Progress 05/06/20 to 09/30/21
Outputs
Target Audience:Scientists and bioengineers ininterested in increasing plant oil production or controling plant oil fatty acid composition for nutrition, bio-fuels, or industrial chemicals. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?One graduate student and one postdoc have been trained in experimentation, data analysis, and manuscript writting. How have the results been disseminated to communities of interest?Through two journal articles and one book chapter. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
My lab joined this multi-state group in 2019 and thus was involved during the last two years of the project. Our work has focused on understanding the metabolic pathways which control the flux of fatty acids through the lipid metabolic network into membrane lipids and seed oils. In particular understanding the genes that can be manipulated to increase plant oil production, and/or optimize plant oil fatty acid composition for increased nutritional value or as better feedstocks for the bio-fuel or the industrial chemical industry. Recent work utilizing in vivo isotopic tracing has characterized lipid fluxes in Physaria fendleri, a burgeoning crop species that naturally accumulates unusual fatty acids with valuable industrial applications. Progress to date indicates that P. fendleri utilizes a previously uncharacterized triacylglycerol remodeling process to modify the seed oil fatty acid composition after initial synthesis (Bates & Bhandari, 2021). Further characterization of the gene products that control triacylglycerol remodeling will provide new tools to control plant oil fatty acid compositions. Our group has also focused on the control of fatty acid synthesis and total oil accumulation through a collaborative NSF-Plant Genome Research funded project with fellow "Lipids of Crops" Hatch/Multi-state NC-1203 members Jay Thelen (Univ. Missouri), Abe Koo (Univ. Missouri), and Doug Allen (USDA-ARS/ Donald Danforth Plant Science Center). We demonstrated that acetyl-CoA carboxylase (the first committed step of fatty acid synthesis) is partially regulated by pH changes of the light-dark cycle through interaction of BADC proteins with the acetyl-CoA carboxylase subunit BCCP. Mutation of BADC proteins can increase seed oil in a light/dark cycle dependent manner (Ye et al. 2020). Additionally, we analyzed the flux of newly synthesized fatty acids into Camelina sativa seed membrane lipids and oil, within plants overexpressing the pea acetyl-CoA carboxylase a-CT subunit produced by the Koo lab. These plants demonstrate both increased flux of fatty acids into seed oil, and total seed oil accumulation and a manuscript on these results is currently in preparation.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Bhandari S, Bates PD. 2021. Triacylglycerol remodeling in Physaria fendleri indicates oil accumulation is dynamic and not a metabolic endpoint. Plant Physiol 187, 799-815. DOI: 10.1093/plphys/kiab294
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Ye Y, Fulcher YG, Sliman DJ 2nd, Day MT, Schroeder MJ, Koppisetti RK, Bates PD, Thelen JJ, Van Doren SR. (2020) The BADC and BCCP subunits of chloroplast acetyl-CoA carboxylase sense the pH changes of the light-dark cycle. J Biol Chem. 295:9901-9916.
- Type:
Book Chapters
Status:
Accepted
Year Published:
2021
Citation:
Bates PD. 2021. The plant lipid metabolic network for assembly of diverse triacylglycerol molecular species. Advances in Botanical Research: Academic Press. https://doi.org/10.1016/bs.abr.2021.07.003
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Progress 05/06/20 to 09/30/20
Outputs
Target Audience:Scientists interested in lipid metabolism with a the publication: Kotapati H and Bates PD. Analysis of Isotopically-labeled Monogalactosyldiacylglycerol Molecular Species from [14C]Acetate-Labeled Tobacco Leaves. Bio-Protocol. (Accepted) Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project involved the training of one postdoctoral scientist who developed the lipid analysis method and one undergraduate researcher who assisted in the research. How have the results been disseminated to communities of interest?With one accepted publication. What do you plan to do during the next reporting period to accomplish the goals?Obj. 1. During the next period we will further optimize methods for analysis of isotopic labeling of lipid metabolism in various plant tissues. Obj. 2. During the next period we will evaluate the control of lipid metabolic flux into oils of various oil accumulating plant tissues. This research will provide an enhanced understanding of the metabolic pathways which lead to valuable plant oils, and will enable further breeding or engineering of plant oils for food, feed, chemcicals and biofuels.
Impacts
What was accomplished under these goals?
Our recent publication during this report period applies directly to goal 1, as we developed new methods for the analysis of isotopic labeling ofleaf galactolipid lipid metabolism. Galactolipids are essentail to the chloroplast thylakoid membranes which hold the photosystems essential for photosynthesis.This will improve the analysis of plant lipid metabolism in future studies to understand the dynamics of cellular lipid metabolism. In addition these methods will allow us to further prob the affects of adverse environmental conditions on the
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2020
Citation:
Kotapati H and Bates PD. Analysis of Isotopically-labeled Monogalactosyldiacylglycerol Molecular Species from [14C]Acetate-Labeled Tobacco Leaves. Bio-Protocol. (Accepted)
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