Center of Excellence for Post-Harvest Technologies
Non Technical Summary
Conventional disinfection methods used to inactivate viruses, such as, ethanol, hypochlorite, and quaternary ammonium formulations have shown to be ineffective on select food contact and fomite surfaces. Therefore, alternative antiviral agents such as, phytochemicals have received attention as potential norovirus inhibitors due to their relatively low toxicity and lack of side effects, which allows them to be prepared as food-safe formulations. The goal of the proposed project is to determine whether hemp and mushroom plant extracts can be used as an alternative antiviral and disinfectant replacement for the control of novel SARS-Corona Virus-2 (SARS-CoV-2) on food contact and fomite surfaces.
Animal Health Component
Research Effort Categories
Goals / Objectives
Establish the most efficient extraction method from the hemp (HPE) or mushroom (MPE) plant crude extract with maximum phenolic and flavonoid contents and antioxidant activities using different solvents.Determine the antiviral activities of hemp and mushroom extract against novel SARS-CoV-2 in an in vitro cell culture assay.Measure the transfer rates of SARS-CoV-2 to different contact surfaces treated with hemp or mushroom plant extracts compared to conventional commercial (hydrogen peroxide and sodium hypochlorite).
The project objectives will be accomplished by team members with experience in mycology, microbiology, nutritional biochemistry, functional foods and bioactive components, immunology, food safety, horticulture and plant science. The task includes determining the most effective method(s) for extraction and isolation of hemp and mushroom plant polyphenolic and bioactive compounds. This will include untargeted profiling of key polyphenolic classes will be targeted: anthocyanins (quantified as cyanidin equivalents), flavones (quantified as luteolin equivalents), flavonols and flavanols (quantified as catechin equivalents), lignans (quantified as sesamin equivalents), alkylphenols (quantified as 5-pentadecylresorcinol equivalents), stilbenes (quantified as resveratrol equivalents), low-molecular-weight phenolics (quantified as tyrosol equivalents), and phenolic acids (quantified as ferulic acid equivalents).To determine the antiviral properties of hemp and mushroom extracts, a mammalian tissue culture-based bioassay will be utilized to determine the half maximum inhibitory (IC50) concentrations, cytotoxicity and plaque inhibition of SARS-CoV-2 tested against different concentrations of plant extracts. This will also include determining the transfer and transmission rate(s) of SARS-CoV-2 on different types of contact (stainless steel, cardboard, pvc, copper) or fomite surfaces compared to conventionally used disinfectants.