Progress 05/15/23 to 05/14/24
Outputs
Target Audience:Recruited graduate students trained in food safety research and industry needs. • Food safety stakeholders across U.S. • Meat processers in TX, NE, CA and other U.S. states. • Food process technology licensing partner(s). Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Recruited graduate students trained in food safety research and industry needs. How have the results been disseminated to communities of interest?Refereed research publications and presentation abstracts. Annual progress report and final report to USDA-AFRI What do you plan to do during the next reporting period to accomplish the goals?Evaluate the biophysical factors that govern biofilm detachment and dispersal, natively in the food processing facility.
Impacts
What was accomplished under these goals?
Improved understating on how foodborne pathogens (E. coli O157:H7 and Salmonella enterica) are protected and released into the food processing environment from biofilms Knowledge on effect of spatial location and spatial distribution of food pathogen in the mixed-species biofilms to their sanitizer tolerance. Quantitative knowledge on the dispersal of E. coli O157:H7 and Salmonella in multi-species biofilm located in the drain (hotspot) to the processing environment.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Chen Q, Wang R, Bosilevac JM, Guragain M, Chitlapilly Dass S. A novel method using a differential staining fluorescence microscopy (DSFM) to track the location of enteric pathogens within mixed-species biofilms. Sci Rep. 2023 Sep 16;13(1):15388. doi: 10.1038/s41598-023-42564-6. PMID: 37717059; PMCID: PMC10505192.
- Type:
Journal Articles
Status:
Published
Year Published:
2024
Citation:
Chen Q, Palanisamy V, Wang R, Bosilevac JM, Chitlapilly Dass S. Salmonella-induced microbiome profile in response to sanitation by quaternary ammonium chloride. Microbiol Spectr. 2024 Feb 6;12(2):e0234623. doi: 10.1128/spectrum.02346-23. Epub 2024 Jan 16. PMID: 38226804; PMCID: PMC10846233.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Aguayo G, Mathijssen AJTM, Ulloa HN, Soto R, Guzman-Lastra F. Floating active carpets drive transport and aggregation in aquatic ecosystems. arXiv:2312.11764 Published online December 18, 2023.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Palanisamy, V., Bosilevac, J. M., Barkhouse, D. A., Velez, S. E. & Chitlapilly Dass, S. Shotgun-metagenomics reveals a highly diverse and communal microbial network present in the drains of three beef-processing plants. Front Cell Infect Microbiol 13, doi:10.3389/fcimb.2023.1240138 (2023)
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Progress 05/15/22 to 05/14/23
Outputs
Target Audience:Recruited graduate students trained in food safety research and industry needs. • Food safety stakeholders across U.S. • Meat processers in TX, NE,CA and other U.S. states. • Food process technology licensing partner(s). Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Recruited graduate students trained in food safety research and industry needs. How have the results been disseminated to communities of interest?Refereed research publications and presentation abstracts. •Annual progress report and final report to USDA-AFRI. What do you plan to do during the next reporting period to accomplish the goals?Evaluate the biophysical factors that govern biofilm detachment and dispersal, natively in the food processing facility.
Impacts
What was accomplished under these goals?
•Improved understating on how foodborne pathogens (E. coli O157:H7 and Salmonella enterica) are protected and releasedinto the food processing environment from biofilms. • Knowledge on effect of spatial location and spatial distribution of food pathogen in the mixed-species biofilms to theirsanitizer tolerance. • Quantitative knowledge on the dispersal of E. coli O157:H7 and Salmonella in multi-species biofilm located in the drain(hotspot) to the processing environment.
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2023
Citation:
Mathijssen, A.J.T.M.; Lisicki, M.; Prakash, V.N.; Mossige, E.J.L. Culinary fluid mechanics and other currents in food science. Reviews of Modern Physics 2023, 95, 025004, doi:10.1103/RevModPhys.95.025004.
