high oil line, HO); 3) RNA extraction, quality check, and 30M/PE100 read sequencing (Innomics, Sunnyvale, CA) of HO and LO embryos across five stages of development in three biological replicates; 4) Preprocessing of RNAseq reads and transcriptome assembly for downstream analyses; 5) Training of a postdoctoral researcher (Dr. Julius Ver Sagun) to the techniques of mathematical modeling and lipidomics, a graduate student (Jordan LaChance) to the techniques of RNA extraction and RNAseq read analyses and two undergraduate students (Sanskriti Dwivedi, Brady Erlandsen).
Publications
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Cocuron J.C, Alonso A.P (2024) 13C-labeling reveals non-conventional pathways providing carbon for hydroxy fatty acid synthesis in Physaria fendleri. J Exp Bot. 75(6), 1754-1766.
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Johnston R.J, Horn P.J, Alonso A.P (2024) First draft reference genome and annotation of the alternative oil species Physaria fendleri. G3, 14(9), jkae114.
- Type:
Book Chapters
Status:
Published
Year Published:
2024
Citation:
Johnston R.J, Arias C.L, Alonso A.P (2024) Efficient and powerful integration of targeted metabolomics and transcriptomics for analyzing the metabolism behind desirable traits in plants. Methods in Molecular Biology. 2812, 47-99.
- Type:
Theses/Dissertations
Status:
Other
Year Published:
2024
Citation:
LaChance, J. (2024) Comparative Study of Biomass, Physiology, and Metabolome in High and Low Oil-producing Varieties of Physaria fendleri A Promising Hydroxy-fatty Acid Rich Oilseed Crop. MS Thesis, University of North Texas
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Ana Paula Alonso, Invited Speaker, Multi-omics investigation into the synthesis of fatty acids in alternative crops at the Interdisciplinary Plant Group Seminar Series, University of Missouri, Columbia, MO (March 4, 2024)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Julius ver Sagun, oral presentation, An effective strategy for improving hydroxy- fatty acid production by Physaria fendleri at the BioDiscovery Institute seminar series, Denton, TX (April 15, 2024)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Ana Paula Alonso, Invited Speaker, Multi-omics Approach to Enhance Oil Production in Alternative Crops , at the Department of Biochemistry, Purdue University, West Lafayette, IN (April 16, 2024)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Ana Paula Alonso, Invited Speaker, Mapping the Biosynthesis of Industrially Relevant Fatty Acids: Insights from 13C-labeling in Alternative Crops , at 2024 AOCS Annual Meeting and Expo, Montreal, Canada (April 28-May1, 2024)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Ana Paula Alonso, Invited Speaker, Modeling and Analyzing Plant Metabolic Networks to Build the Crops of Tomorrow , at the University of Missouri, Columbia, MO (May 6, 2024)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Jordan LaChance, poster presenter, Unraveling Metabolic Patterns in Developing Physaria fendleri Embryos, a Promising Alternative Oilseed Crop Rich in Hydroxy Fatty Acids at the BioDiscovery Institute Research Expo 2024, Denton, TX (May 15, 2024)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Julius ver Sagun, poster presenter, An effective strategy for improving hydroxy- fatty acid production by Physaria fendleri at the BioDiscovery Institute Research Expo 2024, Denton, TX (May 15, 2024)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Julius ver Sagun, oral presentation, An effective strategy for improving hydroxy- fatty acid production by Physaria fendleri at the BioDiscovery Institute Research Expo 2024, Denton, TX (May 15, 2024)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Ana Paula Alonso, poster presenter, Enhancing the Production of Industrially-Relevant Fatty Acids in Alternative Crops through a Multi-Omics Approach , at the Plant Biology 2024, Honolulu, HI (June 22-26)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Ana Paula Alonso, Invited Speaker, Enhancing the Production of Industrially-Relevant Fatty Acids in Alternative Crops through a Multi-Omics Approach , at the Plant Biology 2024, Honolulu, HI (June 22-26)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Ana Paula Alonso, oral presentation, Mapping the Pathways Leading to Industrially Relevant Fatty Acid Synthesis in Physaria fendleri , at the USDA NIFA 2024 AFRI Project Directors Meeting, Honolulu, HI (June 26-27, 2024)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Jordan LaChance, poster presenter, Unraveling