Source: UNIVERSITY OF FLORIDA submitted to NRP
HEALTH AND DISEASE MANAGEMENT IN FLORIDA AQUACULTURE
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1022388
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Mar 5, 2020
Project End Date
Mar 1, 2025
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF FLORIDA
G022 MCCARTY HALL
GAINESVILLE,FL 32611
Performing Department
Forest Resources and Conservation
Non Technical Summary
Florida's aquaculture industries are complex because many different species are raised in many different ways. In addition, many of these, including those raised for the aquarium industry and those purchased for grow out at other farms, are sold live, and therefore must also be in the best of health for shipment and ultimate sale at distant locations. Aquaculture health is critical for the organisms and for producers' financial success. However, good health is also important to minimize environmental impact and ensure human safety. Annual losses to the industry caused by disease have been estimated to be $6-10 million. Ultimately, better understanding will improve Florida and the U.S.'s competitiveness in a global market. Diseases in aquaculture are caused by several factors: environmental influences, infectious disease agents including new diseases, management protocols, and the organism's own ability to stay healthy. Understanding these factors is necessary to better prevent and manage disease. When disease does occur, producers also need as many legal, safe and effective options as possible to reduce losses. A better understanding of health management will help reduce losses and increase efficiency and profits. Knowledge needed includes: baseline information on healthy and diseased animals; a better understanding of emerging or more established diseases at all life-stages during production and shipment; and how and why differences in populations of fish affect their health. Better methods for treating sick aquatic animals are also needed. Legal options are very limited, and for some common diseases, no legal drugs are currently available. Similarly, the use of vaccines and other preventative methods are still limited in aquaculture but can help further reduce the need for drugs and chemicals.
Animal Health Component
100%
Research Effort Categories
Basic
0%
Applied
100%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
31137991160100%
Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3799 - Cultured aquatic animals, general/other;

Field Of Science
1160 - Pathology;
Goals / Objectives
Florida's aquaculture industries are complex with regard to species cultured, systems, and other health-related industry challenges. Losses caused by disease in Florida's tropical fish industry have been estimated at $6-10 million annually. Disease in aquaculture results from adverse interactions between the animal host and the environment, and is ultimately determined by the effectiveness of the animal's response to both non-infectious and infectious stressors (Brown 1993; Stoskopf 1993; Iwama et al. 1997; Noga, 2010). Disease outbreaks often result from practices aimed at maximizing efficiency and from environmental factors that can be difficult to control in outdoor systems (Noga, 2010). Hundreds of aquacultured species, complex movement of animals throughout the industry at all levels, differences in production practices, established and emerging diseases and disease diagnostics (Yanong 2003; Noga 2010; Soto et al. 2012), microflora interactions in systems (Yanong et al. 2010) and limited drug and biologic options make disease prevention and management in Florida's aquaculture industries a challenge (Russo and Yanong 2009; Noga 2010). To best manage the health of the numerous aquatic species cultured in Florida, baseline health parameters for clinically normal and diseased animals are required. This includes evaluation and determination of health parameters and characterization of emerging or more established diseases, disease progression, and epidemiology (including risk factors). Studies evaluating health during all life stages (including reproduction), as well as general production and shipping practices, are also important to the industry from an economic and health perspective (Iwama et al 1997). In addition, genetic make-up, i.e., strain variations, have been shown to enhance resistance to disease. Nutrition also plays an important role in fish health (Yanong 1999; Yanong 2001a and 2001b). Drugs, chemicals, and biologics are important fish health management tools for both increased production, reproduction, disease prevention, and disease management (Brown 1993, Stoskopf 1993; Crosby et al. 2005). Pharmaceutical companies have had little economic incentive to apply for drug approvals for ornamental fish, although recent legislation (the Minor Use Minor Species Animal Health Act of 2004) adds more innovative and cost-effective options including "The Index of Legally Marketed Unapproved New Animal Drugs for Minor Species" (the "Index"). The purpose of the INAD program is to allow limited use of drugs for field and clinical research, the data of which will be used to support New Animal Drug Application (NADA) approval or "Index" designation ("Minor Use Minor Species Index of Legally Marketed, Unapproved Drugs"), which allows for legal access of several important aquaculture drugs. Biologics such as vaccines can be cost-effective, but have not been used routinely in Florida's industry. Drug research must include determination of proper dose and dose interval, evaluation of target animal and user safety, efficacy against the target organism(s), and cost effectiveness (Trushenski et al. 2013).
Project Methods
The complexities of Florida's aquaculture industries' health needs necessitate a multi-pronged approach. Health of populations under natural, industry standard, or controlled experimental conditions will be evaluated using one or more of the following: detailed management, transport, and environmental history; water quality and system evaluation; behavioral observations, external exam and general morphometrics; necropsy, blood clinical chemistry and hematology, histology, microbiology/virology, electron microscopy, radiography and other imaging methods, and molecular methods. Emerging or established diseases will be characterized through diagnostics of clinical cases, i.e., examinations from field or production facilities, sent to our disease diagnostic lab, and correlative studies of these clinical cases (Yanong, et al. 1998). Experimental or clinical trials will be used to examine various management and therapeutic approaches, including water and system manipulation, genetic/strain variations, nutrition, use of probiotics, immunostimulants, drugs, chemicals, and biologics, all to determine effectiveness for more efficient production, therapy, or survival on farms. Field trials will be used to evaluate onsite effectiveness of these compounds.

