Recipient Organization
IOWA STATE UNIVERSITY
2229 Lincoln Way
AMES,IA 50011
Performing Department
Veterinary Diagnostic and Production Animal Medicine
Non Technical Summary
Viral arthritis and tenosynovitis in poultry caused by Avian Reovirus (ARV) has caused significant losses in both broiler chickens and market turkeys. The ubiquitous nature of ARV makes usual diagnostic methods of virus isolation and RNA detection methods insufficient to prove its involvement in this disease. In this study, we aim to develop and validate diagnostic assays that do not merely stop at detection of ARV, but rather can show the involvement of ARV in the microscopic lesions. Immunohistochemistry and in-situ hybridization are techniques that are able to demonstrate ARV antigens or nucleic acid in the arthritis and tenosynovitis in poultry. If successful, these new methodologies will be effective, timely and definitive diagnostic assays for ARV. The developed diagnostic assays' sensitivity, specificity and limit of detection will be compared to currently used diagnostic assays, like virus isolation and real time PCR. The output of this project will drastically improve the ability of poultry professionals, poultry clinicians and diagnosticians, poultry researchers and poultry diagnostic laboratories to accurately diagnose ARV cases and better elucidate the poorly understood parts of its pathogenicity and epidemiology.
Animal Health Component
10%
Research Effort Categories
Basic
10%
Applied
10%
Developmental
80%
Goals / Objectives
The major goal of this project is to develop and validate Immunohistochemistry (IHC) and in-situ hybridization (ISH) as simple and effective diagnostic tools for Avian Reovirus (ARV) as an etiological agent for viral arthritis tendonitis and tenosynovitis in poultry.Objectives:Generate a panel of antigen peptides representing unique domains of the p10, p17 and σC proteins (Sigma 1 gen) that allows differential diagnosis of ARV of different origins.Avian Orthoreovirus polyclonal and monoclonal S1 monoclonal antibodies production.Immunohistochemistry optimization.Development of a differential nucleotide in situ hybridization.
Project Methods
Immunohistochemistry and in-situ hybridization are techniques that are able to demonstrate Avian Reovirus (ARV) antigens or nucleic acid in the arthritis and tenosynovitis in poultry. The developed diagnostic assays' sensitivity, specificity and limit of detection will be compared to currently used diagnostic assays, like virus isolation and real time PCR. The output of this project will drastically improve the ability of poultry professionals, poultry clinicians and diagnosticians, poultry researchers and poultry diagnostic laboratories to accurately diagnose ARV cases and better elucidate the poorly understood parts of its pathogenicity and epidemiology. The results and science-based knowledge will be delivered through scientific conferences, publications and outreach efforts.