Progress 01/15/24 to 01/14/25
Outputs
Target Audience:The USDA Fellows, specializing in the field of Food Science with an emphasis on Enhancing Gut Health, are educated through Purdue University's Food Science Graduate Program. This specialized curriculum trains them in specific areas such as (i) the impact of food bioactives and whole foods on gut physiology, (ii) the relationships between bioactive foods, food components, and gut microbiome structure and function, and (iii) food bioactive-regulated gut immunity/pathology. This approach bridges the gap between traditional food science and nutrition disciplines. The program's focus on the food matrix, coupled with exposure to fundamental research and industrial experiences in the current Foods for Health signature research specialization, elevates the training of these graduate students to a unique level not typically found in a Food Science program. These new USDA fellows are poised to bring a unique perspective and significant value to the food industry, government, and/or academia. Our program, which includes coursework, basic and applied research, and practical experiences, is designed to produce outstanding graduates. These individuals will possess the knowledge necessary to meet the critical needs of the food industry, government, and academia in the area of Foods for Health, with a particular focus on Food Science and Gut Health. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The three NNFs actively engaged in the Food Science Graduate Student Association at Purdue University in 2024. Moreover, Kayla Roy obtained a professional development opportunity from PepsiCo as a summer intern. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?The project promotes research activities to enhance public understanding and interest in food for gut health through fellows' active engagement in multiple areas: academic publications, research presentations, teaching, undergraduate student mentoring, and industry collaboration. Tongkewn Yoo was supported by this National Needs Fellowship (NNF) project from 2021 to 2024, with his fellowship concluding on December 31, 2024. Vignesh Nathan and Kayla Roy will continue to receive NNF support in the upcoming reporting period.
Impacts
What was accomplished under these goals?
VIGNESH NATHAN Graduate Research Progress: Completed preliminary exam Completed analysis of proof-of-concept piglet polyphenol supplementation study including short-chain fatty acid composition, fecal microbiome, and distal colon proteomics. Analysis to be completed (manuscript in preparation) Conducted a 5-week study utilizing blueberry polyphenols and a lactobacillus probiotic prophylactically to determine ameliorative effects in a DSS-induced colitis mouse model. Analysis completed (inflammatory markers, tight junction proteins, antioxidant enzymes, gut permeability, short-chain fatty acids) Analysis to be completed (bile acids/microbiome, manuscript in preparation) Currently conducting a 9-week study utilizing blueberry polyphenols and a bioengineered lactobacillus probiotic to determine restorative effects in vitro and in vivo utilizing a piroxicam induced colitis model in IL-10 KO mice. Analysis completed (in vitro: Growth curve, DPPH antioxidant activity, total phenolics, cell adhesion) (in vivo: 3 mice per treatment group have been completed with tissues harvested) Analysis to be completed (remaining 6 mice per treatment group, bile acids/microbiome/SCFA, pro-inflammatory markers, antioxidant enzymes, manuscript in preparation) Publications: Nathan, V.B.; Eckrote, S.; Li, S.; Reddivari, L. Crude Blueberry Phenolic Extracts Improve Gut Barrier Integrity and Exert Anti-Inflammatory and Antimicrobial Activity in an In Vitro Weaning Stress Model.Antioxidants2024,13, 1044. https://doi.org/10.3390/antiox13091044 Jiang, B.; Quinn-Bohmann, N.; Diener, C.; Nathan, V.; Han-Hallett, Y.; Reddivari, L.; Gibbons, S. M.; Baloni, P. (2024).Understanding disease-associated metabolic changes in human colon epithelial cells using iColonEpithelium metabolic reconstruction. Purdue University Research Repository.doi:10.4231/FD22-9D96 Conference Presentations: Nathan, V (2024). Effect of Blueberry Polyphenols on Improving Intestinal Health of Weaning Piglets. Institute of Food Technologists Expo, Chicago, IL Effect of Blueberry Polyphenol Supplementation in Improving Gut Health in Weaning Stressed Piglets. Food Science Industrial