Source: COLD SPRING HARBOR LABORATORY ASSOCIATION, INC submitted to NRP
COLD SPRING HARBOR LABORATORY 2019 WORKSHOP ON CEREAL GENOMICS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1021106
Grant No.
2020-67013-30712
Cumulative Award Amt.
$25,000.00
Proposal No.
2019-05363
Multistate No.
(N/A)
Project Start Date
May 1, 2020
Project End Date
Apr 30, 2021
Grant Year
2020
Program Code
[A1141]- Plant Health and Production and Plant Products: Plant Breeding for Agricultural Production
Recipient Organization
COLD SPRING HARBOR LABORATORY ASSOCIATION, INC
1 BUNGTOWN RD
COLD SPRING HARBOR,NY 11724-2209
Performing Department
(N/A)
Non Technical Summary
The Cereal Genomics Workshop plans to give plant biologists and breeders the skills necessary to navigate the increasingly complex genomics information landscape, and to identify and encourage young researchers who might choose plant genomics as a career by exposing them to the field's practice and practitioners.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20115991081100%
Keywords
Goals / Objectives
This proposal requests funds to support the participation of US graduate students in plant genetics and breeding to a training course in cereal genomics. A major aim of this course will be to enable students and post docs to take advantage of the emerging genomic data in the cereals, including complete genome sequences of all major cereal crops and model systems, and become trained in methods including Next Gen gene expression analysis, genome wide association analysis and genome editing. The proposal is targeted at the "Plant Breeding for Agricultural Production" priority area- since it will train students in genomics techniques that can enable plant breeding. Specific parts of the workshop are devoted to quantitative genetics, genome analysis, genome editing and phenomics, major areas of interest to current and future breeding efforts. It will also enable graduate student interaction with geneticists and other scientists, to promote careers in plant breeding, genetics and genomics. As a follow up to the highly successful Cereal Genomics workshops held in 2005, 2009 and 2016 at CSHL, we propose to organize this week-long workshop in the fall of 2019. With the recent releases of many cereal genome sequences, availability of huge GWAS and phenomics datasets and the emerging revolutionary genome editing technologies, the time is ripe to train young students and scientists in the tools and skills they need to exploit the emerging genome data across all cereal crops. The workshop is geared toward scientists from graduate, post-doctoral, faculty and industry levels, and will teach state of the art methodologies in cereal crop genomic analysis and data processing. Topics will include Cereal Anatomy and Diversity, Databases, Molecular Breeding, QTL/ GWAS, Phenomics Genome Sequencing and Structure, mRNAseq Analysis, Genome Editing and applications of Cereal Genomics. The format will be morning and evening lectures and afternoon hands on labs in informatics, using resources at CSHL and Cyverse. It is an ideal time for this new workshop, because of the recent advances in whole genome sequencing, genome editing and related fields. In addition, the general trend in funding geared towards crop plants means that many labs are trying to re-tool. This workshop will serve as a catalyst and important training forum to disseminate new techniques and promote use of data from other federally funded projects.
Project Methods
The workshop is being organized by a core group of three workshop directors, who will attend the whole workshop to ensure continuity and provide support where needed: Dave Jackson, Professor, Cold Spring Harbor Lab., NYSarah Hake, Director, Plant Gene Expression Center, Albany, CA 94710; Full Adjunct Professor,Plant and Microbial Biology Dept., University of California, Berkeley.Doreen Ware, Associate Professor, Cold Spring Harbor Lab., NYThe workshop will run on a template of the existing, highly successful CSHL courses. The short duration of the workshop and its contemporary content makes it both attractive and valuable to established investigators as well as to advanced graduate students and postdoctoral fellows. The three core workshop directors will attend the entire workshop to ensure continuity between the different presentations. Approximately 12 invited lecturers will give lectures on topics in cereal biology, genetics and genomics, combined with practical computer sessions that reinforce the lectures by emphasizing problem solving and skill development. A typical day will consist of two 2-hour lectures delivered at 9:00 AM and 7:00 PM. The afternoons will consist of a 4-5 hour "hands on" sessions that will be a combination of "wet lab" (observing phenotypes, collecting data) and computer sessions centered around problem sets designed and supervised one of the days' lecturers. The problem sets will present students with typical tasks in cereal genome data manipulation. After the completion of the problem set, the lecturer will meet with the students to review the problems and to discuss any unresolved issues.This workshop will provide both "wet lab" and in silico training, and the lab will be set up so that each student has a laptop. "Wet lab" training will be provided in the comparative anatomy, phenomics, CRISPR and QTL sections, where students will examine samples and make measurements for statistical analysis. The faculty (instructors and lecturers- see list, below) represent active researchers in cereal genetics and genomics who have made significant contributions to the field. This will ensure that the newest techniques and ideas are presented. The seminar schedule will also be circulated throughout Cold Spring Harbor Laboratory to facilitate attendance by CSHL scientists, thereby increasing the interaction between workshop participants and the resident staff.

