Source: COLLEGE OF MICRONESIA submitted to NRP
CROWN LEAF BUDDING, ROOTING AND GROWING MEDIA FOR PINEAPPLE (ANANAS COMOSUS (L) MERR.
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1023611
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Aug 1, 2020
Project End Date
Sep 30, 2022
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
COLLEGE OF MICRONESIA
PO BOX 1179
KOLONIA POHNPEI,FM 96941
Performing Department
COLLEGE ADMINISTRATION
Non Technical Summary
SummaryPineapple (Ananas comosus (L) Merr .) is an under-utilized crop at the Federated States of Micronesia (FSM) despite its multifarious uses as food, animal feeds, industrial and medicinal sources of raw materials, it has an import- substitution potential.One primary reason for its under-utilized status is lack of planting materials at farm level. Subsistence farmers as the common sources of pineapple in the local markets sell few pineapple fruits with intact crowns. This crown contains about 50-70 leaves that could be sources of next planting materials. Hence, this project will study the potential of using the crown leaves of existing pineapple cultivars in Chuuk. This project will have two-fold objectives. Objective 1 will compare the rooting and growth performances of native and introduced pineapple cultivars using different rooting media. Their crown leaves will be grown in five rooting media: garden soil (Control), coarse sand; coconut coir, garden soil and coarse sand (1:1, v/v) and garden soil and coconut coir (1:1, v/v). Objective 2 will determine the vegetative and reproductive development of the two cultivars grown in different growing media under pot containers. The potting media to be used are garden soil (Control); garden soil and coconut coir (1:1, v/v); garden soil and coarse sand (1:1, v/v) and garden soil, coconut coir and coarse sand (1:1:1, v/v/v). All experiments will be conducted in the screen house and laid out in Randomized Complete Block Design (RCBD) in factorial arrangements with four replications: 2 cultivars and five rooting media in Objective one and 2 cultivars and 4 growing media in Objective two.ANOVA for RCBD in factorial arrangements and Fisher's Least Significant Differences (LSD) test will be used for determining significant differences among the treatments.
Animal Health Component
50%
Research Effort Categories
Basic
30%
Applied
50%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2021020102010%
2021020106020%
2051020102020%
2051020106030%
2061020102010%
2061020106010%
Goals / Objectives
Goal and ObjectivesThe goal of this project is increased local pineapple production for enhancement of pineapple industry at the Federated States of MicronesiaObjectivesTo compare capacity of local and introduced pineapple cultivars in producing plantlets from crown leaf budding using different rooting mediaTo determine growth and development of the crown leaf- budded plantlets grown in different growing media
Project Methods
Study AreasGeneral ActivitiesDetermination of plantlet production of two cultivars with crown leaves grown in five rooting mediaData to be gathered from 5 sample plants/ treatment/replication: survival rate (%), number of plantlets/treatment, number of leaves/plant per treatment, leaf area of sample plants, number of roots of sample plants/treatment, root length of sample plants.Determination of growth and development of two cultivar plantlets produced from different rooting media and to be tested for performances in four growing media Data to be gathered from 5 sample plants/treatment/replication: length and width of longest leaves, number of developed suckers and ratoons, days/months to flowering and fruiting, fruit weight, fruit size, crown weight, plant height every month, number of leaves made every month, number of months before first sucker and slip are madeStatistical AnalysesAnalyses of Variance for Randomized complete Design in factorial arrangements with four replications: 2 cultivars and five rooting media in Objective one and 2 cultivars and 4 growing media in Objective two. Fisher's Least significant differences at p=0.05 will be used in treatment comparisons.Treatments and Experiment LayoutThis project will be composed of two components: 1.Comparison of plantlet production by crown leaf budding in two pineapple cultivars using 5 rooting media and 2. Evaluation of growth and development in two cultivar plantlets from different rooting substrates and then grown in various growing media.Objective 1. To compare capacity of local and introduced pineapple cultivars in producing plantlets from crown leaf budding using different rooting mediaThe treatments will be: Cultivars designated as C=== C1 (local variety) C2 (Hawaiian variety) Rooting media designated as R=== R1 (garden soil, control) R2 (coarse sand) R3 (coconut coir) R4 (garden soil + coarse sand 1:1, v/v) R5 (garden soil+ coconut coir 1:1, v/v) Combination treatments: C1R1 C1R2 C1R3 C1R4 C1R5 C2R1 C2R2 C2R3 C2R4 C2R5These treatments will be laid out in Randomized Complete Block (factorial arrangements) with four replications in a screen houseData to be collected from five samples for every treatment in each replication:Percent plantlet survival. Count number of surviving plantlets divided by total crown leaves planted x 100.Number of plantlet leaves. Count total number of leaves in each plantlet in respective treatments and replications.Area of D-leaf. Measure the center leaf for width and base to tip of D-leaf (highest leaf and stands straight up) for each sample plantlet in respective treatments and replications.Number of roots. Count total number of roots in sample plantlets in respective treatments and replications.Length of roots. Measure four longest roots in each sample plantlet in respective treatments and replications.Objective 2. To determine growth and development of the crown leaf- budded plantlets grown in different growing mediaThe treatments will be:Cultivars designated as C=== C1 (local variety) C2 (Hawaiian variety)Growing media designated as G: == G1 (garden soil, control) G2 (garden soil + coconut coir (1:1, v/v) G3 (garden soil+ coarse sand (1:1, v/v) G4 (garden soil + coconut coir +coarse sand 1:1:1:1, v/v/v) Treatment combinations: C1G1 C1G2 C1G3 C1G4 C2G1 C2G2 C2G3 C2G4Data to be collected from five samples for every treatment in each replication:Area of D-leaf. Measure the center leaf for width and base to tip of D-leaf (highest leaf and stands straight up) for each sample plantlet in respective treatments and replications.Plant height every month. Measure from base of plant up to the tip of crown.Number of leaves made every month. Count new emerging leaves monthly.Number of months before first sucker and slip appearance. Record from months after planting plantlets.Number of developed suckers and ratoons. Count these plant structures before harvesting fruits.Months to flowering and fruiting. Count number of months for the first appearance of flowers and fruiting.Fruit weight. Weigh freshly harvested fruits without crown.Crown weight. Weigh freshly cut crown.Statistical analyses. Analyses of Variance for Randomized complete Design in factorial arrangements with four replications: 2 cultivars and five rooting media in Objective one and 2 cultivars and 4 growing media in Objective two. Fisher's Least significant differences at p=0.05 will be used in treatment comparisons.

