Progress 05/15/23 to 05/14/24
Outputs
Target Audience:The knowledge gained through this project was shared with extension agents, the Florida grape growers association, muscadine products companies, grape breeders, biochemists, and geneticists who are interested in enhancing the national grape industry and commercialization. Further, we have generated a training program that targets students in the local high schools and FAMU scientists and students to provide training in the areas of viticulture research (i.e., biochemistry, biology, computational biology, and others). The current trend in food consumption demands liability in developing innovative foods with superior nutritional qualities to increase consumer acceptance and market value for sustaining production. Muscadine grapes are known to possess enhanced health-promoting molecules due to the synthesis of unique phytochemicals context with particular health-benefit properties that distinguish them from other grape species. Genetic variation among muscadine genotypes can influence their phytochemicals content and composition, and thus their health value. The absence of definite molecules and molecular mechanisms underlying their synthesis and accumulation in berries are the major crucial factors that affect the nutraceutical qualities of muscadine grapes. The objectives, technologies, and research outcomes have been discussed in several meetings with growers and grape breeders during the fourth-year grant: Grape Field Day (UF/IFAS), Citra, FL, May 18, 2023. The 2023 Florida State Horticultural Society (FSHS) Annual Conference, Daytona Beach, FL, June 10-13, 2023. North American Grape Breeders & Vitis-Muscadinia Alliance Conference (UA), Fayetteville, AR, October 3-5, 2023. Florida Wine and Grape Growers Association (FWGGA) 2023 Annual Conference, Deland, FL, January 11-12, 2024. Southeast Regional Fruit & Vegetable Conference 2024, Savannah, GA, January 12-13, 2024. Legislature visit to the Center for Viticulture, CAFS, FAMU that includes city/state representatives and industry representatives, Tallahassee, FL, January 23, 2024. Association of 1890 Research Directors (ARD) Research Symposium 2024, Nashville, TN, April 6-9, 2024 (two presentations). Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?We constantly strive to enhance the learning experience of our students in agreement with FAMU's motto of "Excellence with Caring" by improving the learning procedure and qualities. In 2021, we officially started a "Summer Biotech Academy" that targets a diverse pool of minority high school students and undergraduate FAMU students. The "Summer Biotech Academy" activities are sustained. The main goal of the program is to inspire students' awareness by exposing them to real-life examples and objectives, which develop communication, leadership, and management skills, encourage students to pursue advanced graduate degrees, expand students' intellectual knowledge and lab experiences and prepare them to better cope for a future workforce to empower and sustain the industry. During the life of the project, we have trained thirty-eight students (15 high school students and 23 FAMU undergraduate students). How have the results been disseminated to communities of interest?The knowledge gained through this project was shared with extension agents, the Florida grape growers' association, muscadine products companies, grape breeders, biochemists, and geneticists who are interested in enhancing the national grape industry and commercialization. In addition to the previous year's publications, three new peer-reviewed manuscripts have been produced. Further, we have generated a training program that targets students in the local high schools and FAMU scientists and students to provide training in the areas of viticulture research (i.e., biochemistry, biology, computational biology, and others). The objectives, technologies, and research outcomes of this project have been discussed in several meetings with growers and grape breeders during the previous year grant (7 Presentations). What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
We have generated and published three manuscripts. The designated pipeline for anticancer activity in muscadine grapes has been tested with six different cancer cell lines, including the African American Breast Cancer (MDA-MB-468), Prostate Cancer (C42B), African American Prostate Cancer (MDA PCa 2b), African American Liver Cancer (HEP3B), Skin Cancer (A431), and Pancreatic Cancer (PANC) to ensure the results' creditability. The separated berry tissues (skin and seeds) of the 360 muscadine genotypes were used for this study. The cytotoxicity data resulting from the muscadine population using the two berry tissues was comprehensively analyzed, and the cross-correlation between cell cytotoxicity and different factors of total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), and antioxidant activities (DPPH and FRAP) was performed. To track and identify muscadine metabolites underling cancer cytotoxicity, the muscadine genotypes exhibited high and low cytotoxicity (for each cancer cell line tested) based on skin or seed tissues were identified. The skin/seed tissues were collected at four developmental stages, designated as fruit-set (FS), green (G), véraison (V), and ripe (R). All developmental stages were subjected to methanol extraction and their cytotoxicity was determined with respective cancer