Source: CERMETEK submitted to NRP
THE COMMERCIALIZATION OF A NEW AGRICULTURAL SUBSYSTEM (LEAFMON-S) FOR ONLINE, REALTIME LEAF MONITORING TO DETERMINE CROP HEALTH (STRESS LEVEL) FOR PRECISION IRRIGATION AND WATER CONSERVATION.
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1023268
Grant No.
2020-33610-31947
Cumulative Award Amt.
$98,000.00
Proposal No.
2020-00902
Multistate No.
(N/A)
Project Start Date
Sep 1, 2020
Project End Date
Jul 31, 2021
Grant Year
2020
Program Code
[8.4]- Air, Water and Soils
Recipient Organization
CERMETEK
372-2 TURQUOISE ST
MILPITAS,CA 950355431
Performing Department
(N/A)
Non Technical Summary
This proposal aims to commercialize an innovative non-invassive non-destructiveleaf monitoring system that detects and monitorsplant water status in real-time during the entire crop growing season. The resultant information provides the grower withmeasureable values that assistwith irrigation management to save water. This project specifically addresses the issuesof water use efficiency, which also enhances energy efficiency by reducing the need to pump more water. Thistechnology has the potential to save approximately 30% of the current irrigation water requirements when compared to methodologies presently in practice(present practices referred to as evapotranspiration or ET based irrigation) with major orchard and vineyard crops (almonds, walnuts, and grapes) as well as improvingcrop sustainability efforts.The LeafMon-S product uses various sensors to measure ambient temperature and humidity, leaf temperature and soil mositure. With this data, plant performance models perfected by UC Davis are employed to calculate the plant's water health or CWSI. A grower then applies the minimum required amount of water to ensure that the resulting CWSI value stays within a range specific to each plant type. This methodology providesthe grower with an easy, reliableand repeatable method to manage plant stress while minimzingirrigation water demands and increasing grower efficiency and profitablity.
Animal Health Component
25%
Research Effort Categories
Basic
5%
Applied
25%
Developmental
70%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1020110205025%
1110210301075%
Goals / Objectives
GOALS. To commercialize a new technology resulting in saving of agricultural water resources byincreasing crop water efficiency andreducing water demand while minimizing negative impact on plant health and crop yield. This technology will improve agricultural sustainablity not only in California and dry western United States, but also around the world. The initial basis for this technology was developed at the University of California, Davis, and has been proven in research field tests to reduce water demand by up 30% for major crops like almond, walnuts and grapes when compared to more traditional evapotranspiration (ET) methods.OBJECTIVES.The following are measureable metrics:Reduce leaf monitor retail purchase cost to$200-$250 (USD) per unit.Modify current data interface utilizingan industry standard universal protocol. This will allow connection ofthe leafmonitorto existing comercially available controller units thereby reducing cost of installation/ownership.Change the data interface on the leaf monitor to an SDI-12 serial interface. SDI-12 is commonly available on agricultural data collection equipment. The presence of the SDI-12 interface will allow the grower to install the leaf monitor in locations with existing data collection systems. SDI-12 also allows up to ten sensors to be connected to one data reader. Allowing multiple leaf monitors to be connected to a single data collection device will result in a reduction in the total cost of using the leaf monitor.Improveaccuracy of theprinciple water usage metric Crop Water Stress Index (CWSI) of this new technology.Add a second IR temperature sensor to monitor non-transpiring leaf temperature. Non-transpiring leaf temperature is an essential element in the calculation of CWSI water status. Previous versions of the leaf monitor relied on estimations of non-transpiring leaf temperature. Measuring the non-transpiring leaf temperature will result in more accurate CWSI calculations as indicated by energy balance principles (Kizer, 2t al., 2017 and 2018) and additional research conducted by Professor Upadhyaya's research team at UC Davis.Redesign electrical circuit to reduce cost and add second IR temperature sensor for measuring non-transpiring leaf temperature.Redesign of the electrical circuits is needed to incorporate the universal communications function offered by the SDI-12 protocol. Thisalso permits adoption of the second IR sensor for improved CWSI calculations with the "non- transpiring leaf monitor." While the costs of the electrical circuits are a smaller portion of the overall leaf monitor costs, some savings can be found here to reduce the overall unit costs.Modify electrical circuits to utilize a second IR temperature sensor for monitoring "non- transpiring leaf" conditions.Remove the wind sensor from electrical circuits. This sensor is not needed as long as the sensors in the leaf monitor are adequately protected from wind by the shielding on the leaf holder.Replace the existing RS485 serial interface to the leaf monitor with the more widely adopted SDI-12 interface, See Figure 3. All data from the leaf monitor will be accessible only through the SDI-12 interface. The finished leaf monitor must meet all specifications described in version 1.3 of the SDI-12 serial interface standard.Modify embedded code to incorporate second IR temperature sensor in CWSI calculations and to incorporate an SDI-12 serial interface so that the leaf monitor can be used with most any commercial data collector.Utizlizing a technique commonly referred to as "peripheral processing", the leaf monitor'smicrocontroller will use it's sensor suitedata to calculate CWSI. The microcontroller will then transfer the calculated CWSI and associated sensor data to any connected data reader over the industry standard SDI-12 communications link.Improve monitor leaf holdertosimplifymonitor installation procedures, minimize leaf