Progress 08/01/20 to 09/30/20

Outputs
Target Audience:Farmers, gardeners, homemakers, hobbyists, village leaders, schools, teachers, students, extension agents Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Training The brochures on pineapple provided the target audience brief profile of crops including plant description, recommended cultural management, nutritive values, health benefits, uses and one nutritious recipe using local produce as ingredients. Interested individuals learned news practical ways of multiplying pineapple plantlets from a pineapple crown. b. Professional development Target audience develops awareness about the necessary cultural management practices and uses to cultivate their local pineapple. They will be able to produce more seedlings from pineapple crown, which traditionally will produce one pineapple plant and one fruit. How have the results been disseminated to communities of interest?Dissemination of results was accomplished through partnership with the Department of Agriculture, women's groups, community leaders and churches during training, meetings and other community events. What do you plan to do during the next reporting period to accomplish the goals?Once the two screen houses and coconut coir ordered outside Chuuk are delivered, we will perform the production of pineapple plantlets from five rooting media (garden soil, coarse , coconut coir, garden soil + coarse sand 1:1, v/v and garden soil+ coconut coir 1:1, v/v).

Impacts
What was accomplished under these goals? Major activities completed The project started with the purchase of supplies (rooting and growing media, fertilizers, insecticides, polyethylene bags and gardening gadgets) and equipment and acquisition of pineapple crown of one popularly grown cultivar from the local markets , which sold the pineapple fruits in August to November in Chuuk. We tried leaf extraction from pineapple crown using leaves with and without stem attachments. These leaf extracts were grown in sterilized soil and coarse sand.. Seedlings from preliminary leaf extraction were planted in potting mix for observation of monthly growth. Specific objectives met - b.1. Objective No. 1: Our preliminary trial on leaf extraction with and without attached stems to the crown leaves produced no plantlets in crown leaves without stem attachment to the crown leaves. Meanwhile those crown leaves with stems attached produced plantlets: 42 % from garden soil and 25% from sand. b.2. Objective No. 2: To determine growth and development of the crown leaf- budded plantlets grownin different growing media One month after sprouting, plantlet roots from sand produced an average of 2.9 cm long while roots from garden soil were only 1.4 cm long. Plantlets from garden soil were taller (7.8 cm) than those from the sand (6.9 cm). All of these plantlets were grown in the potting soil. There were monthly average increases of 40% in plant height, 35% for leaf length and 20% in leaf width. Major/significant findings/ development The preliminary pineapple crown leaf extraction indicated the feasibility of rapid plantlet production using crown leaves with intact stem attachment to the extracted leaves. The pineapple plantlets from pineapple crown could be rooted from sterilized garden soil and coarse beach sand. These plantlets grew well in potting soil.

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