Source: UNIVERSITY OF DELAWARE submitted to NRP
ACQUISITION OF A SEAHORSE XFE96 ANALYZER TO CATALYZE RESEARCH AND EDUCATION
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
OTHER GRANTS
Reporting Frequency
Annual
Accession No.
1024363
Grant No.
2020-70410-32903
Cumulative Award Amt.
$225,537.00
Proposal No.
2020-07761
Multistate No.
(N/A)
Project Start Date
Sep 1, 2020
Project End Date
Aug 31, 2022
Grant Year
2020
Program Code
[EGP]- Equipment Grants Program
Recipient Organization
UNIVERSITY OF DELAWARE
(N/A)
NEWARK,DE 19717
Performing Department
Animal and Food Sciences
Non Technical Summary
This proposal describes our intent to acquire the Agilent Seahorse XFe96 Analyzer. The instrument measures mitochondrial respiration (mitochondria are the powerhouse of the cell and analysis of its respiration rates allows investigation of how the cell is behaving metabolically) and glycolysis (this is a critical energy pathway in the body and analysis of its activity leads to information on how efficiently the cells are utilizing energy resources) in a 96-well microplate format (allows for analysis of a large number of samples at a time which enables high-throughput experimental pipelines) to deliver functional information about the metabolic state in live cells (a key advantage of this technology is that all analysis is done in live cells, and is thus physiologically relevant). The Seahorse XFe96 Analyzer's strength lies in the broad range of projects to which it can be successfully applied. The research projects this instrument will enable include those that address Sustainable Ag Intensification (plant and animal production, health, and genetics), Ag Climate Adaptation (climate research and resiliency), Food and Nutrition Translation (food safety and health, nutrition and health promotion), Value-Added Innovations (infrastructure, innovation, and well-being), and Ag Science Policy Leadership (beacon for science). We also propose the use of this instrument for other federally-funded projects from the NIH, NSF, DOD, and commercial agri-businesses/life science companies in the region. Lastly, we have modules in the grant proposal that will enable access and training on the instrument for historically underrepresented minorities. We (the University of Delaware) has specifically partnered with Lincoln University (the nation's first historically black degree granting university) to enable these outreach efforts in addition to our USDA/NIFA funded UD Envision program that allows historically underrepresented minorities hand-on-experiences in research at University of Delaware research laboratories.
Animal Health Component
20%
Research Effort Categories
Basic
70%
Applied
20%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1024099106010%
1320430101010%
3113299102030%
7124099115015%
7223999109015%
7214099104020%
Knowledge Area
102 - Soil, Plant, Water, Nutrient Relationships; 311 - Animal Diseases; 132 - Weather and Climate; 712 - Protect Food from Contamination by Pathogenic Microorganisms, Parasites, and Naturally Occurring Toxins; 722 - Zoonotic Diseases and Parasites Affecting Humans; 721 - Insects and Other Pests Affecting Humans;

Subject Of Investigation
3999 - Animal research, general; 4099 - Microorganisms, general/other; 3299 - Poultry, general/other; 0430 - Climate;