damage and reduce cost of manufacture. Redesign of the leaf holding hardware is essential for reducing the unit cost of the leaf monitor and allowing the leaf to be more easily installed and more reliably held during the growth cycleModify the leaf holder hardware to simplify installation of the leaf into the leaf monitor. The leaf holder in the prototype leaf monitor utilized two plates to hold the leaf in place. When these plates were opened they provided only a small opening in which to place the leaf. As a result it was difficult to install the leaf without damaging it. Sometimes the damage would not be recognized at the time of installation and a new leaf would need to be installed after a couple of weeks because of the unseen damage.The leaf holder must open a minimum of ninety degrees for installation of the leaf.Reduce the number of components used to construct the leaf holder. Eliminate separate top and bottom plates and combine the leaf dome with the wind blocker.Allow electrical circuits to be surface mounted directly to the mounting plate to reduce assembly costs.Provide holder for second "non-transpiring leaf" targeted by the newly added IR temperature sensor.Define the opacity of the leaf dome at 50% for uniform crop water stress results regardless of sun angle or leaf cover.Add vents to leaf dome to permit heat build-up within the leaf monitor. Note this may require a change in the shape of the leaf monitor to prevent water from entering the leaf dome.Replace the plastic/metal case covering the electronics in the original design with conformal coating. This will provide more durable protection for the circuitry and reduce assembly costs.Modify existing software. New code will not result in any significant cost savings, but is needed to utilize the "non-transpiring-leaf" temperature monitor and tosupportincorporation ofthe SDI-12 interface into the leaf monitor.?Modify the embedded code to use the second IR temperature sensor for monitoring "non-transpiring leaf" conditions.Incorporate the SDI-12 interface for communications from the leaf monitor. The finished leaf monitor must meet all specifications described in version 1.3 of the SDI- 12 serial interface standard.
Project Methods
Product Development and Distribution. This project will be conducted in the following phases.Phase 1 - This phase encompases the various technical improvements and software enhancements required to meet product specific performance goals and objectives. Specifically, efforts needed to provide a cost-effective, highly reliable tool for live measurement of plant transpiration utilizing an improved leaf mount, simplied leaf holding apparatus, and inclusion of a non-transpiring leaf to provide a stable reference. Upon completion of Phase 1, the commercial leaf monitor will be available for widespread field testing by a variety of data collectors and collaborators.Phase 2 - This phase will involve the sale and installation of the initial productionof commercialized products to a few select growers who have already used the basic prototype LeafMon units in the field and are anxious to proliferate the LeafMon-S units in their fields. The initial growers which show commercial promise are those growing walnuts, almonds, grapes and pistachios in California. They would verify the usefulness, robustness, and effectiveness of the LeafMon-S.Phase 3 - In this phase, Cermetek will engage with an identified group of a half dozen independent irrigation communication system manufacturers (i.e., controller manufactures) who would find that our LeafMon-S would enhance their product lines and increase their sales. The types of companies that have already been engaged and include: Ranch Systems, Irrometer, Waterbit, Netafim, etc. These manufacturers already produce standard SDI-12 interface controllers that would accept and operate directly with the plug-and-play LeafMon-S.Phase 4 - Lastly, during phase 4 Cermetek will sell the LeafMon-S units to dealers and distributors of irrigation equipment and various forms of agricultural related communication systems from companies mentioned in Phase 3. Each of the dealer-distributors would then sell and support complete systems which would include the LeafMon-S. The simplified design and installation improvements in the LeafMon-S will translate to minimal support service demands from custers thereby fromthe dealers therebyminimizing dealer-distributorcost-of-sales.Evaluation, Efforts and Verification. At all stages of development, improvement, evaluation and performance verfication, LeafMon-S sensor parametric data and the resulting CWSI values will collected. Parameters collected:Ambient Temperature.Ambient Humidity.Monitored (i.e., live) leaf temperature.Reference (i.e., dead) leaf temperature.Soil Moisture.CWSI.Time and Date stamp.User feedback will be analyzed to ensure that the maximum grower benefit (i.e., expended cost and ROI) isattained. Parameters monitored:Initial cost of implementation.Ongoing cost of ownership.Preferred monitioring/reporting periodicity vs crop seasonal growth progression.Realized reduced cost of operations due to LeafMon-S.Recommendations for LeafMon-S unit deployment and positioning with field acreage.Desired performance and feature enhancements.User data and feedback will be used to tune the CWSI calculations and to continually improve the LeafMon-S. Asadditional crop types are monitored and data submitted, the knowledge base detailing acceptable ranges of CWSI valuesthroughout the growing season for the specific crop will be added to the CWSI Crop Management database. This database will be available to all LeafMon-S users.The primary metrics for successful implelemtation will be:Irrigation water use reduction.Crop yield impactdue to reduced water usage.Improved Crop Water Efficiency.Overall plant health, disease resilience, etc, as water use is reduced.Production cost savings.It is anticipated that as the LeafMon-S unit gains popularityand becomes more widely deployed, theCWSI CropManagement database will become more complete and will be able to provide enhanced management data, such as elevation dependencies.