cell line. The experiment was conducted in triplicate using four different extract quantities (125, 250, 500, and 1000 ng/ml) and three different incubation times (24, 48, and 72 hours). After recognition of the cytotoxicity pattern among genotypes and developmental stages, the methanolic extraction was subjected to Plant Untargeted Metabolome Profiling to identify metabolites underlying anticancer activity. However, we performed this experiment only with the African American Breast Cancer (MDA-MB-468). Conducting Genome-Wide Association Studies (GWAS) analysis allowed us to identify two major QTLs associated with the bioactivity of skin or seed tissues. Analyzing the function of genes within the identified QTLs suggested an unexpected mechanism beyond our expectations. It proposed that cancer cell cytotoxicity is induced by a metabolic mechanism via inhibiting breast cancer cells' immunity and through influencing cells' growth and development. Further, we conducted transcriptome profiling of respective muscadine genotypes to identify the differentially expressed genes (DEGs) within the QTLs. The association between metabolomic, genomic, and transcriptomic data will be used to identify and confirm the inhibitory mechanism.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Scheiner J, El-Sharkawy I, Humphries AF, Ren Z, Pontasch F, Labay A, Kamas J, Tsolova V. (2023). Blanc Du Soleil: A wine grape for the southeast United States. HortScience 58: 610 613.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Darwish AG, El-Sharkawy I, Tang C, Rao Q, Tan J. (2023) Investigation of antioxidant and cytotoxicity activities of chocolate fortified with muscadine grape pomace. Foods 12: 3315.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
Ismail A, Pervaiz T, Comstock S, Bodaghi S, Rezk A, Vidalakis G, El-Sharkawy I, Obenland D, El-kereamy A. (2023) Unraveling the occasional occurrence of berry astringency in table grape cv. Scarlet Royal: A physiological and transcriptomic analysis. Frontiers in Plant Science 14: 1271251.
- Type:
Journal Articles
Status:
Other
Year Published:
2024
Citation:
Darwish AG, Das PR, Ali NA, Walters KJ, Soliman KFA, El-Sharkawy I. (2024) Diversity in muscadine genotypes enables discrepancy in the bioactive compounds underling African American breast cancer cytotoxicity. In Progress
|
Progress 05/15/20 to 05/14/24
Outputs
Target Audience:The knowledge gained through this project was shared with extension agents, the Florida grape growers association, muscadine products companies, grape breeders, biochemists, and geneticists who are interested in enhancing the national grape industry and commercialization. Further, we have generated a training program that targets students in the local high schools and FAMU scientists and students to provide training in the areas of viticulture research (i.e., biochemistry, biology, computational biology, and others). The current trend in food consumption demands liability in developing innovative foods with superior nutritional qualities to increase consumer acceptance and market value for sustaining production. Muscadine grapes are known to possess enhanced health-promoting molecules due to the synthesis of unique phytochemicals context with particular health-benefit properties that distinguish them from other grape species. Genetic variation among muscadine genotypes can influence their phytochemicals content and composition, and thus their health value. The absence of definite molecules and molecular mechanisms underlying their synthesis and accumulation in berries are the major crucial factors that affect the nutraceutical qualities of muscadine grapes. The objectives, technologies, and research outcomes have been presented in several meetings during the project's life with growers and grape breeders (20 meetings): Grape Field Day at UF/IFAS (3 Presentations). The Florida State Horticultural Society (FSHS) Annual Conference (3 Presentations). North American Grape Breeders (2 Presentations). The Virtual Conference of the North Carolina Grape Growers Association (2 Presentations). Florida Wine and Grape Growers Association (FWGGA) Annual Conference (3 Presentations). Southeast Regional Fruit & Vegetable Conference (1 Presentation). Legislature visit to the Center for Viticulture, CAFS, FAMU that includes city/state representatives and industry representatives (2 Presentations). Association of 1890 Research Directors (ARD) Research Symposium (4 Presentations). Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?We constantly strive to enhance the learning experience of our students and trainees in agreement with FAMU's motto of "Excellence with Caring" by improving the learning procedure and qualities. In 2021, we officially started a "Summer Biotech Academy" that targets a diverse pool of minority high school students and undergraduate FAMU students. The "Summer Biotech Academy" activities are sustained. During the life of the project, we have trained thirty-eight students (15 high school students and 23 FAMU undergraduate students). Further, during the project life 3 faculty/non-students were engaged in that project and we recruited and trained 25 students and scientists, including 5 members in the field of Plant Science (one undergraduate students and 4 graduates), 10 members in the field of Biochemistry and Molecular Biology (one undergraduate students, 4 graduates, and 6 post-doctorates), 4 post-doctorates in