Progress 10/01/20 to 09/30/21

Outputs
Target Audience:Aquatic animal health professionals, producers, and students Changes/Problems:Most changes/problems were due to COVID-19, which shifted sampling and data analyses for the probiotics study. We were able to complete sample collection and are near completion of data analysis. What opportunities for training and professional development has the project provided?A graduate student at UF has been working on major portions of the probiotics study - includingsample collection, processing, and data analysis. How have the results been disseminated to communities of interest?Data and implications have been provided through outreach to producers at producer advisory meetings, by phone, and through on-farm visits. More outreach is planned. What do you plan to do during the next reporting period to accomplish the goals?Although most of the probiotics data analysis has been completed, some additional samples (not originally included in the proposal) are being analyzed and will provide additional useful data. As soon as this work is completed, a manuscript will be completed for journal publication, and additional outreach (meetings, publications) will be planned.

Impacts
What was accomplished under these goals? Through UF/IFAS/my portion of the USDA NIFA SRAC grant Increasing Understanding of and Developing Management Strategies for Edwardsiella ictaluri in Ornamental Fish our more recent probiotics pond research has provided data which has been analyzed and summarized in second year report for SRAC,which, to our knowledge, is the first freshwater aquarium fish production probiotics pond study in the U.S. Our data provide insights into the ability of probiotics to alter microbiome based on dose/application. In addition, we are near completion of our draft manuscript for submission for earlier work from this same grant on pharmacokinetics of florfenicol and enrofloxacin in giant danios.Within this reporting period, a presentation was given on the probiotics work and a poster was given on thedrug studiesat theInternational Association of Aquatic Animal Medicine Virtual Conference to aquatic animal health professionals.In addition, we published a paper on a newly described microsporidial disease which has better allowed us to inform our producers on potential management options.Finally, work done providing preliminary data on use of metomidate for euthanasia of aquarium fishes was published and supports the use of this drug for many species.

Publications

  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Lovy J, Yanong RP, Stilwell JM, Waltzek TB, Shelley JP, Pouder DB, Wolf JC, Camus AC. 2021. Tetra disseminated microsporidiosis: a novel disease in ornamental fish caused by Fusasporis stethaprioni n. gen. n. sp. Parasitol Res.120(2):497-514.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Koda, S.A., Subramaniam, K., Waltzek, T.B., Pouder, D.B., Yanong, R.P., Frasca Jr., S., and Popov, V.L. 2021. Complete genome sequences of infectious spleen and kidney necrosis virus isolated from farmed albino rainbow sharks Epalzeorhynchos frenatum in the United States. Virus Genes 57, 448452
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Brammer-Robbins L, E. K. Freeman, A. S. Kanarek, J. H. Bisesi, E. J. Cassiano, Q. M. Tuckett, R.P.E. Yanong, C. J. Martyniuk. 2021. Evaluating Probiotic Treatments in the Ornamental Fish Aquaculture Industry: Implications for Managing Edwardsiella ictaluri Outbreaks and Fish Microbiota. International Association for Aquatic Animal Medicine Virtual Conference. May 25-26.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2021 Citation: Vorbach B.S., J. Bulitta, J. Zhou, Y. Lang, R.P.E. Yanong. 2021. Pharmacokinetics and pharmacodynamics of enrofloxacin and florfenicol in the giant danio (Devario aequipinnatus) following oral and bath administration. International Association for Aquatic Animal Medicine Virtual Conference. May 25-26.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Yanong, R.P.E. 2021. Preliminary Investigations into Use of Metomidate for Euthanasia of Ornamental Fishes. J Aquat Anim Health.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Rhody, N.R., Stacy, N.I., Hernandez, J.A., Patrick, G., Resley, M.J. and Yanong, R.P. (2021), Blood Analyte Changes of Wild-Caught Adult Almaco Jack in Response to Acclimation to Recirculating Aquaculture Systems and Hyposalinity Treatment. J Aquat Anim Health, 33: 77-83.
  • Type: Journal Articles Status: Published Year Published: 2021 Citation: Wright, S.E., Stacy, N.I., Yanong, R.P., Juhl, R.N. and Lewbart, G.A. (2021), Hematology and Biochemistry Panel Reference Intervals for Captive Saddleback Clownfish Amphiprion polymnus and Tomato Clownfish A. frenatus. J. Aquat. Anim. Health, 33: 3-16