Progress 09/01/20 to 07/31/21

Outputs
Target Audience:Broadly speaking, all growers utilizing significant deficit irrigation methods to reduce crop losses due to climate change are potential users as initial field data reported suggests that the VPD, DR and PWSI metrics generated by analysis of the LeafMon-S measured parameters are potentially more indicative of plant performance than the traditional methods evaluated. A highly targeted audience for the LeafMon-S product are growers with existing SDI-12 PLC data logger installations. In these installations, the LeafMon-S would serve as either the primary monitoring element and/or as a secondary addon element to the grower's deployed system. Changes/Problems:The CWSI (Crop water Stress Index) metric, employed by the LeafMon (non-SDI-12) unit to monitor plant water stress, relies on sophisticated mathematical regression techniques to determine the fully saturated baseline constants required to calculate CWSI. The necessary regression algorithms currently reside on Cermetek's AquaMonRSVP web server. Unfortunately, the available software libraries for the LeafMon uP upon which the LeafMon-S is based does not contain the required mathematical functions. Additionally, the CWSI metric proved to be too memory intensive for the LeafMon-S model. It was also determined that the internal uP clock function lacked the accuracy/timing stability needed to track the required parametric measurements. And lastly, ongoing analysis of field data and input from our collaborators suggested that an additional refinement to the CWSI metric more closely aligning it with the plant's environment was thought to be more representative of the plant's water stress status. For these reasons: The PWSI (Plant Water Stress Index) metric was created to replace the CWSI (Crop Water Stress Index) metric. PWSI calculations were performed on the grower's Windows© platform rather than on the LeafMon-S unit. The Windows© platform based LeafMon Analysis 1.0 application and the EXCEL© based macro LeafMon_EXCEL_1.0 were created to facility Grower analysis of the LeafMon-S measured parameters. Wider ranging impact discovery via field data and feedback from those collaborators investigating water stress irrigation techniques (≤70% ET) with no crop yield reduction, suggested that a plant performance metric representing plant photosynthesis behavior would be more indicative of the plant's behavior than a water stress metric. A number of empirical algorithms utilizing DR (Dynamic ratio) and DT (Differential Temperature) were conceived and investigated (see Figure 4) in an effort to formulate a photosynthesis metric. These methodologies showed promise, but more field investigative/data corroboration is required to determine a viable photosynthesis metric. What opportunities for training and professional development has the project provided?Dr Andre Daccache, Assistant Professor, Biological and Agricultural Engineering Department, UC Davis has provided access to almond groves thereby providing an opportunity for field installation of LeafMon-S data collection sites in his research activities. Later he has become an active collaborator for LeafMon-S data collection and analysis. Dr. Daccache is now using the LeafMon-S system as a teaching aid in his graduate class (EBS241: Precision Irrigation Management) at the UC Davis Campus, Davis, CA. Specifically, Dr Daccache's EBS241 class has 15 graduate students installing the LeafMon-S units on young walnut orchards. They collect and analyze the LeafMon-S measured parameters as a component of their weekly course assignments. Further, Dr. Daccache demonstrated Cermetek's LeafMon-S unit as well as other commonly used industry data collection systems to approximately 30 invited FMD guests from local farming and support communities at the Kearney Field Day seminar on 23 August 2021. This event was held at the UC Davis Parlier facility. Mr. Mohammad Emami, UC Davis Visiting Research Scholar and PhD candidate, has received LeafMon-S installation and Data Logger interface training (Campbell Scientific Model CR300 and CR800). Mr. Emami served as a LeafMon-S data collection and analysis coordinator for our collaboration with the Button and Turkovich farm in Winters, CA where we monitored walnut trees during the entire growing season. Analysis was shared and tree performances based on actual irrigation practices employed were shared with growers and collaborators on a regular basis. How have the results been disseminated to communities of interest?LeafMon data measurements were collected, and results of calculated metrics shared with the following