Genetics, Biomathematics, 4 post-doctorates in Bioinformatics and Computational Biology, and two graduates as Biology Technician. Our main goal is to inspire students'/trainees' awareness by exposing them to real-life examples and objectives, which develop communication, leadership, and management skills, encourage students to pursue advanced graduate degrees, expand students' intellectual knowledge and lab experiences, and prepare them to better cope for a future workforce to empower and sustain the industry. How have the results been disseminated to communities of interest?The knowledge gained through this project was shared with extension agents, the Florida grape growers association, muscadine products companies, grape breeders, and geneticists who are interested in enhancing the national grape industry and commercialization. During the life of the project, we have produced 16 peer-reviewed publications and one extra major publication is in progress. Further, we have established two major database browsers, Sothern Grapes Genome/Transcriptome data and Sothern Grapes & Specialty Crops Breedomics that has been designed for long-term storage of all the genomic, transcriptomic, phenotypic, and metabolic data. Moreover, we have generated a training program (3 modules) that targets students in the local high schools and FAMU scientists and students to provide training in the areas of viticulture/plant research (i.e., biology, computational biology, biochemistry, and others). Finally, we have been dynamically involved in enhancing the CAFS/FAMU mission of student recruitment via generating a website and a video highlighting the excellent FAMU capacities for the quality of education and research with outstanding examples of students who graduated under the CAFS programs. The current trend in food consumption demands liability in developing innovative foods with superior nutritional qualities to increase consumer acceptance and market value for sustaining production. Muscadine grapes are known to possess enhanced health-promoting molecules due to the synthesis of unique phytochemicals context with particular health-benefit properties that distinguish them from other grape species. Genetic variation among muscadine genotypes can influence their phytochemicals content and composition, and thus their health value. The absence of definite molecules and molecular mechanisms underlying their synthesis and accumulation in berries are the major crucial factors that affect the nutraceutical qualities of muscadine grapes. The objectives, technologies, and research outcomes have been discussed in several meetings with growers and grape breeders during the life of the project (20 presentations in total). What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
We have generated and published three manuscripts. The designated pipeline for anticancer activity in muscadine grapes has been tested with six different cancer cell lines, including the African American Breast Cancer (MDA-MB-468), Prostate Cancer (C42B), African American Prostate Cancer (MDA PCa 2b), African American Liver Cancer (HEP3B), Skin Cancer (A431), and Pancreatic Cancer (PANC) to ensure the results' creditability. The separated berry tissues (skin and seeds) of the 360 muscadine genotypes were used for this study. The cytotoxicity data resulting from the muscadine population using the two berry tissues was comprehensively analyzed, and the cross-correlation between cell cytotoxicity and different factors of total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), and antioxidant activities (DPPH and FRAP) was performed. To track and identify muscadine metabolites underling cancer cytotoxicity, the muscadine genotypes exhibited high/low cytotoxicity (for each cancer cell line tested) based on skin or seed tissues were identified. The skin/seed tissues of were collected at four developmental stages, designated as fruit-set (FS), green (G), veraison (V), and ripe (R). All developmental stages were subjected to methanol extraction and their cytotoxicity was determined with respective cancer cell line. After recognition of the cytotoxicity pattern among developmental stages, the methanolic extraction was subjected to Plant Untargeted Metabolome Profiling to identify metabolites underlying anticancer activity. However, we performed this experiment only with the African American Breast Cancer (MDA-MB-468), Prostate Cancer (C42B), and African American Prostate Cancer (MDA PCa 2b) due to the lack of fund because we were not originally planning to work with all these cancer cell lines. Conducting Genome-Wide Association Studies (GWAS) analysis allowed us to identify two major QTLs associated with the bioactivity of skin or seed tissues. Analyzing the function of genes within the identified QTLs suggested an unexpected mechanism beyond our expectations. It proposed that cancer cell cytotoxicity is induced by a molecular mechanism via inhibiting cells' immunity and a metabolic mechanism by influencing cells' growth and development. Further, we conducted transcriptome profiling of respective muscadine genotypes. The association between metabolomic, genomic, and transcriptomc data will be used to identify the inhibitory mechanism, which differentiate between the type of cancer cell line (i.e., Breast and Prostate Cancer cell lines) and between races for the same cancer type (African American and Caucasian Prostate Cancer cell lines).