- Type:
Journal Articles
Status:
Accepted
Year Published:
2022
Citation:
Gerald G. Fuller, Maciej Lisicki, Arnold J. T. M. Mathijssen, Endre J. L. Mossige, Rossana Pasquino, Vivek N. Prakash, Laurence Ramos; Kitchen flows: Making science more accessible, affordable, and curiosity driven. Physics of Fluids 1 November 2022; 34 (11): 110401. https://doi.org/10.1063/5.0131565
- Type:
Journal Articles
Status:
Under Review
Year Published:
2023
Citation:
Palanisamy V, Bosilevac M. J., Barkhouse A. D., Velez E. S.,and Chitlapilly Dass, S * (2023) Shotgun-metagenomics reveals a highly diverse and communal microbial network present in the drains of three beef-processing plants. Frontiers in Cellular and Infection Microbiology
- Type:
Journal Articles
Status:
Under Review
Year Published:
2023
Citation:
Chen, Q, Palanisamy, V, Bosilevac, M.J., Wang, R and Chitlapilly Dass, S*. Salmonella-induced microbiome profile in response to sanitation by quaternary ammonium chloride. Spectrum Microbiology
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Progress 05/15/21 to 05/14/22
Outputs
Target Audience:Recruited graduate students trained in food safety research and industry needs. • Food safety stakeholders across U.S. • Meat processers in TX, NE,CA and other U.S. states. • Food process technology licensing partner(s). Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Recruited graduate students trained in food safety research and industry needs. How have the results been disseminated to communities of interest?Refereed research publications and presentation abstracts. •Annual progress report and final report to USDA-AFRI What do you plan to do during the next reporting period to accomplish the goals?Examine whether spatial organization and location of food-pathogen within the multispecies biofilm impacts sanitizer tolerance Evaluate the biophysical factors that govern biofilm detachment and dispersal, natively in the food processing facility
Impacts
What was accomplished under these goals?
Improved understating on how foodborne pathogens (E. coli O157:H7 and Salmonella enterica) are protected and released into the food processing environment from biofilms • Knowledge on effect of spatial location and spatial distribution of food pathogen in the mixed-species biofilms to their sanitizer tolerance. • Quantitative knowledge on the dispersal of E. coli O157:H7 and Salmonella in multi-species biofilm located in the drain (hotspot) to the processing environment.
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2022
Citation:
Wang, R, Bosilivac, B, Chitlapilly Dass, S. Characterization of Salmonella strains and environmental microorganisms isolated from a meat plant with Salmonella recurrence. Meat and Muscle Biology 2022 (Accepted)
- Type:
Journal Articles
Status:
Accepted
Year Published:
2021
Citation:
Chenyu Jin, Yibo Chen, Corinna C. Maass, and Arnold J.?T.?M. Mathijssen Entrainment and Confinement Amplify Transport by Schooling Microswimmers.
Phys. Rev. Lett. 127, 088006 Published 20 August 2021
- Type:
Journal Articles
Status:
Accepted
Year Published:
2021
Citation:
Demir�rs, A. F., Aykut, S., Ganzeboom, S., Meier, Y. A., Hardeman, R., de, J., Mathijssen, A. J. T. M., Poloni, E., Carpenter, J. A., �nl�, C., Zenh�usern, D., Amphibious Transport of Fluids and Solids by Soft Magnetic Carpets. Adv. Sci. 2021, 8, 2102510. https://doi.org/10.1002/advs.202102510
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Progress 05/15/20 to 05/14/21
Outputs
Target Audience: Recruited graduate students , undergraduate students and postdoctoral research assscoiate trained in food safety research and industry needs. Changes/Problems:We are in the process of moving our Subaward from Satnford University to Univeristy of Pennsilvania. Dr. Mau Prakas's Post doc at Standford University ( Dr. Arnold Mathjssen) was invloved in working on the objectives 2 and 3. Dr. arnold Mathjssen has now taken up Assistant professor position at University of pennsylvania. Dr. Manu praskas has kindly offered to move the subaward to Dr. Arnold Mathjssen with aggrement with us. Dr. Mathjssen has already published two papers and one paper under review from this project. What opportunities for training and professional development has the project provided?Training Activity: Ms. Qiyue Chen employed as a graduate student at Texas A&M on this project has a BS in Food Science from University of Nebraska. She was previously trained in classical microbiology. In her first year at Texas A&M, she has been trained in advanced high-resolution microscopy and bioinformatics to analysis complex OMICS data set. In addition, as this is a cross-disciplinary project, Ms. Chen is currently trained by Co-PIs (from USDA-ARS MARC and UPenn) on microfluidics and biofilm biology. Similar, the biophysics postdoc at UPenn Dr. Ercag Pince is being trained in OMICS and biofilm by the PI and Co-PI at (Texas A&M and USDA-ARS- USMARC). Professional Training: May 18- July 30 2021, we have summer training for all students, technician and scientists involved at Texas A&M. We will exchange results, work on developing new techniques and train on cross-disciplinary activities. How have the results been disseminated to communities of interest?The results from the project has been disseminated as research papers. This has resulted in 2 plublished work and 2 papers under review for publication. We are working with Texas AgriLife beef extension specialist to develop white paers based on our finsiginf to be distributed to beef processors in Texas and Nebraska. What do you plan to do during the next reporting period to accomplish the goals?We plan on working on the first three objectives to have susbtanial data to fit into a risk model (Objective 4). Goals: ompare and characterize diversity, stability and resilience of food pathogen and drain mixed-species biofilm in response to commonly used meat processing sanitizers Examine whether spatial organization and location of food-pathogen within the multispecies biofilm impacts sanitizer tolerance Evaluate the biophysical factors that govern biofilm detachment and dispersal, natively in the food processing facility
Impacts
What was accomplished under these goals?