Progress 05/01/20 to 03/29/21

Outputs
Target Audience:PARTICIPANTS: *Christian Cantos, MS, Graduate Student, Penn State University, Department of Biology, University Park, PA. Lab Head: Dr. SarahAssmann Jeongmin Choi, PhD, Postdoctoral Fellow, University of Cambridge, Department of Plant Sciences, Cambridge, UK. Lab Head: Dr. Uta Paszkowski *Anju Giri, PhD, Postdoctoral Fellow, Cornell University, Institute of Genomic Diversity, Ithaca, NY. Lab Head: Dr. Edward Buckler Yasmeen Haider, Graduate Student, Arcadia Biosciences, Research and Development, Davis, CA. Lab Head: Dr. Randy Schultz Sessen Iohannes, Graduate Student, Sant'Anna School of Advanced Studies, Agricultural Sciences and Biotechnology, Pisa, Italy. Lab Head: Mario Enrico Pe' Emmanuel Iwuala, MS, Graduate Student, University of Lagos, Botany, Lagos, Nigeria.Lab Head: Dr. Caroline Umebese *Sunil Kumar Kenchanmane Raju, PhD, Postdoctoral Fellow, Michigan State University, Plant Biology, East Lansing, MI. Lab Head: Dr. Chad Niederhuth Hyun-Sook Lee, PhD, Postdoctoral Fellow, Department of Agronomy, Chungnam National University, Daejeon, South Korea. Lab Head: Dr. Sang-Nag Ahn Cathal Meehan, Graduate Student, University of Warwick, School of Life Sciences, Coventry, UK. Lab Head: Dr. Jose Gutierrez-Marcos *Queen Obi, MS, Staff Scientist, International Institute of Tropical Agriculture, Bioscience Unit, Ibadan, Nigeria. Lab Head: Dr. Melaku Gedil *Jeremy Pardo, Graduate Student, Michigan State University, Plant Biology, East Lansing, MI. Lab Head: Dr. Robert VanBuren Pratishtha Poudel, MS, Graduate Student, Oklahoma State University, Plant and Soil Sciences, Stillwater, OK. Lab Head: Dr. Phillip Alderman Curtis Provencher, MS, Staff Scientist, Inari Agriculture, Digital, Cambridge, MA.Lab Head: Dr. Alex Wimbush Rency Raquid, Graduate Student, International Rice Research Institute, Strategic Innovation Platform, Los Baños, Laguna, Philippines. Lab Head: Dr. Jae Sung Lee Armin Patrick Scheben, PhD, Postdoctoral Fellow, Cold Spring Harbor Laboratory, Simons Center for Quantitative Biology, Cold Spring Harbor, NY. Lab Head: Dr. Adam Siepel *Salome Suarez, MS, Graduate Student, Oklahoma State University, Entomology and Plant Pathology, Stillwater, OK. Lab Head: Dr. Stephen Marek 16 Participants (8 Female, 8 Male, 1 URM) * USDA Scholarship support Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Students were trained in modern genomics techniques relevant to plant breeding and crop improvement. The small format of the workshop made it easy for students to network with the instructors, and discussions continued throughout mealtimes and after the lectures. Students gained in depth understanding of bioinformatics methods, and all data from the workshop, and workflows are permanently stored on Cyverse. Effectiveness of the workshop was assessed using a surveyfilled out by students. The responses were unanimously very positive ("exceptional" or "very good"), with statements such as: "welcoming environment, helpful instructors"...."inclusion of several instructors pioneering in the field"..... "diversity of participants"...."really life changing stuff".... "meeting world class leaders in research"... "carefully chosen topics that gave a very broad overview of field". From past experience, we find that students who have attended these workshops gain confidence and self-motivation, and a close bond with other students and instructors, important for future careers and networking. This workshop was a great success, and we believe it will be game changing for the students' careers. How have the results been disseminated to communities of interest?The workshop materials are freely available through Cyverse, for anyone who has a (free) CyVerse account in the Discovery Environment What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? This one-week workshop in Cereal Genomics enabled participants to take advantage of emerging genetic tools and the completed cereal genome sequences of most of the major cereal crops. The workshop featured morning and evening lectures with afternoon lab exercises, including hands-on lab work and computer sessions in comparative anatomy, GWAS, Next Gen expression analysis, whole genome sequencing assembly, emerging model systems, genome editing and phenomics. The faculty (instructors and invited lecturers) were/are active researchers in cereal genetics and genomics who have made significant contributions to the field, ensuring that the latest techniques and ideas were presented. The course was structured to provide time for informal discussions and exchange with instructors. Topics Included: Comparative anatomy and phylogeny Cereal genomes, assembly, annotation and synteny Genetics and databases Quantitative trait locus mapping and genome wide association studies Genome wide expression analyses Reverse genetics and genome editing Phenomics SEMINARS: Edward Buckler, USDA-ARS, Ithaca, NY "Nucleotide level natural variation and genetic architecture" "QTL Mapping and Prediction" "Machine learning the central dogma of molecular biology" 'Learning from evolution and molecular biology for a new agriculture" Katrien Devos, University of Georgia, Augusta, GA "Genetic analysis of non-model plants: challenges and successes" Jorge Dubcovsky, University of California Davis, Davis, CA "Resources for Wheat Functional Genomics" Andrea Eveland, Donald Danforth Plant Science Center, St. Louis, MO "Extracting biological insights through multi-omics data integration" Elizabeth Kellogg, Donald Danforth Plant Science Center, St. Louis, MO "Introduction to the grass family" Sam Leiboff, University of California, Berkeley, Albany, CA "Hands-on RNAseq: from maize sequence to developmental inference" Devin O'Connor, Pairwise Plants, Research Triangle Park, NC "Enabling comparative biology with comparative genomics tools" Uta Paszkowski, University of Cambridge, UK "Molecular Genomics of arbuscular mycorrhizal symbiosis in cereals" Christopher Topp, Donald Danforth Plant Science Center, St. Louis, MO "Plant Phenomics" Bing Yang, University of Missouri, Columbia, MO "Reverse Genetics & Genome Editing"