Publications
|
Progress 05/15/22 to 05/14/23
Outputs
Target Audience:The knowledge gained through this project was shared with extension agents, the Florida grape grower's association, muscadine products companies, grape breeders, biochemists, and geneticists who are interested in enhancing the national grape industry and commercialization. Further, we have generated a training program that targets students in the local high schools and FAMU scientists and students to provide training in the areas of viticulture research (i.e., biochemistry, biology, computational biology, and others). The current trend in food consumption demands liability in developing innovative foods with superior nutritional qualities to increase consumer acceptance and market value for sustaining production. Muscadine grapes are known to possess enhanced health-promoting molecules due to the synthesis of unique phytochemicals context with particular health-benefit properties that distinguish them from other grape species. Genetic variation among muscadine genotypes can influence their phytochemicals content and composition, and thus their health value. The absence of definite molecules and molecular mechanisms underlying their synthesis and accumulation in berries are the major crucial factors that affect the nutraceutical qualities of muscadine grapes. The objectives and technologies used in this proposal have been already discussed in several meetings with growers and grape breeders during the third-year grant: - The 20th Biannual Research Symposium - Association of 1890 Research Directors (ARD), Atlanta, GA, April 2 - 5, 2022 (two presentations). - The 2022 Florida State Horticultural Society (FSHS) Annual Conference, Sarasota, FL, June 5 - 7, 2022. - Florida Wine and Grape Growers Association (FWGGA) 2022 Annual Conference, Deland, FL, June 17 - 18, 2022. - Legislature visit to the Center for Viticulture, CAFS, FAMU that includes city/state representatives, industry representatives, and policymakers, Tallahassee, FL, February 8, 2023. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?We constantly strive to enhance the learning experience of our students in agreement with FAMU's motto of "Excellence with Caring" by improving the learning procedure and qualities. In 2021, we officially started a "Summer Biotech Academy" that targets a diverse pool of minority high school students and undergraduate FAMU students. The "Summer Biotech Academy" activities are sustained. The main goal of the program is to inspire students' awareness by exposing them to real-life examples and objectives, which develop communication, leadership, and management skills, encourage students to pursue advanced graduate degrees, expand students' intellectual knowledge and lab experiences and prepare them to better cope for a future workforce to empower and sustain the industry. Thus far, we have trained twenty-six students (10 high school students and 16 FAMU undergraduate students). How have the results been disseminated to communities of interest?The knowledge gained through this project was shared with extension agents, the Florida grape growers' association, muscadine products companies, grape breeders, biochemists, and geneticists who are interested in enhancing the national grape industry and commercialization. In addition to the previous year's publications, three new peer-reviewed manuscripts have been produced. Further, we have generated a training program that targets students in the local high schools and FAMU scientists and students to provide training in the areas of viticulture research (i.e., biochemistry, biology, computational biology, and others). The current trend in food consumption demands liability in developing innovative foods with superior nutritional qualities to increase consumer acceptance and market value for sustaining production. Muscadine grapes are known to possess enhanced health-promoting molecules due to the synthesis of unique phytochemicals context with particular health-benefit properties that distinguish them from other grape species. Genetic variation among muscadine genotypes can influence their phytochemicals content and composition, and thus their health value. The absence of definite molecules and molecular mechanisms underlying their synthesis and accumulation in berries are the major crucial factors that affect the nutraceutical qualities of muscadine grapes. The objectives and technologies used in this proposal have already been discussed in several meetings with growers and grape breeders during the first year grant: - The 20th Biannual Research Symposium - Association of 1890 Research Directors (ARD), Atlanta, GA, April 2 - 5, 2022 (two presentations). - The 2022 Florida State Horticultural Society (FSHS) Annual Conference, Sarasota, FL, June 5 - 7, 2022. - Florida Wine and Grape Growers Association (FWGGA) 2022 Annual Conference, Deland, FL, June 17 - 18, 2022. - Legislature visiting even to the Center for Viticulture, CAFS, FAMU that includes city, state representatives and agencies, industry representatives, and policymakers, Tallahassee, FL, February 8, 2023. What do you plan to do during the next reporting period to accomplish the goals?Based on the obtained results, our activities during the next year will include the following: 1- Identify and test potential muscadine berry metabolomes contributing to African American Breast Cancer cytotoxicity. 2- Establish the potential molecular mechanism affecting cancer cells' immunity. In the meantime, we will continue our Summer Biotech Academy activities and our efforts to disseminate the research outcomes and assess the socioeconomic effects of producing such next-generation grape cultivars with multi-beneficial targets.