Associates Meeting, Purdue University, West Lafayette, IN Freeze-dried Blueberry Powder as a Dietary Supplement During Swine Weaning. Whistler Center for Carbohydrate Research Annual Poster Session, Purdue University, West Lafayette, IN KAYLA ROY Courses taken: STAT514 Food Science Seminar Graduate Research Progress: Analyzed VDR expression and activity in 7 different mouse studies of both DSS-induced colitis and spontaneous colitis from microbial colonization of IL-10-/- mice Conducted correlation analysis between microbiome and VDR expression and activity Prepared a manuscript from the results of this study Characterized dietary fibers based on their viscosities under gastric stimulated conditions Conducted microbial analysis to compare the influence of housing location on bacterial colonization of mice Publications: Wenyi F. Roy K. Cross T. Hamaker B. Li S. Reddivari L. "Anthocyanin-pectin complex ameliorates DSS-induced colitis". In preparation. Roy K. Moncada E. Reddivari L. "Ulcerative Colitis but not DSS induced colitis associated microbiota promotes early biomarkers of colitis in IL-10 -/- mice". Submitted, under review. Roy K. Reddivari L. "Colonic Inflammation, Microbiome, and High Fiber diets influence colonic VDR expression and activity in mouse models of colitis" In Preparation Conference Presentations: Roy K. Moncada E. Reddivari L. "Ulcerative Colitis promotes mucus thinning and colonic inflammation in IL-10 -/- mice". Industrial Associates. Marriot Hall, Purdue University. April 11, 2024. Roy K. Moncada E. Reddivari L. "Ulcerative Colitis promotes mucus thinning and colonic inflammation in IL-10 -/- mice". Whistler Center Annual Conference. Nelson Hall, Purdue University. April 24, 2024. Professional Development: Internship at PepsiCo TONGKEWN YOO Coursework-related: NUTR506 Biochemistry & Physiology I FS454 Food Chemistry Teaching assistant Graduate Research Progress: Renewed CITI certifications and indirect calorimeter training. Conducted research on the protection effect of piceatannol in a cancer cachexia model. Completed: in vitro study: myotube staining, western blot in vivo study: daily measurements (body weight, food intake, and tumor volume), Echo MRI, plasma analysis, and tissue analysis via western blot and H&E staining. In progress: preparing paper submission Conducted in vivo study utilizing parsnip extract as a feed additive with C57BL/6 mice bearing non-alcoholic-steatohepatitis (NASH) induced by high fat diet. Completed: daily measurements (body weight and food intake), Echo MRI, indirect calorimeter, and tissues and blood collection In progress: qPCR, western blot, histology analysis. Conducted in vivo study to investigate the anti-obesity effect of an acyl-CoA cholesterol acyl transferase inhibitor in relation to semaglutide treatment. Completed: daily measurements (body weight and food intake), Echo MRI, tissues and blood collection. In progress: qPCR, western blot, histology analysis. Conducted in vivo study feeding transformed yeast producing 3-hydroxybutyrate (3-HB) to see the change in gut microbiome and the anti-obesity effect of transformed yeast-derived 3HB. Completed: tissues and blood collection, LC-MS to measure 3-HB in blood and feces. Conducted in vitro study on the effect of piceatannol in D-galactose-induced senescence model. Completed: in vitro study: myotube staining Planned: in vivo mice study Poster Presentations: Yoo TK, Kim KH. Piceatannol Ameliorates Cancer-associated Muscle and Adipose Wasting, and Its Related STAT3 Activation. Purdue Institute of Cancer Research Cancer Research Day. 2024. Yoo TK, Ju JH, Kim JK, Kim KH. A Protective Effect of Aged Parsnip Extract on High-fat, High-sucrose, and High-cholesterol Diet-induced Hepatic Dysfunction in vivo. American Society of Nutrition Conference. Chicago, IL. 2024. Yoo TK, Kim KH. Piceatannol ameliorates myotube atrophy via STAT3 inhibition in an in vitro model of cancer-associated cachexia. Purdue Office of Interdisciplinary Graduate Programs Conference. 2024. Yoo TK, Kershaw J, Kim KH. Piceatannol ameliorates myotube atrophy via STAT3 inhibition in an in vitro model of cancer-associated cachexia. Purdue Food Science Student Research Poster Presentations & Industrial Associates Meeting. 2024. Yoo TK, Kershaw J, Kim KH. Piceatannol ameliorates myotube atrophy in an in vitro model of cancer-associated cachexia. Purdue College of Agriculture Network Gathering. 2024.
