Source: PENNSYLVANIA STATE UNIVERSITY submitted to NRP
ECOLOGY AND SYSTEMATICS OF FRESHWATER FISHES
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1021995
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Feb 1, 2020
Project End Date
Dec 31, 2024
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
PENNSYLVANIA STATE UNIVERSITY
208 MUELLER LABORATORY
UNIVERSITY PARK,PA 16802
Performing Department
Ecosystem Science & Management
Non Technical Summary
The problems identified on a world-wide scale also impact our local aquatic flora and fauna. The distribution of the fishes within Pennsylvania was initially related to plate tectonics, physiography, and geomorphology of the region. Fenneman (1938) recognized the Coastal Plain, Piedmont, Blue Ridge, Ridge and Valley, and Appalachian Plateau as distinct provinces of the region. It is generally conceded that the Appalachian Mountains evolved in the Taconic (Ordovician), Acadian (Devonian) and Appalachian (primarily Permian) orgogenies. Contemporary thinking (e.g., Judson 1976, Cook et al. 1980) strongly suggests that these three major peaks of mountain building were a direct consequence of the actions of plate tectonics that characterized the Paleozoic from Ordovician through Permian times. Relative to the formation of these barriers to fish dispersal, Schmidt (1986) hypothesized that five disjunct refugia contributed to the fauna of the northern Appalachian region. The two refugia that would have most influenced the fauna within the region that is now Pennsylvania are the Mississippi Valley and the Atlantic Coastal Plain. The Mississippi River basin is hypothesized to be the primary center of origin and dispersal of fishes east of the Rocky Mountains; thus, the high diversity of fishes found in these drainages relative to the Atlantic Coastal Plain is expected. The overflow caused by melting glaciers probably would have been important in the dispersal of fishes from more northern regions into the Susquehanna, Delaware, and Potomac basins of the Atlantic Slope, and caused stream reversal and dispersal of the Great Lakes fauna into the Allegheny and Monongahela rivers, and vice versa. There is no doubt that these events determined the original distribution of fishes with the Commonwealth. More recently, however, anthropogenic influences have greatly altered the current arrangement of fish distribution patterns within Pennsylvania. Such influences include the direct transport of fishes through intentional or accidental introductions and changes in water quality caused by direct input of toxins into our waterways or by landscape alterations, which in turn change the physico-chemical parameters of run-off.The quantification of biodiversity, for the most part, hinges on the number of species present in a particular system. Thus, the concept of species has long influenced the way in which humans view the diversity of life (Ruffing et al. 2002). The goal to define the species category has provoked more discussions and arguments than perhaps any other topic in comparative or evolutionary biology (Eldridge 1995). Darwin (1859) recognized the difficulty in delimiting species when he noted that many forms considered by competent scientists as varieties are ranked as species by other competent researchers.I recently redescribed the Brook Trout (Salvelinus fontinalis) from the type locality on Long Island, New York and designated a neotype, since a holotype was never preserved. My students and I are now working on the systematics of the "Brook Trout" from Pennsylvania and Great Smoky Mountains National Park in Tennessee and North Carolina. Our preliminary conclusions indicate that Brook Trout do not occur in PA, but are in fact other species in Salvelinus.
Animal Health Component
20%
Research Effort Categories
Basic
60%
Applied
20%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
13602101060100%
Goals / Objectives
1) continue to describe species of freshwater fishes;2) use of CT scanner to refine our analysis of morphology of both African and North American fishes and to compare phenotypic plasticity in introduced and native fishes;3) to develop recover plan for endangered fishes and monitor introduced fishes;4) expand the range of the Chesapeake Logperch which is endemic to the Susquehanna River drainage in Pennsylvania and Maryland.
Project Methods
1. Continue to describe species of freshwater fishes. As part of our continuing research in Ohio River basin, we have recently discovered a new species of darter related to Percina caprodes.We have been studying the cichlid fishes of Lake Malawi for the past two decades. The cichlids of Lake Malawi certainly provide a tremendous forum for the discussion of species concepts, given the explosive radiation and diversification within this system. Cichlid fishes represent an outstanding case of explosive evolution and offer extraordinary opportunities to investigate evolutionary processes that have led to such diversity. Rapid speciation within these fishes (Stauffer and McKaye 2001), however, has resulted in a paucity of characters for discriminating among species. Our experiences in Africa and Central America demonstrate that in situ behavioral studies, integrated with morphological and genetic analysis of taxonomic units are vital to determining the specific status and relationships among taxonomic units (Stauffer et al. 1995). Determination of the specific status of local taxonomic units is critical for the development of programs both to conserve and to utilize these fishes for food, tourism, disease control, and scientific investigations.2. In the 1980s, morphometrics experienced a major revolution through the invention of coordinate-based methods, the discovery of the statistical theory of shape. This new morphometric approach has been termed geometric morphometrics as it preserves the geometry of the landmark configurations throughout the analysis and thus permits to represent statistical results as actual shapes or forms (Mitteroecker and Gunz, 2009). In morphometrics, the term shape is used to characterize the geometric properties of an object that are independent of the object's overall size, position, and orientation, whereas the form of an object comprises both its shape and size. Geometric morphometrics is based on landmark coordinates. Shape and shape change are intrinsic to survival and eventually to evolution. In the course of evolution, the cichlid head has adapted to many vital functions. To begin a study of cranial shape variation, we will acquire 3D HRCT scans and collect the 3D coordinates of landmark locations from 3D reconstructions based on bone tissue threshold.3. Develop recovery plans for endangered species and monitor introduced fishes. Objectives of the restoration of the Chesapeake Logperch include: 1) culture 2500 fry for reintroduction, 2) identify suitable habitat in the Susquehanna River Watershed (BCW) to reintroduce these species, 3) mark the cultured fry with unique tags, 4) reintroduce cultured minnows into selected areas, and 5) monitor populations for growth, maintenance, and reproduction; 5) move wild fishes from areas where they are abundant to areas where they have been extirpated; 6) compare the success of reintroduction of cultured versus wild fishes in establishing reproducing populations.