Progress 03/05/20 to 09/30/20

Outputs
Target Audience: Nothing Reported Changes/Problems:Major change/problems, were due to COVID-19, which curtailed some of the timing of our sampling, specifically for the USDA NIFA SRAC E. ictaluri probiotics study. Regardless, we believe we have used the flexibility we can to obtaingood sample data at relevant timepoints. What opportunities for training and professional development has the project provided?A graduate student at UF has been working on major portions of the probiotic study (samples and data currently being analyzed). How have the results been disseminated to communities of interest?Data and implications has been provided through outreach to producers by phone and on-farm visits. More outreach is planned. What do you plan to do during the next reporting period to accomplish the goals?Data analysis for our USDA NIFA SRAC probiotics study will be completed and manuscript written for journal publication. We also plan to extend this information to producers and to fish health professionals at various industry and professional meetings, and through on-farm /site visits. The completed USDA NIFA SRAC UF/IFAS portion of the pharmacokinetics /pharmacodynamics (antibiotic/drug) studies will be presented at professional meetings and through producer outreach (meetings, extension publications).

Impacts
What was accomplished under these goals? Through UF/IFAS/my portion of the USDA NIFA SRAC grant Increasing Understanding of and Developing Management Strategies for Edwardsiella ictaluri in Ornamental Fish our research has provided data which has been analyzed and summarized in an interim report for SRAC, on pharmacokinetics/pharmacodynamics for enrofloxacin and florfenicol in Giant Danios as a proxy for zebra danios, which will better inform how to manage this bacterial disease with antibiotics and correct dosing/administration. We are finalizing draft manuscript for submission and also have submitted a poster abstract for the International Association of Aquatic Animal Medicine Virtual Conference and are awaiting acceptance. As part of this grant, we have also just completed data collection for our probiotics study, which is examining the effectiveness of two different types of probiotics in the field on two farms at reducing the bacterial load of E. ictaluri on the microbiome of the fish's skin, intestinal tract, and in the pond water, as well as possible effects on morphometrics and harvest numbers. This data is currently being analyzed. In addition work on specific diseases seen in the ornamental fish industry including megalocytivirus and a new, emerging microsporidia (work ongoing) have provided additional information that will help inform us and producers on how to better manage these diseases. Finally, as mentioned, in conjunction with other colleagues with NIFA projects, we have assisted in work looking at larval diets and their potential utility and effect on production and survival in the ornamental fish industry.

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Elizabeth C. Scherbatskoy, Kuttichantran Subramaniam, Lowia Al-Hussinee, Kamonchai Imnoi, Patrick M. Thompson, Deborah B. Pouder, Roy P.E. Yanong, Vsevolod L. Popov, Terry Fei Fan Ng, Thomas B. Waltzek. 2020.Characterization of a novel picornavirus isolated from moribund aquacultured clownfish. Journal of General Virology 101 (7).
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Taylor N.Lipscomb, Roy P.Yanong, Shane W.Ramee, Matthew A.DiMaggio. 2020.Histological, histochemical and biochemical characterization of larval digestive system ontogeny in black tetra Gymnocorymbus ternetzi to inform aquaculture weaning protocols. Aquaculture 520.