Progress 06/01/19 to 04/20/21
Outputs
Target Audience:Target audience is the scientific community, specifically plant biologists, who will be targeted with presentations and publications. This was done specifically as oral presentations to the virtual ASPB Plant Biology 2020 conference and Maize Genetics 2020 virtual conference. Several publications have resulted from this project, listed in "products". General public was targeted via scientific publications, press releases, and outreach on Twitter, LinkedIn, and public talks. Changes/Problems:This project was terminated prior than the initial completion date (initial date 5/31/2021) on 12/18/2020 due to the graduation of the principal investigator, Katherine Murphy, and the early completion of the project goals. This graduation was earlier than anticipated due to early goal completion, covid-19 restrictions, and the future employment of Katherine Murphy. What opportunities for training and professional development has the project provided?I was trained through bi-weekly (virtual) meetings with my advisor, Philipp Zerbe. I performed virtual informational interviews with professionals in the plant biology community, as well as hosted a webinar panel on careers in plant biology startups. I participated in virtual networking sessions and multiple virtual conferences. I also participated in the writing and presenting on the results, which is paramount to my communication training. Given covid-19 restrictions, all professional development was done virtually. How have the results been disseminated to communities of interest?Results were disseminated via several peer-reviewed publications, two conference publications, a thesis, and numerous online talks. I further promoted these results via LinkedIn, Twitter, and my personal website. For a general audience, I disseminated the results via public internet talks and social media. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
I made progress towards all three major goals of this project, completing them or otherwise finding them not possible to complete within the scope of this project. For Goal 1) In addition to the previous report, I elucidated the developmental and spatial formation and distribution of dolabralexins using new, additional techniques to those originally proposed. For Goal 2), I elucidated the structure-activity principles underlying dolabralexin antifungal activity and their in planta defensive properties. For Goal 3) I completed assessment of pathogen resistance assays using major Fusarium pathogens in vitro, and attempted, without success, to transform the pathway into tomato. These findings were or are in preparation for peer-reviewed publication.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Ding, Yezhang, et al. "Genetic elucidation of interconnected antibiotic pathways mediating maize innate immunity." Nature Plants (2020): 1-14.
- Type:
Journal Articles
Status:
Accepted
Year Published:
2020
Citation:
Murphy, K. M., et al. (2020). "Bioactive Diterpenoids Impact the Composition of the Root-Associated Microbiome in Maize (Zea mays)." Scientific Reports. Accepted.
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2020
Citation:
Murphy, K.M. "Form and Function of Diterpenoids in Maize (Zea mays)" Thesis, UC Davis (2020).
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2020
Citation:
Murphy, K.M. (2020) "Diterpenoid-deficient maize mutant has an altered rhizosphere microbiome." ASPB Plant Biology 2020. Oral Presentation.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2020
Citation:
Murphy, K.M. (2020) "Diterpenoid-deficient maize mutant has an altered rhizosphere microbiome." Maize Genetics Conference 2020. Oral Presentation.
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Progress 06/01/19 to 01/05/21
Outputs
Target Audience:Target audience is the scientific community, specifically plant biologists, who will be targeted with presentations and publications. This was done specifically as oral presentations to the virtual ASPB Plant Biology 2020 conference and Maize Genetics 2020 virtual conference. Several publications have resulted from this project, listed in "products". General public was targeted via scientific publications, press releases, and outreach on Twitter, LinkedIn, and public talks. Changes/Problems:This project was terminated prior than the initial completion date (initial date 5/31/2021) on 12/18/2020 due to the graduation of the principal investigator, Katherine Murphy, and the early completion of the project goals. This graduation was earlier than anticipated due to early goal completion, covid-19 restrictions, and the future employment of Katherine Murphy. What opportunities for training and professional development has the project provided?I was trained through bi-weekly (virtual) meetings with my advisor, Philipp Zerbe. I performed virtual informational interviews with professionals in the plant biology community, as well as hosted a webinar panel on careers in plant biology startups. I participated in virtual networking sessions and multiple virtual conferences. I also participated in the writing and presenting on the results, which is paramount to my communication training. Given covid-19 restrictions, all professional development was done virtually. How have the results been disseminated to communities of interest?Results were disseminated via several peer-reviewed publications, two conference publications, a thesis, and numerous online talks. I further promoted these results via LinkedIn, Twitter, and my personal website. For a general audience, I disseminated the results via public internet talks and social media. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
I made progress towards all three major goals of this project, completing them or otherwise finding them not possible to complete within the scope of this project. For Goal 1) In addition to the previous report, I elucidated the developmental and spatial formation and distribution of dolabralexins using new, additional techniques to those originally proposed. For Goal 2), I elucidated the structure-activity principles underlying dolabralexin antifungal activity and their in planta defensive properties. For Goal 3) I completed assessment of pathogen resistance assays using major Fusarium pathogens in vitro, and attempted, without success, to transform the pathway into tomato. These findings were or are in preparation for peer-reviewed publication.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Ding, Yezhang, et al. "Genetic elucidation of interconnected antibiotic pathways mediating maize innate immunity." Nature Plants (2020): 1-14.