Impacts
What was accomplished under these goals?
1- We have publishedthe remaining antioxidant-related work under objectives 1 and 2. Three manuscripts have been generated and published. 2- The designatedpipeline for anticancer activity in muscadine grapes has been tested with six different cancer cell lines, including the African American Breast Cancer (MDA-MB-468), Prostate Cancer (C42B), African American Prostate Cancer (MDA PCa 2b), African American Liver Cancer (HEP3B), Skin Cancer (A431), and Pancreatic Cancer (PANC)to ensure the results' creditability. The separated berry tissues (skin and seeds) of the 360muscadinegenotypes were used for this study. 3- The cytotoxicity data resulting from the muscadine population using the two berry tissues with African American Breast Cancer (MDA-MB-468)was comprehensively analyzed, and the cross-correlation between cell cytotoxicity and different factors of total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), and antioxidant activities (DPPH and FRAP) was performed. 4- ConductingGenome-Wide Association Studies (GWAS) analysis allowed us to identify two major QTLs associated with the bioactivity of skin and seed tissues. Analyzing the function of genes within the identified QTLs suggested an unexpected mechanism beyond our expectations. It proposed that cancer cell cytotoxicity is induced by a molecular mechanism via inhibiting cells' immunity and a metabolic mechanism by influencing cells' growth and development.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
" Park M, Darwish AG, Elhag RI, Tsolova V, Soliman KFA, El-Sharkawy I. (2022) A multi-locus genome-wide association study reveals the genetics underlying muscadine antioxidant in berry skin. Frontiers in Plant Science 13: 969301.
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
" Ismail A, Gajjar P, Park M, Mahboob M, Tsolova V, Jayasankar S, Darwish AG, El-Sharkawy I. (2022) A recessive mutation in muscadine grapes causes berry color-loss without influencing anthocyanin pathway. Communications Biology 5: 1012.
- Type:
Journal Articles
Status:
Published
Year Published:
2023
Citation:
" Darwish AG, Moniruzzaman M, Tsolova V, El-Sharkawy I. (2023) Integrating metabolomics and gene expression underlying potential biomarkers compounds associated with antioxidant activity in southern grape seeds. Metabolites 13: 210.
|
Progress 05/15/21 to 05/14/22
Outputs
Target Audience:The knowledge gained through this project was shared with extension agents, Florida grape grower's association, muscadine products companies, grape breeders, biochemists, and geneticists who are interested in enhancing national grape industry and commercialization. Further, we have generated a training program that targets students and teachers in the local high schools, and FAMU scientists and students to provide training in the areas of viticulture research (i.e. biochemistry, biology, computational biology, and others). Current trend in food consumption demands liability in the development of innovative foods with superior nutritional qualities to increase consumer acceptance and market value for sustaining production. Muscadine grapes are known to possess enhanced health-promoting molecules due to the synthesis of unique phytochemicals context with particular health benefit properties that distinct them from other grape species. Genetic variation among muscadine genotypes can influence their phytochemicals content and composition, and thus their health value. The absence of definite molecules and molecular mechanisms underlying their synthesis and accumulation in berries are the major crucial factors that affect the nutraceutical qualities of muscadine grapes. The objectives and technologies used in this proposal have been already discussed in several meetings with growers and grape breeders during the first year grant: - Florida Wine and Grape Growers Association (FWGGA) 2021 Annual Conference, Deland, FL. - The UF 2021 Virtual Grape Field Day, UF/IFAS Plant Science Research and Education Unit, Citra, FL. - The Virtual Conference of the North Carolina Grape Growers Association. - The Virtual Annual Meeting of the Florida State Horticultural Society. Changes/Problems:No problems during the reporting period. What opportunities for training and professional development has the project provided?We constantly strive to enhance the learning experience of our students in agreement with the FAMU's motto of "Excellence with Caring" by improving the learning procedure and qualities. Therefore, I have participated in multiple teaching enhancement programs at FAMU and became a Certified Online Instructor. In 2021, we have officially started a "Summer Biotech Academy" that targets a diverse pool of minority high school students and undergraduate FAMU students. The main goal of the program is to inspire students' awareness by exposing them to real-life examples and objectives, which develop communication, leadership, and management