Publications


    Progress 05/01/20 to 11/23/20

    Outputs
    Target Audience:PARTICIPANTS: *Christian Cantos, MS, Graduate Student, Penn State University, Department of Biology, University Park, PA. Lab Head: Dr. SarahAssmann Jeongmin Choi, PhD, Postdoctoral Fellow, University of Cambridge, Department of Plant Sciences, Cambridge, UK. Lab Head: Dr. Uta Paszkowski *Anju Giri, PhD, Postdoctoral Fellow, Cornell University, Institute of Genomic Diversity, Ithaca, NY. Lab Head: Dr. Edward Buckler Yasmeen Haider, Graduate Student, Arcadia Biosciences, Research and Development, Davis, CA. Lab Head: Dr. Randy Schultz Sessen Iohannes, Graduate Student, Sant'Anna School of Advanced Studies, Agricultural Sciences and Biotechnology, Pisa, Italy. Lab Head: Mario Enrico Pe' Emmanuel Iwuala, MS, Graduate Student, University of Lagos, Botany, Lagos, Nigeria.Lab Head: Dr. Caroline Umebese *Sunil Kumar Kenchanmane Raju, PhD, Postdoctoral Fellow, Michigan State University, Plant Biology, East Lansing, MI. Lab Head: Dr. Chad Niederhuth Hyun-Sook Lee, PhD, Postdoctoral Fellow, Department of Agronomy, Chungnam National University, Daejeon, South Korea. Lab Head: Dr. Sang-Nag Ahn Cathal Meehan, Graduate Student, University of Warwick, School of Life Sciences, Coventry, UK. Lab Head: Dr. Jose Gutierrez-Marcos *Queen Obi, MS, Staff Scientist, International Institute of Tropical Agriculture, Bioscience Unit, Ibadan, Nigeria. Lab Head: Dr. Melaku Gedil *Jeremy Pardo, Graduate Student, Michigan State University, Plant Biology, East Lansing, MI. Lab Head: Dr. Robert VanBuren Pratishtha Poudel, MS, Graduate Student, Oklahoma State University, Plant and Soil Sciences, Stillwater, OK. Lab Head: Dr. Phillip Alderman Curtis Provencher, MS, Staff Scientist, Inari Agriculture, Digital, Cambridge, MA.Lab Head: Dr. Alex Wimbush Rency Raquid, Graduate Student, International Rice Research Institute, Strategic Innovation Platform, Los Baños, Laguna, Philippines. Lab Head: Dr. Jae Sung Lee Armin Patrick Scheben, PhD, Postdoctoral Fellow, Cold Spring Harbor Laboratory, Simons Center for Quantitative Biology, Cold Spring Harbor, NY. Lab Head: Dr. Adam Siepel *Salome Suarez, MS, Graduate Student, Oklahoma State University, Entomology and Plant Pathology, Stillwater, OK. Lab Head: Dr. Stephen Marek 16 Participants (8 Female, 8 Male, 1 URM) * USDA Scholarship support Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Students were trained in modern genomics techniques relevant to plant breeding and crop improvement. The small format of the workshop made it easy for students to network with the instructors, and discussions continued throughout mealtimes and after the lectures. Students gained in depth understanding of bioinformatics methods, and all data from the workshop, and workflows are permanently stored on Cyverse. Effectiveness of the workshop was assessed using a surveyfilled out by students. The responses were unanimously very positive ("exceptional" or "very good"), with statements such as: "welcoming environment, helpful instructors"...."inclusion of several instructors pioneering in the field"..... "diversity