Progress 12/11/19 to 09/30/20
Outputs Target Audience:The primary target audience includes cotton growers, seed company breeders and representatives, and private consultants working with the cotton industry in California and other cotton producing states, Some of the information developed on varietal performance in CA conditions will be primarily of interest to growers in the region. Other information on Pima cotton production and disease management and breeding for Fusarium race 4 resistance may be of broader interest across multiple cotton-growing states, since those research issues have somewhat limited coverage in other areas. Key cooperators who will interact on these projects include University of CA Farm Advisors, California State University Fresno staff, USDA-ARS cooperators and researchers in other cotton growing locations in the U.S. Primary interest in the general variety trial work is primarily from seed company representatives and breeders, plus growers in California. In terms of the Fusarium race 4 screening work, interest is more broad-based with interest from seed company breeders, two international cotton breeders, researchers from multiple U.S. cotton-producing states, plus representatives contacting us from locations including Australia, Greece, and Uzbekistan. Particularly in the case of the work on the fungal disease, race 4 Fusarium, some other target groups include interested parties in other U.S. states plus cotton industry organizations such as Cotton Incorporated and the National Cotton Council. The information produced relative to Fusarium disease issues with race 4 has been used by Farm Advisors in CA and Arizona for multiple years. Starting in 2017 and continuing currently, the project has responded to inquiries from groups located in Texas, New Mexico, Louisiana, Georgia, and Mexico, including both Cooperative Extension and other land grant and non land-grant universities as well as seed company representatives. Their interest has been in dissemination of University information to industry. This information is provided as web-based information for potential distribution to other cotton production areas in the United States, and to the Cotton Incorporated research group at the national level. We have also directly provided written bulletins distributed by Cotton Incorporated and by Texas A&M University and New Mexico State University, plus powerpoint presentations for use by others as basic information for producers and seed company representatives. Information from Race 4 Fusarium disease resistance screening trials and variety trials is also sent out through via web based communications using the University of California cotton web site (https://cottoninfo.ucdavis.edu). Updates on disease resistance evaluations and variety screening work for fiber quality and other characteristics are also part of multi-state efforts with a primary USDA-ARS research partner located in Lubbock, TX and through seed company representatives who submit varieties for evaluation in these variety and disease screening trials. This participation provides information to breeders of potential use in selection and improvement programs. The project PI also serves as a coordinator for a multi-state effort in cooperation with USDA-ARS to test Pima cotton in 3 locations across parts of the U.S, which then collects fiber and seed quality and yield data. This data is available in a data set managed by the USDA-ARS out of Stoneville, MS. Results from deficit irrigation trials and drip irrigation management trials provide information on both Pima and Upland cotton responses to deficit irrigation, with information made available to growers, irrigation consultants, seed companies, and agency representatives dealing with crop production under limited irrigation water situations, which seem to have become more commonplace in recent years in the western U.S. Changes/Problems:Extra expenses associated with operations under COVID-19 restrictions have come in the form of: (1) new protocols that put limits on staff work locations and duties, particularly those that formerly were done by a team of multiple people; (2) PPE requirements and associated costs, and training; and (3) extra vehicles required for transport to all work sites, since no more than one person is allowed to travel per vehicle. While necessary, these requirements and changes in work environments and methods have greatly increased operating costs and will limit our flexibility to complete tasks within our current operating budgets. For all of our University approved research projects in 2020, we were required to document our work plans and changes in operations under COVID-19 protocols, and submit all of them for upper administration review and approvals. While most projects were eventually approved for 2020, the extra steps and extra expenses have made operations more difficult and resulted in some delays in data collection and analysis. In recent years, we have not been able to identify grower-cooperators sites for Upland / Acala variety trials due to low commodity prices for upland cotton and reduced acreages other than for seed production. Both of these factors have reduced the willingness of growers to take on the extra expense and disruption of their operations that occur with large scale variety trials. We have utilized facilities of the University of CA West Side University research center for all of the Upland cotton variety trial work in 2015 through 2020, and expect that the same situation will prevail in 2021 for Upland variety trials. We will continue to conduct the trials at the University of CA research center at West Side REC, although prices for recharge on the research centers are also going up and these type of issues may limit some of our capabilities to conduct these trials in future years. On a positive note for smaller scale field research trials that require machine harvest yield data, the CA cotton industry and my program provided some funding to make major modifications to our small plot cotton spindle picker for 2020, which was in need of major changes to meet safety requirements of the University which put those field harvest capabilties at serious risk of curtailment prior to these successful modifications. For the Fusarium race 4 evaluations, retention of and maintenance of two field sites has been expensive since we need to pay growercooperators at these sites due to the extreme amount of damage to yield potentials in these severe disease sites. So far the CA cotton industry has assisted with helping cover grower/cooperator supplemental costs and losses associated with the research in the form of a supplemental grant. but this requirement could be problematic in future years if cotton acreage continues to decline and research funds become tighter. The impact of finding FOV-4 disease in cotton in west Texas may have an impact on future options and interest in continuing FOV-4 research in California, but for now