Progress 02/01/20 to 09/30/20

Outputs
Target Audience:Other scientists, especially those working on ecology and systemic biologyof fishes in North America and those scientists at the South African Institute of Aquatic Biodiversity and Lilongwe University of Agriculture and Natural Resources. Fisheries scientists of the Pennsylvania Fish and Boat Commission and National Park Service. Changes/Problems:The cultured Chesapeake Logperch have had a high mortality rate as they transition from larva to juveniles. Water filtration, prophylactic treatment of fungal and bacterial diseases have been implemented. Additionally, we have changed the diet of the larval fishes. To date, these changes have greatly increase survival rates. What opportunities for training and professional development has the project provided?Several students are continuing their graduate degrees working on the projects described above. One student is currently writing his MS thesis, has graduated from Penn State Law School, and will receive an MS as part of a joint degree in Wildlife and Fisheries Science program in Ecosystem Science and Management. How have the results been disseminated to communities of interest?My students and I have presented papers at scientific meetings. I gave seminars at the South African Institute of Aquatic Biodiversiy highlighting our work on Pennsylvanian fishes. I was contacted bythe community surrounding Lake Harmony, PA for advice in increasing fishing opportunities in a local lake. What do you plan to do during the next reporting period to accomplish the goals?My students and I will continue thestudy of cranial shape variation; and,we will acquire 3D High Resolution Computer Tomography (HRCT) scans and collect the 3D coordinates of landmark locations from 3D reconstructions based on bone tissue threshold. We will continue our efforts for the recovery of the endangered Chesapeake Logperch. To date my students and I have cultured some 6000 larva for reintroduction. We will continue our efforts to identify suitable habitat in the Susquehanna River Watershed (BCW) to reintroduce these species;mark the cultured fry with unique tags;reintroduce cultured minnows into selected areas; monitor populations for growth, maintenance, and reproduction;move wild fishes from areas where they are abundant to areas where they have been extirpated; and, compare the success of reintroduction of cultured versus wild fishes in establishing reproducing populations.

Impacts
What was accomplished under these goals? Goal 1) I described three new speices ofSalvelinusfrom the Great Smoky Mountains National Park, Tennessee. Additionally, a subspecies of cichlid from the Zambezi River, Africa was elevated to species level. Goal 2) I used the CT scanner to determine differences in the head morphology of theSalvelinusand the cichlid referenced above. Additionally, my students and I are preparing a paper on a technique that generates direct measurements from CT scans. Finally, I have used CT scans to determine morphological differences of two new species of anabantids from Angola, which I am describing with colleagues from the South African Institute of Aquatic Biodiversity in South Africa. Goal 3) I secured additional funding to continue the work on the endangered Chesapeake Logperch. I determined selected habitat, diet, age and growth of this species and a publication is currently being written. I continued the work on the introduced Round Goby in Pennsylvania and my students and I have completed the work on diet changes in native fishes when the Round Goby is present. In collaboration with the US Geological Survey (USGS) laboratory in West Virginia, we have determined which species of mussels are being consumed by the Round Goby in the Allegeheny River Drainage by analyzing environmental DNA (eDNA) of the stomachs of the Round Goby. My students have I have documented that Round Goby consume mussels using artificial streams and have found unionid mussels in the stomachs of Round Gobies that have been captured. Goal 4) We have cultured some 6000 Chesapeake Logperch in the facilites in the Forest Resources Building and at Rock Springs. We have tagged and introduced some 700 fishes into selected sites in the Susquehanna River near York, Pennsylvania. Additionally, we have captured over 150 wild Chesapeake Logperch, tagged them, and introduced them in the Susquehanna River near York, Pennsylvania. We are currently holding cultured fishes to maturity to determine if they are capable of breeding and to introduce and track them in 2021.

Publications

  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Mueller, S. J., C. Bradshaw-Wilson, and J. R. Stauffer, Jr. 2020. Dietary variability of the Rainbow Darter (Etheostoma caeruleum) in northwestern Pennsylvania. Northeastern Naturalist 27:26327
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Stauffer, J. R., Jr. 2020. Description of three species of Salvelinus (Teleostei: Salmonidae) from the Great Smoky Mountains National Park, Tennessee. Ichthyological Explorations of Freshwaters 30:97110.
  • Type: Journal Articles Status: Published Year Published: 2020 Citation: Stauffer, J. R., Jr., R. Bills, P. H. Skelton, and O. Weyl. 2020. Re-elevation to species level and redescription of Serranochromis jallae and Serranochromis robustus (Teleostei: Cichlidae). Zootaxa 4858:126134.