Field Of Science
1040 - Molecular biology; 1090 - Immunology; 1060 - Biology (whole systems); 1010 - Nutrition and metabolism; 1020 - Physiology; 1150 - Toxicology;
Goals / Objectives
This proposal describes our intent to acquire the Agilent Seahorse XFe96 Analyzer. The instrument measures mitochondrial respiration and glycolysis in a 96-well microplate format to deliver functional information about the metabolic state in live cells. The Seahorse XFe96 Analyzer's strength lies in the broad range of projects to which it can be successfully applied. It will be an integral part of the Delaware Biotechnology Institute's Bioimaging Center - Flow Cytometry Core. The instrument will be available to all researchers both at the University of Delaware and regionally (academic institutions and industry) and is intended to be an open user facility that charges minimal user fees to sustain extended support of the instrument. The research projects this instrument will enable include those that address Sustainable Ag Intensification (plant and animal production, health, and genetics), Ag Climate Adaptation (climate research and resiliency), Food and Nutrition Translation (food safety and health, nutrition and health promotion), Value-Added Innovations (infrastructure, innovation, and well-being), and Ag Science Policy Leadership (beacon for science).
Project Methods
Given the importance of the Seahorse XFe96 Analyzer in enabling cutting-edge metabolomic research and teaching at the University of Delaware and the region, the University of Delaware is fully committed to the proposed instrument. The University considers the Seahorse XFe96 Analyzer as a critical equipment to foster inter-disciplinary research and thus has provided space at the newly constructed 200,000 sq. ft. Ammon-Pinizzotto Biopharmaceutical Innovation Center, which it envisions as a hub for "One Health". The instrument will be supervised by the Delaware Biotechnology Institute's Bioimaging Center - Flow Cytometry Core, which has the expertise and resources to support such a vital instrument throughout its lifecycle. Moreover, the university has committed both employee effort and financial support to enable successful operation of the proposed Seahorse XFe96 Analyzer.The proposed grant application is budgeted for two years. Once awarded, the process of equipment ordering and delivery will be initiated with Agilent Technologies, the makers of the Seahorse XFe96 Analyzer. We expect the process to be completed within a month (estimated delivery time is less than a week). This process includes ordering the equipment, delivery of equipment, professional set-up and calibration, and extensive hands-on training from Agilent service engineers. This equipment will also be connected to the Delaware Biotechnology Institute's iLab system (Agilent Technologies) that allows seamless reservation, usage tracking, and billing. The Seahorse XFe96 Analyzer comes with a year of warranty and on-site support. The University of Delaware has committed to a second year of extended support (see letter of support, and Agilent quote for extended warranty) and staff support across the lifetime of the equipment (see letter of support). Beyond two years, the instrument should become self-sustaining. Internal rates will be applied to users at UD and Lincoln University (our collaborators on the grant), external rates to other academic and non-profit organizations, and commercial rates to commercial companies. The higher rates for commercial users, were established to give priority to academic users. Usage time is expected to be less during the first year because projects need to be started and users need to be trained. The Seahorse XFe96 Analyzer comes with a one-year warranty. UD will further support coverage for an additional year as mentioned in the institutional support letter. The core will then get a renewable annual service contract (~$22,000 /year, see equipment quote). The hourly rates and financial models are calculated to reach >98% recovery. If there is a surplus, it will be used to lower rates in the subsequent year. Any remaining deficit will be small and will be covered by institutional commitments.