collaborators, with modifications and enhancements made to the LeafMon-S system incorporating collaborator feedback: Mr. James Nichols, Nichols Farms, Hanford, CA. Mr. Tony Turkovich, Button and Turkovich Farms, Winters, CA Mr. Joseph Gallegos, Umida AG, Fresno, CA, a climate smart irrigation integrator. Four field locations in Phase 1 (listed below), Twenty-Five locations in Phase 2: Center for Irrigation Technology, California State University Fresno (Fresno State). Department of Engineering, University of California Riverside (UCR). Donny Hicks Farm, Turlock, CA. Ramon Vargas Agriculture, Terra Bella, CA. Dr Andres Daccache, Biological and Agricultural Engineering Department, UC Davis on a project at the Kearney Research Facility in Parlier, CA. Umida AG was awarded an NSF SBIR Phase I Grant (# 2051966) on 05/01/2021 to develop a moisture plume simulator to assist growers with evaluation of the potential benefits of the Umida AG's Aquifer Pipe product for their specific soil type(s). Key research groups assisting Umida AG are California State University Fresno (Fresno State), Center for Irrigation Technology and University of California Riverside (UCR), Department of Engineering. LeafMon-S units, coupled with GroPoint Data Loggers, are among several industry systems being evaluated by Umida AG for potential deployment in field data collection and crop monitoring applications in support of commercially deployed Umida AG deep Subsurface Capillary Irrigation Systems. Umida AG has signed a tentative agreement to deploy Phase 2 Beta versions across 25 demonstration farms in California and Arizona. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? 1. Reduced Grower cost of ownership of the basic LeafMon-S Monitor System to $250 (USD) retail purchase price. An additional annual subscription fee of $59 (USD) is required if the Grower chooses to utilize Cermetek's AquaMonRSVP website to analyze, display and archive the LeafMon-S measured data and associated calculated metrics. 2. Successfully modified the existing LeafMon-S hardware and software to be compliant to the industry standard SDI-12 Serial Digital Interface Version 1.3. 1. SDI-12 Version 1.3 compliance verified by third party verification service provider. 2. LeafMon unit successfully connected and provided data with the following third-party Data Loggers: a. GroPoint Model GP-BTDL. b. Campbell Scientific Model CR300. c. Campbell Scientific Model CR800. d. Campbell Scientific Model 1000X. e. EzeSystem Model ezeio. 3. Improved accuracy of Crop Water Stress Index (CWSI) metric by replacing/upgrading hardware and software and by replacing the CWSI metric with the improved Plant Water Stress Index (PWSI) metric. 1. Redesigned electrical circuits to include second IR Sensor and SDI-12 Interface electronics. 2. Removed wind sensor electronics and replaced obsolete components. 3. Replaced RS485 interface with SDI-12 interface electronics. 4. Modified the embedded LeafMon unit code to incorporate second IR sensor and SDI-12 version 1.3 protocol. 5. Improve Accuracy of Crop Monitoring CWSI Metric. Upon detailed review, analysis and comparison of the Crop Water Stress Index (CWSI) metric vs field plant performance provided by user field installations, and coupled with user feedback, it was determined that a new metric, Plant Water Status Index (PWSI) - which is closely related to the more commonly used CWSI metric - can be derived from the data provided by the LeafMon-S monitor. The PWSI metric is the result of LeafMon-S data analysis and user collaboration. It is based on the underlying principles of the CWSI metric, and takes into account the following plant measurements, ambient environmental conditions and additional intermediate metrics: VPD. Vapor Pressure Deficit. A metric that is indicative of atmospheric influence on the plant's transpiration. DT. Differential Temperature. DT = (Dry Leaf Temperature) - (Live Leaf temperature.) DR. Dynamic Ratio. DR = DT/VPD. PAR. Photosynthetically Active Radiation. A metric that corresponds to the amount of light available for photosynthesis by the plant. PWSI. Plant Water Status Index. PWSI = (Average Daily DR)/(Saturated Average Daily DR or DRsat). Where: Average Daily DR. Average DR value over the plant daily activity time range of 8:00AM to 10:00PM (inclusive). This is the time during which the stomata may be open and photosynthesis may be occurring. Saturated Average Daily DR or DRsat. This value is the average DR over the plant activity time range of 8:00AM to 10:00PM (inclusive) for the case when the plant is fully saturated. This typically occurs subsequent to a watering event (about 24hrs) during which the plant is normally free from water stress, especially on those days when VPD levels are low. This information is readily observable when the DT vs time and VPD vs time plots between 8 AM to 10 PM are constructed. It should be noted that the LeafMon-S monitor provides an extensive and wide breadth of data, thus providing the extremely valuable information used to compute the intermediate metrics listed above as well as assisting with alternate crop water status analysis. User feedback indicated that PWSI can be a very useful metric if DR is "normalized" or somehow adjusted for the influence of the plant's response to a watering event, in much the same fashion as CWSI is adjusted using a saturation baseline. PWSI normalization has the effect of limiting the range of PWSI to 0.0-1.0 (inclusive). This approach is much more in line with the CWSI metric. Consequently, although the CWSI and PWSI metric computation procedures are slightly different, they are related to each other by the following relationship: CWSI = 1-PWSI or PWSI = 1-CWSI The plant water stress ranges as indicated by the PWSI metric for the case of almonds, pistachios and walnuts: ALMOND and PISTACHIOS WALNUTS PWSI Range Plant Water Stress PWSI Range Plant Water Stress 1.0-0.75 No to Low Stress 1.00-0.80 No to Low Stress 0.75-0.5 Moderate Stress 0.80-0.60 Moderate Stress <0.50 Severe Stress <0.60 Severe Stress In field studies performed to date, the PWSI appears to track the plant's behavior to water status consistently. See the plot below indicating an increase in PWSI. This is actual field data. 4. Improved the leaf holder of the monitor to simplify monitor installation procedures, minimize leaf damage and reduce cost of manufacturing. a. Reduced the number and complexity of molded components. b. Eliminated the separate top and bottom plates and attached electrical circuits directly to the mounting plate c. Modified leaf holder to open a minimum of ninety degrees for installation of the Dry and Live leaves. d. Combined the leaf dome with the wind blocker and added venting to leaf dome to reduce potential heat build-up e. Added holder for Dry Leaf IR temperature sensor. f. Changed the shape of the leaf monitor enclosure to reduce water entry into the leaf dome. g. Replaced the plastic/metal case covering the electronics in the original design with a conformal coating. 5. The LeafMon-S unit is a low cost, low power system providing the grower with continuous actionable plant water stress metrics. All sensor measurements are initiated by the Data Logger per the SDI-12 Version 1.3 protocol and subsequently recorded in the Data Logger's cache memory: 1. Ambient temperature. 2. IR1 Temperature of Live Leaf. 3. IR1 Internal Temperature of the IR Live Leaf Sensor. 4. IR2 Temperature of Dry Leaf. 5. IR2 Internal Temperature of the IR Dry Leaf Sensor. 6. Relative Humidity. 7. Full Spectrum PAR Light to digital ADC count CH0. 8. Temperature only IR Light to digital ADC count CH1. 9. ADC0 voltage. Reserved for Grower customization. Range 0-5 DC V. If not utilized, reported as 0 by the LeafMon-S unit. The sensors listed in bold above (1, 2, 4, 6, and 7) are critical for developing plant water status metrics. The remaining sensors are used to verify proper sensor performance and to monitor overall LeafMon-S sensor suite health. ADC0 is a standard 0-5V DC input available to the Grower for customization/personalization of the LeafMon-S sensor suite. 6. The following LeafMon System related documents were developed, modified and/or updated to reflect current installation directions, application procedures and usage practices for the LeafMon-S unit: 1. 650-5322 LeafMon-S Users Guide Rev D. This document discusses operation of the LeafMon-S, and provides the user with details concerning the various LeafMon-S measured parameters, the significance of the calculated metrics and suggestions for crop water management utilizing the LeafMon-S metrics. 2. 650-5324 LeafMon-S Design Guide Rev J. A detailed description of the implemented SDI-12 Version 1.3 commands, how to access the LeafMon-S measurements using said SDI-12 commands, a mapping of the LeafMon-S data stream and other associated tips and suggestions for effective use of the LeafMon-S unit. 3. 650-5326 LeafMon-S Windows Application Rev B. This is a tutorial for the installation and use of the LeafMon-S data analysis Windows Platform Application. 4. 650-5327 LeafMon-S EXCEL Macro Rev B. This is a tutorial for the installation and use of the LeafMon-S Microsoft EXCEL Macro for LeafMon-S data analysis.

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