skills, encourage students to pursue advanced graduate degrees, expand students' intellectual knowledge and lab experiences, and prepare them to better cope for a future workforce to empower and sustain the industry. Thus far, we have trained nine students (8 high school students and 11 FAMU undergraduate students). How have the results been disseminated to communities of interest?The objectives and research outcomes achived, thus far, have been already discussed in several meetings with growers, grape products companies, and peer grape breeders during the second year of grant: The knowledge gained through this project was shared with extension agents, Florida grape grower's association, muscadine products companies, grape breeders, biochemists, and geneticists who are interested in enhancing national grape industry and commercialization. Further, we have generated a training program that targets students and teachers in the local high schools, and FAMU scientists and students to provide training in the areas of viticulture research (i.e. biochemistry, biology, computational biology, and others). Current trend in food consumption demands liability in the development of innovative foods with superior nutritional qualities to increase consumer acceptance and market value for sustaining production. Muscadine grapes are known to possess enhanced health-promoting molecules due to the synthesis of unique phytochemicals context with particular health benefit properties that distinct them from other grape species. Genetic variation among muscadine genotypes can influence their phytochemicals content and composition, and thus their health value. The absence of definite molecules and molecular mechanisms underlying their synthesis and accumulation in berries are the major crucial factors that affect the nutraceutical qualities of muscadine grapes. The objectives and technologies used in this proposal have been already discussed in several meetings with growers and grape breeders during the first year grant: - Florida Wine and Grape Growers Association (FWGGA) 2021 Annual Conference, Deland, FL. - The UF 2021 Virtual Grape Field Day, UF/IFAS Plant Science Research and Education Unit, Citra, FL. - The Virtual Conference of the North Carolina Grape Growers Association. - The Virtual Annual Meeting of the Florida State Horticultural Society. What do you plan to do during the next reporting period to accomplish the goals?In relation to anticancer activity in muscadine grape, we have finished the determination of biochemical properties and anticancer bioactive assays. We were able to identify the appropriate genotypes among population for detailed analysis. It remains for us to the LC-MS non-target metabolome profiling, and anticancer assay for purified compounds. In the meantime, we will continue our efforts to disseminate our research outcomes and assess the socioeconomic effects of producing such next generation grape cultivars with multi-beneficial targets.
Impacts
What was accomplished under these goals?
1- All the antioxidant related work under objectives 1 and 2 have been performed. Some of the work have been already published and others have been submitted for publication. 2- We have designed and esblished a pipeline for anticancer activity in muscadine grapes. The pipeline has been published and we are currently working in anticancer activity of muscadine grapes. 3- The objectives 3 and 4 conducted by applicable collaborators at the FAMU and the IFAS/UF are in progress.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Darwish AG, Das PR, Ismail A, Gajjar P, Balasubramani SP, Sheikh MB, Tsolova V, Sherif SM, El-Sharkawy I. (2021) Untargeted metabolomics and antioxidant capacities of muscadine grape genotypes during berry development. Antioxidants 10: 914.
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Das PR, Darwish AG, Ismail A, Haikal AM, Gajjar P, Balasubramani SP, Sheikh MB, Tsolova V, Soliman KFA, Sherif SM, El-Sharkawy I. (2022) Diversity in blueberry genotypes and developmental stages enables discrepancy in the bioactive compounds, metabolites, and cytotoxicity. Food Chemistry 374: 131632.
- Type:
Journal Articles
Status:
Published
Year Published:
2022
Citation:
Ismail A, Darwish AG, Park M, Gajjar P, Tsolova V, Soliman KFA, El-Sharkawy I. (2022) Transcriptome profiling during muscadine berry development reveals the dynamic of polyphenols metabolism. Frontiers in Plant Science 12: 818071.
- Type:
Journal Articles
Status:
Submitted
Year Published:
2022
Citation:
Ismail A, Darwish AG, Park M, Mahboob M, Gajjar P, Tsolova V, Jayasankar S., El-Sharkawy I. (2022) A recessive mutation in muscadine grapes causes berry color-loss without influencing anthocyanin pathway. Plant Physiology.
- Type:
Journal Articles
Status:
Submitted
Year Published:
2022
Citation:
Darwish AG, Park M, Tsolova V, El-Sharkawy I. (2022) Metabolome and transcriptome components underlying high antioxidant activity in muscadine seeds. Nutrients.