our Texas-based USDA-ARS cooperator and California State University Fresno cooperator have indicated that we will plan to continue these field efforts in 2021. What opportunities for training and professional development has the project provided?Variety trial work, disease resistance evaluations, and the deficit irrigation projects were mostly operated and conducted utilizing Staff Research Associates of the University of California and student Field Assistants hired through the UC Davis Plant Sciences Department. Since our research sites and field facilities are located in the San Joaquin Valley of California, the students and other part time help utilized in the project are community college students, students at California State University Fresno or CSU Bakersfield, rather than University of CA students. The field and laboratory work provide opportunities for all staff to become acquainted with field research techniques, requirements for accuracy and care in operating field trials and collecting data, and improved methods of data management. Safety training is also provided, which is important in dealing with on-farm research both in private farm and university settings. Fusarium resistance evaluations mostly in the field, but also in greenhouse settings have been used for student and industry training sessions on identification and screening methods for improving grower, consultant and researcher understanding of what to look for and how to deal with the disease race 4 Fusarium if found in cotton fields. Most of this training was in the San Joaquin Valley of California, but additional information efforts (verbal presentations, written materials or Powerpoint presentations and photos) have also been provided to researchers in other states, including Arizona, New Mexico, Texas and Georgia during the reporting period. Continuing field screening for disease resistance to Fusarium oxysporum race 4 has involved entries provided by various associations with our USDA-ARS cooperator (M. Ulloa) as well as a broad cross section of participating seed companies who provide entries for field evaluations, including advanced lines from breeders all across the US production region. In 2019, the general screening of experimental varieties and commercially available germplasm continued in both public entry evaluations (from companies and private breeders) and also, screening for FOV-4 response of different cultivars provided through public breeders associated with Universities and USDA continued through the Regional Breeder Testing Network entries (RBTN program). These FOV-4 screening evaluations were done primarily in California, but with the assistance of our USDA-ARS breeder/geneticist cooperator, additional screening was done in FOV-4 infested fields in far-west Texas. Several short videos covering aspects of Fusarium race 4 disease symptoms in plants and parameters evaluated in disease screenings have been posted on the University of CA cotton website (http://cottoninfo.ucdavis.edu), with the purpose of providing industry, growers and their representatives with training in recognizing the disease symptoms in the field. How have the results been disseminated to communities of interest?Information delivery has changed due to COVID-19 restrictions and limitations on our activity due to University limits. During the period, information on Fusarium race 4 symptomology, spread of the pathogen, recommended management and containment practices, and results of host plant resistance screening programs has been disseminated using zoom presentation (at 3 industry meetings) as well as through posting of information on UC cotton website. Information was also provided in written form (Guidelines, suggestions for field scouting) and as disease photo identification materials made available to Cooperative Extension representatives in Texas, New Mexico and Arizona as requested. One brochure covering issues with Fusarium was updated in cooperation with USDA-ARS, Cotton Incorporated, Texas A&M University, and New Mexico State University; and an update of a brochure / bulletin on FOV-4 identification and management practices was produced and printed by the CA Cotton Growers Association. For the UC brochure, the project PI provided the text used in the bulletin, some of the photos, and review of the final product. Presentations were made at national meetings at Beltwide Cotton Conferences in Austin (2020) prior to the COVID-related limits, During the reporting period. In California, variety trial yield and quality information, and Fusarium information has been provided using the University of CA Cotton web site:(http:// cottoninfo.ucdavis.edu) and through industry and commodity group websites, such as that of the CA Cotton Growers and Ginners Association as another means of information dissemination. Other written materials have been provided on both variety trial results (at the California Cotton Growers and Ginners Association) and Fusarium disease resistance screening to industry representatives on request and at three meetings during the year to the CA Cotton Alliance board and at the quarterly meetings of the CA Cotton Growers and Ginners Association board. Research and data summaries were presented and discussed via website postings available to growers, field agronomists and plant pathologists. What do you plan to do during the next reporting period to accomplish the goals?The same type of field and laboratory work is expected to continue in 2020-2021 efforts in: (1) variety evaluations (growth, yield, fiber quality) for Upland, Acala and Pima types of cotton, both in research center trials and grower field sites (2) nitrogen management and nitrogen uptake trials in Pima and Upland cotton witll continue, with the nitrogen management trial again conducted both under (a) furrow irrigation management / dry fertilizer application regimes; and (b) subsurface drip irrigation / liquid, injected high frequency fertilization regimes. The 2021 field year is expected to be the final year of a three year set of funded field trials, with the fourth year being planned for data analysis and further reporting. (3) field and greenhouse evaluations of selections and new entries from our USDA Cooperators and seed companies will continue in 2020-2021, with expected locations in two counties where we have identified disease infested sites for the screenings. This work will be conducted as in recent years, with field evaluations utilizing a large crew for management of the research plots and for data collection at intervals through the season at both test sites plus the greenhouses. Seed increase efforts for selections will continue at UC West Side REC and UC Kearney REC locations (field and greenhouse, respectively). We will continue to evaluate possible funding sources and adequacy of staff and resources to conduct further irrigation management/water stress studies or responses to saline irrigations if funding sources can be identified.