of participants"...."really life changing stuff".... "meeting world class leaders in research"... "carefully chosen topics that gave a very broad overview of field". From past experience, we find that students who have attended these workshops gain confidence and self-motivation, and a close bond with other students and instructors, important for future careers and networking. This workshop was a great success, and we believe it will be game changing for the students' careers. How have the results been disseminated to communities of interest?The workshop materials are freely available through Cyverse, for anyone who has a (free) CyVerse account in the Discovery Environment What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

    Impacts
    What was accomplished under these goals? This one-week workshop in Cereal Genomics enabled participants to take advantage of emerging genetic tools and the completed cereal genome sequences of most of the major cereal crops. The workshop featured morning and evening lectures with afternoon lab exercises, including hands-on lab work and computer sessions in comparative anatomy, GWAS, Next Gen expression analysis, whole genome sequencing assembly, emerging model systems, genome editing and phenomics. The faculty (instructors and invited lecturers) were/are active researchers in cereal genetics and genomics who have made significant contributions to the field, ensuring that the latest techniques and ideas were presented. The course was structured to provide time for informal discussions and exchange with instructors. Topics Included: Comparative anatomy and phylogeny Cereal genomes, assembly, annotation and synteny Genetics and databases Quantitative trait locus mapping and genome wide association studies Genome wide expression analyses Reverse genetics and genome editing Phenomics SEMINARS: Edward Buckler, USDA-ARS, Ithaca, NY "Nucleotide level natural variation and genetic architecture" "QTL Mapping and Prediction" "Machine learning the central dogma of molecular biology" 'Learning from evolution and molecular biology for a new agriculture" Katrien Devos, University of Georgia, Augusta, GA "Genetic analysis of non-model plants: challenges and successes" Jorge Dubcovsky, University of California Davis, Davis, CA "Resources for Wheat Functional Genomics" Andrea Eveland, Donald Danforth Plant Science Center, St. Louis, MO "Extracting biological insights through multi-omics data integration" Elizabeth Kellogg, Donald Danforth Plant Science Center, St. Louis, MO "Introduction to the grass family" Sam Leiboff, University of California, Berkeley, Albany, CA "Hands-on RNAseq: from maize sequence to developmental inference" Devin O'Connor, Pairwise Plants, Research Triangle Park, NC "Enabling comparative biology with comparative genomics tools" Uta Paszkowski, University of Cambridge, UK "Molecular Genomics of arbuscular mycorrhizal symbiosis in cereals" Christopher Topp, Donald Danforth Plant Science Center, St. Louis, MO "Plant Phenomics" Bing Yang, University of Missouri, Columbia, MO "Reverse Genetics & Genome Editing"

    Publications