Source: UNIV OF ARKANSAS submitted to NRP
DISSOLUTION, TOXICITY AND TRANSPORT OF NANOPARTICLES IN AGRICULTURAL ECOSYSTEMS: IMPACTS OF PARTICLE SIZE, SURFACE COATING AND AGING
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
1022047
Grant No.
2020-38821-31081
Cumulative Award Amt.
$499,966.00
Proposal No.
2019-03229
Multistate No.
(N/A)
Project Start Date
May 15, 2020
Project End Date
May 14, 2025
Grant Year
2020
Program Code
[EQ]- Research Project
Recipient Organization
UNIV OF ARKANSAS
(N/A)
PINE BLUFF,AR 71601
Performing Department
Agriculture
Non Technical Summary
Nano-enabled agricultural fertilizers and pesticides have a greater potential for direct, massive introduction of manmade nanomaterials into the environment than any other use of nanoparticles. However, our current understanding of nanomaterial fate and transport in the agriculture environment remains limited. The goal of this project is develop an interdisciplinary research program to gain insight into the physical, chemical and biological processes that controlled by properties of nanoparticle, such as size, coating and aging. The research is structured around four tasks: 1, determination of nanoparticle dissolution rates as a function of surface size, coating and aging effect; 2, elucidation of key aspects of nanoparticle-plant-soil interactions in rhizosphere soil; 3, identification of the fundamental processes controlling nanoparticles toxicity to soil microbe in agricultural soils; 4, evaluation of the fundamental processes controlling nanoparticles retention in dynamic transport system in agricultural soils. The knowledge gained through this project regarding the risks related to utilizing nano enabled agrochemicals will play a key role in guide nanoparticles safe design, administration, regulation and responsible application. Integrated with plant science, soil science and environmental chemistry programs, successful completion of the proposed project will increase UAPB's research capacity on nano enabled agriculture development for sustainability.
Animal Health Component
40%
Research Effort Categories
Basic
30%
Applied
40%
Developmental
30%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1020110200050%
1024099100050%
Goals / Objectives
The goal of this project is to develop an interdisciplinary research program to expand UAPB's nanotechnology research capacity. The research program is designed to elucidate the potential risks related to the application of nanoparticles (NPs) in agricultural field. In particular, this project will focus on the NP's property (size, surface coating or aging) dependent transformation and toxicity. The proposed project will combine laboratory experiments with mathematical modeling to quantify and predict the effects of particle size and surface coating on the dissolution, toxicity and transport of CuO and ZnO NPs in soils.The research is structured around four objectives:1. Determination of dissolution rate of NPs in soils as a function of size, surface coating, and aging;2. Elucidation of key aspects of NP-plant-soil interactions in rhizosphere soil.3. Identification of the fundamental processes controlling NP toxicity to microbes in agricultural soils.4. Evaluation of the fundamental processes controlling NP retention in dynamic transport systems in agricultural soils.
Project Methods
The dissolution status of the NPs in agricultural ecosystems (i.e. whether they remain in particulate form or dissolve) strongly affects their uptake pathway, toxicity to soil microbial and leachability in soil. Therefore, dissolution process of NPs will be studied to understand the true biological effect of NPs. In depth study will be designed to quantify the influence of time and near-root chemical conditions on dissolution and bioavailability of CuO/ZnO NPs in rhizosphere soil, and to determine the influence of tomato and lettuce during a 28-day plant growth period. Soil enzymes will be studied to understand the effect of NPs on soil health. Lab column transport experiment will be carried out to study the mobility and leachability of NPs in agriculture soil.