Impacts What was accomplished under these goals?
Per the objectives for this project, accomplishments and progress made in the reporting period included: (1) San Joaquin Valley multi-site variety evaluations to provide assessments of relative growth and maturity habits, yield and fiber quality under a range of soil, salinity and irrigation conditions at Research Center and grower field sites. Field research, including both University of CA Research Center and on-farm trials were utilized to evaluate and provide information to growers, seed companies, breeders and industry personnel regarding variety choice options and other crop management options on cotton growth, yield and fiber quality for Pima, Upland and Acala types of cotton. Research Center Pima, Upland and Acala cotton variety trials and on- farm Pima variety trials focused on yield performance, disease resistance/tolerance (Fusarium wilt and Verticillium wilt), and fiber quality components. This information is utilized widely by seed companies for decisions on regional cotton development and choices, and by growers and associated industry in making production decisions. (2) We are conducting a nitrogen management trial with major funding from the CA Dept of Food and Agriculture FREP program, with supplemental funding from CA Cotton Growers and Ginners Association to evauate nitrogen (N) utilization, uptake and removal with harvest, focused mostly on modern Pima cotton cultivars, including impacts of drip versus flood/furrow irrigation on these measures of utilization. The 2020 production year was the second field research year for this project. A large multi-application level nitrogen fertilizer management trial was conducted with multiple Upland varieties and multiple Pima cotton varieties at the UC West Side REC site, with multiple nitrogen application treatments ranging from 0 to 230 lbs of N/acre, adjusted for residual soil nitrate in the upper root zone. Plant samples were collected at peak biomass production to assess plant partitioning of nitrogen, and seedcotton samples were collected from plots at harvest to determine nitrogen removal with harvest, including separation into seed, lint, and gin trash components. At harvest time at four on-farm Pima variety trial locations across the San Joaquin Valley, whole plant samples and harvest samples were also collected to evaluate partitioning and harvest removal of nitrogen in grower field conditions, different locations and soils. The plant and soil samples have been submitted for analyses, but results have not been provided to date. A third year of the same type of trials are planned for 2021 production year, and results from the nitrogen response trials and nitrogen harvest removal evaluationw will be provided to the industry and to the CA Dept of Food and Agriculture Fertilizer Research and Education program as part of outreach when analyses are completed. . (3) Greenhouse and field evaluations were continued in assessments of relative varietal differences in susceptibility to race 4 of the fungal pathogen Fusarium oxysporum f. spp. vas infectum; including cooperative efforts with a USDA-ARS geneticist and multiple state and international partners to make and test crosses, and evaluate selections for improved tolerance or resistance in Pima and Upland cotton cultivars to race 4 Fusarium oxysporum f. spp. vas infectum. Information on varietal susceptibility and relative mortality due to disease was produced, indicating existence of highly susceptible and highly-resistant varieties in Pima, including commercially-available and experimental entries. 2020 disease resistance screening of commercial seed company/breeder cultivars was accomplished and is being summarized for both FOV-4 and verticillium screening sites in a program aimed at providing independent disease resistance screening, and made available in written form and will also be accessible via a University of CA cotton website (https://cottoninfo.ucdavis.edu) later in January, 2021. We continue to provide field assistance and testing locations for our part of a long-term breeding program conducted in coordination with Dr. Mauricio Ulloa of the USDA-ARS (Lubbock, TX), with this effort directed toward evaluations of host plant resistance to race 4 Fusarium oxysporum in cotton. This work over multiple years has produced a collection