- Type:
Other
Status:
Published
Year Published:
2021
Citation:
Emily Laurence. (2021) This specific grape variety is linked to longevityand youll find it at your local grocery store. Interview in Well+Good magazine.
- Type:
Journal Articles
Status:
Submitted
Year Published:
2022
Citation:
Darwish AG, Park M, Tsolova V, El-Sharkawy I. (2022) A multilocus genome-wide association study reveals genetics underlying muscadine antioxidant in berry skin. International Journal of Molecular Sciences.
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2021
Citation:
Jiovan Campbell. (2021) Characterization of large muscadine (Muscadinia rotundifolia Michx.) population for diverse phenological and reproductive performance traits.
- Type:
Other
Status:
Published
Year Published:
2021
Citation:
Alexandria Brooks. (2021) Eating this variety of grapes could help you live a longer, healthier life. Interview in Woman's World magazine.
- Type:
Journal Articles
Status:
Published
Year Published:
2021
Citation:
Campbell J, Sarkhosh A, Habibi F, Gajjar P, Ismail A, Tsolova V, El-Sharkawy I. (2021) Evaluation of biochemical juice attributes and color-related traits in muscadine grape population. Foods 10: 1101.
|
Progress 05/15/20 to 05/14/21
Outputs
Target Audience:The knowledge gained through this project will be shared with extension agents, Florida grape grower's association, muscadine products companies, grape breeders, biochemists, and geneticists who are interested in enhancing national grape industry and commercialization. Other target audiences will be city, county, state representatives and agencies, policymakers, as well as the general public. Further, we have generated a training program that targets students and teachers in the local high schools, and FAMU students to provide training in the areas of viticulture research (i.e. physiology, biochemistry, biology, genomics, computational biology, and others). Current trend in food consumption demands liability in the development of innovative foods with superior nutritional qualities to increase consumer acceptance and market value for sustaining production. Muscadine grapes are known to possess enhanced health-promoting molecules due to the synthesis of unique phytochemicals context with particular health benefit properties that distinct them from other grape species. Genetic variation among muscadine genotypes can influence their phytochemicals content and composition, and thus their health value.The absence of definite molecules and molecular mechanisms underlying their synthesis and accumulation in berries are the major crucial factors that affect the nutraceutical qualities of muscadine grapes. The objectives and technologies used in this proposal have been already discussed in several meetings with growers and grape breeders during the first year grant: - Florida Wine and Grape Growers Association (FWGGA) 2020 Annual Conference, Deland, FL. - The North American Grape Breeders Conference, College of Agriculture, Mountain Grove, MO. - The University of Florida Muscadine Grape Day, UF/IFAS Plant Science Research and Education Unit, Citra, FL. - The Virtual Conference of the North Carolina Grape Growers Association. Changes/Problems:As it is already expected, there are several problem occurred during this period due to the spread of COVID-19 pandemic. This considerably affected the progression of our work at different levels. These problems started by early February and remain up to current day. Despite that, the team members worked during the year; trying to recover the season otherwise, we have to wait a full year to perform the experiments. In the middle of the first year, we have lost the efforts of some team members due to COVID infection or due to the close of the research site. This cause significant delay; however, we successfully performed the experiment and collected all the necessary materials. The work related to grape collection and wine making was not performed due to the lack personnel. In addition, we consumed significant amount of our time for the task related to meeting with growers and consumers. We were obligated to change our strategies of interaction from face to face into virtual. This transition is still not in perfect shape; there are troubleshooting that we face. Generally, we successfully covered considerable objectives out of the total project goals. To overcome the problem related to interaction with high school students and teachers, we decided to generate a descriptive video about the project objective that will be available on applicable websites at the FAMU and UF. This will be available by fall 2021. What opportunities for training and professional development has the project provided?Two MS-graduate students, a postdoctoral fellow, and four professionals were trained in samples collection and preparation, samples processing for extraction, determine active compounds, define biological activities and cytotoxicity, LC-MS data analysis, and molecular analysis. They are very well trained in bioinformatic, biochemistry, transcriptome, and genomic tools for sequence comparison among grape genotypes. How have the results been disseminated to communities of interest?The objectives and technologies used in this proposal have been already discussed