Progress 09/01/20 to 08/31/22

Outputs
Target Audience:December 2020: Quote for Seahorse XFe96 Analyzer obtained from Agilent Technologies - sole suppliers of the instrument December 2020: Purchase order generated to initiate purchase of instrument January 2021: Instrument delivered to University of Delaware campus in Newark, DE January 2021: Instrument installed at the Flow Cytometry Core of Delaware Biotechnology Institute, Ammon-Pinizzotto Biopharmaceutical Innovation Center on the University of Delaware STAR Campus. February 2021: Hands-on training for faculty from University of Delaware and Lincoln University February 2021 - 2023: Multiple principal investigators, research scientists, and graduate students have been trained and use the instrument on a regular basis on a pay-per-use model. These include reserachers from UD Animal and Food Sciences (Aditya Dutta, Kathryn Ellwood, Ashley Taylor, Mark Parcells, Amy Biddle, Ryan Arsenault), UD Kinesiology and Applied Physiology (David Edwards,Christopher Martens,Ibra Fancher,Thanh Nguygen,Theodore DeConne,Justin Mehrer), UD Medical and Molecular Sciences (Vijay Parashar),UD Biology (Ramona Neunuebel,Marina Grossi,Jessica Tanis,Melinda Duncan), UD Plant and Soil Sciences (Nicole Donofrio), UD Chemical and Biomolecular Engineering (Catherine Fromen), UD Biomedical Engineering (Jason Gleghorn), UD Chemical Engineering (TerryPapoutsakis,Aditya Kunjapur), and Lincoln University Biology (Karen Baskerville) Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Other than training and providing access to the instrument, this grant has allowed the establishmnet of comprehensive research teams. An example of this is the team formed to understand the genetic underpinnings of stuttering. This involves expertisein brain imaging (Ho Ming Chow - UD Communication Sciences and Disorders), expertise in social behaviors (Joshua Neunuebel - UD Psychological and Brain Sciences), and expertise in mitochondrial metabolomics (Aditya Dutta - UD Animal and Food Sciences). This team would not be possible if they did not have access to the instrumnet to study mitochondrial energy changes invoved in stuttering. How have the results been disseminated to communities of interest?Data generated using the machine has already led to 2 peer-reviewed publications, 4 talks, and 3 conference abstracts (details indicated under "products"). What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? December 2020: Quote for Seahorse XFe96 Analyzer obtained from Agilent Technologies - sole suppliers of the instrument December 2020: Purchase order generated to initiate purchase of instrument January 2021: Instrument delivered to University of Delaware campus in Newark, DE January 2021: Instrument installed at the Flow Cytometry Core of Delaware Biotechnology Institute, Ammon-Pinizzotto Biopharmaceutical Innovation Center on the University of Delaware STAR Campus. February 2021: Hands-on training for faculty from University of Delaware and Lincoln University February 2021 - 2023: Multiple principal investigators, research scientists, and graduate students have been trained and use the instrument on a regular basis on a pay-per-use model.These include reserachers from UD Animal and Food Sciences (Aditya Dutta, Kathryn Ellwood, Ashley Taylor, Mark Parcells, Amy Biddle, Ryan Arsenault), UD Kinesiology and Applied Physiology (David Edwards,Christopher Martens,Ibra Fancher,Thanh Nguygen,Theodore DeConne,Justin Mehrer), UD Medical and Molecular Sciences (Vijay Parashar),UD Biology (Ramona Neunuebel,Marina Grossi,Jessica Tanis,Melinda Duncan), UD Plant and Soil Sciences (Nicole Donofrio), UD Chemical and Biomolecular Engineering (Catherine Fromen), UD Biomedical Engineering (Jason Gleghorn), UD Chemical Engineering (TerryPapoutsakis,Aditya Kunjapur), and Lincoln University Biology (Karen Baskerville)