of higher resistance experimental selections and crosses that in multiple years (continuing in 2020) have: (1) been grown out for continued evaluation in one or two disease infested fields per year in CA; (2) been evaluated in Texas by researchers in 2019 and 2020 for host plant resistance screens race 4 Fusarium-infested fields in far West Texas; and (3) additional field plots were grown out to increase seed supplies for evaluation of selections with good evidence of improved Fusarium race 4 resistance for further field and greenhouse selection screenings. No new projects were initiated in the reporting period involving water stress or irrigation water salinity responses of cotton cultivars (Upland or Pima) due to limited funding approved for the projects, and personnel limitations due to retirements and lack of new funding for this work.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Ulloa, M., Hutmacher, R.B., Schramm, T. et al. Sources, selection and breeding of Fusarium wilt (Fusarium oxysporum f. sp. vasinfectum) race 4 (FOV4) resistance in Upland (Gossypium hirsutum L.) cotton. Euphytica 216, 109 (2020). https://doi.org/10.1007/s10681-020-02643-5
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Diaz, J., Garcia, J., Lara, C., Hutmacher, R.B., Ulloa, M., Nichols, R.L., Ellis, M.L. 2020. Characterization of current Fusarium oxysporum f. sp. vasinfectum isolates from cotton in the San Joaquin Valley of California and Lower Valley El Paso Texas. Plant Disease - First Look. 6 October, 2020. https://doi.org/10.1094/PDIS-05-20-1038-RE
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2020
Citation:
Ellis, M.L., Garcia, J., Lara, C., Hutmacher, R.B., Ulloa, M., Nichols, R.L. 2020. Genotypic Characterization of Current Field Populations of Fusarium oxysporum f. sp. vasinfectum isolates from California. In: Proc. Beltwide Cotton Conferences, Austin, TX, Jan. 8-1-, 2020.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2020
Citation:
Ellis, M.L., Diaz, J.,Hutmacher, R.B., Ulloa, M. 2019. The Past and Current Impact of Fusarium oxysporum f. sp. vasinfectum on cotton production in California. National Cotton Council, Proc. Beltwide Cotton Conf., New Orleans, LA.
- Type:
Other
Status:
Published
Year Published:
2019
Citation:
Identification and Management of Fusarium Wilt Race 4 of Cotton in Texas and New Mexico. 2019.
T. Isakeit, M. Ulloa, R.B. Hutmacher, J. Idowu, R.L. Nichols; Cotton Incorporated web publication. https://www.cottoninc.com/cotton-production/ag-research/plant-pathology/identification-and- management-of-fusarium-wilt-race-4/ UPDATED 2019.
- Type:
Conference Papers and Presentations
Status:
Accepted
Year Published:
2020
Citation:
Lawrence, K., Hagan, A., Norton, R., Hu, J., Faske, T., Hutmacher, R.B., Mueller, J., Small, I., Grabau, Z., Kemerait, B., Jardine, D., Price, P., Allen, T., Meeks, C., Idowu, J., Thiessen, L., Byrd, S., Goodson, J., Kelly, H., Wheeler, T., Isakeit, T., Mehl, H. 2020. Cotton Disease Loss Estimate Committee Report, 2019. National Cotton Council, Proc. Beltwide Cotton Conf., Austin, TX, Jan. 8-10, 2020.
- Type:
Websites
Status:
Other
Year Published:
2020
Citation:
Hutmacher, R.B., Munk, D., Marsh, B.H., Weir, B.L., Keeley, M., Angeles, J., Frigulti, T. 2020. Lint Yields, Turnouts - Pima and Upland Cotton Variety Trials, San Joaquin Valley of California: 2019 Field Trials. University of California Division of Agriculture and Nat. Resources. website: https://cottoninfo.ucdavis.edu, 11 pp.
- Type:
Websites
Status:
Other
Year Published:
2020
Citation:
Hutmacher, R.B., Munk, D., Marsh, B.H., Weir, B.L., Keeley, M., Angeles, J., Frigulti, T. 2020. Fiber Quality Analyses - Pima and Upland Cotton Variety Trial, San Joaquin Valley of California: 2019 Field Trials. University of California Division of Agriculture and Nat. Resources. website: https://cottoninfo.ucdavis.edu, 11 pp.
- Type:
Websites
Status:
Other
Year Published:
2020
Citation:
Hutmacher, R.B., Munk, D., Marsh, B.H., Weir, B.L., Keeley, M., Angeles, J., Frigulti, T. 2020. Fusarium Disease Screening Analyses for Pima and Upland Cotton Varieties: Multi-Site Trials 2019, San Joaquin Valley of California: Field Screening Results. University of California Division of Agriculture and Nat. Resources. website: https://cottoninfo.ucdavis.edu, 11 pp.
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