in several meetings with growers, grape products companies, and peer grape breeders during the first year of grant: - Florida Wine and Grape Growers Association (FWGGA) 2020 Annual Conference, Deland, FL. - The North American Grape Breeders Conference, College of Agriculture, Mountain Grove, MO. - The University of Florida Muscadine Grape Day, UF/IFAS Plant Science Research and Education Unit, Citra, FL. - The Virtual Conference of the North Carolina Grape Growers Association. What do you plan to do during the next reporting period to accomplish the goals?In relation to antioxidant activity in muscadine grape, we have finished the determination of biochemical properties, antioxidant bioactive assays, the LC-MS non-target metabolome profiling, and the transcriptome analysis. It remains for us to perform the GWAS analysis among population. A manuscript related to this title is in progress. In relation to coloration and anthocyanin accumulation in muscadine berries, we have finished the determination of anthocyanin properties, determination of different color-related traits, define anthocyanin composition in muscadine berries using HPLC, GWAS analysis among population, and generating RNA-seq data. It remains for us to analyze the transcriptome data. A manuscript related to this title is in progress. From the previous, we can figure out that we have established a sloid pipeline that has integrated metabolomic, genomic, and transcriptomic strategies to reach our research objectives. This pipeline will be subjected to identify molecules associated with high breast anticancer activities and those that can distinguish between the two types of breast-cancer cell lines (African American and Caucasian). In the meantime, we will continue our efforts to disseminate our research outcomes and assess the socioeconomic effects of producing such next generation grape cultivars with multi-beneficial targets.
Impacts
What was accomplished under these goals?
Under objective 2 and through screening 340 muscadine genotypes for their total phenolic content (TPC), total flavonoid content (TFC), and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging antioxidant activity, we were able to identify 3 muscadine genotypes C5-9-1, Late Fry, and C6-10-2 exhibiting enhanced, intermediate, and low biochemical properties/DPPH activities, respectively. Samples from these three genotypes were collected at 6 different developmental stages, fruit-set (FS), pre-Veraison (Pre-V), Veraison (V), post-Veraison (Post-V), ripening (R), and seeds (S). These stages were subjected to several analysis, as follow, 1- Assessment for the changes in biochemical properties (TPC and TFC). 2- Determination of antioxidant activities using several types of assays, including DPPH, FRAP (Ferric Reducing Antioxidant Power), ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)], NBT (nitroblue tetrazolium), and CUPRAC (CUPric Reducing Antioxidant Capacity). 3- Non-target metabolome profiling. 4- Based on population analysis (340 individual genotypes) for the traits of TPC, TFC, and DPPH, we performed Genome Wide Association Studies (GWAS) analysis to determine critical QTLs associated with the enhanced biochemical properties and DPPH-based antioxidant activity. 5- Using the extremely two genotypes of C5-9-1 and C6-10-2, we have performed transcriptome analysis in order to determine the gene network associated with the enhanced biochemical properties and antioxidant activities. Further, the berries of the three selected genotypes exhibit dark-red color for C5-9-1 and C6-10-2 lines and bronze color for the standard cultivar Late Fry. Accordingly, we have performed several sets of experiments to determine the contribution of anthocyanin. This includes: 1- Determination of color properties of the population using different strategies, including total anthocyanin content (TAC), luminosity (L), hue degree (H°), and chroma (C). 2- Assessment of the anthocyanin composition during the 6 different developmental stages of the three genotypes using HPLC. 3- Assessment of the anthocyanin composition in the ripe berries of 9 muscadine genotypes, exhibiting different visual color (black, dark-red, red, bronze, and green) using HPLC. 4- Based on population analysis (340 individual genotypes) for the traits of TAC, L, H°, and C, we performed GWAS analysis to determine critical QTLs associated with anthocyanin accumulation. This allowed us to identify a particular QTL in Chr-4 that can distinguish between colored and non-colored berries. Screening the genes within the identified region in Chr-4 enabled us to identify a key gene in the anthocyanin biosynthesis pathway encodes for Glutathione S-transferase (GST) that is involved in the transportation of synthesized anthocyanin from cytoplasm to vacuole. Analysis of GST mRNA nucleotide sequence in several colored and non-colored muscadine genotypes highlighted one nucleotide substitution (C512 to T), causing predicted substitution in the amino acid residues Pro171 to Leu. 5- Using the extremely two genotypes of C5-9-1 and Late Fry, we have performed transcriptome analysis in order to determine the gene network associated with anthocyanin accumulation in muscadine berries.
Publications
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