Publications

  • Type: Journal Articles Status: Accepted Year Published: 2021 Citation: Lam AB, Kervin K, and Tanis JE. 2021. Vitamin B12 Impacts Amyloid-? Induced Proteotoxicity by Regulating the Methionine/S-adenosylmethionine Cycle. Cell Reports, 36:109753. PMID 34592146
  • Type: Journal Articles Status: Accepted Year Published: 2021 Citation: Papachristodoulou A, Rodriguez-Calero A, Panja S, Margolskee E, Virk RK, Milner TA, Martina LP, Kim JY, Di Bernardo M, Williams AB, Maliza EA, Caputo JM, Haas C, Wang V, De Castro GJ, Wenske S, Hibshoosh H, McKiernan JM, Shen MM, Rubin MA, Mitrofanova A, Dutta A, Abate-Shen C. NKX3.1 Localization to Mitochondria Suppresses Prostate Cancer Initiation. Cancer Discov. 2021 Sep;11(9):2316-2333. doi: 10.1158/2159-8290.CD-20-1765. Epub 2021 Apr 23. PMID: 33893149; PMCID: PMC7611624.
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2022 Citation: Christopher Martens (Assistant Professor). Getting to the Heart of Alzheimers Disease: Vascular Determinants of Age-Related Memory Loss Department of Health and Exercise Science, Colorado State University, Fort Collins, CO (Invited Talk).
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2022 Citation: Theodore DeConne (Graduate Student). The Effect of LDL-C on T-cell Function and its Implications for Cerebrovascular Health. Department of Kinesiology Seminar, University of Illinois Chicago, Chicago, IL, USA. (Invited Talk).
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2022 Citation: Theodore DeConne (Graduate Student).The Effect of Low-Density Lipoprotein Cholesterol on T-Cell Mitochondrial Respiration. 2022 Mid-Atlantic American College of Sports Medicine (MARC ACSM) Annual Meeting, Harrisburg, PA, USA (Oral Presentation)
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2022 Citation: Theodore DeConne (Graduate Student). The effect of LDL-C on T-cell metabolism and its implication for vascular function. Department of Kinesiology Seminar, University of Texas at Arlington, Arlington, TX, US (Oral Presentation).
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2023 Citation: DeConne, T. M., Ward, H. R., Edwards, D. G., Fancher, I. S., Trott, D. W., & Martens, C. R. (2023). The effect of low-density lipoprotein cholesterol on t-cell mitochondrial respiration. APS Summit. Long Beach, CA
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2022 Citation: Ellwood K, Taylor A, Dutta A. Glycerolipid metabolism is a key early-detection biomarker for spontaneous ovarian cancer onset. AACR Special Conference on Precision Prevention, Early Detection, and Interception of Cancer. November 17-19, 2022, Austin, TX
  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2023 Citation: Taylor A, Ellwood K, Li CJ, Dutta A. Transcriptomic Data Reveals MYC as an Upstream Transcriptional Regulator in Laying Hen Pre-recruitment Follicles using Ingenuity Pathway Analysis. Plant and Animal Genome Conference. Jan 12-18, 2023, San Diego, CA


Progress 09/01/20 to 08/31/21

Outputs
Target Audience:December 2020: Quote for Seahorse XFe96 Analyzer obtained from Agilent Technologies - sole suppliers of the instrument December 2020: Purchase order generated to initiate purchase of instrument January 2021: Instrument delivered to University of Delaware campus in Newark, DE January 2021: Instrument installed at the Flow Cytometry Core of Delaware Biotechnology Institute, Ammon-Pinizzotto Biopharmaceutical Innovation Center on the University of Delaware STAR Campus. February 2021: Hands-on training for faculty from University of Delaware and Lincoln University February 2021 to present: Multiple principal investigators, research scientists, and graduate students have been trained and use the instrument on a regular basis on a pay-per-use model Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?February 2021: Hands-on training for faculty from University of Delaware and Lincoln University February 2021 to present: Multiple principal investigators, research scientists, and graduate students have been trained and use the instrument on a regular basis on a pay-per-use model How have the results been disseminated to communities of interest?February 2021: Hands-on training for faculty from University of Delaware and Lincoln University February 2021 to present: Multiple principal investigators, research scientists, and graduate students have been trained and use the instrument on a regular basis on a pay-per-use model What do you plan to do during the next reporting period to accomplish the goals?Moreprincipal investigators across academia and industry, research scientists, and graduate students will be trained and made familiar with the availability and utilization of the instrumnet.

Impacts
What was accomplished under these goals? December 2020: Quote for Seahorse XFe96 Analyzer obtained from Agilent Technologies - sole suppliers of the instrument December 2020: Purchase order generated to initiate purchase of instrument January 2021: Instrument delivered to University of Delaware campus in Newark, DE January 2021: Instrument installed at the Flow Cytometry Core of Delaware Biotechnology Institute, Ammon-Pinizzotto Biopharmaceutical Innovation Center on the University of Delaware STAR Campus. February 2021: Hands-on training for faculty from University of Delaware and Lincoln University February 2021 to present: Multiple principal investigators, research scientists, and graduate students have been trained and use the instrument on a regular basis on a pay-per-use model

Publications