Metabolic Patterns in Developing Physaria fendleri Embryos, a Promising Alternative Oilseed Crop Rich in Hydroxy Fatty Acids , at International Symposium on Plant Lipids 2024, Lincoln, NE (July 14-19, 2024)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Yasmeen Elharis, poster presenter, Lipid Analysis of Physaria fendleri for Improving Hydroxy Fatty Acid Production , at International Symposium on Plant Lipids 2024, Lincoln, NE (July 14-19, 2024)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Ana Paula Alonso, poster presenter, Exploring Fatty Acid Synthesis in Alternative Crops via 13C-Labeling Approaches , at International Symposium on Plant Lipids 2024, Lincoln, NE (July 14-19, 2024)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Ana Paula Alonso, poster presenter, Exploring Fatty Acid Synthesis in Alternative Crops via 13C-Labeling Approaches , at 19th GERLI International Lipidomics Meeting, Brest, France (September 23-26, 2024)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2024
Citation:
Ana Paula Alonso, Plenary Speaker, Comparative Omics Provide Insights into the Synthesis of Unusual Fatty Acids in Alternative Crops , at 19th GERLI International Lipidomics Meeting, Brest, France (September 23-26, 2024)
|
Progress 01/15/23 to 01/14/24
Outputs
Target Audience:Concepts and results derived from our studies were used to illustrate classroom lectures in BIOL 3510 (Cell Biology). Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The project provided the following opportunities for training and professional development in the Alonso lab at the University of North Texas: 1) the PD uses this project to illustrate the ongoing research in alternative crops and plant lipids during her class offered in Fall 2023 (total 148 local participants, undergraduates; 2) this project contributed to the training of one postdoctoral researcher (Dr. Julius Ver Sagun) and one graduate student (Jordan LaChance) who assisted with the mentoring of one undergraduate student. How have the results been disseminated to communities of interest?The results have been disseminated to communities of interest through 3 publications (1 review, 1 editorial article, and 1 research paper) and 10 presentations (6 talks, and 4 posters): 1) One review article, "Progress in understanding and improving oil content and quality in seeds" published in Frontiers in Plant Science; 2) One editorial article "Editorial: Metabolic architecture of developing seeds and grains" published in Frontiers in Plant Sciences; 3) One research article "13C-labeling reveals non-conventional pathways providing carbon for hydroxy fatty acid synthesis in Physaria fendleri" published in Journal of Experimental Botany; 4) Alonso AP, Invited Speaker, "AWARE: Advancing Weeds as Alternative Renewable Energy", Institute of Biological Chemistry, Washington State University, Pullman, WA (January 19, 2023). 5) Julius Ver Sagun, poster presenter, "An effective strategy for improving hydroxy- fatty acid production by Physaria fendleri" at the 2023 Plant Lipids Gordon Research Seminar in Galveston, Texas (January 28 - 29, 2023); 6) Julius Ver Sagun, poster presenter, "An effective strategy for improving hydroxy fatty acid production by Physaria fendleri" at the 2023 Plant Lipids Gordon Research Conference in Galveston, Texas (January 29 - February 03, 2023); 7) Alonso AP, Speaker, "Using 13C to Map the Pathways Leading to Industrially Relevant Fatty Acids" at the 2023 Plant Lipids Gordon Research Conference in Galveston, Texas (January 29 - February 03, 2023); 8) Alonso AP, Invited Speaker, "AWARE: Advancing Weeds as Alternative Renewable Energy" at the Physiology & Molecular Plant Biology Seminar Series, University of Illinois, Urbana-Champaign, IL (February 15, 2023); 9) Jordan LaChance, poster presenter, "Comparative Metabolomics of Developing Embryos from Physaria fendleri, an Alternative Oilseed Crop Specializing in Hydroxy Fatty Acid Production" at the 2023 Biology Graduate Student Association Research Symposium at the University of North Texas in Denton, Texas (April 13-14, 2023); 10) Jordan LaChance, oral presentation, "Targeted Metabolomics of Developing Embryos from Physaria fendleri, An Alternative Oilseed Crop Specializing in Hydroxy-fatty Acid Production" at the Biodiscovery Institute of the University of North Texas (May 4, 2023); 11) Alonso AP, Speaker, "13C-Labeling Reveals Non-Conventional Pathways Involved in Fatty Acid Synthesis in Alternative Crops", at the Gordon Research Conference on Plant Metabolic Engineering, Barcelona, Spain (June 11-16, 2023); 12) Jordan LaChance, poster presenter, "Targeted Metabolomic Profiling of Developing Embryos from Physaria fendleri, a Promising Alternative Oilseed Crop Rich in Hydroxy Fatty Acids" at the Metabolomics 2023 in Niagara Falls, Canada (June 18-22, 2023); 13) Jordan LaChance, oral presentation, "Metabolic engineering of Physaria fendleri to improve hydroxy-fatty acid content" at the Biodiscovery Institute of the University of North Texas (October 2, 2023). What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period, the Alonso lab at the University of North Texas anticipates to complete: 1) establish culture conditions that mimic the development of HO P. fendleri embryos; 2) 13C-metabolic flux analysis of developing HO embryos; 3) Comparative flux maps of HO and LO embryos; 4) comparative transcriptomic analyses of LO and HO embryos across five developmental stages; 5) submission of at least four manuscripts; 6) mentoring undergraduate student(s); and 7) presentation of results at conferences.
Impacts
What was accomplished under these goals?
The following tasks were accomplished in the Alonso lab at the University of North Texas to accomplish the project goals: 1) Parallel 13C-labeling experiments of developing embryos of Physaria fendleri accessions PI 596432 07i SD (LO); 2) Determination of 13C-labeling in intracellular metabolites and biomass components by LC-MS/MS and GC-MS; 3) Draft mathematical model of the metabolic network in developing LO embryos; 4) Completion of embryo biomass analysis (protein and starch content) at five development stages in Physaria fendleri accessions LO and W6 20859 12i SD (HO); 5) Collection of developing HO and LO embryos across five developmental stages in three biological replicates for future transcriptomic analyses; 6) Training of a postdoctoral researcher (Dr. Julius Ver Sagun) to the techniques of 13C-flux metabolic analysis, a graduate student (Jordan LaChance) to the techniques of transcriptomics and plant transformation, and one undergraduate student (Sanskriti Dwivedi).
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Sagun J.V, Yadav U.P, Alonso A.P (2023) Progress in understanding and improving oil content and quality in seeds. Front. Plant Sci., 14:1116894
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Amir R, Mart�nez-Force Enrique, Shi Jianxin, Alonso A.P (2023) Editorial: Metabolic architecture of developing seeds and grains. Front. Plant Sci., 14:1258353
- Type:
Journal Articles
Status:
Submitted
Year Published:
2023
Citation:
Cocuron J.C, Alonso A.P (2023) 13C-labeling reveals non-conventional pathways providing carbon for hydroxy fatty acid synthesis in Physaria fendleri. J Exp Bot. erad343.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Alonso AP, Invited Speaker, AWARE: Advancing Weeds as Alternative Renewable Energy , Institute of Biological Chemistry, Washington State University, Pullman, WA (January 19, 2023)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Julius Ver Sagun, poster presenter, An effective strategy for improving hydroxy- fatty acid production by Physaria fendleri at the 2023 Plant Lipids Gordon Research Seminar in Galveston, Texas (January 28 29, 2023)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Julius Ver Sagun, poster presenter, An effective strategy for improving hydroxy fatty acid production by Physaria fendleri at the 2023 Plant Lipids Gordon Research Conference in Galveston, Texas (January 29 February 03, 2023)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Alonso AP, Speaker, Using 13C to Map the Pathways Leading to Industrially Relevant Fatty Acids at the 2023 Plant Lipids Gordon Research Conference in Galveston, Texas (January 29 February 03, 2023)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Alonso AP, Invited Speaker, AWARE: Advancing Weeds as Alternative Renewable Energy at the Physiology & Molecular Plant Biology Seminar Series, University of Illinois, Urbana-Champaign, IL (February 15, 2023)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Jordan LaChance, poster presenter, Comparative Metabolomics of Developing Embryos from Physaria fendleri, an Alternative Oilseed Crop Specializing in Hydroxy Fatty Acid Production at the 2023 Biology Graduate Student Association Research Symposium at the University of North Texas in Denton, Texas (April 13-14, 2023)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Jordan LaChance, oral presentation, Targeted Metabolomics of Developing Embryos from Physaria fendleri, An Alternative Oilseed Crop Specializing in