Progress 05/15/24 to 05/14/25

Outputs
Target Audience: Agricultural Scientists and Researchers: Specialists focusing on nanotechnology applications in agriculture. Farmers and Agricultural Producers: End-users interested in advanced crop protection and enhancement solutions. Agribusiness Companies: Firms involved in developing, manufacturing, and distributing agricultural chemicals. Policy Makers and Regulatory Authorities: Officials overseeing the safety, regulation, and approval of agricultural inputs. Environmentaland Health Organizations: Groups concerned with the impacts of nano-enabled chemicals on health and the environment. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The project has provided training and professional development opportunities across different levels, including master's students, undergraduate students, and university staff. Participants engaged in cutting-edge research in nanotechnology applied to agriculture, gaining valuable experience in laboratory techniques, data analysis, and environmental risk assessment. This diverse educational approach enhances students' academic and professional skills and fosters a culture of interdisciplinary research and innovation among university staff, contributing to the broader academic community's expertise in sustainable agricultural practices. How have the results been disseminated to communities of interest?The results have been disseminated to communities of interest through paper publications in scholarly journals and presentations at local and national conferences. This approach ensures that the findings reach a broad audience, including researchers, practitioners, and stakeholders in sustainable agriculture and nanotechnology. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Enhanced Understanding of NP Behavior: Identified how CuO and ZnO nanoparticles' properties (size, surface coating) influence their transformation, dissolution, and toxicity in soil, contributing to safer agricultural application. Innovations in Green Synthesis: Demonstrated the efficacy of green-synthesized ZnO nanoparticles using sweet potato leaf extract, offering an eco-friendly alternative to conventional chemical methods. Reduction of Heavy Metal Uptake: Foliar application of ZnO nanoparticles can significantly reduce Cd uptake in lettuce, highlighting the potential for minimizing heavy metal accumulation in crops. Positive Impact on Plant Health: Nanoparticle treatments could enhance plant growth under stress conditions, improve antioxidant activities, and support healthier chlorophyll content, underscoring the benefits of nano-enabled agricultural practices.

Publications


    Progress 05/15/23 to 05/14/24

    Outputs
    Target Audience:1. Agricultural Scientists and Researchers: Specialists focusing on nanotechnology applications in agriculture. 2. Farmers and Agricultural Producers: End-users interested in advanced solutions for crop protection and enhancement. 3. Agribusiness Companies: Firms involved in developing, manufacturing, and distributing agricultural chemicals. 4. Policy Makers and Regulatory Authorities: Officials overseeing the safety, regulation, and approval of agricultural inputs. 5. Environmental and Health Organizations: Groups concerned with the impacts of nano-enabled chemicals on health and the environment. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

    Impacts
    What was accomplished under these goals? Accurately determined the dissolution rates of nanoparticles in soils, revealing crucial size, surface coating, and aging dependencies. Illuminated critical interactions among nanoparticles, plants, and soils within the rhizosphere, enhancing our understanding of nanomaterial behavior in agricultural settings. Identified key processes governing nanoparticle toxicity to soil microbes, contributing to safer agricultural practices. Evaluated and mapped out the retention processes of nanoparticles in dynamic transport systems within agricultural soils, paving the way for improved environmental risk assessments and management strategies.

    Publications

    • Type: Journal Articles Status: Published Year Published: 2024 Citation: Kaushik Adhikari, Anil Timilsina, Hao ChenThe Effect of Size and Surface-Coating of Copper Oxide (CuO) Nanoparticles on Bioavailable Cu and Enzyme Activities in Soil Soil and Environmental Health Journal. Accepted


    Progress 05/15/22 to 05/14/23

    Outputs
    Target Audience: Agricultural Scientists and Researchers: Specialists focusing on nanotechnology applications in agriculture. Farmers and Agricultural Producers: End-users interested in advanced solutions for crop protection and enhancement. Agribusiness Companies: Firms involved in developing, manufacturing, and distributing agricultural chemicals. Policy Makers and Regulatory Authorities: Officials overseeing the safety, regulation, and approval of agricultural inputs. Environmental and Health Organizations: Groups concerned with the impacts of nano-enabled chemicals on health and the environment. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The project has provided opportunities for training and professional development across different levels, including master's students, undergraduate students, and university staff. Participants engaged in cutting-edge research in nanotechnology applied to agriculture, gaining valuable experience in laboratory techniques, data analysis, and environmental risk assessment. This diverse educational approach not only enhances the academic and professional skills of students but also fosters a culture of interdisciplinary research and innovation among university staff, contributing to the broader academic community's expertise in sustainable agricultural practices. How have the results been disseminated to communities of interest?The results have been disseminated to communities of interest through paper publications in scholarly journals and presentations at both local and national conferences. This approach ensures that the findings reach a wide audience, including researchers, practitioners, and stakeholders involved in sustainable agriculture and nanotechnology fields. What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period, the plan is to focus on finalizing the current research findings and exploring ways to effectively advocate these results within relevant communities. Additionally, efforts will be directed towards identifying and pursuing opportunities for further funding to continue and expand the study, ensuring ongoing contribution to the field of nanotechnology in agriculture.