Publications
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
Piceatannol ameliorates myotube atrophy via STAT3 inhibition in an in vitro model of cancer-associated cachexia. Purdue Office of Interdisciplinary Graduate Programs Conference. 2024.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
Piceatannol Ameliorates Cancer-associated Muscle and Adipose Wasting, and Its Related STAT3 Activation. Purdue Institute of Cancer Research Cancer Research Day. 2024.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2024
Citation:
A Protective Effect of Aged Parsnip Extract on High-fat, High-sucrose, and High-cholesterol Diet-induced Hepatic Dysfunction in vivo. American Society of Nutrition Conference. Chicago, IL. 2024.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
Piceatannol ameliorates myotube atrophy via STAT3 inhibition in an in vitro model of cancer-associated cachexia. Purdue Food Science Student Research Poster Presentations & Industrial Associates Meeting. 2024.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
Piceatannol ameliorates myotube atrophy in an in vitro model of cancer-associated cachexia. Purdue College of Agriculture Network Gathering. 2024.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
Ulcerative Colitis promotes mucus thinning and colonic inflammation in IL-10 -/- mice. Industrial Associates. Marriot Hall, Purdue University. April 11, 2024.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
Ulcerative Colitis promotes mucus thinning and colonic inflammation in IL-10 -/- mice. Whistler Center Annual Conference. Nelson Hall, Purdue University. April 24, 2024.
- Type:
Other Journal Articles
Status:
Submitted
Year Published:
2024
Citation:
Ulcerative Colitis but not DSS induced colitis associated microbiota promotes early biomarkers of colitis in IL-10 -/- mice.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2024
Citation:
Effect of Blueberry Polyphenols on Improving Intestinal Health of Weaning Piglets. Institute of Food Technologists Expo, Chicago, IL
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
Effect of Blueberry Polyphenol Supplementation in Improving Gut Health in Weaning Stressed Piglets. Food Science Industrial Associates Meeting, Purdue University, West Lafayette, IN
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2024
Citation:
Freeze-dried Blueberry Powder as a Dietary Supplement During Swine Weaning. Whistler Center for Carbohydrate Research Annual Poster Session, Purdue University, West Lafayette, IN
- Type:
Peer Reviewed Journal Articles
Status:
Published
Year Published:
2024
Citation:
Nathan, V.B.; Eckrote, S.; Li, S.; Reddivari, L. Crude Blueberry Phenolic Extracts Improve Gut Barrier Integrity and Exert Anti-Inflammatory and Antimicrobial Activity in an In Vitro Weaning Stress Model. Antioxidants 2024, 13, 1044.
- Type:
Other Journal Articles
Status:
Published
Year Published:
2024
Citation:
Jiang, B.; Quinn-Bohmann, N.; Diener, C.; Nathan, V.; Han-Hallett, Y.; Reddivari, L.; Gibbons, S. M.; Baloni, P. (2024). Understanding disease-associated metabolic changes in human colon epithelial cells using iColonEpithelium metabolic reconstruction. Purdue University Research Repository.