Hydroxy-fatty Acid Production at the Biodiscovery Institute of the University of North Texas (May 4, 2023)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Alonso AP, Speaker, 13C-Labeling Reveals Non-Conventional Pathways Involved in Fatty Acid Synthesis in Alternative Crops , at the Gordon Research Conference on Plant Metabolic Engineering, Barcelona, Spain (June 11-16, 2023)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Jordan LaChance, poster presenter, Targeted Metabolomic Profiling of Developing Embryos from Physaria fendleri, a Promising Alternative Oilseed Crop Rich in Hydroxy Fatty Acids at the Metabolomics 2023 in Niagara Falls, Canada (June 18-22, 2023)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2023
Citation:
Jordan LaChance, oral presentation, Metabolic engineering of Physaria fendleri to improve hydroxy-fatty acid content at the Biodiscovery Institute of the University of North Texas (October 2, 2023)
|
Progress 01/15/22 to 01/14/23
Outputs
Target Audience:Concepts and results derived from our studies were used to illustrate classroom lectures in BIOL 3510 (Cell Biology). Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?1) the PD uses this project to illustrate the ongoing research in alternative crops and plant lipids during her class offered in Spring 2022 (total 234 local participants, undergraduates; 2) this project contributed to the training of one postdoctoral researcher (Dr. Julius Ver Sagun) and one graduate student (Jordan LaChance) who assisted with the mentoring of two undergraduate students. How have the results been disseminated to communities of interest?The results have been disseminated to communities of interest through 11 presentations (4 talks, and 7 posters): 1) Julius Ver Sagun, poster presenter, "An effective strategy for improving hydroxy- fatty acid production by Physaria fendleri" at the 2022 Plant Biology Conference in Portland, Oregon (July 9-13, 2022); 2) Jordan LaChance, poster presenter, "A Multi-Omics Approach for Improving Hydroxy-fatty Acid (HFA) Production byPhysaria fendleri" at the 2022 Biodiscovery Institute Retreat in Denton, Texas (February 27, 2022); 3) Jordan LaChance, poster presenter, "Metabolic Engineering ofPhysaria fendlerito Improve Hydroxy-fatty Acid Content" at the 2022 Meeting of Southern Section of the American Society of Plant Biologists in Birmingham, Alabama (Marth 25-27, 2022); 4) Jordan LaChance, poster presenter, "Genetic Engineering ofPhysaria fendlerito Improve Oil Content" at the 2022 Biology Graduate Student Association Research Symposium at the University of North Texas in Denton, Texas (April 11, 2022); 5) Jordan LaChance, poster presenter, "Investigating metabolism ofPhysaria fendlerito Improve Hydroxy-fatty Acid Content" at the 2022 Federation Research Symposium Online (April 20, 2022); 6) Jordan LaChance, poster presenter, "A Multi-Omics Approach for Improving Oil Production byPhysaria fendleri" at the 2022 Biodiscovery Institute Board Meeting in Denton, Texas (May 10, 2022); 7) Jordan LaChance, poster presenter, "A Biochemical and Metabolomic Study of mature leaves of Physaria fendleri" at the 2022 Plant Biology Conference in Portland, Oregon (July 9-13, 2022); 8) Jordan LaChance, oral presentation at the Biodiscovery Institute of the University of North Texas, "A multi-omics approach for improving hydroxy- fatty acid (HFA) production by Physaria fendleri" (February 26, 2022); 9) Jordan LaChance, oral presentation at the Biodiscovery Institute of the University of North Texas, "Genetic Engineering of Physaria fendleri to Improve Oil Content" (May 9, 2022); 10) Jordan LaChance, oral presentation at the Biodiscovery Institute of the University of North Texas, "Metabolic engineering of Physaria fendleri to improve hydroxy-fatty acid content" (September 26, 2022); 11) Ana Paula Alonso, keynote presentation, "Using 13C-labeling to identify the pathways involved in fatty acid synthesis in alternative crops" at the International Symposium in Plant Lipids in Grenoble, France (July 1-15, 2022). What do you plan to do during the next reporting period to accomplish the goals?1) establish culture conditions that mimic the development of HO P. fendleri embryos; 2) 13C-metabolic flux analysis of developing LO embryos; 3) comparative transcriptomic analyses of LO and HO embryos across five developmental stages; 4) mentoring at least one undergraduate student; 5) presentation of results at conferences; 6) publication of a review on improving fatty acid content and composition in seeds; 7) publication of results.
