Source: UNIVERSITY OF FLORIDA submitted to
REPRODUCTIVE BIOTECHNOLOGY CONCENTRATION AT UNIVERSITY OF FLORIDA
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
NEW
Funding Source
Reporting Frequency
Annual
Accession No.
1025156
Grant No.
2021-38420-34067
Project No.
FLA-ANS-006035
Proposal No.
2020-08154
Multistate No.
(N/A)
Program Code
KK
Project Start Date
Jan 15, 2021
Project End Date
Jan 14, 2026
Grant Year
2021
Project Director
Hansen, P. J.
Recipient Organization
UNIVERSITY OF FLORIDA
G022 MCCARTY HALL
GAINESVILLE,FL 32611
Performing Department
Animal Sciences
Non Technical Summary
Funds are available for six Fellowships and three IRTA toestablish a graduate program in reproductive biotechnology that educates a diverse group of MS students with the principles of animal reproduction and the technical competency required to excel in careers in animal production. Measurable objectives include a new concentration in reproductive biotechnology within existing graduate programs in Animal Sciences and Animal Molecular and Cell Biology, graduation of six MS Fellows, two from groups traditionally under-represented in agriculture, and an internship program. Features include the participation of 10 faculty, new courses providing experiential learning in reproductive biotechnology and a focus on interdisciplinary education involving reproduction, animal science, cell biology, genetics and biotechnology. Fellows will learn the experimental method through the development of a research program on a topic relevant to contemporary issues in reproductive biotechnology and applied reproduction. Fellows will gain experience of the practical application of reproductive biology by participation in an internship with a leading company involved in reproductive biotechnology or with a research team at Universidade de São Paulo that implements reproductive technology research programs on commercial farms. The measurable target, directly relevant to NNF program goals and USDA strategic goals, is the education of 6 MS-level scientists with the broad understanding, experience and enthusiasm needed to provide human capital for the continued prosperity of American agriculture. The development of a formal program in reproductive biotechnology will ensure that there will be a sustained pipeline for development of graduates in an important agricultural TESA.
Animal Health Component
10%
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3013899104010%
3013899103010%
3013899105020%
3013899101010%
3013899102050%
Goals / Objectives
Reproduction is key to optimizing the efficiency of animal-sourced food production. Indeed, much of the feed energy required to produce a unit of edible product goes to the female that produces the offspring for meat rather than to the offspring itself. In beef cattle, over 50% of the energy to produce a kilogram of beef is consumed by the dam of the feeder animal. Cow fertility is also an important driver of profitability of dairy production systems. Each day that a cow eligible for breeding is not pregnant costs $2.25 . Reducing the period a cow is not pregnant by 15 days would result in annual saving of $317 million for the US dairy industry. Reproductive technologies have become an important adjunct to new genetic strategies that increase accuracy of genetic selection and reduce generation interval . Indeed, new reproductive techniques like embryo transfer, sex sorting of semen, in vitro production of embryos, somatic cell nuclear transfer, gene editing, stem cell technologies and in vitro breeding are rapidly transforming the landscape of livestock genetics .The increasing impact of applied animal reproduction is expanding the demand for animal reproduction scientists in academia and industry.The entwining of traditional applied reproductive physiology with emerging technologies of embryology, stem cell biology, and genetic engineering means that education of tomorrow's reproduction specialist must be grounded in the principles of animal science and reproductive physiology but also with understanding of cell biology, genetics, and biotechnology. To meet the growing need for scientists with this background, it is the goal to form a new graduate concentration at the University of Florida called the Reproductive Biotechnology Concentration (RBC) designed to produce a diverse group of MS graduates educated in principles of animal reproduction and with the technical competency required to obtain and excel in academic and industry positions in animal agriculture. Measurable goals include the formation of the new concentration in reproductive biotechnology within existing graduate programs in Animal Sciences and Animal Molecular and Cell Biology, graduation of six MS Fellows, including at least two students two from groups traditionally under-represented in agriculture, an internship program that allows students to get non-academic experience in a related industry, and formation of two new courses on animal reproduction.
Project Methods
The typical student recruited into the program will have a strong background in animal science and the biological sciences and has the track record to indicate 1) an interest in pursuing a career in academia or industry related to animal reproduction and 2) the intellectual tools and capacity to succeed in today's competitive scientific environment.A core value of the faculty is collaboration between laboratories, both within- and across disciplines. This feature of the program, in concert with the diversity of faculty and students in terms of research focus, geographical origin, and previous educational experiences, creates an environment where innovation and collaboration are central to the research activities of the program. Faculty in the seek to develop students who are integrationists that can combine concepts of physiology, genetics, cell biology, molecular biology, biochemistry and animal management to develop cutting-edge approaches for improving animal production and health. This approach to science is cultivated through many avenues including formal course work, journal clubs, seminars and research symposia, informal didactic teaching, extensive collaborations across disciplines, performance of supervised research towards the student's dissertation, and opportunities for internships in other academic laboratories and biotechnology companies.The major professor, who serves as Chair of the student's MS Supervisory Committee, has responsibility for directing the student's research and, along with the Supervisory Committee (consisting of three faculty members in total), provides advice regarding a required plan of formal course work.The MS degree requires 30 credits, of which 24 credits must be coursework. Typical courses include statistics, biochemistry, physiology, reproductive physiology, nutrition, cell biology, and immunology. Two new courses will be created to increase competence of Fellows for practicing a wide variety of techniques central to reproductive biotechnology. The new courses will be Applied Reproductive Biotechnology (3 credits) and Embryo Transfer Practicum (2 credits).Students mix the practice of science at the bench or in the field with a variety of intellectual exercises in journal clubs and seminars that not only expose students to cutting-edge concepts in modern biology but which are also an avenue for developing critical thinking skills important for the ability to develop hypotheses, plan research, and interpret data. Seminars are also used to allow students to gain skills at oral communication and learn the value of scientific scrutiny of ideas. Students are expected to participate in three weekly seminar series - the Animal Sciences departmental seminar, the Animal Molecular and Cellular Biologyseminar, and the Reproductive & Perinatal Biology Seminar hosted by the D.H. Barron Reproductive and Perinatal Biology Research Program.A unique and fundamental aspect of the programwill be providing graduate students with the opportunity to do an internship with an organization heavily involved with one or more reproductive technologies. The goal is to give students experience with the practice of applied reproduction in a commercial setting so that they can better understand the role of reproductive biotechnology in animal agriculture and make informed decisions about future careers.Another unique feature will be the availability of an international study research allowance for support of research experiences in Brazil. This country is a world leader in the application of reproductive technologies to large-scale beef and dairy cattle operations. Often, fertility trials require large numbers of animals per group to have suitable statistical power. Brazil is one of the few places in the world where these studies can be performed. Selected Fellows will spend 2-3 months involved in hands-on activities associated with reproductive management of large beef operations in Brazil. Each Fellow will be paired with a bilingual graduate student from the University of Sao Paulo and participate in applied research conducted by faculty at that university.Research activities begin early in a student's program. The goal is to learn how to conduct research through interactive experience with the major professor, the Supervisory Committee, and fellow students in research topics of reproductive biotechnology. Among the major objectives are 1) to expose students to a wide range of concepts and techniques through interaction with other laboratories and 2) gain experience working with animals of agricultural importance. The Dept. of Animal Sciences maintains extensive farm facilities for beef and dairy cattle, horses, sheep and pigs. Most students conduct a portion of their research on a cooperating farm or ranch. Faculty are also heavily involved in research designed to generate commercial products of use to the livestock industry and veterinary community. Among major research partners are Balchem, Zoetis, Elanco, Church & Dwight, DSM Nutrition, Phibro, and Select Sires. Research conducted by thefaculty encompasses a large diversity of research topics and experimental approaches.Among the techniques utilized in their laboratories are ultrasound guided oocyte pick-up, in vitro production of embryos, oocyte freezing, embryo transfer, embryo flushing, uterine sampling, cell culture, stem cell derivation, gene editing, analysis of the transcriptome and methylome, hormone immunoassays, pharmacological control of the estrous cycle and timed artificial insemination.Many of the faculty in the program have significant extension appointmentsand all faculty participate in extension activities. Students are encouraged to obtain extension experience by participating in extension meetings or by preparing publications for producer groups.