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Progress 01/15/23 to 01/14/24
Outputs
Target Audience:The USDA Fellows, specializing in the field of Food Science with an emphasis on Enhancing Gut Health, are educated through Purdue University's Food Science Graduate Program. This specialized curriculum trains them in specific areas such as (i) the impact of food bioactives and whole foods on gut physiology, (ii) the relationships between bioactive foods, food components, and gut microbiome structure and function, and (iii) food bioactive-regulated gut immunity/pathology. This approach bridges the gap between traditional food science and nutrition disciplines. The program's focus on the food matrix, coupled with exposure to fundamental research and industrial experiences in the current Foods for Health signature research specialization, elevates the training of these graduate students to a unique level not typically found in a Food Science program. These new USDA fellows are poised to bring a unique perspective and significant value to the food industry, government, and/or academia. Our program, which includes coursework, basic and applied research, and practical experiences, is designed to produce outstanding graduates. These individuals will possess the knowledge necessary to meet the critical needs of the food industry, government, and academia in the area of Foods for Health, with a particular focus on Food Science and Gut Health. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The three NNFs actively engaged in the Food Science Graduate Student Association at Purdue University in 2023. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?Promoting the research activities to enhance public understanding and increase interest in food for gut health. This will be through active engagement of the fellows in publication, research presentation, teaching and mentoring undergraduate students, and communication/collaboration with food industries.
Impacts
What was accomplished under these goals?
The goals accomplished by the three NNFs in 2023 are as follows. Kayla Roy: Courses Taken: Molecular Microbiome Analysis Food Science Seminar Graduate Research Progress: Completed an 8-week observational study assessing whether mouse colitis-associated bacteria will cause IL-10 knockout mice to exhibit early onset and increased colitis severity. Assessed fecal lipocalin-2 values, mucus-thickness, cytokine levels, and intestinal permeability between mice gavaged with dss-associated bacteria versus healthy-associated bacteria. Assessed taxonomical differences between mice gavaged with dss-associated bacteria and healthy-associated bacteria Prepared manuscript for human and mouse colitis study Vignesh Nathan: Courses Taken: Statistical Design of Experiments Journey Through the Digestive Tract Graduate Research Progress: Completed all graduate classwork necessary for degree completion Completed CITI certifications and anaerobic chamber training Conducted a three-week proof of concept study utilizing blueberry polyphenols as a feed additive with twenty early weaning stressed piglets Analysis completed (C-reactive protein ELISA, TBARS ELISA, 8-Isoprostane ELISA, PCR of pro-inflammatory markers and tight junction proteins) Analysis to be completed (Fecal metabolites profile, Western Blot, Histology) Conducted in vitro fermentation of inulin fiber and various probiotics from Mead Johnson Nutrition to determine which strain/combination yielded significant short-chain fatty acid production. Analysis to be completed (testing blends using human colon cells) Harvested human primary colon epithelial cells and transformed human colon epithelial cells to determine if different metabolites are secreted. Tongkewn Yoo: Courses Taken: Statistical Design of Experiments Adipocyte Biology Graduate Research Progress: Completed CITI certifications and indirect calorimeter training. In vitro study about the protection effect of piceatannol against cancer cachexia model on C2C12 murine myotubes. Completed: myotube image quantification, qPCR of muscle atrophy markers In progress: western blot, immunohistology Planned: piceatannol in vivo treatment on cancer cachexia bearing mice model Conducted in vivo study utilizing parsnip extract as a feed additive with C57BL/6 mice bearing non-alcoholic-steatohepatitis (NASH) induced by high fat diet. Completed: daily measurements (body weight and food intake), Echo MRI, indirect calorimeter, and tissues and blood collection In progress: qPCR, western blot, histology, transaminase level measurement. Analyzed in vitro effect of parsnip extract on lipid accumulation of HepG2 human hepatocarcinoma. Conducted in vivo study feeding transformed yeast producing 3-hydroxybutyrate (3-HB) to see the change in gut microbiome and the anti-obesity effect of transformed yeast-derived 3HB. Completed: tissues and blood collection In progress: LC-MS to measure 3-HB in blood and feces, qPCR, western blot, histology.