Objective 1: Compare and characterize diversity, stability and resilience of food pathogen and drain mixed-species biofilm in response to commonly used meat processing sanitizers We analyzed the environmental microbial communities at a meat processing plant with self-reported E. Coli O157:H7 and Salmonella recurrence, and investigated the potential mechanisms by which the E. Coli O157:H7 and Salmonella strains could survive and persist in that local environment. To do so, floor drain samples from various locations at the plant were collected. Microbial communities in the drain samples and the E. Coli O157:H7 and Salmonella strains that were isolated from the same plant were phenotypically and genetically characterized for biofilm forming ability and tolerance against sanitization. The species composition of the mixed biofilm communities was further analyzed and related to the observed phenotypes of E. Coli O157:H7 and Salmonella recruitment into the community and their subsequent stress tolerance in an attempt to reveal the mechanism of how the interspecies interactions within the mixed community would affect environmental biofilm formation, pathogen colonization in the environment, and their ability to survive and persist. Objective 2. Examine whether spatial organization and location of food-pathogen within the multispecies biofilm impacts sanitizer tolerance Our work on flagellar activity has demonstrated that the spatial patterning of bacterial colonies within a biofilm can influence their nutrient and oxygen supply via self-generated flows. Swimming bacteria tend to accumulate on surfaces and self-assemble into 'active carpets' that generate coherent long-ranged flows. These flows arise from the coordinated action of many flagella that collectively can act like a pump. We developed a full theory to describe these flows, which we applied to bacterial swarming, topological pattern formation and clustering. Indeed, shortly afterwards experiments confirmed the emergence of long-ranged fluid transport in bacterial colonies. The measured flows circulate around the colony at a speed of ~30 m/s. Thus, these bacterial carpets can facilitate the transport of nutrients or oxygen, which feed back into activity and stronger flows. Moreover, the self-generated currents can also help with the spreading of the bacteria themselves. Therefore, pathogenic dispersal is likely enhanced by collectively generated flows of active carpets. Our findings also confirmed that bacterial self-organization influences population structure and material distribution in colonies. Objective 3: Evaluate the biophysical factors that govern biofilm detachment and dispersal, natively in the food processing facility Our work on the biofilm dispersal showed that, bacteria in the drainage pipes are frequently subjected to external rinsing flows. Counterintuitively, these rinsing flows may in fact enhance bacterial contamination because of the ability of bacteria to swim upstream, using a mechanism called 'rheotaxis'. Here cells can reorient with respect to flow gradients and therefore align against imposed currents. We investigated this reorientation mechanism using 3D tracking of bacteria in microfluidic channels, applying external shear flows and quantifying their ability to move upstream. From these experiments the mechanism responsible for bacterial reorientation against flows as a "weathervane effect" can be explained: The bacterial flagella are carried downstream, like a flag in the wind, so the cell body points upstream. We have also shown that rheotaxis could be used by micro-robots to transport cargo upstream. Moreover, upstream swimming may lead to "super-contamination" in narrow ducts because of the broad run-time distribution of their flagellar motors. To understand this flow-induced contamination we developed a theoretical analysis to explain the four different rheotaxis regimes for individual swimmers.
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2021
Citation:
Guzm�n-Lastra, F., L�wen, H. & Mathijssen, A.J.T.M. Active carpets drive non-equilibrium diffusion and enhanced molecular fluxes. Nat Commun 12, 1906 (2021).
- Type:
Journal Articles
Status:
Accepted
Year Published:
2020
Citation:
Tuning the Upstream Swimming of Microrobots by Shape and Cargo Size
Abdallah Daddi-Moussa-Ider, Maciej Lisicki, and Arnold J.T.M. Mathijssen
Phys. Rev. Applied 14, 024071 Published 25 August 2020
- Type:
Journal Articles
Status:
Under Review
Year Published:
2021
Citation:
Jin C, Chen Y, Maass CC, Mathijssen AJTM, Collective entrainment amplifies transport by schooling micro-swimmers , Under Review at Phys. Rev. Lett. (2021)
- Type:
Journal Articles
Status:
Under Review
Year Published:
2021
Citation:
Chitlapilly Dass, Sapna, Wang, Rong and Bosilivac, Mick. Impact of mixed biofilm formation with environmental microorganisms on Salmonella Spp. survival against sanitization, Under Review Appiled and Environmental Microbiology (2021)
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