Progress 01/15/23 to 01/14/24

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Each student is heavily involved in course work, attendance at seminars, and research. Several students have presented their research at scientific meetings and some have submitted papers for publication. One student has taken advantage of the internship program to do a research internship at Colorado Center for Reproductive Medicine. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?continue to educate the remaining students until they complete the degree

Impacts
What was accomplished under these goals? Year 3of the project has involved two major activities - participation of fellows in research and educational activities (classes, attending seminars and meetings) and graduation of the first students from the program. Threeof the graduate students have recently graduated - all three are pursuing a PhD. Also, note that 2 of the 6 students are African-Americanand all six students are female. Details of each student follow. Meghan Campbell (Angela Gonella Diaza, supervisor) Graduate Program: Animal Sciences Defense of MS Thesis: 6/29/2023 (successful) Research: Meghan applied targeted metabolomics to discover markers in the plasma of prepubertal Brahman heifers and bulls that predicted sexual maturity and reproductive success as yearlings. Update: Starting a PhD at UF Department of Family, Youth and Community Sciences Jillian Guertin, MS (Brad Daigneault, supervisor) Graduate Program: Animal Sciences Expected Graduation: Fall 2024 Research: Jillian is working to optimize conditions for stallion sperm compatible for equine reproductive technologies including in vitro fertilization, prolonged maintenance of cooled stallion sperm for artificial insemination, and alternative immunocontraceptive approaches. Quinn Hoorn (Peter Hansen, supervisor) Graduate Program: Animal Molecular and Cellular Biology. Graduated inMay 2024. Research: Deciphering genetic and physiological determinants of maternal physiology that affect pregnancy outcomes in cattle. Update: Threepapers submitted. seeking a doctoral position. Kamryn Joyce (Mario Binelli, supervisor) Graduate Program: Animal Sciences Defense of MS Thesis: 06/29/2023 (successful). Graduation ceremony: 08/12/2023 Research: Transcriptomic response to seasonal variations on oocytes and Granulosa cells in beef cows. Update: Kamryn movedto Virginia tech to pursue her PhD. She was awarded The George Washington Carver Assistantship. Madison L. Newman (Rafael Bisinotto, supervisor) Graduate Program: Animal Molecular and Cellular Biology Expected Graduation: July 2024 Research: Use of a digital vaginoscope to guide cervical swab and cytology for an IFN-tau based pregnancy diagnosis test for cattle. Olivia Ohm (J Block supervisor) Graduate Program: Animal Science, Univ Wyoming Expected Graduation: May 2025 Research: Determine the effects of feeding alginate oligosaccharide on the composition of the bull reproductive microbiome, semen composition and sperm viability.