- Type:
Journal Articles
Status:
Accepted
Year Published:
2020
Citation:
Murphy, K. M., et al. (2020). "Bioactive Diterpenoids Impact the Composition of the Root-Associated Microbiome in Maize (Zea mays)." Scientific Reports. Accepted.
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2020
Citation:
Murphy, K.M. "Form and Function of Diterpenoids in Maize (Zea mays)" Thesis, UC Davis (2020).
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2020
Citation:
Murphy, K.M. (2020) "Diterpenoid-deficient maize mutant has an altered rhizosphere microbiome." ASPB Plant Biology 2020. Oral Presentation.
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2020
Citation:
Murphy, K.M. (2020) "Diterpenoid-deficient maize mutant has an altered rhizosphere microbiome." Maize Genetics Conference 2020. Oral Presentation.
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Progress 06/01/19 to 05/31/20
Outputs
Target Audience:Scientific community, via presentations and publications, specifically the Maize Genetics Community and the American Society of Plant Biologists. I presented my work at ASPB Plant Biology 2019 Conference lightening talk and poster, as well as shared publications via LinkedIn and Twitter. General public, via scientific publications and press releases on the published research. I have presented via iBiology and shared updates on my personal blog for education of the general public. Undergraduate students, via laboratory training, including students underrepresented. I have and continue to mentor 4 undergraduate students in the lab through this project. Changes/Problems:One change is due to the coronavirus, which caused the cancellation of the Maize Genetics Meeting in March 2020, which I had proposed and planned to attend as part of this fellowship and had been invited to give a talk. Instead, I will present my talk via the web and attempt to attend another conference in the future once it is safe to travel. A problem encountered during this project was the failure to transform tomato with the biosynthetic pathway of interest. Technical difficulties at the UC Davis Transformation Facility prevented obtainment of tomato transformants for further experimentation, as was proposed in this project. This experimental aim could not be continued. A second problem occurred during MALDI imaging with Trent Northen's lab at the Joint Genome Institute. Our compounds of interest could not be imaged using MALDI due to their molecular structures, and thus the distribution of metabolites in root samples could not be measured as proposed. What opportunities for training and professional development has the project provided?In order to train for my professional development, I have met bi-weekly with my advisor, Philipp Zerbe. I have presented my work at the ASPB Conference, as well as the ASTA Conference and via iBiology, an online lecture repository. Furthermore, I participated in the writing and editing of three publications that have all been published. I have continued to attend Seed Central Networking events to expand my professional network. How have the results been disseminated to communities of interest?Results have been disseminated to scientific communities of interest via three peer-reviewed publications, two conference presentations, and an online recorded talk. I promoted and shared these via LinkedIn, my personal website, and Twitter. Results were disseminated to the general public via my webpage, where I have summarized the publications, and via social media. What do you plan to do during the next reporting period to accomplish the goals?In the next reporting period, I plan to continue experimental work regarding the biological activities of dolabralexins, as has been proposed, and including a new collaboration with the Topp lab at the Donald Danforth Plant Science Center. I will continue my professional development and dissemination of research through written publications, public talks, and conference attendance and presentation.
Impacts
What was accomplished under these goals?
I have made significant progress towards the project goals, including Elucidating the formation and distribution of dolabralexins in maize roots, defining the biological activities in maize pathogen defense, and assessing the defensive potential in other species. This includes experimentation using MALDI imaging (Aim 1), results regarding the structure-function relationship and species-specificity of maize diterpenoids using in vitro fungal assays (Aim 2), and attempted to generate transgenic tomato plants (Aim 3)
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2020
Citation:
Murphy, Katherine M., and Philipp Zerbe. "Specialized diterpenoid metabolism in monocot crops: Biosynthesis and chemical diversity." Phytochemistry 172 (2020): 112289.
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Ding, Yezhang, et al. "Multiple genes recruited from hormone pathways partition maize diterpenoid defences." Nature plants 5.10 (2019): 1043-1056.
- Type:
Journal Articles
Status:
Published
Year Published:
2019
Citation:
Murphy, Katherine M., et al. "A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products." Journal of visualized experiments: JoVE 152 (2019).
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
ASPB Plant Biology Conference. San Jose, CA. Lightening Talk, Oral and Poster Presentation. July 2019
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2019
Citation:
NAIST Symposium and Student Workshop, Nara, Japan. Oral Presentation. Nov 2019
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2020
Citation:
American Seed Trade Association. Monterey, CA. Poster Presentation. Feb 2020
- Type:
Other
Status:
Other
Year Published:
2019
Citation:
iBiology Young Scientist Seminar Series. San Francisco, CA. Oral Presentation. June 2019
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