Source: PENNSYLVANIA STATE UNIVERSITY submitted to NRP
DIFFERENCES IN TELOMERE LENGTHS ACROSS DAIRY CATTLE BREED GROUPS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1019200
Grant No.
2019-67011-29549
Cumulative Award Amt.
$110,128.00
Proposal No.
2018-07724
Multistate No.
(N/A)
Project Start Date
May 1, 2019
Project End Date
Apr 30, 2022
Grant Year
2019
Program Code
[A7101]- AFRI Predoctoral Fellowships
Recipient Organization
PENNSYLVANIA STATE UNIVERSITY
408 Old Main
UNIVERSITY PARK,PA 16802-1505
Performing Department
Animal Science
Non Technical Summary
Dairy cattle health and wellbeing are of critical concern to both consumers and farmers. Telomeres are the repetitive sequence at chromosome ends and telomere length (TL) is related to disease and stress in humans. TL may serve as an indicator of wellbeing in livestock species. Early studies in dairy cattle have focused on the Holstein breed. However, differences may be present between dairy cattle breeds and results from Holstein studies may not be applicable to other breeds. The current project will assess differences in telomere length between Holsteins, Jerseys, Montbeliarde, Holstein x Jersey crosses, and Holstein x Montbeliarde crosses. It will also attempt to establish relationships with on farm cow performance and wellbeing. The results will aid in the establishment of TL as a biomarker of animal wellbeing.
Animal Health Component
80%
Research Effort Categories
Basic
20%
Applied
80%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
30334991081100%
Knowledge Area
303 - Genetic Improvement of Animals;

Subject Of Investigation
3499 - Dairy cattle, general/other;

Field Of Science
1081 - Breeding;
Goals / Objectives
The overall goal of this project is to determine breed differences and hybrid vigor for telomere length (TL) and TL variaion in dairy cattle. Three objectives are planned to accomplish this goal. 1) Estimate TL and annual TL change among breed groups and determine effects of heterosis. 2) Establish relationships of TL with cow performance and measures of well-being within breed groups. 3) Determine relationships between genomic predicted transmitting abilities and TL for Holsteins and Jerseys.
Project Methods
Objective 1: Whole blood will be collected from cows at the beginning of year 1 and year 2. After DNA extraction, telomere length will be estimated using multiplex qPCR. Five breed groups will be used which include pure Holsteins, pure Jerseys, pure Montbeliardes, F1 Jersey x Holstein crosses, and F1 Montbeliarde x Holstein crosses. Each breed group will include approximately equal portions of nulliparous, primiparous, and multiparous animals. A multiple regression model will be used to compare telomere length and annual change in telomere length. Heterosis will be calculated as the mean of the crossbred group minus the mean of the purebred parent lines for both telomere length and telomere length change. Objective 2: Cows will be visually appraised during each herd visit for body condition score, hygiene, and locomotion. Cow data for production, fertility, and udder health will also be collected. These traits will be evaluated for relationships with telomere length and telomere length change using multiple regression techniques.Objective 3: Purebred Holsteins and Jerseys will be genotyped and receive official genomic predicted transmitting abilites for production, fertility and health traits evaluated through the Council on Dairy Cattle breeding. Pearson correlations will be used to establish correlations between telomere length and genomic predicted transmitting abilities.

Progress 05/01/21 to 04/30/22

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?An abstract was submitted to the ADSA Annual Meeting for the summer of 2022 relating to this project which will allow the results to be dissemiated to the animal breeding community. What do you plan to do during the next reporting period to accomplish the goals?The final results will be summarized in a research paper to be submitted to a peer-reviewed journal.

Impacts
What was accomplished under these goals? We were able to estimate TL and estimate breed averages. We also were able to estimated breed differences for production and conformation traits related to performance and well-being.

Publications

  • Type: Conference Papers and Presentations Status: Accepted Year Published: 2022 Citation: Haagen, I.W., T. Muraturi, and C. Dechow. 2022. Breed differences for telomere length, body condition score, stature, and production between Holsteins, Montbeliardes, and their crosses. ADSA.


Progress 05/01/19 to 04/30/22

Outputs
Target Audience:The target audience is the scientific community and dairy producers. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The PI was able to attend the American Dairy Science Association meeting at present research to broad dairy science audience. How have the results been disseminated to communities of interest?We have presented the results of this project at a national conference and engaged with producers who participated in our research. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? This was the first project in the United States to estimate breed differences and hybrid vigor for telomere length as well as production traits. We observed no statistical difference in telomere length between breeds; however, there were clear differences for production and conformation traits. In particular, Montbeliarde cows were produced less milk yield than either Holsteins or F1 Montbeliarde x Holstein crosses. Montbeliarde cows also produced less fat than Holsteins and less protein than F1 crosses. Finally, beneficial changes, such as decreased stature and increased body condition, were observed for F1 crosses when compared to Holsteins.

Publications


    Progress 05/01/20 to 04/30/21

    Outputs
    Target Audience:The primary audience has been farm operators during data collection. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period, final data analyses will occur and manuscript submitted for consideration in a peer-reviewed journal.

    Impacts
    What was accomplished under these goals? We have successfully completed all farm visits and DNA extractions for telomere length estimation.

    Publications


      Progress 05/01/19 to 04/30/20

      Outputs
      Target Audience:The target audience is researchers, dairy(wo)men, breed associations, and AI company employees. So far, we have primarily interacted with farm managers through data collection. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?The PD was able to teach substantial portions of an Animal Science course during the Spring 2020 and in addition was a teaching assistant for another course during the Fall 2019. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals?We plan on visiting our participating herds once more during the Summer/Fall of 2020. During these visits, we will collect additional blood samples and once again appraise cow phenotypes.Additionally during the next reporting period, we will complete lab work (DNA extraction and qPCR). Once all lab work is completed, we will be able to determine differences in TL, associateTL with cow performance and well-being measures, and determine the relationship of TL phenotypes with genetic traits currently evaluated in the United States.

      Impacts
      What was accomplished under these goals? Objective 1) We were able to identify and visit participating herds to collect bloodsamples and evaluate cow phenotypes. We have started the process ofextracting DNA for TL estimation which will be used to determine breed differencs. Objective 2) Currently, we are working to standardize farm data to complete this objective. 3.) This objective hinges on the completion of objective 1. As such, we are actively extracting DNA to estimate TL.

      Publications