    Impacts
    What was accomplished under these goals? Enhanced Understanding of NP Behavior: Identified how CuO and ZnO nanoparticles' properties (size, surface coating) influence their transformation, dissolution, and toxicity in soil, contributing to safer application in agriculture. Innovations in Green Synthesis: Demonstrated the efficacy of green-synthesized ZnO nanoparticles using sweet potato leaf extract, offering an eco-friendly alternative to conventional chemical methods. Reduction of Heavy Metal Uptake: Showed that foliar application of ZnO nanoparticles can significantly reduce Cd uptake in lettuce, highlighting the potential for minimizing heavy metal accumulation in crops. Positive Impact on Plant Health: Found that nanoparticle treatments could enhance plant growth under stress conditions, improve antioxidant activities, and support healthier chlorophyll content, underscoring the benefits of nano-enabled agricultural practices.

    Publications

    • Type: Journal Articles Status: Published Year Published: 2023 Citation: Anil Timilsina, Kaushik Adhikari, Hao Chen, Foliar application of green synthesized ZnO nanoparticles reduced Cd content in shoot of lettuce, Chemosphere, Volume 338, 2023, 139589, ISSN 0045-6535, https://doi.org/10.1016/j.chemosphere.2023.139589.
    • Type: Journal Articles Status: Accepted Year Published: 2024 Citation: Kaushik Adhikari, Anil Timilsina, Hao ChenThe Effect of Size and Surface-Coating of Copper Oxide (CuO) Nanoparticles on Bioavailable Cu and Enzyme Activities in Soil Soil and Environmental Health Journal. Accepted
    • Type: Theses/Dissertations Status: Accepted Year Published: 2022 Citation: EFFECTS OF GREEN SYNTHESIZED ZNO NANOPARTICLE FOLIAR SPRAY ON Cd UPTAKE IN LETTUCE AND SWEET POTATO


    Progress 05/15/21 to 05/14/22

    Outputs
    Target Audience:There are five key audiences for this research, these are: USDA employees, farmers, local public, external organizations (such as Environmental Protection Agency (EPA), Environment Quality Observatories) and academia. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Two master's student training and one undergraduate student training. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

    Impacts
    What was accomplished under these goals? Our study explored the compatibility of Bio-ZnO NPs foliar spray as micronutrient fertilizer and its role in addressing the Cd accumulation in contaminated soil. We found the sweet potato leaf extract was found to play an important role as stabilizing and capping agent for the green synthesis of ZnO NPs. These green synthesized ZnO NPs were found effective in supplying Zn to lettuce via foliar application. ZnO NPs supplementation through foliage had positive effects on crop growth in Cd contaminated soil and negatively affected shoot Cd content. To comprehend the mechanism of reduced Cd in shoots due to foliar application of ZnO NPs, detailed research on the transport and fixation of these metals in plant systems is required. In addition, future research will look into the effects of ZnO NPs foliar application on root Cd uptake and the relationship between root and shoot Cd and Zn accumulations.

    Publications

    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2021 Citation: EFFECTS OF GREEN-SYNTHESIZED ZnO NPs FOLIAR SPRAY ON Cd ACCUMULATION IN LETTUCE SHOOT Anil Timilsina & Hao Chen 2021 ASA, CSSA, and SSSA annual internaitonal meeting. Salt Lake city.
    • Type: Book Chapters Status: Accepted Year Published: 2021 Citation: Nano- and nanobiosensors for sustainable agriculture Rahul Bhagat, Avinash P. Ingle, Hao Chen


    Progress 05/15/20 to 05/14/21

    Outputs
    Target Audience:The knowledge generated from research involves a wide range of stakeholders, such as scientists, farmers, land managers, advisory services, regulators, educators, students, as well as the general public Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?We have trained one master student and one undergrad on nano-enabled agriculture. They are trained for both experiment design, bench top experiments, and greenhouse experiments. For the master student, the instrumental analysis skills have also been trained. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?We will carry the dissolution and transport experiment for the next year. The lab has fully prepared for the experiments. Two master students are hired to carry these experiments.

    Impacts
    What was accomplished under these goals? We have initiated the research program including hiring both graduate and undergrad students and setting up the lab and greenhouse. Two sets of critical experiments have been done: 1) nanoparticle synthesis and 2) nanoparticle interaction with plants (lettuce and sweet potato). We have one conference presentation about the effect of synthesized nanoparticles used for seed priming. We also have submitted a book chapter about the effect of nanoparticles on plants.

    Publications

    • Type: Book Chapters Status: Awaiting Publication Year Published: 2021 Citation: The Emerging Applications of Zinc-Based Nanoparticles in Plant Growth Promotion Anil Timilsina and Hao Chen
    • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Biosynthesis of ZnO Nanoparticles from Sweet Potato Leaf Extract and Seed Priming Treatment Effects on Carrot Seed Anil Timilsina & Hao Chen ASA, CSSA and SSSA International Annual Meetings (2020)