Impacts
What was accomplished under these goals?
The following tasks were accomplished in the Alonso lab at the University of North Texas to accomplish the project goals: 1) development and validation of culture conditions for Physaria fendleri accessions PI 596432 07i SD (LO); 2) determination of the efficiency of which LO physaria embryos convert carbon into biomass components; 3) comparison of Physaria fendleri accessions PI 596432 07i SD (LO) and W6 20859 12i SD (HO) plant physiology and photosynthetic leaf activities; 4) determination of the biomass composition, fatty acid content and composition of LO and HO embryos across five developmental stages; 5) completion of targeted metabolomic analyses of LO and HO leaves; 5) targeted metabolomic analyses of LO and HO embryos across five developmental stages; 6) training of a postdoctoral researcher (Dr. Julius Ver Sagun) to the techniques of 13C-flux metabolic analysis, a graduate student (Jordan LaChance) to the techniques of plant transformation, and two undergraduate students (Matthew Wallace and Sanskriti Dwivedi).
Publications
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2022
Citation:
Julius Ver Sagun, poster presenter, An effective strategy for improving hydroxy- fatty acid production by Physaria fendleri at the 2022 Plant Biology Conference in Portland, Oregon (July 9-13, 2022)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2022
Citation:
Jordan LaChance, poster presenter, A Multi-Omics Approach for Improving Hydroxy-fatty Acid (HFA) Production by Physaria fendleri at the 2022 Biodiscovery Institute Retreat in Denton, Texas (February 27, 2022)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2022
Citation:
Jordan LaChance, poster presenter, Metabolic Engineering of Physaria fendleri to Improve Hydroxy-fatty Acid Content at the 2022 Meeting of Southern Section of the American Society of Plant Biologists in Birmingham, Alabama (Marth 25-27, 2022)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2022
Citation:
Jordan LaChance, poster presenter, Genetic Engineering of Physaria fendleri to Improve Oil Content at the 2022 Biology Graduate Student Association Research Symposium at the University of North Texas in Denton, Texas (April 11, 2022)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2022
Citation:
Jordan LaChance, oral presentation at the Biodiscovery Institute of the University of North Texas, Metabolic engineering of Physaria fendleri to improve hydroxy-fatty acid content (September 26, 2022)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2022
Citation:
Ana Paula Alonso, keynote presentation, Using 13C-labeling to identify the pathways involved in fatty acid synthesis in alternative crops at the International Symposium in Plant Lipids in Grenoble, France (July 1-15, 2022)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2022
Citation:
Jordan LaChance, poster presenter, Investigating metabolism of Physaria fendleri to Improve Hydroxy-fatty Acid Content at the 2022 Federation Research Symposium Online (April 20, 2022)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2022
Citation:
Jordan LaChance, poster presenter, A Multi-Omics Approach for Improving Oil Production by Physaria fendleri at the 2022 Biodiscovery Institute Board Meeting in Denton, Texas (May 10, 2022)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2022
Citation:
Jordan LaChance, poster presenter, A Biochemical and Metabolomic Study of mature leaves of Physaria fendleri at the 2022 Plant Biology Conference in Portland, Oregon (July 9-13, 2022)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2022
Citation:
Jordan LaChance, oral presentation at the Biodiscovery Institute of the University of North Texas, A multi-omics approach for improving hydroxy- fatty acid (HFA) production by Physaria fendleri (February 26, 2022)
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2022
Citation:
Jordan LaChance, oral presentation at the Biodiscovery Institute of the University of North Texas, Genetic Engineering of Physaria fendleri to Improve Oil Content (May 9, 2022)
|