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Roy K. Moncada E. Reddivari L. The Onset and Severity of Colitis in IL-10 -/- Mice is not Dependent on Fecal Microbiota Transplantation from Ulcerative Colitis Patients . Chrons and Colitis Foundation January 2023.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Roy K. Moncada E. Reddivari L. Bacterial dysbiosis does not increase colitis severity or onset in IL 10 -/- mice. Whistler Center for Carbohydrates February 2023.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Roy K. Moncada E. Reddivari L. Fecal Microbiota Transplantation from an Ulcerative Colitis Patient promotes mucus thinning and colonic inflammation in IL-10-/- mice Midwest Microbiome Symposium May 2023.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Nathan, V (2023). Effect of Crude Blueberry Phenolic Extracts on Improving Gut Barrier Integrity and Inflammation In Vitro, Current Developments in Nutrition, Volume 7, Supplement 1, pg 206. Submitted to American Society of Nutrition Annual Meeting 2023
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2023
Citation:
Yoo TK, Kim KH. Piceatannol ameliorates muscle atrophy potentially through STAT3 inhibition in an in vitro model of cancer-associated cachexia. Big Ten Academic Alliance Lipid Symposium. University of Iowa, IA. 2023.
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Progress 01/15/22 to 01/14/23
Outputs
Target Audience:These USDA Fellows in the area of Food Science Approach to Enhancing Gut Health educated in Food Science Graduate Program of Purdue University with a specialized curriculum will be trained in specific areas of (i) food bioactive and the whole food on gut physiology, (ii) relationships among bioactive foods and food components, and gut microbiome structure and function, and (iii) food bioactive-regulated gut immunity/pathology that bridges the gap between traditional food science and nutrition disciplines. A focus on the food matrix and exposure to fundamental research and industrial experiences in the current Foods for Health signature research specialization will elevate the training of graduate students to a new unique level not typical of a Food Science program. These new USDA fellows will bring a unique perspective and true value to the food industry, government and academia. Moreover, the proposed program of coursework, basic and applied research and experiences should generate outstanding graduates who will have the knowledge to help meet the critical needs of the food industry, government, and academia in the area of Foods for Health with a focus on the Food Science and Gut Health. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The USDA fellows are actively engaged in the graduate student club, departmental graduate seminar, Food Science Industrial Associates, and courses related to the gut microbiome. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?Promoting the research activities for the purpose of enhancing public understanding and increasing interest in food for gut health. This will be through active engagement of the fellowsin publication, research presentation, teaching and mentoring undergraduate students, and communication/collaboration with food industries.
Impacts
What was accomplished under these goals?
Two additional USDA fellows havejust been recruited and joined the Food Science Graduate Program of Purdue Univerisityin the Spring and Fall of 2022. Now a total ofthree USDA fellows are now studying the role of dietary regulations of lipid metabolism, gut microbiotaand/or inflammation inthe intestine, adipose tissue and cancer cells, which require intensive courseworkrelevant to the research. Meanwhile, the USDA fellows achieved the following award/honor/recognition. Awarded Lynn Fellowship Developed Functional Fiber product Hemp muffins for the American Society of Baking product develop competition ?Spring 2022 B.J Liska Outstanding Graduate Teaching Assistant Award
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Roy, K., Moncada Edward & Lavanya Reddivari. The onset and severity of colitis in IL-10 -/- mice is not dependent on fecal microbiota transplantation from ulcerative colitis patients. (2022). Submitted to Crohns Colitis Foundation annual meeting January 2023.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2022
Citation:
Eckrote, S., Nathan, V., Reddivari, L. (2022). Protective Effects of Blueberry Phenolics in IPEC-J2 Cells Challenged with Post Weaning Diarrhea Pathogens. Poster Summer College of Agriculture Research Foundation, Purdue University
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