Publications


    Progress 01/15/22 to 01/14/23

    Outputs
    Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Each student is heavily involved in course work, attendance at seminars, and research. Several students have presented their research at scientific meetings and some have submitted papers for publication. One student has taken advantage of the internship program to do a research internship at Colorado Center for Reproductive Medicine. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?By next year, most students will have graduated or will be close to graduation.

    Impacts
    What was accomplished under these goals? Year 2of the project has involved two major activities - completion of recruitment of Fellows, participation of fellows in research and educational activities(classes, attending seminars and meetings) and enrollment of students in the new interdisciplinary reproductive biotechnology graduate concentration for MS at the University of Florida. All 6 Fellows have now been recruited. Note that 4 of the 6 students are in Animal Science programs and 2 are in Animal Molecular and Cellular Biology. Two of the graduate students have recently graduated - both are pursuing a PhD. Also, note that 2 of the 6 students are African-American (the two pursuing a PhD) and all six students are female. Details of each student follow. Meghan Campbell (Angela Gonella Diaza, supervisor) Graduate Program: Animal Sciences Defense of MS Thesis: 6/29/2023 (successful) Research: Meghan applied targeted metabolomics to discover markers in the plasma of prepubertal Brahman heifers and bulls that predicted sexual maturity and reproductive success as yearlings. Update: Starting a PhD at UF Department of Family, Youth and Community Sciences Jillian Guertin, MS (Brad Daigneault, supervisor) Graduate Program: Animal Sciences Expected Graduation: Fall 2024 Research: Jillian is working to optimize conditions for stallion sperm compatible for equine reproductive technologies including in vitro fertilization, prolonged maintenance of cooled stallion sperm for artificial insemination, and alternative immunocontraceptive approaches. Quinn Hoorn (Peter Hansen, supervisor) Graduate Program: Animal Molecular and Cellular Biology Expected Graduation: May 2024 Research: Deciphering genetic and physiological determinants of maternal physiology that affect pregnancy outcomes in cattle. Update: Two papers submitted, one in preparation Kamryn Joyce (Mario Binelli, supervisor) Graduate Program: Animal Sciences Defense of MS Thesis: 06/29/2023 (successful). Graduation ceremony: 08/12/2023 Research: Transcriptomic response to seasonal variations on oocytes and Granulosa cells in beef cows. Update: Kamryn is moving to Virginia tech to pursue her PhD. She was awarded The George Washington Carver Assistantship. Madison L. Newman (Rafael Bisinotto, supervisor) Graduate Program: Animal Molecular and Cellular Biology Expected Graduation: July 2024 Research: Use of a digital vaginoscope to guide cervical swab and cytology for an IFN-tau based pregnancy diagnosis test for cattle. Olivia Ohm (J Block supervisor) Graduate Program: Animal Science, Univ Wyoming Expected Graduation: May 2025 Research: Determine the effects of feeding alginate oligosaccharide on the composition of the bull reproductive microbiome, semen composition and sperm viability.

    Publications


      Progress 01/15/21 to 01/14/22

      Outputs
      Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Students are beginning their programs - taking course work and starting research activities. More will be available to report next year. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?By next year, it is anticipated that a total of 6 fellowships will be awarded. Some of the students will be nearly half way through their programs.

      Impacts
      What was accomplished under these goals? Year 1 of the project has involved two major activities - recruitment of Fellows and formation of a formal interdisciplinary reproductive biotechnology graduate concentration for MS and PhD students at the University of Florida. For the first activity, a total of five students have been admitted to the program - four from the University of Florida and one from University of Wyoming. Two of these students began study in fall 2021and the other three are starting graduate school in spring 2022. For the second activity, the University of Florida approved the reproductive biotechnology concentrations for both MS and PhD students. Final approval by the Graduate Council was in November 2021.

      Publications